Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0244
(613 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48440.2 68418.m05990 FAD-dependent oxidoreductase family pro... 33 0.15
At5g48440.1 68418.m05989 FAD-dependent oxidoreductase family pro... 33 0.15
At5g34358.1 68418.m04025 hypothetical protein 32 0.34
At1g12170.1 68414.m01409 F-box family protein contains F-box dom... 29 1.8
At5g63750.1 68418.m08001 IBR domain-containing protein contains ... 29 3.2
At5g62570.1 68418.m07852 calmodulin-binding protein similar to c... 28 4.2
At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)... 28 4.2
At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)... 28 4.2
At5g18350.1 68418.m02159 disease resistance protein (TIR-NBS-LRR... 28 5.6
At3g12570.3 68416.m01566 expressed protein 28 5.6
At3g12570.2 68416.m01565 expressed protein 28 5.6
At3g12570.1 68416.m01564 expressed protein 28 5.6
At2g43070.1 68415.m05344 protease-associated (PA) domain-contain... 28 5.6
At1g36150.1 68414.m04494 protease inhibitor/seed storage/lipid t... 28 5.6
At5g63710.1 68418.m07997 leucine-rich repeat transmembrane prote... 27 7.4
At4g01640.1 68417.m00213 hypothetical protein 27 7.4
At2g34280.1 68415.m04194 S locus F-box-related / SLF-related con... 27 7.4
At3g29810.1 68416.m03794 phytochelatin synthetase family protein... 27 9.8
At2g28240.1 68415.m03428 hydroxyproline-rich glycoprotein family... 27 9.8
At1g77010.1 68414.m08968 pentatricopeptide (PPR) repeat-containi... 27 9.8
>At5g48440.2 68418.m05990 FAD-dependent oxidoreductase family
protein low similarity to hydrogen cyanide synthase HcnC
from Pseudomonas fluorescens [GI:3220203]; contains Pfam
profile PF01266 FAD dependent oxidoreductase
Length = 333
Score = 33.1 bits (72), Expect = 0.15
Identities = 17/69 (24%), Positives = 31/69 (44%)
Frame = +3
Query: 255 GWKQCGSLLLARTRDRMTVYRRMKSQSVSWSIDCDLVTPKKCHELFPMLNVEDVLGGLWI 434
GWK+ GSLL+ RT + ++ + + + ++ + P + V D G ++
Sbjct: 40 GWKKTGSLLIGRTTEECVALKQKVHELSEAGLRTEYLSSAELLLKEPAILVNDNTGAAFL 99
Query: 435 PGDGVGDPH 461
P D D H
Sbjct: 100 PDDSQLDAH 108
>At5g48440.1 68418.m05989 FAD-dependent oxidoreductase family
protein low similarity to hydrogen cyanide synthase HcnC
from Pseudomonas fluorescens [GI:3220203]; contains Pfam
profile PF01266 FAD dependent oxidoreductase
Length = 367
Score = 33.1 bits (72), Expect = 0.15
Identities = 17/69 (24%), Positives = 31/69 (44%)
Frame = +3
Query: 255 GWKQCGSLLLARTRDRMTVYRRMKSQSVSWSIDCDLVTPKKCHELFPMLNVEDVLGGLWI 434
GWK+ GSLL+ RT + ++ + + + ++ + P + V D G ++
Sbjct: 40 GWKKTGSLLIGRTTEECVALKQKVHELSEAGLRTEYLSSAELLLKEPAILVNDNTGAAFL 99
Query: 435 PGDGVGDPH 461
P D D H
Sbjct: 100 PDDSQLDAH 108
>At5g34358.1 68418.m04025 hypothetical protein
Length = 116
Score = 31.9 bits (69), Expect = 0.34
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = -1
Query: 493 VASLIRDIQSKCGSPTPSPGIHRPPSTSST 404
+A+ I+D QSK P+P P + R STSST
Sbjct: 1 MAAQIKDFQSKPRPPSPIPKVRRSGSTSST 30
>At1g12170.1 68414.m01409 F-box family protein contains F-box domain
Pfam:PF00646
Length = 364
Score = 29.5 bits (63), Expect = 1.8
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +2
Query: 509 CDGRLFCDSCAIERRQSVWS 568
CDG LFCDS + ++ ++W+
Sbjct: 98 CDGLLFCDSWSWRKKAAIWN 117
>At5g63750.1 68418.m08001 IBR domain-containing protein contains a
Prosite:PS00518 Zinc finger, C3HC4 type (RING finger),
signature and Pfam:PF01485 IBR domain
Length = 536
Score = 28.7 bits (61), Expect = 3.2
