Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0268
         (627 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    71   1e-13
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit...    62   8e-11
SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo...    27   2.2  
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar...    27   2.9  
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho...    26   5.1  
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar...    26   5.1  
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi...    25   6.8  
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce...    25   6.8  
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su...    25   9.0  
SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi...    25   9.0  
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    25   9.0  
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy...    25   9.0  

>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 32/71 (45%), Positives = 48/71 (67%)
 Frame = +3

Query: 333 LKEDGIQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLV 512
           +K +      DIK+LLLGAG+SGK+TI+KQM++++  GF+    KQYR +++ N I SL 
Sbjct: 64  VKGNSTSGGNDIKVLLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLC 123

Query: 513 AILRAMPNLGI 545
            +L AM N  +
Sbjct: 124 LLLEAMDNSNV 134


>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
           Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 354

 Score = 61.7 bits (143), Expect = 8e-11
 Identities = 29/76 (38%), Positives = 50/76 (65%)
 Frame = +3

Query: 318 QIEKNLKEDGIQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNT 497
           +IEK ++    +  K  K+LLLGA +SGKSTI KQ+KI++++GF+ E+   + PV+  N 
Sbjct: 19  KIEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKILNKNGFSQEEIMTFIPVIRRNL 78

Query: 498 IQSLVAILRAMPNLGI 545
           ++S   +++ +   GI
Sbjct: 79  LESAKTLVKIIVQKGI 94


>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1055

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 13/44 (29%), Positives = 27/44 (61%)
 Frame = +3

Query: 336 KEDGIQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFK 467
           K+D ++A  + ++LL+ A E+G     +  + +HE+G+T  + K
Sbjct: 424 KDDLLKAINEYQVLLIVA-ETGSGKTTQLPQFLHEAGYTKGNKK 466


>SPBC1539.08 |||ADP-ribosylation factor, Arf
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 9/24 (37%), Positives = 20/24 (83%)
 Frame = +3

Query: 360 KDIKLLLLGAGESGKSTIVKQMKI 431
           K++++L+LG   +GK+TI+ ++K+
Sbjct: 20  KEMRILMLGLDAAGKTTILYKLKL 43


>SPBC3E7.01 |fab1|ste12,
           SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
           Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1932

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 14/48 (29%), Positives = 23/48 (47%)
 Frame = -1

Query: 282 MKRSPWL*RARPGSLRAAAPCSSHARGPSLRASTAAHALNRARVRFLT 139
           +K +P       GS++   PC+S       R+    H+LN ++ R LT
Sbjct: 94  LKEAPGFIFRLQGSIKVCRPCASILVNNYSRSQLFNHSLNESKNRDLT 141


>SPAC22F3.05c |alp41||ADP-ribosylation factor
           Alp41|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 186

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 9/20 (45%), Positives = 17/20 (85%)
 Frame = +3

Query: 360 KDIKLLLLGAGESGKSTIVK 419
           +++++LLLG   +GK+TI+K
Sbjct: 15  REVRVLLLGLDNAGKTTILK 34


>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 749

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +1

Query: 385 VLANRARVL*SNR*KLSMKVDSQMKTSNSTGPWS 486
           +LAN  R++ SN  K+    D Q + S   GPW+
Sbjct: 1   MLANALRLV-SNSKKIKYDYDIQKENSVQVGPWT 33


>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1131

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = -3

Query: 445 PLSWIIFICLTIVLLPDSPAPRSRSLMSLAAWMP 344
           P+S  +F+ LTI  L ++    + +   L +WMP
Sbjct: 533 PVSAFVFLELTITALYETKLSPAAAFEKLKSWMP 566


>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 446

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = +3

Query: 366 IKLLLLGAGESGKSTIVKQMKIIHESGFTNE 458
           I +  +G    GKST+VK +  +H   F NE
Sbjct: 24  INIGTIGHVAHGKSTVVKAISGVHTVRFKNE 54


>SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit
           Mcm5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = +3

Query: 327 KNLKEDGIQAAKDIKLLLLGAGESGKSTIVK 419
           K +  DG++   DI +LLLG   + KS  +K
Sbjct: 353 KKILPDGMRLRGDINVLLLGDPGTAKSQFLK 383


>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
 Frame = +3

Query: 444 GFTNEDFKQYRPVVYSNTIQSLVAILRAMPNLGIIYGNRD-RESDGKM 584
           GF +E+ KQYR  +Y+  +  L+ ++  M N  I +  R  +  DG++
Sbjct: 701 GFVDEEMKQYR--LYT-VVPQLLGLIEEMTNWYIRFNRRRLKGEDGEI 745


>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 991

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 7/13 (53%), Positives = 10/13 (76%)
 Frame = -1

Query: 561 LYCHR*CPSWAWH 523
           LY ++ CPSW W+
Sbjct: 626 LYYYQGCPSWTWY 638


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,441,573
Number of Sequences: 5004
Number of extensions: 47153
Number of successful extensions: 137
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)