BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0336 (559 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g62010.1 68416.m06964 expressed protein 29 2.8 At2g12880.1 68415.m01404 zinc knuckle (CCHC-type) family protein... 29 2.8 At3g04810.1 68416.m00520 protein kinase, putative similar to LST... 28 4.9 At1g14780.1 68414.m01767 expressed protein 27 6.4 At3g50000.1 68416.m05467 casein kinase II alpha chain 2 identica... 27 8.5 At3g12280.1 68416.m01533 retinoblastoma-related protein (RBR1) n... 27 8.5 At3g10440.1 68416.m01252 hypothetical protein 27 8.5 At3g05470.1 68416.m00599 formin homology 2 domain-containing pro... 27 8.5 >At3g62010.1 68416.m06964 expressed protein Length = 1254 Score = 28.7 bits (61), Expect = 2.8 Identities = 11/28 (39%), Positives = 19/28 (67%) Frame = -3 Query: 278 ARAKARVLAYWEDRPGQELKSPAEIMFA 195 A ++RV+ YWE+ + +K+PAE+ A Sbjct: 1018 ASNQSRVIGYWENIQPENVKTPAEVYAA 1045 >At2g12880.1 68415.m01404 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 119 Score = 28.7 bits (61), Expect = 2.8 Identities = 12/40 (30%), Positives = 19/40 (47%) Frame = -1 Query: 277 REQKRESWRTGKTGQARSSNLPPK*CLRRDKIGLSSRQCH 158 R+ RE R R ++ P+ C + K+G +R CH Sbjct: 11 RDNDRERTRASYNNDRRRNDYDPRACYKCGKLGHFARSCH 50 >At3g04810.1 68416.m00520 protein kinase, putative similar to LSTK-1-like kinase [Lycopersicon esculentum] GI:15637110; contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 606 Score = 27.9 bits (59), Expect = 4.9 Identities = 17/55 (30%), Positives = 20/55 (36%) Frame = -1 Query: 187 KIGLSSRQCHSRVSCVRWPNDVRNRPEDFPQFSTLCRGRQHSPAPQRSGNTAETS 23 KI L S V +WP R FP+ G+ HS P R E S Sbjct: 264 KIHLKVNDPGSNVLPAQWPESESARRNSFPEQRRRPAGKSHSFGPSRFRGNLEDS 318 >At1g14780.1 68414.m01767 expressed protein Length = 627 Score = 27.5 bits (58), Expect = 6.4 Identities = 12/25 (48%), Positives = 13/25 (52%) Frame = -1 Query: 133 PNDVRNRPEDFPQFSTLCRGRQHSP 59 P N+ F S LCRG QHSP Sbjct: 568 PVPANNKIVKFVDLSQLCRGPQHSP 592 >At3g50000.1 68416.m05467 casein kinase II alpha chain 2 identical to casein kinase II, alpha chain 2 (CK II) [Arabidopsis thaliana] SWISS-PROT:Q08466 Length = 403 Score = 27.1 bits (57), Expect = 8.5 Identities = 13/45 (28%), Positives = 22/45 (48%) Frame = +1 Query: 163 TVEKRAQSYLDANIISAGDLSSWPGLSSQYAKTRAFALAEKSKRG 297 TV +A+ Y D N+I D + L+ Q+ + + + K RG Sbjct: 69 TVMSKARVYTDVNVIRPKDYWDYESLNVQWGEQDDYEVVRKVGRG 113 >At3g12280.1 68416.m01533 retinoblastoma-related protein (RBR1) nearly identical to retinoblastoma-related protein [Arabidopsis thaliana] GI:8777927; contains Pfam profiles: PF01858 retinoblastoma-associated protein A domain, PF01857 retinoblastoma-associated protein B domain Length = 1013 Score = 27.1 bits (57), Expect = 8.5 Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 4/39 (10%) Frame = -2 Query: 222 QISRRNNVCVEIRLGSLLDSVIAE----YHACVGQMTSA 118 ++S +NV V GS +D++I+ Y+ACVG+ T A Sbjct: 901 KVSAVHNVYVSPLRGSKMDALISHSTKSYYACVGESTHA 939 >At3g10440.1 68416.m01252 hypothetical protein Length = 556 Score = 27.1 bits (57), Expect = 8.5 Identities = 13/35 (37%), Positives = 15/35 (42%) Frame = -1 Query: 130 NDVRNRPEDFPQFSTLCRGRQHSPAPQRSGNTAET 26 ND N D Q C GRQ S + R + ET Sbjct: 235 NDAENHINDNVQSKRYCAGRQSSSSKTREASQTET 269 >At3g05470.1 68416.m00599 formin homology 2 domain-containing protein / FH2 domain-containing protein contains formin homology 2 domain, Pfam:PF02181 Length = 884 Score = 27.1 bits (57), Expect = 8.5 Identities = 17/42 (40%), Positives = 20/42 (47%) Frame = +3 Query: 117 LRTSFGQRTHDTLL*HCREESPILSRRKHYFGGRFELLAWPV 242 LR QRTH E P + RRKH GG F+ + PV Sbjct: 128 LRRPLPQRTHPL-------EQPEIQRRKHEKGGTFKKILVPV 162 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,474,258 Number of Sequences: 28952 Number of extensions: 199329 Number of successful extensions: 493 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 489 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 493 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1062855648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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