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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0279
         (767 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g27600.1 68418.m03305 AMP-binding protein, putative similar t...    33   0.16 
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon...    28   6.0  

>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
           AMP-binding protein (MF39P) gi:1617274 from Brassica
           napus, long-chain-fatty-acid--CoA ligase - Brassica
           napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
           domain PF00501
          Length = 700

 Score = 33.5 bits (73), Expect = 0.16
 Identities = 17/41 (41%), Positives = 23/41 (56%)
 Frame = -2

Query: 151 KTDTAYSNCAFLSSN*FHGTSFNSKTVCAIFPVAPKYVKPY 29
           K +  Y+ C F+S    HG SFNS  V AI  V P+ +K +
Sbjct: 565 KIENVYTKCRFVSQCFIHGDSFNSSLV-AIVSVDPEVMKDW 604


>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
           long-chain acyl-CoA synthetase (LACS6) strong similarity
           to AMP-binding protein (MF39P) gi:1617274 from Brassica
           napus, similar to putative long-chain-fatty-acid--CoA
           ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
           contains Pfam AMP-binding enzyme domain PF00501;
           identical to cDNA AtLACS6 for long-chain acyl-CoA
           synthetase GI:22531705
          Length = 701

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = -2

Query: 151 KTDTAYSNCAFLSSN*FHGTSFNSKTVCAIFPVAPKYVKPY 29
           K +  Y+ C F+     +G SFNS  V A+  V P  +K +
Sbjct: 565 KIENVYAKCKFVGQCFIYGDSFNSSLV-AVVSVDPDVLKSW 604


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,128,153
Number of Sequences: 28952
Number of extensions: 289634
Number of successful extensions: 442
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 434
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 442
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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