SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0242
         (543 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q6L4B3 Cluster: Polyprotein, putative; n=6; core eudico...    72   1e-11
UniRef50_Q1AKH8 Cluster: Reverse transcriptase family member; n=...    72   1e-11
UniRef50_Q2HTD6 Cluster: RNA-binding region RNP-1 (RNA recogniti...    66   7e-10
UniRef50_Q4TE65 Cluster: Chromosome undetermined SCAF5629, whole...    59   6e-08
UniRef50_Q6L3J9 Cluster: Polyprotein, putative; n=1; Solanum dem...    43   0.005
UniRef50_UPI0000E490F6 Cluster: PREDICTED: similar to endonuclea...    38   0.11 
UniRef50_Q4Z066 Cluster: Putative uncharacterized protein; n=1; ...    37   0.26 
UniRef50_UPI0000E4958A Cluster: PREDICTED: similar to endonuclea...    36   0.60 
UniRef50_Q6ZRM1 Cluster: CDNA FLJ46255 fis, clone TESTI4023172; ...    36   0.60 
UniRef50_A7S7P6 Cluster: Predicted protein; n=1; Nematostella ve...    35   1.1  
UniRef50_Q7NBW8 Cluster: Putative uncharacterized protein; n=1; ...    35   1.4  
UniRef50_O96121 Cluster: Putative uncharacterized protein PFB008...    34   1.8  
UniRef50_UPI00004DBC97 Cluster: UPI00004DBC97 related cluster; n...    34   2.4  
UniRef50_A4KWG0 Cluster: Reverse transcriptase; n=3; Ostrinia nu...    33   3.2  
UniRef50_UPI0000ECB802 Cluster: UPI0000ECB802 related cluster; n...    33   4.3  
UniRef50_Q7R8T3 Cluster: Streptococcus pyogenes AMV156; n=6; Pla...    33   5.6  
UniRef50_A5E0Q3 Cluster: Putative uncharacterized protein; n=2; ...    33   5.6  
UniRef50_UPI000069E6CB Cluster: UPI000069E6CB related cluster; n...    32   7.4  
UniRef50_Q8IBB3 Cluster: Leucine-tRNA ligase; n=3; cellular orga...    32   7.4  
UniRef50_UPI0000D8F084 Cluster: UPI0000D8F084 related cluster; n...    32   9.8  
UniRef50_Q5QZE5 Cluster: Uncharacterized conserved membrane prot...    32   9.8  

>UniRef50_Q6L4B3 Cluster: Polyprotein, putative; n=6; core
           eudicotyledons|Rep: Polyprotein, putative - Solanum
           demissum (Wild potato)
          Length = 868

 Score = 71.7 bits (168), Expect = 1e-11
 Identities = 32/56 (57%), Positives = 38/56 (67%)
 Frame = +2

Query: 320 HERGVHAAEMRMLRWMCGVTRMDKIKNEYSRGSAKVAPVINKLKSGRLAWCGYVMR 487
           H   +H AEMRMLRWMCG TR DKI+NE  R    VA V++KL+  RL W G+V R
Sbjct: 575 HVHKMHVAEMRMLRWMCGHTRSDKIRNEVIREKVGVASVVDKLREARLRWFGHVKR 630


>UniRef50_Q1AKH8 Cluster: Reverse transcriptase family member; n=6;
           Papilionoideae|Rep: Reverse transcriptase family member
           - Glycine max (Soybean)
          Length = 377

 Score = 71.7 bits (168), Expect = 1e-11
 Identities = 32/57 (56%), Positives = 38/57 (66%)
 Frame = +2

Query: 317 KHERGVHAAEMRMLRWMCGVTRMDKIKNEYSRGSAKVAPVINKLKSGRLAWCGYVMR 487
           +HE  V  AEMRMLRWMCG TR DKI+NE  R    VAP++ K+   RL W G+V R
Sbjct: 258 QHENKVGVAEMRMLRWMCGKTRQDKIRNEAIRERVGVAPIVEKMVENRLRWFGHVER 314


>UniRef50_Q2HTD6 Cluster: RNA-binding region RNP-1 (RNA recognition
           motif); Calcium-binding EF- hand; n=1; Medicago
           truncatula|Rep: RNA-binding region RNP-1 (RNA
           recognition motif); Calcium-binding EF- hand - Medicago
           truncatula (Barrel medic)
          Length = 559

