BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0268
(627 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26300.1 68415.m03156 guanine nucleotide binding protein (G-p... 65 4e-11
At1g31930.2 68414.m03924 extra-large guanine nucleotide binding ... 40 0.002
At1g31930.1 68414.m03923 extra-large guanine nucleotide binding ... 40 0.002
At4g34390.1 68417.m04885 extra-large guanine nucleotide binding ... 37 0.010
At4g24650.1 68417.m03529 adenylate isopentenyltransferase 4 / cy... 27 7.7
>At2g26300.1 68415.m03156 guanine nucleotide binding protein
(G-protein) alpha-1 subunit / GP-alpha-1 (GPA1)
identical to SP|P18064 Guanine nucleotide-binding
protein alpha-1 subunit (GP-alpha-1) {Arabidopsis
thaliana}
Length = 383
Score = 64.9 bits (151), Expect = 4e-11
Identities = 31/65 (47%), Positives = 49/65 (75%), Gaps = 1/65 (1%)
Frame = +3
Query: 318 QIEKNLKEDGIQAAKDI-KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSN 494
+IE+ ++++ +A K I KLLLLGAGESGKSTI KQ+K++ ++GF + K Y PV+++N
Sbjct: 23 EIERRIEQEA-KAEKHIRKLLLLGAGESGKSTIFKQIKLLFQTGFDEGELKSYVPVIHAN 81
Query: 495 TIQSL 509
Q++
Sbjct: 82 VYQTI 86
>At1g31930.2 68414.m03924 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 39.5 bits (88), Expect = 0.002
Identities = 23/58 (39%), Positives = 34/58 (58%)
Frame = +3
Query: 348 IQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAIL 521
I+ K KLLLLG SG STI KQ K ++ + F+ E+ + + +V SN + L +L
Sbjct: 425 IEHKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILL 482
>At1g31930.1 68414.m03923 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 39.5 bits (88), Expect = 0.002
Identities = 23/58 (39%), Positives = 34/58 (58%)
Frame = +3
Query: 348 IQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAIL 521
I+ K KLLLLG SG STI KQ K ++ + F+ E+ + + +V SN + L +L
Sbjct: 425 IEHKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILL 482
>At4g34390.1 68417.m04885 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 861
Score = 37.1 bits (82), Expect = 0.010
Identities = 17/53 (32%), Positives = 32/53 (60%)
Frame = +3
Query: 369 KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRA 527
KLLL+G+ + G +TI KQ + ++ F+ ED ++ + ++ +N L +L A
Sbjct: 464 KLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMVLEA 516
>At4g24650.1 68417.m03529 adenylate isopentenyltransferase 4 /
cytokinin synthase (IPT4) identical to adenylate
isopentenyltransferase (IPT4) [Arabidopsis thaliana]
GI:14279062
Length = 318
Score = 27.5 bits (58), Expect = 7.7
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +3
Query: 363 DIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPN 536
D ++++GA SGKS++ + + ++ N D Q+ + T QS + R +P+
Sbjct: 5 DKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPH 62
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,192,191
Number of Sequences: 28952
Number of extensions: 266533
Number of successful extensions: 756
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 733
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 756
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -