SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0248
         (687 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|S...   132   6e-32
SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch...   124   2e-29
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      27   1.9  
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k...    26   4.4  
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac...    26   5.9  

>SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein
           L25|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 141

 Score =  132 bits (318), Expect = 6e-32
 Identities = 66/110 (60%), Positives = 77/110 (70%)
 Frame = +1

Query: 337 VTKALKAQRKVVKGEHGKRVRKIRNSVHFRRPKTFEPPRHPKYPRKSLPKRNRMDAYNII 516
           V KA  AQ+ V KG H K  RK+R S  FRRPKT E  R PKY RKS+P  +R+D Y II
Sbjct: 3   VGKAKGAQKTVQKGIHNKVARKVRTSTTFRRPKTLELARKPKYARKSVPHASRLDEYKII 62

Query: 517 KFPLTSEAAMKKIEDNNTLVFIVHTSANKHHIKAAVKKLYDINVAKVNTL 666
             P+ SE+AMKKIED+NTLVF VH  ANK  IK AVKKLY ++  K+NTL
Sbjct: 63  VNPINSESAMKKIEDDNTLVFHVHLKANKFTIKNAVKKLYSVDAVKINTL 112


>SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein
           L25|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 141

 Score =  124 bits (298), Expect = 2e-29
 Identities = 62/110 (56%), Positives = 74/110 (67%)
 Frame = +1

Query: 337 VTKALKAQRKVVKGEHGKRVRKIRNSVHFRRPKTFEPPRHPKYPRKSLPKRNRMDAYNII 516
           V KA  AQ+ V KG H K  +K+R S  FRRPKT +  R PKY RKS+    R+D Y II
Sbjct: 3   VAKAKGAQKTVQKGIHNKVAKKVRTSTTFRRPKTLQLSRKPKYARKSVAHAPRLDEYKII 62

Query: 517 KFPLTSEAAMKKIEDNNTLVFIVHTSANKHHIKAAVKKLYDINVAKVNTL 666
             P+ SE+AMKKIED+NTLVF VH  ANK  IK AV+KLY +   K+NTL
Sbjct: 63  VNPINSESAMKKIEDDNTLVFHVHLKANKFTIKEAVRKLYSVEPVKINTL 112


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 19/70 (27%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
 Frame = -1

Query: 501 IHAISLRQRLPWVFRVPR--RFKRLGSAEMHRVANFPYSLPMFTFYNLPLSLKCFSNRFH 328
           + A  +  R P   R P+  RF+ L S++   + + PY+ P   F     S +    + H
Sbjct: 196 LRASPVSSRTPSPTRFPKHARFQTLNSSDSASIYSSPYTSPTLEFSKKDASARSDILKMH 255

Query: 327 YFFLSLNPSL 298
               S  PSL
Sbjct: 256 RRTKSATPSL 265


>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
           kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 836

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = +3

Query: 237 SQNCQTQASSFQIEDCTEAQKDWD*GTKKS 326
           S+NCQ  +    +++C EA  D D G  K+
Sbjct: 479 SENCQKLSKQIPLDECNEALFDDDNGDYKA 508


>SPAC18G6.05c |||translation elongation regulator Gcn1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2670

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 9/51 (17%), Positives = 25/51 (49%)
 Frame = +1

Query: 496  MDAYNIIKFPLTSEAAMKKIEDNNTLVFIVHTSANKHHIKAAVKKLYDINV 648
            + A+++ +F    E  ++  +DN T   I H  + ++ + A     +++ +
Sbjct: 1090 LQAFDLSRFEFIKEIFLELYDDNETNASIAHQISTQNGLDATETSFFELQI 1140


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,005,142
Number of Sequences: 5004
Number of extensions: 38005
Number of successful extensions: 118
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -