Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0278
(656 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 116 4e-27
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 111 1e-25
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 86 4e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 85 1e-17
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 60 4e-10
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 38 0.001
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 35 0.012
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 30 0.26
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 29 0.45
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 29 0.59
SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 29 0.78
SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|c... 28 1.4
SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual 27 2.4
SPCC24B10.12 |||CGI121 family protein|Schizosaccharomyces pombe|... 27 3.1
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 26 4.2
SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch... 26 5.5
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 25 9.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 116 bits (278), Expect = 4e-27
Identities = 56/138 (40%), Positives = 94/138 (68%), Gaps = 5/138 (3%)
Frame = +2
Query: 158 QIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEAP----GPINFTM 325
QIAEF+EAF++ D+++DG I ++L ++ SLG++PT L+ M+NE G I+FT
Sbjct: 10 QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTE 69
Query: 326 FLTLFGERLQGTDPEDVIKNAFGCFDEENNGVIGEERLRELLTTMGDRFTDDDVDEMLRE 505
FLT+ +++ TD E+ ++ AF FD++ NG I E L +LT++G+R + ++V +M+RE
Sbjct: 70 FLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIRE 129
Query: 506 API-RDGLFDYVEFTRIL 556
A DG+ +Y EF+R++
Sbjct: 130 ADTDGDGVINYEEFSRVI 147
Score = 36.7 bits (81), Expect = 0.003
Identities = 21/66 (31%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +2
Query: 143 MFDQAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEA----PGP 310
M D E +EAF + D++ +G+I ++L +L SLG+ +++ + M+ EA G
Sbjct: 78 MKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGV 137
Query: 311 INFTMF 328
IN+ F
Sbjct: 138 INYEEF 143
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 111 bits (266), Expect = 1e-25
Identities = 63/180 (35%), Positives = 100/180 (55%)
Frame = +2
Query: 41 GSKQLVFFTMSSRKTAGRRINKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFID 220
G+K+ F SS T+ +R+ + A+RA+S FA +QI E KEAF ++D++ DG I
Sbjct: 10 GAKRAPF---SSNTTSSQRVAAQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIG 66
Query: 221 KDDLHDMLASLGKNPTEDYLEGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCF 400
++D+ ML SL ++ +ED + M PIN FLT G L P + + AF F
Sbjct: 67 REDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRNDLLEAFSTF 126
Query: 401 DEENNGVIGEERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEFTRILKHGAKDKD 580
D+ +G I +R+ L++MGDR +V+ +LR + G+F Y +F + G+KD +
Sbjct: 127 DDTQSGKIPISTMRDALSSMGDRMDPQEVESILR-SYTSHGVFYYEKFVDAIA-GSKDSN 184
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 86.2 bits (204), Expect = 4e-18
Identities = 41/133 (30%), Positives = 83/133 (62%), Gaps = 4/133 (3%)
Frame = +2
Query: 170 FKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEAPGPINFTMFLTLF--- 340
+K+AF++ D++ G I K + D+L + G+NPT + + + P ++ FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVLNRP 67
Query: 341 -GERLQGTDPEDVIKNAFGCFDEENNGVIGEERLRELLTTMGDRFTDDDVDEMLREAPIR 517
G + G DPE+ +K F FD++ G+IG LR +LT++G++ +++++DE+L+ P++
Sbjct: 68 NGFDMPG-DPEEFVKG-FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 518 DGLFDYVEFTRIL 556
DG+ +Y +F +++
Sbjct: 126 DGMVNYHDFVQMI 138
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 84.6 bits (200), Expect = 1e-17
Identities = 44/132 (33%), Positives = 74/132 (56%), Gaps = 1/132 (0%)
Frame = +2
Query: 158 QIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEAPGPINFTMFLTL 337
Q E KEAF + D ++DG I + +L SLG N T+ L + NE I+ F++
Sbjct: 7 QTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSF 66
Query: 338 FGERLQGTDPEDVIKNAFGCFDEENNGVIGEERLRELLTTMGDRFTDDDVDEMLREA-PI 514
+L+ T+ E+ AF FD++N+G I + + + T+G++ +D++V M++EA P
Sbjct: 67 VSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPT 126
Query: 515 RDGLFDYVEFTR 550
G FDY +F +
Sbjct: 127 NSGSFDYYDFVQ 138
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 59.7 bits (138), Expect = 4e-10
Identities = 35/140 (25%), Positives = 67/140 (47%), Gaps = 5/140 (3%)
Frame = +2
Query: 152 QAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNE----APGPINF 319
+ Q + EAF + D ++D ID +L + +LG N + + ++ + G +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 320 TMFLTLFGERLQGTDPEDVIKNAFGCFDEENNGVIGEERLRELLTTMGDRFTDDDVDEML 499
F+ + E++ DP + IK AF FD++ G I LR + + + D +++ M+
Sbjct: 93 EDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMI 152
Query: 500 REAPI-RDGLFDYVEFTRIL 556
E + +DG + EF I+
