Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0304
(678 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g17860.1 68418.m02093 cation exchanger, putative (CAX7) conta... 46 2e-05
At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 42 4e-04
At3g14070.1 68416.m01777 cation exchanger, putative (CAX9) simil... 41 9e-04
At1g54115.1 68414.m06169 cation exchanger, putative 39 0.003
At2g47600.1 68415.m05939 magnesium/proton exchanger (MHX1) ident... 31 0.93
At4g36720.1 68417.m05210 abscisic acid-responsive HVA22 family p... 29 2.8
At1g22550.1 68414.m02816 proton-dependent oligopeptide transport... 29 2.8
At4g21430.1 68417.m03097 transcription factor jumonji (jmjC) dom... 29 3.7
At5g56230.1 68418.m07017 prenylated rab acceptor (PRA1) family p... 28 4.9
At1g55640.1 68414.m06369 prenylated rab acceptor (PRA1) family p... 28 4.9
At3g09170.1 68416.m01088 Ulp1 protease family protein contains P... 28 6.5
At3g06140.1 68416.m00705 zinc finger (C3HC4-type RING finger) fa... 28 6.5
At2g13810.1 68415.m01524 aminotransferase class I and II family ... 28 6.5
At1g34610.1 68414.m04301 Ulp1 protease family protein contains P... 28 6.5
At4g28690.1 68417.m04099 expressed protein 27 8.6
At4g08410.1 68417.m01390 proline-rich extensin-like family prote... 27 8.6
At2g30800.1 68415.m03755 DEIH-box RNA/DNA helicase, putative sim... 27 8.6
>At5g17860.1 68418.m02093 cation exchanger, putative (CAX7) contains
similarity to SWISS-PROT:Q9HC58 NKX3_HUMAN
Sodium/potassium/calcium exchanger 3 precursor {Homo
sapiens}; Ca2+:Cation Antiporter (CaCA) Family member
PMID:11500563
Length = 570
Score = 46.4 bits (105), Expect = 2e-05
Identities = 37/147 (25%), Positives = 64/147 (43%), Gaps = 2/147 (1%)
Frame = +2
Query: 152 GAIVLHALISIYLFLALAVVCDKYFVPAVEKICHALNMTNDVXXXXXXXXXXXXPELFVN 331
G +VL A + + LF L YF P+++ + L ++ + P+LF +
Sbjct: 101 GHLVLSAWLFV-LFYLLGDTAASYFCPSLDSLSKVLKLSPTMAGVTLLSLGNGAPDLFSS 159
Query: 332 VIG-TFITEGDIGVGTIVGSAVF-NILAVAACCGIGAGMVVPLDWWPLTRDCLAYGITVS 505
V+ T GD G+ +I+G A F + V C + V +D RD + + +
Sbjct: 160 VVSFTRSNNGDFGLNSILGGAFFVSSFVVGTICVLIGSRDVAIDRNSFIRDVVFLLVALC 219
Query: 506 ILICIIHDEYVQWYEAFLLVSLYGVYI 586
L II V + A +S+Y +Y+
Sbjct: 220 CLGLIIFIGKVTIWVALCYLSIYLLYV 246
>At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar
to sodium/calcium exchanger protein [Mus musculus]
gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 559
Score = 41.9 bits (94), Expect = 4e-04
Identities = 33/143 (23%), Positives = 60/143 (41%), Gaps = 5/143 (3%)
Frame = +2
Query: 182 IYLFLALAVVCDKYFVPAVEKICHALNMTNDVXXXXXXXXXXXXPELFVNVIGTFITEG- 358
+ LF L +YF ++E + LN++ V P+LF +++ +F+ E
