Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0296
(746 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 411 e-116
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 208 6e-55
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 205 5e-54
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 133 2e-32
SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit... 28 1.6
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 27 2.8
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 27 2.8
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 27 3.8
SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 27 3.8
SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 27 3.8
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.0
SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 26 5.0
SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 26 6.6
SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyce... 26 6.6
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 25 8.7
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 411 bits (1013), Expect = e-116
Identities = 186/246 (75%), Positives = 218/246 (88%)
Frame = +1
Query: 4 LSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQL 183
LS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y DLNHLVS M+GVTT RFPG+L
Sbjct: 187 LSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGEL 246
Query: 184 NADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPR 363
N+DLRKLAVNMVPFPRLHFFM GFAPL + GS ++A++VPELTQQMFDA NMM A DPR
Sbjct: 247 NSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPR 306
Query: 364 HGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAAT 543
HGRYLTVAA+FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+NV AVC +PP+ LKM+AT
Sbjct: 307 HGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSAT 366
Query: 544 FIGNSTAIQELFKRISEQFTAMFKRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 723
FIGNST+IQE+F+R+ +QF+AMF+RKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct: 367 FIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426
Query: 724 EATADE 741
EA DE
Sbjct: 427 EAGIDE 432
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 208 bits (508), Expect = 6e-55
Identities = 91/255 (35%), Positives = 152/255 (59%), Gaps = 8/255 (3%)
Frame = +1
Query: 4 LSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQL 183
L+ H ++N+D T+ +DNEA YDIC R L + PTY +LN L++ +S +T LRF G L
Sbjct: 193 LTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSL 252
Query: 184 NADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPR 363
N DL + N+VP+PR+HF + ++P+ S + + +V E+T Q F+ N M CDPR
Sbjct: 253 NVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPR 312
Query: 364 HGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPR------- 522
GRY+ ++RG + ++V + +I+++ + FV+W P K +C PP+
Sbjct: 313 TGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGI 372
Query: 523 -GLKMAATFIGNSTAIQELFKRISEQFTAMFKRKAFLHWYTGEGMDEMEFTEAESNMNDL 699
+ A + N+T+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L
Sbjct: 373 AKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 432
Query: 700 VSEYQQYQEATADED 744
+Y++ + + D +
Sbjct: 433 ERDYEEVGQDSMDNE 447
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 205 bits (501), Expect = 5e-54
Identities = 91/255 (35%), Positives = 150/255 (58%), Gaps = 8/255 (3%)
Frame = +1
Query: 4 LSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQL 183
L+ H ++ D T+ +DNE+ YDIC R L + P+Y +LN L++ +S +T LRF G L
Sbjct: 189 LTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSL 248
Query: 184 NADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPR 363
N DL + N+VP+PR+HF + +AP+ S + + +V E+T Q F+ N M CDPR
Sbjct: 249 NVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPR 308
Query: 364 HGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLK---- 531
GRY+ ++RG + ++V + I+ K + FV+W P K +CD PP+ ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 532 ----MAATFIGNSTAIQELFKRISEQFTAMFKRKAFLHWYTGEGMDEMEFTEAESNMNDL 699
A + N+T+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L
Sbjct: 369 AKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 428
Query: 700 VSEYQQYQEATADED 744
+Y++ + + + D
Sbjct: 429 ERDYEEVGQDSMEVD 443
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 133 bits (322), Expect = 2e-32
Identities = 73/251 (29%), Positives = 134/251 (53%), Gaps = 10/251 (3%)
