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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0332
         (759 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom...   301   5e-83
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    27   2.2  
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ...    27   2.9  
SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac...    26   6.7  
SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc...    26   6.7  
SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces po...    25   8.9  

>SPAC6G10.08 |idp1||isocitrate dehydrogenase
           Idp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 418

 Score =  301 bits (740), Expect = 5e-83
 Identities = 137/215 (63%), Positives = 170/215 (79%), Gaps = 2/215 (0%)
 Frame = +1

Query: 121 KRVVAAKPVVEMDGDEMTRIIWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 300
           +++    PVVEMDGDEMTR+IW  I+E+L+ PY+ +   Y+DLG+  RD T+DQ+T+D+A
Sbjct: 12  QKITVKNPVVEMDGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAA 71

Query: 301 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 480
            AILK++VGIKCATITPDE RV+E+ LKKMW SPNGTIRNIL GTVFREPIL ++IP+ +
Sbjct: 72  KAILKNDVGIKCATITPDEARVKEYNLKKMWKSPNGTIRNILNGTVFREPILIKNIPKYI 131

Query: 481 PGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVELVYTTQDGTTERRV--LYDFKTPGVAMG 654
           PGWT PI IGRHA GDQYK+ D V   PGK+EL +T +   + +    +Y+F   GVAM 
Sbjct: 132 PGWTNPICIGRHAFGDQYKSTDLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMS 191

Query: 655 MYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTIL 759
           MYNTD+SIR FAHSSFQ+ALQKK PLYLSTKNTIL
Sbjct: 192 MYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTIL 226


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +1

Query: 274 DDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWL 396
           DD VT   + AIL+H V     +  P E+ V E +  ++W+
Sbjct: 56  DDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWM 96


>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 621

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 16/34 (47%), Positives = 19/34 (55%)
 Frame = -2

Query: 713 KAT*KLEWAKDRIDSSVLYMPIATPGVLKSYNTR 612
           KAT K EW K++ID   L   I T   LK+Y  R
Sbjct: 300 KATEKREWVKEKIDLESLLGTIQTN--LKTYQLR 331


>SPBC1539.09c |trp1||anthranilate synthase component
           II|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 759

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 17/57 (29%), Positives = 29/57 (50%)
 Frame = +1

Query: 37  RNMANTSTTKILKCVNQFASLTRNYGTAKRVVAAKPVVEMDGDEMTRIIWAKIKERL 207
           R + N S+++I         L +  GT + ++AAK +VE  GD +  I   K K ++
Sbjct: 516 RELLNLSSSEISNGKKTSTPLVKVCGT-RSLLAAKTIVESGGDLIGLIFVEKSKRKV 571


>SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 490

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = -2

Query: 218 YGNISLSLILAQMMRVISSPS 156
           YGNI+L LI A + R I SP+
Sbjct: 201 YGNITLGLIRALLAREILSPA 221


>SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 434

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 15/41 (36%), Positives = 23/41 (56%)
 Frame = -2

Query: 428 PKMLRIVPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRM 306
           P  L ++  GLSH F N+ + TL S    VA+ + +L  R+
Sbjct: 223 PVFLNLIYNGLSHFFQNILAFTLLSIISPVAYSIASLIKRI 263


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,285,576
Number of Sequences: 5004
Number of extensions: 68522
Number of successful extensions: 187
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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