BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0332 (759 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 301 5e-83 SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 27 2.2 SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 27 2.9 SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac... 26 6.7 SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 26 6.7 SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces po... 25 8.9 >SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 418 Score = 301 bits (740), Expect = 5e-83 Identities = 137/215 (63%), Positives = 170/215 (79%), Gaps = 2/215 (0%) Frame = +1 Query: 121 KRVVAAKPVVEMDGDEMTRIIWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 300 +++ PVVEMDGDEMTR+IW I+E+L+ PY+ + Y+DLG+ RD T+DQ+T+D+A Sbjct: 12 QKITVKNPVVEMDGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAA 71 Query: 301 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 480 AILK++VGIKCATITPDE RV+E+ LKKMW SPNGTIRNIL GTVFREPIL ++IP+ + Sbjct: 72 KAILKNDVGIKCATITPDEARVKEYNLKKMWKSPNGTIRNILNGTVFREPILIKNIPKYI 131 Query: 481 PGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVELVYTTQDGTTERRV--LYDFKTPGVAMG 654 PGWT PI IGRHA GDQYK+ D V PGK+EL +T + + + +Y+F GVAM Sbjct: 132 PGWTNPICIGRHAFGDQYKSTDLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMS 191 Query: 655 MYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTIL 759 MYNTD+SIR FAHSSFQ+ALQKK PLYLSTKNTIL Sbjct: 192 MYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTIL 226 >SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 27.5 bits (58), Expect = 2.2 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +1 Query: 274 DDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWL 396 DD VT + AIL+H V + P E+ V E + ++W+ Sbjct: 56 DDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWM 96 >SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 27.1 bits (57), Expect = 2.9 Identities = 16/34 (47%), Positives = 19/34 (55%) Frame = -2 Query: 713 KAT*KLEWAKDRIDSSVLYMPIATPGVLKSYNTR 612 KAT K EW K++ID L I T LK+Y R Sbjct: 300 KATEKREWVKEKIDLESLLGTIQTN--LKTYQLR 331 >SPBC1539.09c |trp1||anthranilate synthase component II|Schizosaccharomyces pombe|chr 2|||Manual Length = 759 Score = 25.8 bits (54), Expect = 6.7 Identities = 17/57 (29%), Positives = 29/57 (50%) Frame = +1 Query: 37 RNMANTSTTKILKCVNQFASLTRNYGTAKRVVAAKPVVEMDGDEMTRIIWAKIKERL 207 R + N S+++I L + GT + ++AAK +VE GD + I K K ++ Sbjct: 516 RELLNLSSSEISNGKKTSTPLVKVCGT-RSLLAAKTIVESGGDLIGLIFVEKSKRKV 571 >SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 490 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/21 (57%), Positives = 15/21 (71%) Frame = -2 Query: 218 YGNISLSLILAQMMRVISSPS 156 YGNI+L LI A + R I SP+ Sbjct: 201 YGNITLGLIRALLAREILSPA 221 >SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 434 Score = 25.4 bits (53), Expect = 8.9 Identities = 15/41 (36%), Positives = 23/41 (56%) Frame = -2 Query: 428 PKMLRIVPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRM 306 P L ++ GLSH F N+ + TL S VA+ + +L R+ Sbjct: 223 PVFLNLIYNGLSHFFQNILAFTLLSIISPVAYSIASLIKRI 263 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,285,576 Number of Sequences: 5004 Number of extensions: 68522 Number of successful extensions: 187 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 177 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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