Frame = +1
Query: 4 LSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQL 183
L++ +L N D +DN AL I L PT+ N LVS MS TT LR+PG +
Sbjct: 190 LALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYM 249
Query: 184 NADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL---TVPELTQQMFDAKNMMAAC 354
N DL + +++P PR HF + + P T++ ++ +A+ TV ++ +++ KN M +
Sbjct: 250 NNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSV 309
Query: 355 DP-RHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPP---R 522
+P + ++++ I +G +V + +L I+ + + F+ W P +++ A+ P
Sbjct: 310 NPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKT 369
Query: 523 GLKMAATFIGNSTAIQELFKRISEQFTAMFKRKAFLHWYTGEGMDEMEFTEAESNMN--- 693
+++ + N T+I LFKR +Q+ + KR AFL Y E + E + E +S+ +
Sbjct: 370 NHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVA 429
Query: 694 DLVSEYQQYQE 726
DL++EY+ ++
Sbjct: 430 DLINEYEACED 440
>SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit
Rep1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 472
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/59 (23%), Positives = 27/59 (45%)
Frame = -1
Query: 314 VSSGTVSARYCWLPRDVSGAKPGMKKCRRGKGTMLTASLRRSAFSCPGNRRHVVTPDMV 138
++ G + R C + A+ K+ +GK T++ + + P RRH+ D+V
Sbjct: 350 IAYGGIPMRVCHSDSTLCDARIAAKQALKGKRQYDTSTSKAELSTPPSKRRHIDDADLV 408
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 27.1 bits (57), Expect = 2.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +1
Query: 553 NSTAIQELFKRISEQFTAMFKRKAFLHWYTGEGMDE 660
NS+ IQ L K I+ T ++ ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/66 (21%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +1
Query: 391 IFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIG--NSTA 564
++ + ++ + + ++NI+ N S W+PN + +P G K++ I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 565 IQELFK 582
+EL++
Sbjct: 403 KEELYE 408
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 26.6 bits (56), Expect = 3.8
Identities = 22/68 (32%), Positives = 32/68 (47%)
Frame = +1
Query: 16 QLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 195
Q+ NTD + N LY RT+ DLN SL + GV +F G + +
Sbjct: 301 QVCTNTDSSRNAGNAILYQ-AVRTIL-------DLNSDSSLRVLGVNILAKFLGNRDNNT 352
Query: 196 RKLAVNMV 219
R +A+NM+
Sbjct: 353 RYVALNML 360
>SPBC56F2.07c |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 809
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -1
Query: 203 SLRRSAFSCPGNRRHVVTPDMVSDTRWFRSPYVGVDSFSVRKQI 72
+LR NR ++ P ++ R R YVG +F RKQI
Sbjct: 679 ALRNVLVLAATNRPDMIDPALMRPGRLDRLLYVGPPNFEARKQI 722
>SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 3.8
Identities = 14/53 (26%), Positives = 22/53 (41%)
Frame = -1
Query: 398 RKMAATVR*RPWRGSQAAIMFLASNICCVSSGTVSARYCWLPRDVSGAKPGMK 240
++MA+ R + A F A I CV + R W+ + G GM+
Sbjct: 496 KRMASLTNSREMQIQHAPTTFAAETIQCVLPIRIEKRVVWMEDGMEGGMEGMQ 548
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 5.0
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = +1
Query: 547 IGNSTAIQELFKRISEQFTAMFKRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 723
+ N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC
subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = -1
Query: 476 HSTKYEEFLFWMLSICSSTSFMDMRPRKMAATV 378
+ST +E+ + W++++ SST MRP + ATV
Sbjct: 199 NSTVFEDIMSWVVAMSSST----MRPIRHTATV 227
>SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit
Bgs4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1955
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/46 (30%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
Frame = -1
Query: 371 RPWRGSQAAIMFLASNICCVSSGTVS-ARYCWLPRDVSGAKPGMKK 237
+P SQ A + S + C+ S Y ++PR G+KP K+
Sbjct: 550 KPTGASQWAAVACTSVVSCIIMAAASLCEYLFVPRRFPGSKPIWKR 595
>SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +1
Query: 370 RYLTVAAIFRGRMSMKEVDEQMLNIQNKN--SSYFVEWIPNNVK 495
RYLT + + +++K V + +LN N++ + F+ W P K
Sbjct: 297 RYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSK 340
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/39 (28%), Positives = 17/39 (43%)
Frame = +1
Query: 370 RYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 486
+Y+ ++F G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,574,699
Number of Sequences: 5004
Number of extensions: 46162
Number of successful extensions: 209
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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