Sbjct: 100 LLLFYLLGHTASEYFCSSLESLSKLLNLSPTVAGVTLLSLGNGAPDLFASLV-SFMGESK 158
Query: 359 ---DIGVGTIVGSAVFNILAVAACCGIGA-GMVVPLDWWPLTRDCLAYGITVSILICIIH 526
D+G+ T+VG + F V I V ++ RD + + L I+
Sbjct: 159 GTYDVGLNTVVGGSGFVTCVVVGIISISLHKRRVRIERAAFIRDICFFCAAIGSLALILV 218
Query: 527 DEYVQWYEAFLLVSLYGVYICIM 595
+ ++ A SLY VY+ +
Sbjct: 219 YGKINFWGALGFCSLYAVYVAFV 241
>At3g14070.1 68416.m01777 cation exchanger, putative (CAX9) similar
to sodium/calcium exchanger protein [Mus musculus]
gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 643
Score = 40.7 bits (91), Expect = 9e-04
Identities = 45/217 (20%), Positives = 96/217 (44%), Gaps = 8/217 (3%)
Frame = +2
Query: 2 ATSSVACVALHTTPLQNMNTSERDGYIRN---CTPPAIDDFPAGLFTELQRQHGAIVLHA 172
++S+V C LH ++M +++ +++ C+P D+ + + + +I+ +
Sbjct: 86 SSSTVLCSGLH----KHMGYADQCEFLKANPICSPDGFFDYLSFFYCSCR--DFSILGYM 139
Query: 173 LISIYL---FLALAVVCDKYFVPAVEKICHALNMTNDVXXXXXXXXXXXXPELFVNVIGT 343
++ ++L F L YF ++EK+ L + V P++F ++
Sbjct: 140 MLGVWLVALFYLLGNTAADYFCCSLEKLSKLLRLPPTVAGVTLLPLGNGAPDVFASIAAF 199
Query: 344 FITE-GDIGVGTIVGSAVFNILAVAACCGI-GAGMVVPLDWWPLTRDCLAYGITVSILIC 517
T+ G++G+ +++G AVF V + A V +D RD + ++ L+
Sbjct: 200 VGTDKGEVGLNSVLGGAVFVTSVVVGIVSLCVADKEVKIDKNCFIRDLSFFLFSLVSLLV 259
Query: 518 IIHDEYVQWYEAFLLVSLYGVYICIMYYDKPIQNFAK 628
I+ V A VS+Y VY ++ + ++ AK
Sbjct: 260 ILMVGRVTVRIAIAFVSIYVVYAFLVAANVILRKHAK 296
>At1g54115.1 68414.m06169 cation exchanger, putative
Length = 644
Score = 39.1 bits (87), Expect = 0.003
Identities = 45/204 (22%), Positives = 88/204 (43%), Gaps = 8/204 (3%)
Frame = +2
Query: 8 SSVACVALHTTPLQNMNTSERDGYIRN---CTPPAIDDFPAGLFTELQ--RQHGAIVLHA 172
S+V+C LH ++ +++ ++++ C+P D+ + + + G I+L
Sbjct: 87 STVSCSGLH----EHRGYADQCEFLKSNPICSPDGFFDYLKFFYCSCRDFKILGYILLGV 142
Query: 173 LISIYLFLALAVVCDKYFVPAVEKICHALNMTNDVXXXXXXXXXXXXPELFVNVIGTFI- 349
+ + LF L YF ++EK+ L + V P++F + I F+
Sbjct: 143 WL-VALFYLLGNTAADYFCCSLEKLSKLLRLPPTVAGVTLLPLGNGAPDVFAS-IAAFVG 200
Query: 350 -TEGDIGVGTIVGSAVFNILAVAACCGI-GAGMVVPLDWWPLTRDCLAYGITVSILICII 523
+G++G+ +++G AVF V + A V +D RD + T+ L+ I+
Sbjct: 201 SDKGEVGLNSVLGGAVFVTCVVVGIVSLCVADKEVKIDKKCFIRDLSFFLFTLVALMVIL 260
Query: 524 HDEYVQWYEAFLLVSLYGVYICIM 595
V A VS+Y Y ++
Sbjct: 261 MVGKVTVGIAIAFVSIYVFYASLV 284
>At2g47600.1 68415.m05939 magnesium/proton exchanger (MHX1)
identical to magnesium/proton exchanger AtMHX
[Arabidopsis thaliana] gi|6492237|gb|AAF14229;
Ca2+:Cation Antiporter (CaCA) Family member
PMID:11500563
Length = 539
Score = 30.7 bits (66), Expect = 0.93
Identities = 27/88 (30%), Positives = 41/88 (46%), Gaps = 7/88 (7%)
Frame = +2
Query: 356 GDIGVGTIVGSAVFNILAVAACCGI--GAG---MVVPLDWW--PLTRDCLAYGITVSILI 514
