Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0319
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 287 1e-78
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 266 2e-72
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 32 0.068
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.1
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 26 4.5
SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr... 26 5.9
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 5.9
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 287 bits (704), Expect = 1e-78
Identities = 140/230 (60%), Positives = 173/230 (75%), Gaps = 1/230 (0%)
Frame = +1
Query: 4 QIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNI 183
QI+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GKGVL A+ N+
Sbjct: 10 QIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGKGVLKAVGNV 69
Query: 184 NELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXXXXXXXXNVP 363
N +IAP + KANL+VT Q+ DE +LKLDGTENKSKLGANAILGVS+ +P
Sbjct: 70 NNIIAPAVVKANLDVTDQKAADEFLLKLDGTENKSKLGANAILGVSMAICRAGAAQKKLP 129
Query: 364 LYKHLADLAG-NNDIVLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEAMRMGSEV 540
L+K++A+ G VLPVP+FNV+NGGSHAG LA QEFMI PTGA +FSEAMR G+E
Sbjct: 130 LWKYIAENFGTKGPYVLPVPSFNVLNGGSHAGGDLAFQEFMILPTGAPSFSEAMRWGAET 189
Query: 541 YHHLKKIIKEKFGLDSTAVGDESGFAPNIQNNKDALYLIQDAIQKAGYAG 690
YH LK I K+++G + VGDE G AP++Q ++AL LI +AI KAGY G
Sbjct: 190 YHTLKSIAKKRYGSSAGNVGDEGGIAPDLQTPQEALDLIVEAINKAGYEG 239
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 266 bits (653), Expect = 2e-72
Identities = 127/229 (55%), Positives = 167/229 (72%), Gaps = 1/229 (0%)
Frame = +1
Query: 7 IFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNIN 186
I+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV A+ N+N
Sbjct: 12 IYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGVTIAVHNVN 71
Query: 187 ELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXXXXXXXXNVPL 366
+I P L K+++++T QR IDE M+KLDGT +KSKLGAN+I+GVS+ +PL
Sbjct: 72 NIIGPALVKSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMAVARAAAAFLKIPL 131
Query: 367 YKHLADLAGNNDI-VLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEAMRMGSEVY 543
Y+++ LAG+ +PVP+FNV+NGG HAG LA QEFMI P A TFSE +R GSEVY
Sbjct: 132 YEYIGKLAGSKTTECIPVPSFNVLNGGRHAGGDLAFQEFMIMPIKAPTFSEGLRWGSEVY 191
Query: 544 HHLKKIIKEKFGLDSTAVGDESGFAPNIQNNKDALYLIQDAIQKAGYAG 690
H LK + K+K+G + VGDE G AP++ ++AL L+ +AI++AGY G
Sbjct: 192 HTLKALAKKKYGASAGNVGDEGGIAPDLTTAEEALDLVNEAIKEAGYDG 240
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 32.3 bits (70), Expect = 0.068
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = -2
Query: 530 PILMASLKVDAPVGKIMNSCMASLFPACDPPLITLKAGTGRTISL 396
PI+ S + + +G+ +N + LFP+CD LI G TI L
Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCDKFLIVKPVIHGITIFL 236
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 28.3 bits (60), Expect = 1.1
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +1
Query: 13 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINEL 192
D +G +VD ++A +ST LE R N + E V TAI + E
Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198
Query: 193 IAPELTKANLEV 228
ELT+ N+E+
Sbjct: 199 FEGELTEDNIEI 210
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 26.2 bits (55), Expect = 4.5
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -1
Query: 285 FVLSAIQLKHEFINLSLLGYFKVGFG 208
F +S+I+ KH++IN+ Y +G G
Sbjct: 113 FSISSIKTKHDYINIIKKHYVSLGVG 138
>SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 5.9
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +3
Query: 321 PSCC*GWCCQEK 356
PSCC G CC+E+
Sbjct: 490 PSCCGGHCCKEE 501
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 5.9
Identities = 18/77 (23%), Positives = 34/77 (44%)
Frame = +1
Query: 22 GNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINELIAP 201
G P + ++ LG F + S S + ++ + + +E H + K +NE +A
Sbjct: 1109 GEPILTQQVIQNLGGFSSEEVSMVSRCIRSRTQIMNMLATEIHYAASVGQNKYLNEYVA- 1167
Query: 202 ELTKANLEVTQQREIDE 252
L + N E T E+ +
Sbjct: 1168 SLIRTN-EKTHSTELSQ 1183
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.314 0.134 0.371
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,883,584
Number of Sequences: 5004
Number of extensions: 56848
Number of successful extensions: 107
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 103
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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