Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0270
(672 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A4HBW0 Cluster: Putative uncharacterized protein; n=1; ... 35 1.6
UniRef50_A7CPF8 Cluster: Putative uncharacterized protein; n=1; ... 34 2.7
UniRef50_Q7QS00 Cluster: GLP_228_26673_25900; n=1; Giardia lambl... 34 2.7
UniRef50_UPI00006CB12C Cluster: Protein kinase domain containing... 34 3.6
UniRef50_UPI0000ECAA36 Cluster: ADP-ribosylation factor-binding ... 34 3.6
UniRef50_A2DM41 Cluster: Putative uncharacterized protein; n=1; ... 34 3.6
UniRef50_Q39AF4 Cluster: Outer membrane protein, Haemagluttinin-... 33 4.8
UniRef50_Q7QST2 Cluster: GLP_127_30424_27701; n=1; Giardia lambl... 33 4.8
UniRef50_A2F336 Cluster: Chitinase, putative; n=2; Trichomonas v... 33 4.8
UniRef50_Q5V4R3 Cluster: Serine/threonine protien kinase related... 33 4.8
UniRef50_UPI0000F1FBE5 Cluster: PREDICTED: similar to GH09355p; ... 33 6.3
UniRef50_Q88YB0 Cluster: Extracellular protein; n=1; Lactobacill... 33 6.3
UniRef50_Q54SV4 Cluster: Putative uncharacterized protein; n=1; ... 33 6.3
UniRef50_Q6C1G3 Cluster: Similarities with DEHA0E11308g Debaryom... 33 6.3
UniRef50_Q03NT1 Cluster: Putative uncharacterized protein; n=1; ... 33 8.3
UniRef50_Q09624 Cluster: Uncharacterized protein ZK945.9; n=3; r... 33 8.3
UniRef50_Q13620 Cluster: Cullin-4B; n=95; Eukaryota|Rep: Cullin-... 33 8.3
>UniRef50_A4HBW0 Cluster: Putative uncharacterized protein; n=1;
Leishmania braziliensis|Rep: Putative uncharacterized
protein - Leishmania braziliensis
Length = 2085
Score = 35.1 bits (77), Expect = 1.6
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +1
Query: 145 RQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQAQSRLTADFEQHWRQSRSIQA 324
R + S P Q QQ S+L+ P P ++++ +++A + LTA + S S QA
Sbjct: 1471 RDAQSSAPTGQQQQREASQLSPPPPPPPLPHSSAGTAVEAPAELTAAAQSTTYSSPSRQA 1530
Query: 325 Q 327
Q
Sbjct: 1531 Q 1531
>UniRef50_A7CPF8 Cluster: Putative uncharacterized protein; n=1;
Opitutaceae bacterium TAV2|Rep: Putative uncharacterized
protein - Opitutaceae bacterium TAV2
Length = 413
Score = 34.3 bits (75), Expect = 2.7
Identities = 23/59 (38%), Positives = 29/59 (49%)
Frame = -1
Query: 429 SPPRLEVVSYLVIVVRAEISLQVPVLLEVCGESKLSLNAA*LSPVLLEVCGESRLSLNA 253
+P EV Y + VRA + PVL EV G + NA +PVL EV G + NA
Sbjct: 207 APVLAEVSGY--VDVRANATFTAPVLAEVSGYVDVRANATFTAPVLAEVSGYVDVRANA 263
Score = 33.1 bits (72), Expect = 6.3
Identities = 21/51 (41%), Positives = 26/51 (50%)
Frame = -1
Query: 429 SPPRLEVVSYLVIVVRAEISLQVPVLLEVCGESKLSLNAA*LSPVLLEVCG 277
+P EV Y + VRA + PVL EV G + NA +PVL EV G
Sbjct: 227 APVLAEVSGY--VDVRANATFTAPVLAEVSGYVDVRANATFTAPVLAEVSG 275
>UniRef50_Q7QS00 Cluster: GLP_228_26673_25900; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_228_26673_25900 - Giardia lamblia
ATCC 50803
Length = 257
Score = 34.3 bits (75), Expect = 2.7
Identities = 26/97 (26%), Positives = 46/97 (47%)
Frame = +2
Query: 80 SSAHQELRLPQSPHSRR*K*VVDSRPTASQHRSLNRTIAADSPRFLTLRHAPTLPHRAAF 259
SS+HQ PQSP S S ++S S + + ++ S + + + ++
Sbjct: 90 SSSHQPTPRPQSPSSSS-----SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 144
Query: 260 KLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCK 370
+ S S + S+T ++ LS SP+TSSS+ + K
Sbjct: 145 RSSSHSSSSHSTTDSEESSGSLSVPSPETSSSSSSSK 181
>UniRef50_UPI00006CB12C Cluster: Protein kinase domain containing
protein; n=1; Tetrahymena thermophila SB210|Rep: Protein
kinase domain containing protein - Tetrahymena
thermophila SB210
Length = 1318
Score = 33.9 bits (74), Expect = 3.6
Identities = 22/79 (27%), Positives = 39/79 (49%), Gaps = 1/79 (1%)
Frame = +1
Query: 124 SQIKVSRRQS-SHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQAQSRLTADFEQHW 300
S++ S Q+ S+ + + Q D + T D +++ IQAQS+ + +F+Q W
Sbjct: 699 SEVSSSINQTNSNIEKVTHVQFDPNTNSTQNLDTEVYVQAQTTQEIQAQSQ-SQNFQQWW 757
Query: 301 RQSRSIQAQFRLTANFEQH 357
+ R+I QF A +H
Sbjct: 758 TEKRNINLQFISCAKLNKH 776
>UniRef50_UPI0000ECAA36 Cluster: ADP-ribosylation factor-binding
protein GGA2 (Golgi-localized, gamma ear-containing,
ARF-binding protein 2) (Gamma-adaptin-related protein 2)
(VHS domain and ear domain of gamma-adaptin) (Vear).;
n=3; Amniota|Rep: ADP-ribosylation factor-binding
protein GGA2 (Golgi-localized, gamma ear-containing,
ARF-binding protein 2) (Gamma-adaptin-related protein 2)
(VHS domain and ear domain of gamma-adaptin) (Vear). -
Gallus gallus
Length = 610
Score = 33.9 bits (74), Expect = 3.6
Identities = 25/85 (29%), Positives = 39/85 (45%)
Frame = +1
Query: 79 IISASRTSATTIPAFSQIKVSRRQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSI 258
I SASR S+TT + ++ S S QP D L+ + DP A + S+++
Sbjct: 329 ITSASRHSSTTSACLLEEELKSLDLSDSLVLQQPPPDF--ALSEVSDPQAAVHNGYSKNV 386
Query: 259 QAQSRLTADFEQHWRQSRSIQAQFR 333
A L + E+ W S + +FR
Sbjct: 387 SAFEPL--ELEESWEDSCLEKNEFR 409
>UniRef50_A2DM41 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 934
Score = 33.9 bits (74), Expect = 3.6
Identities = 21/60 (35%), Positives = 34/60 (56%)
Frame = +2
Query: 239 LPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKYDTTSS 418
LPH + S ++ +SSST + S +S TSSS+ T + ++ +T T+ +TTSS
Sbjct: 106 LPHHQPLQRS-ETTSSSSSTESETTSSSSSTESETTSSSSSTERETTSSSTSTESETTSS 164
>UniRef50_Q39AF4 Cluster: Outer membrane protein, Haemagluttinin-like;
n=27; Burkholderia|Rep: Outer membrane protein,
Haemagluttinin-like - Burkholderia sp. (strain 383)
(Burkholderia cepacia (strain ATCC 17760/ NCIB 9086 /
R18194))
Length = 2866
Score = 33.5 bits (73), Expect = 4.8
Identities = 24/53 (45%), Positives = 32/53 (60%)
Frame = +2
Query: 266 SLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKYDTTSSLG 424
S+DS TS+STG + A S S TS+STG L SA ++IT T++S G
Sbjct: 1669 SIDSLSTSTSTGLSSA--NSSIGSLSTSTSTG---LSSANSSITSLSTSTSTG 1716
>UniRef50_Q7QST2 Cluster: GLP_127_30424_27701; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_127_30424_27701 - Giardia lamblia
ATCC 50803
Length = 907
Score = 33.5 bits (73), Expect = 4.8
Identities = 27/81 (33%), Positives = 43/81 (53%)
Frame = +1
Query: 82 ISASRTSATTIPAFSQIKVSRRQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQ 261
++A+ TT+ S I S SHS P + RS L+A D T+ ++SS +I+
Sbjct: 175 VNAALDDTTTLSDHSFIGRSSSNISHSNPFRSHSEQARSMLSA-SDLTSSVGSSSSGTIR 233
Query: 262 AQSRLTADFEQHWRQSRSIQA 324
+ S+ TAD RQ++ I+A
Sbjct: 234 STSK-TADMS---RQNKDIRA 250
>UniRef50_A2F336 Cluster: Chitinase, putative; n=2; Trichomonas
vaginalis G3|Rep: Chitinase, putative - Trichomonas
vaginalis G3
Length = 739
Score = 33.5 bits (73), Expect = 4.8
Identities = 31/127 (24%), Positives = 58/127 (45%)
Frame = +2
Query: 50 ATEQSQPSD*SSAHQELRLPQSPHSRR*K*VVDSRPTASQHRSLNRTIAADSPRFLTLRH 229
++ +S+ + SS+ E S S + S T S+ S + + +++ T
Sbjct: 327 SSTESETTSSSSSSTESETTSSSSSTESETTSSSSSTESETTSSSSSTESET----TSSS 382
Query: 230 APTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKYDT 409
+ T + S +S TSSS+ ++ S +S TSSS+ T ++ ++ T+ +T
Sbjct: 383 SSTESETTSSSSSTESETTSSSSTESETTSSSSTESETTSSSSSTESETTSSSSSTESET 442
Query: 410 TSSLGGE 430
TSS E
Sbjct: 443 TSSSSTE 449
>UniRef50_Q5V4R3 Cluster: Serine/threonine protien kinase related
protein; n=1; Haloarcula marismortui|Rep:
Serine/threonine protien kinase related protein -
Haloarcula marismortui (Halobacterium marismortui)
Length = 427
Score = 33.5 bits (73), Expect = 4.8
Identities = 27/90 (30%), Positives = 35/90 (38%), Gaps = 2/90 (2%)
Frame = +1
Query: 82 ISASRTSATTIPAFSQIKVSRRQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQ 261
I S T P + +QS ++P QQ S +TA P T + S
Sbjct: 30 IETSPIGTETTPELEAGAAAHKQSRETEPFVAWQQSLGSPITAPPISTGSGLYVGTESGT 89
Query: 262 AQSRLTADFEQHWRQSRS--IQAQFRLTAN 345
+S TAD Q W S I+AQ R N
Sbjct: 90 VKSFATADGSQQWMYDASDPIRAQPRCVGN 119
>UniRef50_UPI0000F1FBE5 Cluster: PREDICTED: similar to GH09355p;
n=1; Danio rerio|Rep: PREDICTED: similar to GH09355p -
Danio rerio
Length = 654
Score = 33.1 bits (72), Expect = 6.3
Identities = 20/65 (30%), Positives = 37/65 (56%)
Frame = +1
Query: 82 ISASRTSATTIPAFSQIKVSRRQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQ 261
+ ++ TSA+ +P + K+ + SS S+P+ QP+QDH P + + T S+R+
Sbjct: 575 VFSAPTSASKLPV--KPKIQLKSSSASQPSGQPKQDHEE-----PSVGSLSKTPSNRATY 627
Query: 262 AQSRL 276
+S+L
Sbjct: 628 FRSKL 632
>UniRef50_Q88YB0 Cluster: Extracellular protein; n=1; Lactobacillus
plantarum|Rep: Extracellular protein - Lactobacillus
plantarum
Length = 172
Score = 33.1 bits (72), Expect = 6.3
Identities = 20/55 (36%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = +2
Query: 275 SPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKY---DTTSSLGGE 430
S T+SST DN+++ + D ++S++ + S+ TT T DTT+S GGE
Sbjct: 118 SSATNSSTTDNNSSTTDTSDDDESSAAESSSTTASSSTTTTSQSTTDTTTSDGGE 172
>UniRef50_Q54SV4 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 950
Score = 33.1 bits (72), Expect = 6.3
Identities = 19/60 (31%), Positives = 27/60 (45%)
Frame = +1
Query: 139 SRRQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQAQSRLTADFEQHWRQSRSI 318
S SS S S PQQ H + + T+S +IQ S+ D E +++S SI
Sbjct: 728 SSSSSSSSPSPSSPQQQHDFNINNKKEQIQLLLTSSLENIQLMSKNVKDIENQFKESCSI 787
>UniRef50_Q6C1G3 Cluster: Similarities with DEHA0E11308g
Debaryomyces hansenii IPF 12024.1; n=1; Yarrowia
lipolytica|Rep: Similarities with DEHA0E11308g
Debaryomyces hansenii IPF 12024.1 - Yarrowia lipolytica
(Candida lipolytica)
Length = 531
Score = 33.1 bits (72), Expect = 6.3
Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 1/82 (1%)
Frame = +3
Query: 99 FGYHNPRILADKSK-SSTVVPQQASIAASTGPSQPTHRAS*PYGMHLHCLIAQHSSSVST 275
FGY + K + S QQ +A +T P+Q +H S + H H + HS SV
Sbjct: 174 FGYLQMHFNSTKKRRKSNAKAQQQGLAHATSPTQHSHPTSPSHSPHSHMI---HSQSVPH 230
Query: 276 HRRLRAALATITQHSSSVSTHR 341
+ +L HS H+
Sbjct: 231 MAQHHMSLQQHVAHSQQQQQHQ 252
>UniRef50_Q03NT1 Cluster: Putative uncharacterized protein; n=1;
Lactobacillus brevis ATCC 367|Rep: Putative
uncharacterized protein - Lactobacillus brevis (strain
ATCC 367 / JCM 1170)
Length = 310
Score = 32.7 bits (71), Expect = 8.3
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Frame = +2
Query: 224 RHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAF-KLSFDSPQTSSSTGTCKLISARTT 391
+ A TL + A+ K+ +SP +++S GDN + + S S TSSS + A T+
Sbjct: 247 KSAATLVYTASDKIKGESPASTTSNGDNGSVWGSTSTSSSSTSSSAASASSTEASTS 303
>UniRef50_Q09624 Cluster: Uncharacterized protein ZK945.9; n=3;
root|Rep: Uncharacterized protein ZK945.9 -
Caenorhabditis elegans
Length = 3178
Score = 32.7 bits (71), Expect = 8.3
Identities = 28/95 (29%), Positives = 39/95 (41%)
Frame = +2
Query: 143 VDSRPTASQHRSLNRTIAADSPRFLTLRHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAFK 322
V S T+S S T + T + A S P +SSST + A+
Sbjct: 458 VTSTVTSSSSSSTTVTTPTSTESTSTSPSSTVTTSTTAPSTSTTGPSSSSSTPSSTASSS 517
Query: 323 LSFDSPQTSSSTGTCKLISARTTITKYDTTSSLGG 427
+S + T SST T +T TK +TT+S G
Sbjct: 518 VSSTASSTQSSTST----QQSSTTTKSETTTSSDG 548
>UniRef50_Q13620 Cluster: Cullin-4B; n=95; Eukaryota|Rep: Cullin-4B
- Homo sapiens (Human)
Length = 895
Score = 32.7 bits (71), Expect = 8.3
Identities = 24/68 (35%), Positives = 31/68 (45%)
Frame = +2
Query: 155 PTASQHRSLNRTIAADSPRFLTLRHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFD 334
PT S + AA R T + T P +A K L+S +SSS N + FD
Sbjct: 3 PTGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNE---REDFD 59
Query: 335 SPQTSSST 358
S +SSST
Sbjct: 60 STSSSSST 67
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 626,637,310
Number of Sequences: 1657284
Number of extensions: 12341286
Number of successful extensions: 46927
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 42291
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46317
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 51652897375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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