 Score = 65.7 bits (153), Expect = 7e-10
 Identities = 30/57 (52%), Positives = 37/57 (64%)
 Frame = +2

Query: 317 KHERGVHAAEMRMLRWMCGVTRMDKIKNEYSRGSAKVAPVINKLKSGRLAWCGYVMR 487
           +HE  V  AEMRMLRWM G TR D+I+N+  R    VAP++ KL   RL W G+V R
Sbjct: 300 QHENQVSVAEMRMLRWMSGKTRHDRIRNDTIRERVGVAPIVEKLVENRLRWFGHVER 356


>UniRef50_Q4TE65 Cluster: Chromosome undetermined SCAF5629, whole
           genome shotgun sequence; n=3; Tetraodon
           nigroviridis|Rep: Chromosome undetermined SCAF5629,
           whole genome shotgun sequence - Tetraodon nigroviridis
           (Green puffer)
          Length = 73

 Score = 59.3 bits (137), Expect = 6e-08
 Identities = 28/59 (47%), Positives = 39/59 (66%)
 Frame = +2

Query: 317 KHERGVHAAEMRMLRWMCGVTRMDKIKNEYSRGSAKVAPVINKLKSGRLAWCGYVMRXE 493
           + E  +  AEM+MLR+  GVTRMD+IK EY RG+A V    +K++  RL W G+V R +
Sbjct: 13  RQETELEVAEMKMLRFSLGVTRMDEIKKEYIRGTAHVRCFGDKVRETRLRWFGHVQRTD 71


>UniRef50_Q6L3J9 Cluster: Polyprotein, putative; n=1; Solanum
           demissum|Rep: Polyprotein, putative - Solanum demissum
           (Wild potato)
          Length = 139

 Score = 42.7 bits (96), Expect = 0.005
 Identities = 19/41 (46%), Positives = 25/41 (60%)
 Frame = +2

Query: 341 AEMRMLRWMCGVTRMDKIKNEYSRGSAKVAPVINKLKSGRL 463
           AEMRM RWMC  TR DKI N+       +  V++K++  RL
Sbjct: 98  AEMRMFRWMCRQTRKDKIGNKDIWSKVGITVVVDKMREARL 138


>UniRef50_UPI0000E490F6 Cluster: PREDICTED: similar to
            endonuclease-reverse transcriptase; n=5;
            Strongylocentrotus purpuratus|Rep: PREDICTED: similar to
            endonuclease-reverse transcriptase - Strongylocentrotus
            purpuratus
          Length = 1030

 Score = 38.3 bits (85), Expect = 0.11
 Identities = 19/49 (38%), Positives = 33/49 (67%), Gaps = 1/49 (2%)
 Frame = +2

Query: 344  EMRMLRWMCGVTRMDKIKNEYSRGSAKV-APVINKLKSGRLAWCGYVMR 487
            EM  LR + GVTR+DKI+N + + S  +   V++K+ + R+ + G+V+R
Sbjct: 907  EMACLRRIMGVTRLDKIRNTHIKESLNLDQDVMDKVSTKRIKYFGHVLR 955


>UniRef50_Q4Z066 Cluster: Putative uncharacterized protein; n=1;
           Plasmodium berghei|Rep: Putative uncharacterized protein
           - Plasmodium berghei
          Length = 185

 Score = 37.1 bits (82), Expect = 0.26
 Identities = 29/95 (30%), Positives = 44/95 (46%), Gaps = 2/95 (2%)
 Frame = -3

Query: 325 FMLYLLLTELIIVPKNNIYLIEISKTGSYYRIEIEIF*CAIP*IGSLSFVTYTYVSCVRF 146
           F  Y+   ++ I   NN+  I      +Y  + +  F CAIP   S     +  V  ++F
Sbjct: 62  FNKYINDVDIYIYYNNNLNFIHDRIFETYIFLFLNKFICAIPFFDSKDINKWKIV-ILKF 120

Query: 145 LKKHYNQISLRRFNLNIHSDKIHYQ--LHVFFFFF 47
           LK  ++ ISL   N  I  D I Y   L+ F+FF+
Sbjct: 121 LKNLFHNISLSYHNNYIIQDYIFYSFLLNSFYFFY 155


>UniRef50_UPI0000E4958A Cluster: PREDICTED: similar to
           endonuclease-reverse transcriptase, partial; n=7;
           Strongylocentrotus purpuratus|Rep: PREDICTED: similar to
           endonuclease-reverse transcriptase, partial -
           Strongylocentrotus purpuratus
          Length = 787

 Score = 35.9 bits (79), Expect = 0.60
 Identities = 19/49 (38%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
 Frame = +2

Query: 344 EMRMLRWMCGVTRMDKIKNEYSRGSAKV-APVINKLKSGRLAWCGYVMR 487
           EMR LR + GV  MDKI+NE  R    + + +  ++    L W G+V+R
Sbjct: 407 EMRCLRTILGVHLMDKIRNEEIRQRLNIPSTICEEITKRCLKWFGHVLR 455


>UniRef50_Q6ZRM1 Cluster: CDNA FLJ46255 fis, clone TESTI4023172;
           n=1; Homo sapiens|Rep: CDNA FLJ46255 fis, clone
           TESTI4023172 - Homo sapiens (Human)
          Length = 377

 Score = 35.9 bits (79), Expect = 0.60
 Identities = 22/72 (30%), Positives = 31/72 (43%)
 Frame = -2

Query: 536 HYHIHTSPSHMHLSPLYASHIHTTLTVRSLIC*LPGLLSHFLCYTHSLSCPFLSLHTSIS 357
           H H+HT P+H   +P   SH HT L + +  C      + +  + H+L C     H    
Sbjct: 204 HTHVHT-PAH---TPHTRSHNHTCLYLLTHACTNTPAYTSYTAHPHTLHCSPSHTHAPAH 259

Query: 356 TFASLPHALLFH 321
           T    PH  L H
Sbjct: 260 THMLTPHHSLTH 271


>UniRef50_A7S7P6 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 211

 Score = 35.1 bits (77), Expect = 1.1
 Identities = 26/80 (32%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
 Frame = -2

Query: 542 TLHYHI-HTSPSHMHLSPLYASHI-HTTLTVRSLIC*LPGLLSHFLCYTHSLSCPFLSLH 369
           TL+ H  HT  +H HLS  + +H  HT  T   LI  +   LS+ L YT +   P    H
Sbjct: 28  TLYTHTSHTPCTHTHLSYTFHTHTSHTPCTHAPLIHLVHTYLSYTL-YTRTSHTPCTHTH 86

Query: 368 TSISTFASLPHALLFHALLI 309
            + + +    H    HA L+
Sbjct: 87  LTYTLYTRTSHTPCTHAPLV 106


>UniRef50_Q7NBW8 Cluster: Putative uncharacterized protein; n=1;
           Mycoplasma gallisepticum|Rep: Putative uncharacterized
           protein - Mycoplasma gallisepticum
          Length = 177

 Score = 34.7 bits (76), Expect = 1.4
 Identities = 21/68 (30%), Positives = 33/68 (48%)
 Frame = -3

Query: 436 YRGYFRTSSAILILYLVHSCHSTHPSQHSHLCRMHSSFMLYLLLTELIIVPKNNIYLIEI 257
           Y+GYF    A     + +S  +    Q  HL ++H    +  +L  L+IVPK   Y+I I
Sbjct: 64  YKGYFFDFDAKQSSKINYSLKNIKQHQLDHLRKIHEQGGIAFIL--LLIVPKEEFYMIPI 121

Query: 256 SKTGSYYR 233
            K  S+ +
Sbjct: 122 KKIDSWLK 129


>UniRef50_O96121 Cluster: Putative uncharacterized protein PFB0080c;
           n=1; Plasmodium falciparum 3D7|Rep: Putative
           uncharacterized protein PFB0080c - Plasmodium falciparum
           (isolate 3D7)
          Length = 485

 Score = 34.3 bits (75), Expect = 1.8
 Identities = 18/45 (40%), Positives = 25/45 (55%)
 Frame = -2

Query: 137 ALQPNFTTKIQFKYTFRQNTLSITRVLFFFFVYHKSNISQTSSLF 3
           A + N +T IQFK TF++N  S    L  FF ++K N  + S  F
Sbjct: 72  AFEGNLSTNIQFKNTFKRNLSSKEPSLDDFFKFYKKNSYKLSESF 116