G +G GT+VGSA F++ + A C + AG + L W L AY I + I++
Sbjct: 130 GGLGPGTLVGSAAFDLFPIHAVCVVVPKAGELKKISDLGVWLVELVWSFWAY-IWLYIIL 188
Query: 515 CIIHDEYVQWYEAFLLVSLYGVYICIMY 598
+ + EA L V YG+ + Y
Sbjct: 189 EVWSPNVITLVEALLTVLQYGLLLVHAY 216
>At4g36720.1 68417.m05210 abscisic acid-responsive HVA22 family
protein low similarity to SP|Q00765 Polyposis locus
protein 1 (TB2 protein) {Homo sapiens}; contains Pfam
profile PF03134: TB2/DP1, HVA22 family
Length = 227
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +2
Query: 332 VIGTFITEGDIGVGTIVGSAVFNILAVAACCGIGAGMVV 448
++G+ +T G++G+ + NI+ ACC IG G+ V
Sbjct: 13 LLGSGLT-GEVGLRVLFSPLSSNIVLRTACCSIGIGLPV 50
>At1g22550.1 68414.m02816 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 564
Score = 29.1 bits (62), Expect = 2.8
Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +2
Query: 455 DWWPLTRDCLAYGITVSILICIIHDEYVQWYEAFLLVSLYGVY-ICIMYYDKPIQNFAKG 631
+WW L+ L+ GIT+SI++ + + V W F + L+ V + + + + + +G
Sbjct: 194 NWWFLS---LSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYRYPRG 250
Query: 632 SWE 640
E
Sbjct: 251 DRE 253
>At4g21430.1 68417.m03097 transcription factor jumonji (jmjC)
domain-containing protein contains Pfam domain, PF02373:
jmjC domain
Length = 728
Score = 28.7 bits (61), Expect = 3.7
Identities = 18/64 (28%), Positives = 32/64 (50%)
Frame = -3
Query: 277 HIVCHVKRMTNFFNSRYKVFVANNCKS*KKIDGDERVQYDSSVLSLQLREESSREVIDSR 98
+ VC + + N+ +KV V N+ + KK+D SSVL L ++ S+E + +
Sbjct: 225 YAVCMLLPVLKEINAEHKVEVENDAE--KKVDLQRMCTRSSSVLRLNSDQDQSQESLSRK 282
Query: 97 RSAV 86
+V
Sbjct: 283 VGSV 286
>At5g56230.1 68418.m07017 prenylated rab acceptor (PRA1) family
protein contains Pfam profile PF03208: Prenylated rab
acceptor (PRA1)
Length = 186
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 458 WWPLTRDCLAYGITVSILICIIHDEYVQWYEAFL 559
W+ + LA G++V +L+CIIH + E FL
Sbjct: 137 WFTNSAVNLAVGVSVGLLLCIIHAVFRNSDELFL 170
>At1g55640.1 68414.m06369 prenylated rab acceptor (PRA1) family
protein weak similarity to prenylated Rab acceptor 1
(PRA1) [Homo sapiens] GI:4877285; contains Pfam profile
PF03208: Prenylated rab acceptor (PRA1)
Length = 187
Score = 28.3 bits (60), Expect = 4.9
Identities = 8/23 (34%), Positives = 15/23 (65%)
Frame = +2
Query: 458 WWPLTRDCLAYGITVSILICIIH 526
W+ + CL G+ S+L+C++H
Sbjct: 139 WFINSLQCLILGVVTSVLLCLVH 161
>At3g09170.1 68416.m01088 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 1000
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = -3
Query: 220 FVANNCKS*KKIDGDERVQYDSSVLSLQLREE 125
F+A++C + K DGD+ + D S ++ + REE
Sbjct: 359 FMADDCVNQAKGDGDDEMDVDGSGVAAEEREE 390
