BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0279 (767 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g27600.1 68418.m03305 AMP-binding protein, putative similar t... 33 0.16 At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon... 28 6.0 >At5g27600.1 68418.m03305 AMP-binding protein, putative similar to AMP-binding protein (MF39P) gi:1617274 from Brassica napus, long-chain-fatty-acid--CoA ligase - Brassica napus, EMBL:Z72152; contains Pfam AMP-binding enzyme domain PF00501 Length = 700 Score = 33.5 bits (73), Expect = 0.16 Identities = 17/41 (41%), Positives = 23/41 (56%) Frame = -2 Query: 151 KTDTAYSNCAFLSSN*FHGTSFNSKTVCAIFPVAPKYVKPY 29 K + Y+ C F+S HG SFNS V AI V P+ +K + Sbjct: 565 KIENVYTKCRFVSQCFIHGDSFNSSLV-AIVSVDPEVMKDW 604 >At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / long-chain acyl-CoA synthetase (LACS6) strong similarity to AMP-binding protein (MF39P) gi:1617274 from Brassica napus, similar to putative long-chain-fatty-acid--CoA ligase (brain isozyme) GB:P33124 [Rattus norvegicus]; contains Pfam AMP-binding enzyme domain PF00501; identical to cDNA AtLACS6 for long-chain acyl-CoA synthetase GI:22531705 Length = 701 Score = 28.3 bits (60), Expect = 6.0 Identities = 14/41 (34%), Positives = 21/41 (51%) Frame = -2 Query: 151 KTDTAYSNCAFLSSN*FHGTSFNSKTVCAIFPVAPKYVKPY 29 K + Y+ C F+ +G SFNS V A+ V P +K + Sbjct: 565 KIENVYAKCKFVGQCFIYGDSFNSSLV-AVVSVDPDVLKSW 604 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,128,153 Number of Sequences: 28952 Number of extensions: 289634 Number of successful extensions: 442 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 434 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 442 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1721869952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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