BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0272 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1E7.10 |rpc17||DNA-directed RNA polymerase III complex subu... 51 2e-07 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 29 0.84 SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 28 1.1 SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 28 1.5 SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 27 2.6 SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe... 27 3.4 SPAC12G12.02 ||SPAC630.01c|rRNA processing protein, unnamed|Schi... 27 3.4 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 26 4.5 SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 26 5.9 SPAPB24D3.09c |pdr1||ABC transporter Pdr1|Schizosaccharomyces po... 25 7.9 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 7.9 SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 25 7.9 >SPAPB1E7.10 |rpc17||DNA-directed RNA polymerase III complex subunit Rpc17|Schizosaccharomyces pombe|chr 1|||Manual Length = 129 Score = 50.8 bits (116), Expect = 2e-07 Identities = 30/125 (24%), Positives = 66/125 (52%), Gaps = 9/125 (7%) Frame = +3 Query: 99 METIKANSAFLCNYEVMQILQKLKDN----THKKHKREA----SLATVTYETVHYLQDT- 251 M+ ++A A+L N EV L+++++ T ++ +A +L T+ +E + YL Sbjct: 1 MKVLEARDAYLTNAEVFFHLKEMENEQNARTQERGAAQALVCENLRTIQFEILKYLSSQG 60 Query: 252 ECKNQSAQKIQKFLEAMKKYKLTKVEKLMMVNTPPRTELEIQLIVQESEERLSEEDVQDI 431 C+ + ++ + +++LTK E L+++N P + E+ ++ EER EED+ + Sbjct: 61 NCEGLTKERFLDCIAIFNEFELTKAEILVILNNKPSSVPELYACIEGIEERFKEEDIFKL 120 Query: 432 ISIVS 446 + ++ Sbjct: 121 VEKIN 125 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 28.7 bits (61), Expect = 0.84 Identities = 14/50 (28%), Positives = 25/50 (50%) Frame = +3 Query: 303 KKYKLTKVEKLMMVNTPPRTELEIQLIVQESEERLSEEDVQDIISIVSQH 452 ++ K T+V L +VN PP+T + + + E L E + S+V + Sbjct: 487 RRTKETRVNNLPIVNLPPKTIRTVSVNLLPEERALYNEQMSSAQSLVDNY 536 >SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 708 Score = 28.3 bits (60), Expect = 1.1 Identities = 13/40 (32%), Positives = 23/40 (57%) Frame = +3 Query: 294 EAMKKYKLTKVEKLMMVNTPPRTELEIQLIVQESEERLSE 413 E +KKY TKVE++ PP + L ++ E++ +S+ Sbjct: 170 EFLKKYSSTKVERVSEA-APPPSSLNSSTVLDENDSLISQ 208 >SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Spt6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1365 Score = 27.9 bits (59), Expect = 1.5 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = -1 Query: 270 HSDFCILCLVSNVQSHM*QWQVKLLSYAFYV 178 H + ILC ++V S + W VK++ AF++ Sbjct: 1306 HPGYFILCFKASVNSPVTAWPVKVIPNAFFL 1336 >SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual Length = 539 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +3 Query: 570 KIAYFVRTRNLKHKQLAGVFWRPI 641 K +F+RT N KH+ FW+ I Sbjct: 105 KFTHFIRTTNPKHQASVQEFWKTI 128 >SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 26.6 bits (56), Expect = 3.4 Identities = 16/46 (34%), Positives = 25/46 (54%) Frame = -3 Query: 382 INCISNSVLGGVFTIINFSTLVSLYFFIASRNF*IFWAL*FLHSVS 245 + I N+ L V+ I S LVSL++F + NF F+++VS Sbjct: 98 LQLIKNTSLISVYIGIVISLLVSLFYFYSLPNFPYSKTCIFIYTVS 143 >SPAC12G12.02 ||SPAC630.01c|rRNA processing protein, unnamed|Schizosaccharomyces pombe|chr 1|||Manual Length = 183 Score = 26.6 bits (56), Expect = 3.4 Identities = 26/86 (30%), Positives = 39/86 (45%), Gaps = 1/86 (1%) Frame = +3 Query: 225 ETVHYLQDTECKNQSAQKIQKFLEAMKKYK-LTKVEKLMMVNTPPRTELEIQLIVQESEE 401 ET+ + +C+ +S +K Q L +K+Y LTK L T +TE I E EE Sbjct: 88 ETMDDEKRKQCE-KSMRKCQIDLMYIKEYPPLTKYVSLYAEGTSEQTEETRNRIWAEMEE 146 Query: 402 RLSEEDVQDIISIVSQHLLANDSSNT 479 R + I S S + + S+T Sbjct: 147 RFNSGRKHKIPSSGSNRVPVQEKSST 172 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 26.2 bits (55), Expect = 4.5 Identities = 15/62 (24%), Positives = 31/62 (50%) Frame = +3 Query: 132 CNYEVMQILQKLKDNTHKKHKREASLATVTYETVHYLQDTECKNQSAQKIQKFLEAMKKY 311 CN+ +L KL ++T KR+ + + + + H+ + +N+ A ++ L MK Sbjct: 412 CNHAYNTMLCKLTEDTSSFLKRQTEMLSHSPASSHWASFSSLENEIA-NLKNLLSKMKST 470 Query: 312 KL 317 +L Sbjct: 471 EL 472 >SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 1854 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/27 (40%), Positives = 18/27 (66%), Gaps = 1/27 (3%) Frame = -1 Query: 684 YRKSFLR-YMVHAMYLLVSRKPLPVAY 607 +R++F Y+VH ++LL P P+AY Sbjct: 1270 WRRTFTGLYIVHLLFLLTIFYPCPIAY 1296 >SPAPB24D3.09c |pdr1||ABC transporter Pdr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1396 Score = 25.4 bits (53), Expect = 7.9 Identities = 8/26 (30%), Positives = 19/26 (73%) Frame = +3 Query: 243 QDTECKNQSAQKIQKFLEAMKKYKLT 320 ++T+C ++ QK++++LE + + LT Sbjct: 17 KNTKCPDKFEQKVEEYLEVLNELHLT 42 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = -1 Query: 252 LCLVSNVQSHM*QWQVKLLSYAFYVCYLLTF 160 LC ++ + SH+ WQ K+L YV L+ F Sbjct: 693 LCNITFLGSHLCIWQPKILLGIMYVTDLVLF 723 >SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 872 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -1 Query: 573 SLCNTYHYLKEYHFT*VYSTID 508 SL N Y+Y ++ H T VYS D Sbjct: 680 SLLNDYNYNRQSHITRVYSGTD 701 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,979,744 Number of Sequences: 5004 Number of extensions: 61828 Number of successful extensions: 173 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 173 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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