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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0268
         (627 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g26300.1 68415.m03156 guanine nucleotide binding protein (G-p...    65   4e-11
At1g31930.2 68414.m03924 extra-large guanine nucleotide binding ...    40   0.002
At1g31930.1 68414.m03923 extra-large guanine nucleotide binding ...    40   0.002
At4g34390.1 68417.m04885 extra-large guanine nucleotide binding ...    37   0.010
At4g24650.1 68417.m03529 adenylate isopentenyltransferase 4 / cy...    27   7.7  

>At2g26300.1 68415.m03156 guanine nucleotide binding protein
           (G-protein) alpha-1 subunit / GP-alpha-1 (GPA1)
           identical to SP|P18064 Guanine nucleotide-binding
           protein alpha-1 subunit (GP-alpha-1) {Arabidopsis
           thaliana}
          Length = 383

 Score = 64.9 bits (151), Expect = 4e-11
 Identities = 31/65 (47%), Positives = 49/65 (75%), Gaps = 1/65 (1%)
 Frame = +3

Query: 318 QIEKNLKEDGIQAAKDI-KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSN 494
           +IE+ ++++  +A K I KLLLLGAGESGKSTI KQ+K++ ++GF   + K Y PV+++N
Sbjct: 23  EIERRIEQEA-KAEKHIRKLLLLGAGESGKSTIFKQIKLLFQTGFDEGELKSYVPVIHAN 81

Query: 495 TIQSL 509
             Q++
Sbjct: 82  VYQTI 86


>At1g31930.2 68414.m03924 extra-large guanine nucleotide binding
           protein, putative / G-protein, putative similar to
           extra-large G-protein (XLG) [Arabidopsis thaliana]
           GI:3201680; contains Pfam profile PF00503: G-protein
           alpha subunit
          Length = 848

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 23/58 (39%), Positives = 34/58 (58%)
 Frame = +3

Query: 348 IQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAIL 521
           I+  K  KLLLLG   SG STI KQ K ++ + F+ E+ +  + +V SN  + L  +L
Sbjct: 425 IEHKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILL 482


>At1g31930.1 68414.m03923 extra-large guanine nucleotide binding
           protein, putative / G-protein, putative similar to
           extra-large G-protein (XLG) [Arabidopsis thaliana]
           GI:3201680; contains Pfam profile PF00503: G-protein
           alpha subunit
          Length = 848

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 23/58 (39%), Positives = 34/58 (58%)
 Frame = +3

Query: 348 IQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAIL 521
           I+  K  KLLLLG   SG STI KQ K ++ + F+ E+ +  + +V SN  + L  +L
Sbjct: 425 IEHKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILL 482


>At4g34390.1 68417.m04885 extra-large guanine nucleotide binding
           protein, putative / G-protein, putative similar to
           extra-large G-protein (XLG) [Arabidopsis thaliana]
           GI:3201680; contains Pfam profile PF00503: G-protein
           alpha subunit
          Length = 861

 Score = 37.1 bits (82), Expect = 0.010
 Identities = 17/53 (32%), Positives = 32/53 (60%)
 Frame = +3

Query: 369 KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRA 527
           KLLL+G+ + G +TI KQ + ++   F+ ED ++ + ++ +N    L  +L A
Sbjct: 464 KLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMVLEA 516


>At4g24650.1 68417.m03529 adenylate isopentenyltransferase 4 /
           cytokinin synthase (IPT4) identical to adenylate
           isopentenyltransferase (IPT4) [Arabidopsis thaliana]
           GI:14279062
          Length = 318

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = +3

Query: 363 DIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPN 536
           D  ++++GA  SGKS++   + +  ++   N D  Q+   +   T QS +   R +P+
Sbjct: 5   DKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPH 62


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,192,191
Number of Sequences: 28952
Number of extensions: 266533
Number of successful extensions: 756
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 733
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 756
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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