Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0284
(677 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A0C2I8 Cluster: Chromosome undetermined scaffold_144, w... 39 0.13
UniRef50_Q22LP0 Cluster: Putative uncharacterized protein; n=1; ... 35 2.1
UniRef50_A0CTJ7 Cluster: Chromosome undetermined scaffold_27, wh... 35 2.1
UniRef50_Q6LFH6 Cluster: Putative uncharacterized protein; n=1; ... 34 2.8
UniRef50_Q23TC4 Cluster: Putative uncharacterized protein; n=1; ... 34 2.8
UniRef50_A5UNZ2 Cluster: Magnesium chelatase subunit; n=1; Metha... 34 3.7
UniRef50_A4VEI2 Cluster: Putative uncharacterized protein; n=1; ... 33 4.8
UniRef50_A4AMG3 Cluster: Putative uncharacterized protein; n=1; ... 33 6.4
UniRef50_UPI000049A04F Cluster: hypothetical protein 276.t00007;... 33 8.4
UniRef50_UPI000069EC57 Cluster: Leukemia inhibitory factor precu... 33 8.4
UniRef50_Q74IH9 Cluster: Putative uncharacterized protein; n=2; ... 33 8.4
>UniRef50_A0C2I8 Cluster: Chromosome undetermined scaffold_144,
whole genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_144,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 838
Score = 38.7 bits (86), Expect = 0.13
Identities = 21/51 (41%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
Frame = +3
Query: 183 RRCAIKKIVQLLLSISKYNKITFLITFLLRQFDI-SFKVINAYGVSLGYIF 332
++C KI+ ++YNK++ L T+ LR FD+ SFK+I A+ S YIF
Sbjct: 426 QKCQYDKILMGAQQFNQYNKLSILFTYQLRIFDLDSFKIIFAF--SSSYIF 474
>UniRef50_Q22LP0 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 1125
Score = 34.7 bits (76), Expect = 2.1
Identities = 23/54 (42%), Positives = 30/54 (55%), Gaps = 9/54 (16%)
Frame = +3
Query: 366 SLHTSELI-----FSDLNIKSPKKNTSCKV----IKGLQKIFLILHYFQISYSL 500
SL+T LI F D++ KS NT C ++ + KIF+I YFQ SYSL
Sbjct: 684 SLNTQTLIIKKSNFIDISCKSTNNNTQCLGGSLNLQQINKIFIIADYFQNSYSL 737
>UniRef50_A0CTJ7 Cluster: Chromosome undetermined scaffold_27, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_27,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 323
Score = 34.7 bits (76), Expect = 2.1
Identities = 22/82 (26%), Positives = 32/82 (39%)
Frame = +3
Query: 180 QRRCAIKKIVQLLLSISKYNKITFLITFLLRQFDISFKVINAYGVSLGYIFCIDYHSVVK 359
Q RC+IK +++ S I F+ QFD K I L CI + +
Sbjct: 196 QIRCSIKTFLEVNTSFIIEQTIRFIRLEKENQFDQFIKGIQTIFQGLNPTACIKFEFKID 255
Query: 360 HLSLHTSELIFSDLNIKSPKKN 425
H H L+F N + + N
Sbjct: 256 HYFQHLINLVFQQFNFEEVQSN 277
>UniRef50_Q6LFH6 Cluster: Putative uncharacterized protein; n=1;
Plasmodium falciparum 3D7|Rep: Putative uncharacterized
protein - Plasmodium falciparum (isolate 3D7)
Length = 6077
Score = 34.3 bits (75), Expect = 2.8
Identities = 28/84 (33%), Positives = 40/84 (47%), Gaps = 1/84 (1%)
Frame = +3
Query: 213 LLLSISK-YNKITFLITFLLRQFDISFKVINAYGVSLGYIFCIDYHSVVKHLSLHTSELI 389
+L S+S K+T +I FLL I K + +L F D K+ T+EL
Sbjct: 5908 MLKSVSMAMKKLTNIIYFLLNNLTIKEKANDNKNQTLLNKFIYDKSGFKKNAHEITTELF 5967
Query: 390 FSDLNIKSPKKNTSCKVIKGLQKI 461
+D NI + KK TS + K L+ I
Sbjct: 5968 NTDYNIFNDKKYTSDNIKKFLENI 5991
>UniRef50_Q23TC4 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 966
Score = 34.3 bits (75), Expect = 2.8
Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 6/61 (9%)
Frame = +1
Query: 472 YIIFKSHTVFSIELIR*LKSGMWRYRPLFFRSVFKFAQ------KSSRLTMCGLTRNSAL 633
YIIF S+T+F I ++ K G +Y+ + VFKF + RL C LT +
Sbjct: 720 YIIFTSNTIFKISYLKLQKDGQDQYQHYIQKRVFKFVNYKHSRAERDRLISCCLTGQDTI 779
Query: 634 I 636
+
Sbjct: 780 L 780
>UniRef50_A5UNZ2 Cluster: Magnesium chelatase subunit; n=1;
Methanobrevibacter smithii ATCC 35061|Rep: Magnesium
chelatase subunit - Methanobrevibacter smithii (strain