>At3g06140.1 68416.m00705 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 Zinc
finger, C3HC4 type (RING finger)
Length = 359
Score = 27.9 bits (59), Expect = 6.5
Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 3/31 (9%)
Frame = -2
Query: 434 HQYHNKPPQLIY*RQPN---PLWSPLRYHPP 351
+ YH +PPQ QPN P+ P Y PP
Sbjct: 72 YHYHPQPPQYFTTAQPNWWGPMMRPAYYCPP 102
>At2g13810.1 68415.m01524 aminotransferase class I and II family
protein low similarity to Aromatic Aminotransferase from
Pyrococcus horikoshii GP|14278621; contains Pfam profile
PF00155 aminotransferase, classes I and II
Length = 456
Score = 27.9 bits (59), Expect = 6.5
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -3
Query: 286 RSSHIVCHVKRMTNFFNSRYKVF 218
R HIV KR+ NFFN+R K F
Sbjct: 424 RRDHIVEASKRLQNFFNTRTKHF 446
>At1g34610.1 68414.m04301 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 997
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = -3
Query: 220 FVANNCKS*KKIDGDERVQYDSSVLSLQLREE 125
F+A++C + K DGD+ + D S ++ + REE
Sbjct: 359 FMADDCVNQAKGDGDDEMDVDGSGVAAEEREE 390
>At4g28690.1 68417.m04099 expressed protein
Length = 448
Score = 27.5 bits (58), Expect = 8.6
Identities = 14/41 (34%), Positives = 19/41 (46%), Gaps = 1/41 (2%)
Frame = -2
Query: 647 YPFPSSLSQNSV*ACHNTLCKCK-PRTMKLEEMLHTTEHTH 528
YPF S+L Q CH +C + P T + T+H H
Sbjct: 105 YPFKSTLHQTFCEMCHCYVCDTRAPCTYWFSGGISNTDHCH 145
>At4g08410.1 68417.m01390 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965;
Length = 707
Score = 27.5 bits (58), Expect = 8.6
Identities = 12/30 (40%), Positives = 15/30 (50%), Gaps = 1/30 (3%)
Frame = -2
Query: 455 PKEQPFPH-QYHNKPPQLIY*RQPNPLWSP 369
P P P Y + PPQ +Y P P +SP
Sbjct: 663 PYYSPSPKVDYKSSPPQYVYSSPPTPYYSP 692
>At2g30800.1 68415.m03755 DEIH-box RNA/DNA helicase, putative
similar to DEIH-box RNA/DNA helicase [Arabidopsis
thaliana] GI:5881579; contains Pfam profiles PF04408:
Helicase associated domain (HA2), PF00271: Helicase
conserved C-terminal domain
Length = 1299
Score = 27.5 bits (58), Expect = 8.6
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = +2
Query: 116 PAGLFTELQRQHGAIVLHALISIYLFLALAVVCDKYFVPAVEKICHA 256
P TEL + G + +H LIS LF A+ V C PA+ C A
Sbjct: 747 PQEELTELGEKFGHLPVHPLISKMLFFAVLVNC---LDPALTLACAA 790
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,282,468
Number of Sequences: 28952
Number of extensions: 320146
Number of successful extensions: 1146
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 866
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1145
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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