Identities = 20/49 (40%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
Frame = +2
Query: 446 CRRPAFALYVSYERSN**RCGCDGRLFCDSCAI-ERRQSVWSGNYKWCN 589
C RPA Y R C C+GR FC C E SG YK+CN
Sbjct: 280 CLRPADLGTKQYLRFL--TCACNGR-FCWKCMQPEEAHKTESGFYKFCN 325
>At5g62570.1 68418.m07852 calmodulin-binding protein similar to
calmodulin-binding protein TCB60 GI:1698548 from
[Nicotiana tabacum]
Length = 487
Score = 28.3 bits (60), Expect = 4.2
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = -1
Query: 268 HCFHPVGLPRASSSLRSLIEDWASLT*ASVGLNAPTR 158
+C H +GL S L+S ++D+ S ASVG A ++
Sbjct: 428 NCSHILGLEEPQSELQSALDDFMSQKNASVGGKAHSK 464
>At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)
identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
Length = 498
Score = 28.3 bits (60), Expect = 4.2
Identities = 26/78 (33%), Positives = 42/78 (53%), Gaps = 7/78 (8%)
Frame = +3
Query: 360 LVTPKKCHELFPMLNVEDVL--GGLWIPGD--GVGDP-HLLCMSLMR--EATDKGVGVME 518
L T + C EL LN++ ++ GG + D + P HLL + R + T KGV V++
Sbjct: 206 LQTSQVCKELSKYLNIQVMVTTGGTSLRDDIMRLHQPVHLLVGTPGRILDLTKKGVCVLK 265
Query: 519 DCSVTAVLSKDDKVSGVE 572
DC++ V+ + DK+ E
Sbjct: 266 DCAM-LVMDEADKLLSAE 282
>At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)
identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
Length = 498
Score = 28.3 bits (60), Expect = 4.2
Identities = 26/78 (33%), Positives = 42/78 (53%), Gaps = 7/78 (8%)
Frame = +3
Query: 360 LVTPKKCHELFPMLNVEDVL--GGLWIPGD--GVGDP-HLLCMSLMR--EATDKGVGVME 518
L T + C EL LN++ ++ GG + D + P HLL + R + T KGV V++
Sbjct: 206 LQTSQVCKELSKYLNIQVMVTTGGTSLRDDIMRLHQPVHLLVGTPGRILDLTKKGVCVLK 265
Query: 519 DCSVTAVLSKDDKVSGVE 572
DC++ V+ + DK+ E
Sbjct: 266 DCAM-LVMDEADKLLSAE 282
>At5g18350.1 68418.m02159 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1193
Score = 27.9 bits (59), Expect = 5.6
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +3
Query: 222 LKELEARGRPTGWKQCGSLLLARTRDR 302
L++L+A + TGW GS ++ T+DR
Sbjct: 322 LEQLDAMAKETGWFGYGSRIIITTQDR 348
>At3g12570.3 68416.m01566 expressed protein
Length = 489
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 8/57 (14%)
Frame = +2
Query: 251 HRVETMWFTIIGKNKRPHDCL--------QKNEKSVCIVEHRL*LSYTKEMS*IISN 397
H V+T+ + G NKR DCL +EKS+ + H L ++ + S ++ N
Sbjct: 300 HAVDTVEVQLAGSNKRKRDCLALGNCDDSSSSEKSLDMKIHATELPWSNDFSGVMKN 356
>At3g12570.2 68416.m01565 expressed protein
Length = 489
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 8/57 (14%)
Frame = +2
Query: 251 HRVETMWFTIIGKNKRPHDCL--------QKNEKSVCIVEHRL*LSYTKEMS*IISN 397
H V+T+ + G NKR DCL +EKS+ + H L ++ + S ++ N
Sbjct: 300 HAVDTVEVQLAGSNKRKRDCLALGNCDDSSSSEKSLDMKIHATELPWSNDFSGVMKN 356
>At3g12570.1 68416.m01564 expressed protein
Length = 489
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 8/57 (14%)
Frame = +2
Query: 251 HRVETMWFTIIGKNKRPHDCL--------QKNEKSVCIVEHRL*LSYTKEMS*IISN 397
H V+T+ + G NKR DCL +EKS+ + H L ++ + S ++ N
Sbjct: 300 HAVDTVEVQLAGSNKRKRDCLALGNCDDSSSSEKSLDMKIHATELPWSNDFSGVMKN 356
>At2g43070.1 68415.m05344 protease-associated (PA) domain-containing
protein contains protease associated (PA) domain,
Pfam:PF02225
Length = 540
Score = 27.9 bits (59), Expect = 5.6
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +1
Query: 91 F*FSLIFLYCEEWVQGVAGIHQALLEHSSQHLLR*GLPSPQLGFLRSLRLVV 246
F + L +C +QG+ I A++ +HL R + P LG + L L+V
Sbjct: 278 FVWVLTIFFCIGGMQGMHNIIMAVILRKCRHLARKSVKLPLLGTMSVLSLLV 329
