Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0259
(663 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 226 3e-60
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 112 5e-26
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 95 8e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 8e-21
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 91 1e-19
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 4e-12
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 62 5e-11
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 62 9e-11
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 1e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.4
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 5.6
SPCC830.05c |epl1||histone acetyltransferase complex subunit Epl... 25 7.4
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 9.7
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 25 9.7
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 9.7
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 25 9.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 226 bits (552), Expect = 3e-60
Identities = 103/118 (87%), Positives = 109/118 (92%)
Frame = +1
Query: 13 PRALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEI 192
P ALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI
Sbjct: 258 PEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEI 317
Query: 193 TALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 366
ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 318 QALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 112 bits (269), Expect = 5e-26
Identities = 50/118 (42%), Positives = 79/118 (66%)
Frame = +1
Query: 13 PRALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEI 192
P LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +R E+
Sbjct: 263 PEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISEL 322
Query: 193 TALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 366
A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F
Sbjct: 323 RAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 95.1 bits (226), Expect = 8e-21
Identities = 44/96 (45%), Positives = 62/96 (64%), Gaps = 2/96 (2%)
Frame = +1
Query: 58 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII- 231
G+HE Y SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S + +
Sbjct: 323 GLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAE 382
Query: 232 APPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 339
+VW GGSILASL FQ +W+SKQEYDE G
Sbjct: 383 TASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 95.1 bits (226), Expect = 8e-21
Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
Frame = +1
Query: 58 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 237
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 238 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 363
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 91.1 bits (216), Expect = 1e-19
Identities = 50/121 (41%), Positives = 74/121 (61%), Gaps = 12/121 (9%)
Frame = +1
Query: 13 PRALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEI 192
P LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+ R++KEI
Sbjct: 258 PECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEI 317
Query: 193 TAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDES 336
L P+ + K+KI P R+++V+IGG++LA ++ MW+SK E++E
Sbjct: 318 KQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEY 377
Query: 337 G 339
G
Sbjct: 378 G 378
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 66.1 bits (154), Expect = 4e-12
Identities = 29/117 (24%), Positives = 61/117 (52%)
Frame = +1
Query: 1 EIPLPRALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRM 180
+I +P +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R+
Sbjct: 595 QIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETRI 654
Query: 181 QKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 351
++E+T++ P I + W G S + F+ ++++EY E GP +
Sbjct: 655 KRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 62.5 bits (145), Expect = 5e-11
Identities = 35/117 (29%), Positives = 56/117 (47%)
Frame = +1
Query: 10 LPRALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 189
+P LF PS + + GI E S+ +I+ L N V GG +PG R+ E
Sbjct: 283 IPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSE 342
Query: 190 ITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK 360
+ +LAP+ ++K+ P + W S + L + I++ EY E G +I+ RK
Sbjct: 343 LRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 61.7 bits (143), Expect = 9e-11
Identities = 35/130 (26%), Positives = 60/130 (46%), Gaps = 15/130 (11%)
Frame = +1
Query: 13 PRALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 189
P F P + + E N + +D+RK LY N VLSGG+T++ +R+Q++
Sbjct: 290 PEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRD 349
Query: 190 ITALAPST--------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEY 327
+ + + + +I+ ++ +VW GGS+LA F +K +Y
Sbjct: 350 LKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADY 409
Query: 328 DESGPSIVHR 357
+E G SI R
Sbjct: 410 EEYGASIARR 419
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 1e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = +1
Query: 112 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 282
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 283 SLSTFQQMWISKQEYDESGPSIVHRK 360
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = +1
Query: 259 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 360
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 5.6
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = +1
Query: 184 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 294
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPCC830.05c |epl1||histone acetyltransferase complex subunit Epl1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 25.4 bits (53), Expect = 7.4
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +3
Query: 3 DSVAQSSLPTLVLGYGSLRHPRDHI*LHHEVRRG 104
DS++ SS +L GY +L HPR+ I + + RG
Sbjct: 461 DSLSFSSNNSLY-GYSTLLHPRNPICVRQRIGRG 493
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 251 TPYGSVDRSSPPSLPSNKCGSRNKSTTSLAPP 346
TP + PPS+PS++ R S ++ APP
Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 25.0 bits (52), Expect = 9.7
Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%)
Frame = +2
Query: 257 YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 343
Y + SSPP P N G + N +TTS P
Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -1
Query: 318 FRDPHLLEGREGGEDRSTDPYGVLPLWRS 232
F HLL + G+D + + YG L W S
Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSWIS 825
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 25.0 bits (52), Expect = 9.7
Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%)
Frame = +2
Query: 257 YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 343
Y + SSPP P N G + N +TTS P
Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,548,020
Number of Sequences: 5004
Number of extensions: 50083
Number of successful extensions: 155
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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