PS / ATCC 35061 / DSM 861)
Length = 1671
Score = 33.9 bits (74), Expect = 3.7
Identities = 22/59 (37%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
Frame = -2
Query: 262 NVIRNVILLYFEILNSNCTIFLIAHRLCQTKICY-VKNSILN*RVKIFSYKILYESEFL 89
+V N++L Y + N +FL Q K + V+NS +N KIFS K +Y EFL
Sbjct: 156 SVFTNLLLKYPNLSNKELFLFLETSSSSQAKNLHLVRNSTIN-HEKIFSDKSIYTEEFL 213
>UniRef50_A4VEI2 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 223
Score = 33.5 bits (73), Expect = 4.8
Identities = 18/86 (20%), Positives = 43/86 (50%), Gaps = 3/86 (3%)
Frame = +3
Query: 237 NKITFLITFLLRQFDISFKVINAYGVSLGYIFCIDYHSVVKHLSLHTSEL--IFSDLNIK 410
NK+ F+ +L + ++ + +S+ + FC Y +K+ + +++ +FS +
Sbjct: 2 NKLDFIQNAILSLYTLTMQTNKKIQISISFHFCFSYF-FIKNADIQINQIMFLFSQTCHQ 60
Query: 411 SPK-KNTSCKVIKGLQKIFLILHYFQ 485
S N +CK + IF+ +++F+
Sbjct: 61 SQNFPNHNCKFFQTYSLIFICIYFFE 86
>UniRef50_A4AMG3 Cluster: Putative uncharacterized protein; n=1;
Flavobacteriales bacterium HTCC2170|Rep: Putative
uncharacterized protein - Flavobacteriales bacterium
HTCC2170
Length = 352
Score = 33.1 bits (72), Expect = 6.4
Identities = 16/40 (40%), Positives = 26/40 (65%)
Frame = +1
Query: 43 FIALIFIYSFHFI*FLKIHFRIKFYMKIFLLVNSK*SFSH 162
FI ++F+ S FI F+K++ RIK + F+L+ S +F H
Sbjct: 139 FIMIVFVMSPFFIRFIKLNIRIKLLIVFFMLICS--AFMH 176
>UniRef50_UPI000049A04F Cluster: hypothetical protein 276.t00007; n=1;
Entamoeba histolytica HM-1:IMSS|Rep: hypothetical protein
276.t00007 - Entamoeba histolytica HM-1:IMSS
Length = 975
Score = 32.7 bits (71), Expect = 8.4
Identities = 19/71 (26%), Positives = 35/71 (49%)
Frame = -2
Query: 430 DVFFFGDLMLRSEKINSLVCKDKCFTTL**SIQNIYPRDTPYAFMTLNEISNCLNKNVIR 251
++F DL I L+ K K T+ S+Q I +++ +F+ L + NK++I
Sbjct: 792 ELFVLYDLFKSDRTIEELLNKSKNLTSFYSSLQTIKSQESINSFLMLLPFQSFSNKSLID 851
Query: 250 NVILLYFEILN 218
+ Y ++LN
Sbjct: 852 TYLSYYIKLLN 862
>UniRef50_UPI000069EC57 Cluster: Leukemia inhibitory factor
precursor (LIF) (Differentiation- stimulating factor) (D
factor) (Melanoma-derived LPL inhibitor) (MLPLI)
(Emfilermin).; n=1; Xenopus tropicalis|Rep: Leukemia
inhibitory factor precursor (LIF) (Differentiation-
stimulating factor) (D factor) (Melanoma-derived LPL
inhibitor) (MLPLI) (Emfilermin). - Xenopus tropicalis
Length = 203
Score = 32.7 bits (71), Expect = 8.4
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +3
Query: 237 NKITFLITFLLRQFDISFKVINAYGVSLGYIFCIDYHSVVKHLSLHTSELIFSDLNIKSP 416
NKI+ T LL + + +F + +L C YH V H+S+H + S K P
Sbjct: 126 NKISSKKTPLLNELEKTFSGVKGILSNLSCFLCKKYH--VTHVSVHYGPPLPSG---KLP 180
Query: 417 KKNTSCKVIK 446
+K CKV+K
Sbjct: 181 QKAHGCKVLK 190
>UniRef50_Q74IH9 Cluster: Putative uncharacterized protein; n=2;
Lactobacillus|Rep: Putative uncharacterized protein -
Lactobacillus johnsonii
Length = 310
Score = 32.7 bits (71), Expect = 8.4
Identities = 19/69 (27%), Positives = 38/69 (55%)
Frame = +3
Query: 282 ISFKVINAYGVSLGYIFCIDYHSVVKHLSLHTSELIFSDLNIKSPKKNTSCKVIKGLQKI 461
+S K+IN + +S G++ + ++V + L T + ++ LN KN K + GL +
Sbjct: 182 LSDKIINHHQISRGFVLMYSHIAIVISVLLMTVDALY--LN----NKNVDRKFLYGLIVV 235
Query: 462 FLILHYFQI 488
++L+YF +
Sbjct: 236 TIVLYYFSL 244
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 591,134,462
Number of Sequences: 1657284
Number of extensions: 11436058
Number of successful extensions: 24384
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 23490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24378
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 52479343733
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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