>At1g36150.1 68414.m04494 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein low similarity to
glucoamylase S1/S2 [Precursor] from Saccharomyces
cerevisiae [SP|P08640], proteophosphoglycan from
Leishmania major [GI:5420387]; contains Pfam protease
inhibitor/seed storage/LTP family domain PF00234
Length = 256
Score = 27.9 bits (59), Expect = 5.6
Identities = 23/67 (34%), Positives = 31/67 (46%), Gaps = 2/67 (2%)
Frame = -1
Query: 598 SHSIAPFVVSTPDTLSSFDSTAVTEQSSITP--TPLSVASLIRDIQSKCGSPTPSPGIHR 425
SHS P S+P T S S AV+ S + +P+ S + SP+PSP
Sbjct: 173 SHSSPPVSHSSPPTSRS--SPAVSHSSPVVAASSPVKAVSSSTASSPRAASPSPSPS--- 227
Query: 424 PPSTSST 404
PS SS+
Sbjct: 228 -PSISSS 233
>At5g63710.1 68418.m07997 leucine-rich repeat transmembrane protein
kinase, putative
Length = 614
Score = 27.5 bits (58), Expect = 7.4
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 149 FIRPCWSIQANTCSGEACPVLN*AS*GA*GS 241
F+ PC+S TC G++ LN AS G G+
Sbjct: 77 FVSPCYSWSYVTCRGQSVVALNLASSGFTGT 107
>At4g01640.1 68417.m00213 hypothetical protein
Length = 300
Score = 27.5 bits (58), Expect = 7.4
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = +3
Query: 276 LLLARTRDRMTVYRRMKSQSVSWSIDCDLVTPKKCHELFPMLNVEDVLGG 425
L++ DR+ V +M V WS+D D T K+ + + LN+ L G
Sbjct: 190 LIMCNLDDRLCVSEKMWPNQVIWSLDSDHKTWKEIYSI--DLNITSSLFG 237
>At2g34280.1 68415.m04194 S locus F-box-related / SLF-related
contains Pfam PF00646: F-box domain; contains weak hit
to TIGRFAM TIGR01640 : F-box protein interaction domain;
weakly similar to self-incompatibility (S-) locus F-box
(GI:29420811) [Prunus mume]
Length = 391
Score = 27.5 bits (58), Expect = 7.4
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = +3
Query: 276 LLLARTRDRMTVYRRMKSQSVSWSIDCDLVTPKKCHELFPMLNVEDVLGG 425
L++ DR+ V +M V WS+D D T K+ + + LN+ L G
Sbjct: 281 LIMCNLDDRLCVSEKMWPNQVIWSLDSDHKTWKEIYSI--DLNITSSLFG 328
>At3g29810.1 68416.m03794 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL2 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 441
Score = 27.1 bits (57), Expect = 9.8
Identities = 18/81 (22%), Positives = 39/81 (48%), Gaps = 3/81 (3%)
Frame = +3
Query: 123 RVGAGSRWHS--SGLVGAFKPTLAQVRLAQSSIRLLKELEARGRPTGWKQCGSLLLARTR 296
R G S W + + +F+ ++ Q +++R + + + G+ CG L +
Sbjct: 133 RGGVISAWAQDPATAISSFQISVGQSGTTNTTVRAPRNITLKAPGPGYT-CGPAKLVKPS 191
Query: 297 DRMTVYRRMKSQSV-SWSIDC 356
++ +R K+QS+ +W+I C
Sbjct: 192 RFISADKRRKTQSLLTWNITC 212
>At2g28240.1 68415.m03428 hydroxyproline-rich glycoprotein family
protein
Length = 660
Score = 27.1 bits (57), Expect = 9.8
Identities = 19/60 (31%), Positives = 24/60 (40%)
Frame = -1
Query: 520 SSITPTPLSVASLIRDIQSKCGSPTPSPGIHRPPSTSSTFSIGNNS*HFFGVTKSQSMLH 341
S+ P PL A QS C +P P P +P S T I + + VT LH
Sbjct: 475 STALPRPLVTAEAPPLHQSSCKAPQPKPISQQPAVQSKTDIINSTALPRPSVTTEARPLH 534
>At1g77010.1 68414.m08968 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 695
Score = 27.1 bits (57), Expect = 9.8
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +3
Query: 213 IRLLKELEARGRPTGWKQCGSLLLAR 290
+RLL+ +R R T W+Q LLL +
Sbjct: 30 VRLLQSCSSRNRETLWRQTNGLLLKK 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,033,277
Number of Sequences: 28952
Number of extensions: 295349
Number of successful extensions: 897
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 865
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 897
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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