Sbjct: 153 EEFDLDQDGEINEQEFIAIM 172
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/45 (40%), Positives = 29/45 (64%)
Frame = +2
Query: 167 EFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDYLEGMMNEA 301
E +EAF++ D G+ID +DL A LG+N T++ L+ M++ A
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLA 57
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 34.7 bits (76), Expect = 0.012
Identities = 28/118 (23%), Positives = 48/118 (40%), Gaps = 4/118 (3%)
Frame = +2
Query: 215 IDKDDLHDMLAS--LGKNPTEDYLEGMMNEAP-GPINFTMFLTLFGERLQGTDPEDVIKN 385
+ +D L D++ S K + + +G + P G +N + F ++ + DP +
Sbjct: 8 LSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEY 67
Query: 386 AFGCFDEENNGVIG-EERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEFTRIL 556
F FD + NG I +E + L T D + +GL Y E RI+
Sbjct: 68 VFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEMLRIV 125
Score = 29.1 bits (62), Expect = 0.59
Identities = 16/61 (26%), Positives = 28/61 (45%)
Frame = +2
Query: 98 INKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLGKNPTEDY 277
+NK Q+ F D + AE+ FN+ D +++G+ID + L+ + D
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEY--VFNVFDADKNGYIDFKEFICALSVTSRGELNDK 100
Query: 278 L 280
L
Sbjct: 101 L 101
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 30.3 bits (65), Expect = 0.26
Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = -3
Query: 420 TPLFSSSKHPNAFLMTSSGSVPCSLSPNRVRNIVKLIGPGASFIIPSK*SSV-GFLPKDA 244
TP S P +++SS S+P S S + +I + +S IPS SSV L
Sbjct: 525 TPSSSIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSSSIPSTFSSVSSILSSST 584
Query: 243 SISCRSSLSMNPS 205
S +SLS++ S
Sbjct: 585 SSPSSTSLSISSS 597
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 29.5 bits (63), Expect = 0.45
Identities = 15/45 (33%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = +2
Query: 167 EFKEAFNMIDQNRDGFIDKDDLHDMLASL-GKNPTEDYLEGMMNE 298
+ K AF + D +RDG+I +L+ +L + G N ED L+ ++++
Sbjct: 94 KLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDK 138
>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
Apc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1458
Score = 29.1 bits (62), Expect = 0.59
Identities = 12/43 (27%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = -2
Query: 202 VLINHIESLLELCYLSLIKHSKHIGGSTL--CALLVNATTGCL 80
+LIN ++S + LC+L H + + T+ C +V ++ C+
Sbjct: 1119 ILINFLDSFIRLCHLPAKTHDERVTAVTVIRCTQIVALSSSCV 1161
>SPCC1259.11c |gyp2||GTPase activating protein Gyp2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 720
Score = 28.7 bits (61), Expect = 0.78
Identities = 19/53 (35%), Positives = 25/53 (47%), Gaps = 5/53 (9%)
Frame = +2
Query: 305 GP-INFTMFLTLFGERLQG----TDPEDVIKNAFGCFDEENNGVIGEERLREL 448
GP + F + L LF QG TD V+K CFD N G +E++ L
Sbjct: 404 GPRVLFQVALALFKVNAQGILNATDDSSVMKVFRQCFDHINQGTAADEKMAAL 456
>SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 371
Score = 27.9 bits (59), Expect = 1.4
Identities = 14/56 (25%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = +2
Query: 149 DQAQIAEFKEAFNMIDQNRDGFIDKDDLHDML--ASLGKNPTEDYLEGMMNEAPGP 310
+ ++ EF+E N + + +DK D HD + A + K P ++ + A P
Sbjct: 267 ESPRVEEFEELLNQFEGDEKVSVDKIDAHDKMTEAQVVKIPPVQFMNARVAAAENP 322
>SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 27.1 bits (57), Expect = 2.4
Identities = 12/44 (27%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Frame = -2
Query: 508 CFPEHFIHIIVCEAIAHCGKQL-AESLLTNHTIVLFIEAPKCIF 380
CF F H I C A+ K + + +T +T++ + +P I+
Sbjct: 137 CFASIFFHAIACYALYLLTKSIFSNQKMTAYTVIFYCFSPSGIY 180
>SPCC24B10.12 |||CGI121 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 174
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/65 (21%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = -2
Query: 505 FPEHFIHIIVCEAIAHCGKQLAESLLTNHTI--VLFIEAPKCIFDDILRVSALQSLTKQS 332
FPE +H+ V E +++C + E L++ + I F++A ++ + + +++L +
Sbjct: 6 FPETQVHVFVYENVSNCA-AIHEQLISQNPIYDYAFLDAATILYKKQVYSAIIRALEDRR 64
Query: 331 EKHRK 317
++ K
Sbjct: 65 DEQMK 69
>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1155
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +2
Query: 359 TDPEDVIKNAFGCFDEENNGVIGEERLRELLTTMGDRFTDDDVDEM 496
TD D++KN F F+ +N + L +L + DD + +
Sbjct: 826 TDESDIVKNTFVSFNTTSNSLGNTTALSQLKGHINSVIVDDSYNNI 871
>SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 681
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -2
Query: 397 APKCIFDDILRVSALQSLTKQSEKHRKVNWS 305
A + + D+I VSA+QS + S +V WS
Sbjct: 590 ATETVLDEIQGVSAVQSEAESSAAEMRVYWS 620
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 9.6
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +1
Query: 436 TPRAAYHNGRSLHRR 480
+PR A+HNG LH++
Sbjct: 23 SPRCAFHNGGILHKK 37
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,693,241
Number of Sequences: 5004
Number of extensions: 55230
Number of successful extensions: 167
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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