Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0316
(742 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 452 e-129
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 443 e-126
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 334 7e-94
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 301 6e-84
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 40 3e-05
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 25 0.75
AM158085-1|CAJ43389.1| 171|Apis mellifera globin 1 protein. 24 1.7
AM158084-1|CAJ43388.1| 171|Apis mellifera globin 1 protein. 24 1.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.0
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.0
DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex det... 22 5.3
DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 21 9.2
AB193550-1|BAD66824.1| 699|Apis mellifera soluble guanylyl cycl... 21 9.2
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 452 bits (1114), Expect = e-129
Identities = 210/220 (95%), Positives = 214/220 (97%)
Frame = +2
Query: 83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 262
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 263 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 442
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 443 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYHEARYEEIKKEVSSYIKK 622
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY E R+EEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 623 IGYNPATVAFVPISGWHGDNMLEPSDKMPWFKGWTIDRKD 742
IGYNPA VAFVPISGWHGDNMLE S KMPWFKGWT++RK+
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKE 220
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 443 bits (1091), Expect = e-126
Identities = 205/220 (93%), Positives = 212/220 (96%)
Frame = +2
Query: 83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 262
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 263 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 442
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 443 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYHEARYEEIKKEVSSYIKK 622
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPY EAR+EEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 623 IGYNPATVAFVPISGWHGDNMLEPSDKMPWFKGWTIDRKD 742
IGYN A+VAFVPISGWHGDNMLEPS K PW+KGW ++RKD
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKD 220
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 334 bits (820), Expect = 7e-94
Identities = 154/163 (94%), Positives = 158/163 (96%)
Frame = +2
Query: 254 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 433
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 434 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYHEARYEEIKKEVSSY 613
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY E R+EEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 614 IKKIGYNPATVAFVPISGWHGDNMLEPSDKMPWFKGWTIDRKD 742
IKKIGYNPA VAFVPISGWHGDNMLE S KMPWFKGWT++RK+
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKE 163
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 301 bits (738), Expect = 6e-84
Identities = 138/147 (93%), Positives = 142/147 (96%)
Frame = +2
Query: 302 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 481
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 482 REHALLAFTLGVKQLIVGVNKMDSTEPPYHEARYEEIKKEVSSYIKKIGYNPATVAFVPI 661
REHALLAFTLGVKQLIVGVNKMDSTEPPY E R+EEIKKEVSSYIKKIGYNPA VAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 662 SGWHGDNMLEPSDKMPWFKGWTIDRKD 742
SGWHGDNMLE S KMPWFKGWT++RK+
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKE 147
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 39.5 bits (88), Expect = 3e-05
Identities = 37/117 (31%), Positives = 52/117 (44%)
Frame = +2
Query: 341 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 520
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 521 QLIVGVNKMDSTEPPYHEARYEEIKKEVSSYIKKIGYNPATVAFVPISGWHGDNMLE 691
+IV +NK+D + +YE K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.56
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 95 KTHINIVVIGHVDSGKST 148
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 25.0 bits (52), Expect = 0.75
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 59 LRNLDPQXMGKEKTHINIVVIGHVDSGKST 148
L L + + ++ T INI IGHV GKST
Sbjct: 29 LTALSREVISRQAT-INIGTIGHVAHGKST 57
>AM158085-1|CAJ43389.1| 171|Apis mellifera globin 1 protein.
Length = 171
Score = 23.8 bits (49), Expect = 1.7
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -1
Query: 673 VPARYRYERHSSGIVTNFLNV 611
+PA R++ H +G++T NV
Sbjct: 76 LPANKRFQAHCAGVITALNNV 96
>AM158084-1|CAJ43388.1| 171|Apis mellifera globin 1 protein.
Length = 171
Score = 23.8 bits (49), Expect = 1.7
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -1
Query: 673 VPARYRYERHSSGIVTNFLNV 611
+PA R++ H +G++T NV
Sbjct: 76 LPANKRFQAHCAGVITALNNV 96
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.0
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 326 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 427
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 326 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 427
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex
determiner protein.
Length = 182
Score = 22.2 bits (45), Expect = 5.3
Identities = 8/32 (25%), Positives = 16/32 (50%)
Frame = +2
Query: 566 YHEARYEEIKKEVSSYIKKIGYNPATVAFVPI 661
Y+ + Y ++Y KK+ YN + +P+
Sbjct: 89 YNYSNYNNYNNNYNNYNKKLYYNINYIEQIPV 120
>DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated
ion channel subunit protein.
Length = 510
Score = 21.4 bits (43), Expect = 9.2
Identities = 16/62 (25%), Positives = 24/62 (38%)
Frame = -1
Query: 550 VHFIHADNKLFYAKSESKQGVFASLSILTDTGFELASSGCHDQYCAISLGSTRDHVLNEI 371
VH I NKL + L+I EL + Q C + LGS + + I
Sbjct: 132 VHTITVPNKLLRISQDGDILYSMRLTIKAKCPMELRNFPMDRQSCPLILGSCKCIYVQSI 191
Query: 370 SM 365
++
Sbjct: 192 NL 193
>AB193550-1|BAD66824.1| 699|Apis mellifera soluble guanylyl cyclase
alpha 1 subunit protein.
Length = 699
Score = 21.4 bits (43), Expect = 9.2
Identities = 13/43 (30%), Positives = 19/43 (44%)
Frame = -2
Query: 234 LPIS*ASFSNFSIVRLSMPPHL*MRWPVVVDFPESTWPMTTML 106
L +S N S++ L PP + R + T+P TML
Sbjct: 451 LAVSAEREKNVSLLHLIFPPDIAKRLWLGETIEAKTYPEVTML 493
Score = 21.4 bits (43), Expect = 9.2
Identities = 9/37 (24%), Positives = 17/37 (45%)
Frame = -2
Query: 570 W*GGSVESILFTPTISCFTPRVKASRACSRVCPFLLI 460
W G ++E+ + F+ V + CS P ++I
Sbjct: 477 WLGETIEAKTYPEVTMLFSDIVGFTEICSTATPMMVI 513
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 217,395
Number of Sequences: 438
Number of extensions: 5065
Number of successful extensions: 19
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23144850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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