Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0321
         (640 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholi...    24   1.1  
DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholi...    23   1.9  
EF625898-1|ABR45905.1|  686|Apis mellifera hexamerin protein.          22   5.8  
DQ026038-1|AAY87897.1|  520|Apis mellifera nicotinic acetylcholi...    21   7.6  

>DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 24.2 bits (50), Expect = 1.1
 Identities = 23/105 (21%), Positives = 46/105 (43%)
 Frame = +2

Query: 38  LNKKRKIIRMALAFRGVIRNVARRLQVRNYADAPKGDEMALTFAAGNKVFYDKQVVKQID 217
           L+   K++R  +    V+R V  +L++    D    +++  T     + +YD ++  +  
Sbjct: 37  LSNYNKLVRPVVNTSDVLR-VCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWE-- 93

Query: 218 VPSFSGAFGILPKHVPTLAVLRPGVVTILENDGKQNKIFVSSGTI 352
            P   G  G+   HVP+  + RP +V     DG      ++  T+
Sbjct: 94  -PKEYG--GVKMLHVPSDHIWRPDIVLYNNADGNYEVTLMTKATV 135


>DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 23.4 bits (48), Expect = 1.9
 Identities = 24/105 (22%), Positives = 45/105 (42%)
 Frame = +2

Query: 38  LNKKRKIIRMALAFRGVIRNVARRLQVRNYADAPKGDEMALTFAAGNKVFYDKQVVKQID 217
           L+   K++R  +    V+R V  +L++    D    +++  T     + +YD ++  +  
Sbjct: 37  LSNYNKLVRPVVNTSDVLR-VCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWE-- 93

Query: 218 VPSFSGAFGILPKHVPTLAVLRPGVVTILENDGKQNKIFVSSGTI 352
            P   G  G+   HVP+  + RP +V     DG       +  TI
Sbjct: 94  -PKEYG--GVKMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATI 135


>EF625898-1|ABR45905.1|  686|Apis mellifera hexamerin protein.
          Length = 686

 Score = 21.8 bits (44), Expect = 5.8
 Identities = 15/48 (31%), Positives = 21/48 (43%)
 Frame = -1

Query: 220 YINLFDNLFVIKDLITRSEGQCHFVTFGSICIVTYLKPTSYISDYTTE 77
           Y N F +  VI++       +   V  G   I TY+  T+Y S Y  E
Sbjct: 166 YPNYFFDSSVIEEAQNLKMSRGSSVVTGMNNIETYIVNTNYSSKYMRE 213


>DQ026038-1|AAY87897.1|  520|Apis mellifera nicotinic acetylcholine
           receptor beta1subunit protein.
          Length = 520

 Score = 21.4 bits (43), Expect = 7.6
 Identities = 8/15 (53%), Positives = 12/15 (80%)
 Frame = +2

Query: 224 SFSGAFGILPKHVPT 268
           ++SG+   LPKH+PT
Sbjct: 371 TYSGSPTELPKHLPT 385


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 145,853
Number of Sequences: 438
Number of extensions: 2556
Number of successful extensions: 8
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19193721
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)