Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0332
(759 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 316 8e-87
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 312 2e-85
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 309 1e-84
At5g49680.1 68418.m06151 cell expansion protein, putative simila... 29 4.4
At2g38710.1 68415.m04754 AMMECR1 family similar to AMMECR1 (GI:6... 29 4.4
At2g33200.1 68415.m04067 F-box family protein contains F-box dom... 29 4.4
At4g15480.1 68417.m02366 UDP-glucoronosyl/UDP-glucosyl transfera... 28 5.9
At5g28740.1 68418.m03530 transcription-coupled DNA repair protei... 28 7.7
At4g38730.1 68417.m05486 expressed protein 28 7.7
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 316 bits (777), Expect = 8e-87
Identities = 144/217 (66%), Positives = 174/217 (80%), Gaps = 1/217 (0%)
Frame = +1
Query: 112 GTAKRVVAAKPVVEMDGDEMTRIIWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTI 291
G + R+ P+VEMDGDEMTR+IW+ IKE+LI PY+ +D YFDLG+ +RDATDD+VT+
Sbjct: 71 GGSDRIQVQNPIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTV 130
Query: 292 DSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIP 471
+SA A LK+NV IKCATITPDE RV+EF LK MW SPNGTIRNIL GTVFREPI+C +IP
Sbjct: 131 ESAEAALKYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIP 190
Query: 472 RVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVELVYTTQDGTTERRV-LYDFKTPGVA 648
R+VPGW KPI IGRHA GDQY+A D V+ PGK+++V+ +DG + +YDFK PGVA
Sbjct: 191 RLVPGWEKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVA 250
Query: 649 MGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTIL 759
+ MYN DESIR+FA SS +AL KKWPLYLSTKNTIL
Sbjct: 251 LAMYNVDESIRAFAESSMAMALTKKWPLYLSTKNTIL 287
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 312 bits (765), Expect = 2e-85
Identities = 141/213 (66%), Positives = 170/213 (79%)
Frame = +1
Query: 121 KRVVAAKPVVEMDGDEMTRIIWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 300
+++ A P+VEMDGDEMTR+IW IK++LI P+V++D YFDLGLPHRDATDD+VTI+SA
Sbjct: 4 EKIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESA 63
Query: 301 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 480
A K+NV IKCATITPDE RV EF LK+MW SPNGTIRNIL GTVFREPI+C+++P++V
Sbjct: 64 EATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLV 123
Query: 481 PGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVELVYTTQDGTTERRVLYDFKTPGVAMGMY 660
PGWTKPI IGRHA GDQY+A D V+ PGK+ + + +DG TE V GVAM MY
Sbjct: 124 PGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMY 183
Query: 661 NTDESIRSFAHSSFQVALQKKWPLYLSTKNTIL 759
NTDESIR+FA +S A +KKWPLYLSTKNTIL
Sbjct: 184 NTDESIRAFADASMNTAYEKKWPLYLSTKNTIL 216
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 309 bits (759), Expect = 1e-84
Identities = 141/214 (65%), Positives = 173/214 (80%), Gaps = 1/214 (0%)
Frame = +1
Query: 121 KRVVAAKPVVEMDGDEMTRIIWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 300
+++ PVVEMDGDEMTR+IW IK++LIFP++++D YFDLGLP+RD TDD+VTI++A
Sbjct: 4 EKIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETA 63
Query: 301 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 480
A LK+NV IKCATITPDE RV EF LKKMW SPNGTIRNIL GTVFREPI+C++IPR+V
Sbjct: 64 EATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLV 123
Query: 481 PGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVELVYTTQDGTTERRV-LYDFKTPGVAMGM 657
PGWTKPI IGRHA GDQY+A D +V +PGK++LV+ + + +++F GVA+ M
Sbjct: 124 PGWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAM 183
Query: 658 YNTDESIRSFAHSSFQVALQKKWPLYLSTKNTIL 759
YNTDESIR+FA SS A QKKWPLYLSTKNTIL
Sbjct: 184 YNTDESIRAFAESSMYTAYQKKWPLYLSTKNTIL 217
>At5g49680.1 68418.m06151 cell expansion protein, putative similar to
SABRE [Arabidopsis thaliana] GI:719291
Length = 1378
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/61 (22%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +1
Query: 166 EMTRIIWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNV-GIKCAT 342
++ ++IW+ + + F V++D + +D +RD Q T + + +L++ + KC T
Sbjct: 904 QINKVIWSMVLDGKTFAEVEIDNMIYDFNRDYRDIGIAQFT--TRYVVLRNCLPNAKCDT 961
Query: 343 I 345
+
Sbjct: 962 V 962
>At2g38710.1 68415.m04754 AMMECR1 family similar to AMMECR1
(GI:6063688) [Homo sapiens]; contains Pfam profile
PF01871: AMMECR1
Length = 214
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -3
Query: 751 YSSWTGIEAIFSVKLLENWNGQRTESIRR 665
+ WT IEAI S+ +NG TE++RR
Sbjct: 143 HEGWTKIEAIDSLVRKAGYNGVITEAVRR 171
>At2g33200.1 68415.m04067 F-box family protein contains F-box domain
Pfam:PF00646
Length = 376
Score = 28.7 bits (61), Expect = 4.4
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +3
Query: 378 VEENVAKSERNDP*HFGRYSIPRTDIVSEYPEGCAWLDE 494
+E ++ ER + ++ I RTDI+S + C W++E
Sbjct: 119 MESSILGKERLEKEVAWKHFIERTDIISTKKQACLWINE 157
>At4g15480.1 68417.m02366 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 490
Score = 28.3 bits (60), Expect = 5.9
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 1/31 (3%)
Frame = -2
Query: 716 CKAT*K-LEWAKDRIDSSVLYMPIATPGVLK 627
CK+T K LEW R SSV+Y+ T LK
Sbjct: 271 CKSTDKCLEWLDSRPKSSVVYISFGTVAYLK 301
>At5g28740.1 68418.m03530 transcription-coupled DNA repair
protein-related similar to XAB2 (XPA-binding protein 2)
[Homo sapiens] GI:10566459
Length = 917
Score = 27.9 bits (59), Expect = 7.7
Identities = 24/69 (34%), Positives = 33/69 (47%), Gaps = 8/69 (11%)
Frame = +1
Query: 85 QFASLTRNYGTAKRVV-----AAKPVVEMDGDEMTRIIWAKIKERLIFPYVKVDCLY--- 240
Q+A L +YG AKR + A K V E EM I ++ E IF + +Y
Sbjct: 629 QYAKLEEDYGLAKRAMKVYEEATKKVPEGQKLEMYEIYISRAAE--IFGVPRTREIYEQA 686
Query: 241 FDLGLPHRD 267
+ GLPH+D
Sbjct: 687 IESGLPHKD 695
>At4g38730.1 68417.m05486 expressed protein
Length = 326
Score = 27.9 bits (59), Expect = 7.7
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = -3
Query: 754 WYSSWTGIEAIFSVKLLENWNGQRTESI 671
+Y +T + + S + ++WNGQ T+SI
Sbjct: 246 YYVMFTTLTIVASAIMFKDWNGQNTDSI 273
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,250,864
Number of Sequences: 28952
Number of extensions: 364839
Number of successful extensions: 858
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 856
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -