BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0270 (672 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 29 0.61 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.81 SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|c... 28 1.1 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.4 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 1.4 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.5 SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 27 3.3 SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 27 3.3 SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 3.3 SPBC16G5.06 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 3.3 SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 26 4.3 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 26 4.3 SPAC607.03c |snu13||U3 snoRNP-associated protein Snu13|Schizosac... 26 5.7 SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 5.7 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 5.7 SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 25 7.5 SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 25 7.5 SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce... 25 9.9 SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 9.9 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 29.1 bits (62), Expect = 0.61 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 1/60 (1%) Frame = +1 Query: 100 SATTIPAF-SQIKVSRRQSSHSKPASQPQQDHRSRLTALPDPTACTYTASSRSIQAQSRL 276 S T +PA S ++ +QSS S P QP + P P A +Y S++ + L Sbjct: 290 STTPLPAAASYTSMNMKQSSASHPVLQPPAPSTLQFNPSPSPAAPSYPPVDASVKQAADL 349 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 28.7 bits (61), Expect = 0.81 Identities = 21/88 (23%), Positives = 41/88 (46%) Frame = +2 Query: 155 PTASQHRSLNRTIAADSPRFLTLRHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFD 334 PT++ L+ + S + + TL + S+ P TSSS+ ++ S Sbjct: 202 PTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSI--PSTSSSSSSTSSSLSSSSS 259 Query: 335 SPQTSSSTGTCKLISARTTITKYDTTSS 418 S SSS+ + +IS+ ++ + T++S Sbjct: 260 SSTASSSSSSSSIISSSSSSSSSPTSTS 287 Score = 26.2 bits (55), Expect = 4.3 Identities = 18/70 (25%), Positives = 34/70 (48%) Frame = +3 Query: 126 ADKSKSSTVVPQQASIAASTGPSQPTHRAS*PYGMHLHCLIAQHSSSVSTHRRLRAALAT 305 A S SS+ + + +++S+ PS + + + + SSS ST L ++ ++ Sbjct: 201 APTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSS 260 Query: 306 ITQHSSSVST 335 T SSS S+ Sbjct: 261 STASSSSSSS 270 Score = 26.2 bits (55), Expect = 4.3 Identities = 31/125 (24%), Positives = 50/125 (40%), Gaps = 1/125 (0%) Frame = +2 Query: 47 PATEQSQPSD*SSAHQELRLPQSPHSRR*K*VVDSRPTASQH-RSLNRTIAADSPRFLTL 223 P+T S S SS + S ++ S ++S S + TI++ S + Sbjct: 241 PSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSS----SS 296 Query: 224 RHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKY 403 +PT S S SS+ + + SF S TSSS+ S+ ++ + Sbjct: 297 SSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSFS 356 Query: 404 DTTSS 418 TTSS Sbjct: 357 STTSS 361 Score = 25.0 bits (52), Expect = 9.9 Identities = 17/70 (24%), Positives = 38/70 (54%), Gaps = 1/70 (1%) Frame = +3 Query: 135 SKSSTVVPQQASIAASTGPSQPTH-RAS*PYGMHLHCLIAQHSSSVSTHRRLRAALATIT 311 S SST+ +S ++S+ S + ++S + + + SS++++ + A+ + Sbjct: 338 SSSSTISSSSSSPSSSSFSSTTSSSKSSSSFSSTVSSSSSTSSSTLTSSSSSSSRPASSS 397 Query: 312 QHSSSVSTHR 341 HSSS+S+H+ Sbjct: 398 SHSSSLSSHK 407 >SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 28.3 bits (60), Expect = 1.1 Identities = 11/35 (31%), Positives = 17/35 (48%) Frame = -2 Query: 569 DICSVDRRSECCCSELVYMRFILKMS*ARFRHLSI 465 D+C+V R CC+ +++ L F HL I Sbjct: 448 DLCNVSRAGGGCCTAAAFIKCFLAQKDLSFAHLDI 482 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 27.9 bits (59), Expect = 1.4 Identities = 22/69 (31%), Positives = 32/69 (46%), Gaps = 3/69 (4%) Frame = +2 Query: 221 LRHAPTLPHRA--AFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSST-GTCKLISARTT 391 L PTL RA ++ + S +SS T + A+ L+ S SSST T ++ Sbjct: 49 LSSVPTLLKRATTSYNYNTSSASSSSLTSSSAASSSLTSSSSLASSSTNSTTSASPTSSS 108 Query: 392 ITKYDTTSS 418 +T TSS Sbjct: 109 LTSSSATSS 117 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 27.9 bits (59), Expect = 1.4 Identities = 20/81 (24%), Positives = 37/81 (45%) Frame = +2 Query: 155 PTASQHRSLNRTIAADSPRFLTLRHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFD 334 PT S + + +I L+ PT+P + S+ P TS+S+ D +++ L Sbjct: 223 PTTSTSCTTSTSIPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSS-PLPTT 281 Query: 335 SPQTSSSTGTCKLISARTTIT 397 S ++ST ++ T +T Sbjct: 282 STSCTTSTSIPPTGNSTTPVT 302 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.1 bits (57), Expect = 2.5 Identities = 15/48 (31%), Positives = 29/48 (60%) Frame = +2 Query: 275 SPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKYDTTSS 418 S +SSS+ + ++ K S S +SSS+ + K S+ ++ +K ++SS Sbjct: 156 SSSSSSSSSPSSSSSKSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSS 203 >SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 772 Score = 26.6 bits (56), Expect = 3.3 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 6/54 (11%) Frame = +1 Query: 178 PQQDHR---SRLTALP-DPTACTYTASSRSIQAQSRLTADFEQ--HWRQSRSIQ 321 P DH S + LP DP + +Y ASS+ + +LT+ ++ W S S+Q Sbjct: 197 PALDHAVSSSSSSDLPSDPNSASYIASSKQKASSLKLTSSLKKFYSWTSSSSLQ 250 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 26.6 bits (56), Expect = 3.3 Identities = 18/51 (35%), Positives = 25/51 (49%) Frame = +2 Query: 266 SLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTITKYDTTSS 418 S DS SSS + + S DS TSSS+ + S+ + DTT+S Sbjct: 276 SSDSEDDSSSDSSDSESESSSEDSDSTSSSSDSDSSSSSEDGNSNTDTTTS 326 >SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 26.6 bits (56), Expect = 3.3 Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = -1 Query: 525 TCIYALYLE--NVVSEIQASVNYLALYDGYPPHH 430 +CI L+L+ + +I+ + L LYD YPP H Sbjct: 506 SCIKRLHLKLSKGIMDIKVLTHQLMLYDLYPPAH 539 >SPBC16G5.06 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 26.6 bits (56), Expect = 3.3 Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 4/67 (5%) Frame = +2 Query: 233 PTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKL----ISARTTITK 400 P+L RA+ S S S+S + S S TSSS+GT + +SA I Sbjct: 46 PSLYRRASTSSSSSSSSISTSHDSQPSTSSSSPSSTSTSSSSGTSVITASDVSASNEIIS 105 Query: 401 YDTTSSL 421 T +S+ Sbjct: 106 SSTNNSI 112 >SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 26.2 bits (55), Expect = 4.3 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +1 Query: 220 PTACTYTASSRSIQAQSRLTADFEQHWRQ 306 P AC + AS S +++ LTA + +++Q Sbjct: 77 PVACLFVASLNSSRSEEELTATVKDYFQQ 105 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 26.2 bits (55), Expect = 4.3 Identities = 16/57 (28%), Positives = 25/57 (43%) Frame = +2 Query: 182 NRTIAADSPRFLTLRHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSS 352 N+ AD F T+R + T R S D+ + + + +N A + DSP S Sbjct: 115 NKYQDADVDEFNTIRASETASQRPPIPFSSDTLKKTYLSSENDARYPSVSDSPYCES 171 >SPAC607.03c |snu13||U3 snoRNP-associated protein Snu13|Schizosaccharomyces pombe|chr 1|||Manual Length = 125 Score = 25.8 bits (54), Expect = 5.7 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = +3 Query: 108 HNPRILADKSKSSTVVPQQASIAASTGPSQPTHRAS 215 H P + DK+ VP +A++ + G S+P AS Sbjct: 65 HLPLLCEDKNVPYVFVPSKAALGRACGVSRPVISAS 100 >SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 773 Score = 25.8 bits (54), Expect = 5.7 Identities = 18/76 (23%), Positives = 35/76 (46%), Gaps = 1/76 (1%) Frame = +3 Query: 108 HNPRILADKSKSSTVVPQQASIAASTGPSQPTHRAS*PYGMHLHCLIAQHSSSVS-THRR 284 +NP+ + S +++ VP+ S + S + T Y +H+ +I + S S + RR Sbjct: 198 NNPKEIEMNSDTTSSVPKSGSTSFSANVTSSTSMVYLLYSVHVDKIIQYYVDSFSVSERR 257 Query: 285 LRAALATITQHSSSVS 332 L A + + + S Sbjct: 258 LVNTRAVVLKEVQAPS 273 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 25.8 bits (54), Expect = 5.7 Identities = 21/93 (22%), Positives = 43/93 (46%) Frame = +2 Query: 140 VVDSRPTASQHRSLNRTIAADSPRFLTLRHAPTLPHRAAFKLSLDSPQTSSSTGDNHAAF 319 V+ + ++S S + ++ SP + T P ++ S S +SSS+ + ++ Sbjct: 122 VLQTTVSSSSVSSTTSSSSSSSPSSSSTT-TTTSPSSSSSSSSSSSSSSSSSSSSSSSSS 180 Query: 320 KLSFDSPQTSSSTGTCKLISARTTITKYDTTSS 418 S S +SSS+ + S+ ++ + TSS Sbjct: 181 SSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSS 213 >SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces pombe|chr 2|||Manual Length = 342 Score = 25.4 bits (53), Expect = 7.5 Identities = 25/94 (26%), Positives = 39/94 (41%), Gaps = 6/94 (6%) Frame = +2 Query: 155 PTASQHRSLNRTIAADSPRFLTLRH------APTLPHRAAFKLSLDSPQTSSSTGDNHAA 316 P S R+ +RT++ S +L +RH L H+ ++ S S+ST H Sbjct: 234 PYTSPLRTPSRTLSRSSSHYLWVRHGKLTRSVSLLQHKTPRRIRPKSLSKSNSTPLKH-- 291 Query: 317 FKLSFDSPQTSSSTGTCKLISARTTITKYDTTSS 418 LS P ++ TG +S T +SS Sbjct: 292 --LSAQKPNSNYYTGPPTPVSISNTPENIHPSSS 323 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.4 bits (53), Expect = 7.5 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = +2 Query: 236 TLPHRAAFKLSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGT 364 T PH + + SL+SP + S+ + L DSP + ST T Sbjct: 64 TFPHGSNKQASLESPVSYSNPIPSLTWLSLDGDSPDSLVSTPT 106 >SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces pombe|chr 2|||Manual Length = 415 Score = 25.0 bits (52), Expect = 9.9 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +1 Query: 112 IPAFSQIKVSRRQSSHSKPASQPQQDHRSRLTALP 216 +P S + Q HS PAS P +SR +LP Sbjct: 315 LPTLSAVTSDSAQP-HSLPASMPSSQPQSRSESLP 348 >SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.0 bits (52), Expect = 9.9 Identities = 12/45 (26%), Positives = 23/45 (51%) Frame = +2 Query: 263 LSLDSPQTSSSTGDNHAAFKLSFDSPQTSSSTGTCKLISARTTIT 397 +S P + G+N+A K S + +SSST L ++ +++ Sbjct: 1 MSFQQPSNGAQGGNNNALEKTSSNEATSSSSTQVSSLSASGISVS 45 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,557,776 Number of Sequences: 5004 Number of extensions: 50579 Number of successful extensions: 239 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 218 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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