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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0275
         (687 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g25520.2 68418.m03037 transcription elongation factor-related...    46   2e-05
At5g25520.1 68418.m03036 transcription elongation factor-related...    46   2e-05
At5g05140.1 68418.m00545 transcription elongation factor-related...    46   2e-05
At5g11430.1 68418.m01335 transcription elongation factor-related...    46   2e-05
At2g25640.1 68415.m03073 transcription elongation factor-related...    44   9e-05
At3g10820.1 68416.m01303 transcription elongation factor-related...    42   3e-04
At2g38560.1 68415.m04737 transcription factor S-II (TFIIS) domai...    40   0.001
At4g36180.1 68417.m05148 leucine-rich repeat family protein cont...    27   8.8  

>At5g25520.2 68418.m03037 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 997

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 25/71 (35%), Positives = 34/71 (47%)
 Frame = +1

Query: 451 ETSNACGSPXXXXXXXXXCIYAEFKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNGVIS 630
           ET  +   P          ++  F   + +YK + RS + NLKD  NP LR +  +G IS
Sbjct: 352 ETQKSFEDPELLASKIELELFKLFGGVNKKYKEKGRSLLFNLKDKNNPELRESVMSGKIS 411

Query: 631 ASRLAKMTPEE 663
             RL  MT EE
Sbjct: 412 PERLCNMTAEE 422


>At5g25520.1 68418.m03036 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 735

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 25/71 (35%), Positives = 34/71 (47%)
 Frame = +1

Query: 451 ETSNACGSPXXXXXXXXXCIYAEFKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNGVIS 630
           ET  +   P          ++  F   + +YK + RS + NLKD  NP LR +  +G IS
Sbjct: 352 ETQKSFEDPELLASKIELELFKLFGGVNKKYKEKGRSLLFNLKDKNNPELRESVMSGKIS 411

Query: 631 ASRLAKMTPEE 663
             RL  MT EE
Sbjct: 412 PERLCNMTAEE 422


>At5g05140.1 68418.m00545 transcription elongation factor-related
           low similarity to transcription elongation factor
           TFIIS.h [Mus musculus] GI:3288547, elongin A [Mus
           musculus] GI:6009624
          Length = 436

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 24/51 (47%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
 Frame = +1

Query: 133 MAINLDVLTKTRIGMTVNALRKSSKDDEVISLCKTLIKNWKKFLST-PNTT 282
           M++N+D+L  T IG  VN LRK S  D++  L KTLI  WK+ +    NTT
Sbjct: 169 MSLNVDILKSTEIGKAVNGLRKHS-SDKIRQLAKTLIAEWKELVDQWVNTT 218


>At5g11430.1 68418.m01335 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 871

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 23/52 (44%), Positives = 29/52 (55%)
 Frame = +1

Query: 508 IYAEFKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNGVISASRLAKMTPEE 663
           +Y  F   + +Y+ R RS + NLKD  NP LR    +  ISA RL  MT EE
Sbjct: 270 LYKLFGGVNKKYRERGRSLLFNLKDKNNPELRERVMSEEISAERLCSMTAEE 321


>At2g25640.1 68415.m03073 transcription elongation factor-related
           contains weak similarity to transcription elongation
           factors
          Length = 643

 Score = 44.0 bits (99), Expect = 9e-05
 Identities = 25/86 (29%), Positives = 37/86 (43%)
 Frame = +1

Query: 406 LKCRELLTQALKAAGETSNACGSPXXXXXXXXXCIYAEFKNTDMRYKNRVRSRVANLKDP 585
           + C+  +  A+   G        P          ++  F   + +YK + RS + NLKD 
Sbjct: 242 VNCQNEMIGAMANDGSLEKLLLDPQVLAFEIETELFKLFGGVNKKYKEKGRSLLFNLKDK 301

Query: 586 KNPTLRTNFFNGVISASRLAKMTPEE 663
            NP LR     G I+A RL  M+ EE
Sbjct: 302 SNPKLREKVMYGEIAAERLCSMSAEE 327


>At3g10820.1 68416.m01303 transcription elongation factor-related
           low similarity to transcription elongation factor
           TFIIS.h [Mus musculus] GI:3288547
          Length = 416

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 26/81 (32%), Positives = 45/81 (55%)
 Frame = +1

Query: 22  MSVEEDVMKIQKKLTKMTSEDGTGQXXXXXXXXXXQTMAINLDVLTKTRIGMTVNALRKS 201
           +SV+E VM+I+  L+   ++D              ++M++++D+L  T IG  VN LR+ 
Sbjct: 105 VSVDE-VMRIRDILS---NKDDEKDSVLLESLRKLESMSMSVDILKDTEIGKAVNGLRRH 160

Query: 202 SKDDEVISLCKTLIKNWKKFL 264
           S D ++  L KTL   WK+ +
Sbjct: 161 SSD-KISKLAKTLFAEWKRLV 180


>At2g38560.1 68415.m04737 transcription factor S-II (TFIIS)
           domain-containing protein similar to SP|P49373
           Transcription elongation factor S-II (TFIIS)
           {Schizosaccharomyces pombe}; contains Pfam profile
           PF01096: Transcription factor S-II (TFIIS)
          Length = 378

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 32/116 (27%), Positives = 47/116 (40%), Gaps = 7/116 (6%)
 Frame = +1

Query: 361 PASFPPQSNTTDAVRLKCRELLTQAL-KAAGETSNA------CGSPXXXXXXXXXCIYAE 519
           P          D VR K RELL +AL + AGE  +          P          ++ +
Sbjct: 197 PPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYERESVNASDPLRVAVSVESLMFEK 256

Query: 520 FKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNGVISASRLAKMTPEEWQVMKWKK 687
              +    K + RS + NL+D  NP LR     G IS  +L  ++ E+    K K+
Sbjct: 257 LGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISPEKLITLSAEDMASDKRKQ 312


>At4g36180.1 68417.m05148 leucine-rich repeat family protein
           contains protein kinase domain, Pfam:PF00069; contains
           leucine-rich repeats, Pfam:PF00560
          Length = 1136

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 12/40 (30%), Positives = 22/40 (55%)
 Frame = +1

Query: 517 EFKNTDMRYKNRVRSRVANLKDPKNPTLRTNFFNGVISAS 636
           E +   ++   R+  R++ L+  +  +LR+N FNG I  S
Sbjct: 72  EIRLPRLQLSGRISDRISGLRMLRKLSLRSNSFNGTIPTS 111


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.314    0.127    0.364 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,190,552
Number of Sequences: 28952
Number of extensions: 174650
Number of successful extensions: 477
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 462
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 476
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)

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