>UniRef50_UPI00004DBC97 Cluster: UPI00004DBC97 related cluster; n=1;
           Xenopus tropicalis|Rep: UPI00004DBC97 UniRef100 entry -
           Xenopus tropicalis
          Length = 173

 Score = 33.9 bits (74), Expect = 2.4
 Identities = 24/78 (30%), Positives = 29/78 (37%), Gaps = 7/78 (8%)
 Frame = -2

Query: 536 HYHIHTSP-SHMHLSPLYASHIHTTLTVRSLIC*LPGL------LSHFLCYTHSLSCPFL 378
           H HIH    +H+H   L   H+H        IC L  L       +H L   H  +CP  
Sbjct: 39  HLHIHACKHTHVHSHTLAHLHLHAYTRAHLQICTLTHLHLRAYTFTHLLTLAHLQACPLT 98

Query: 377 SLHTSISTFASLPHALLF 324
             HT   T   L H   F
Sbjct: 99  HSHTYALTLVHL-HTYTF 115


>UniRef50_A4KWG0 Cluster: Reverse transcriptase; n=3; Ostrinia
           nubilalis|Rep: Reverse transcriptase - Ostrinia
           nubilalis (European corn borer)
          Length = 497

 Score = 33.5 bits (73), Expect = 3.2
 Identities = 15/54 (27%), Positives = 27/54 (50%)
 Frame = +2

Query: 326 RGVHAAEMRMLRWMCGVTRMDKIKNEYSRGSAKVAPVINKLKSGRLAWCGYVMR 487
           R +   +  M R M GV+  D+I+NE  R   +V  +  ++   +  W G++ R
Sbjct: 378 RRLKVTQRAMERAMLGVSLRDRIRNEEIRRRTRVTDIARRIAKIKWQWAGHIAR 431


>UniRef50_UPI0000ECB802 Cluster: UPI0000ECB802 related cluster; n=1;
           Gallus gallus|Rep: UPI0000ECB802 UniRef100 entry -
           Gallus gallus
          Length = 392

 Score = 33.1 bits (72), Expect = 4.3
 Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
 Frame = -2

Query: 527 IHTSPSHMHLSPLYA-SHIHTTLTVRSLIC*LPGLLSHFLCYTHSLSCPFLSLHT 366
           +H+   H  L+ L+A +H  T L V +  C L  +L+H     H+L+C +  LHT
Sbjct: 89  LHSVACHYMLARLHAVTHACTLLHVVTHACTLLHMLAHCCMPLHTLACHYTLLHT 143


>UniRef50_Q7R8T3 Cluster: Streptococcus pyogenes AMV156; n=6;
           Plasmodium (Vinckeia)|Rep: Streptococcus pyogenes AMV156
           - Plasmodium yoelii yoelii
          Length = 1006

 Score = 32.7 bits (71), Expect = 5.6
 Identities = 19/54 (35%), Positives = 30/54 (55%)
 Frame = +1

Query: 100 LN*IFVVKFGCSAFLKTSRSLHKYM*RMIMIQFMVLRIKIFRFRFCNRNLSCLF 261
           LN IF + FGCS++LK   SL +     I  +  +L + I  F + N+N+  +F
Sbjct: 511 LNFIFTIMFGCSSYLKEHYSLTEEEKNNIFFKDNILDLPI--FEYFNKNVIDIF 562


>UniRef50_A5E0Q3 Cluster: Putative uncharacterized protein; n=2;
           Saccharomycetaceae|Rep: Putative uncharacterized protein
           - Lodderomyces elongisporus (Yeast) (Saccharomyces
           elongisporus)
          Length = 1005

 Score = 32.7 bits (71), Expect = 5.6
 Identities = 17/62 (27%), Positives = 26/62 (41%)
 Frame = -2

Query: 536 HYHIHTSPSHMHLSPLYASHIHTTLTVRSLIC*LPGLLSHFLCYTHSLSCPFLSLHTSIS 357
           H+H H    H H+ PL  +H H+  +   ++       SH    TH    P +   T+ S
Sbjct: 45  HHHHHLHHHHHHIHPLNHTHSHSQASPTGVVDATSMNCSHHCHQTHQSPTPVIIQPTATS 104

Query: 356 TF 351
            F
Sbjct: 105 QF 106


>UniRef50_UPI000069E6CB Cluster: UPI000069E6CB related cluster; n=1;
           Xenopus tropicalis|Rep: UPI000069E6CB UniRef100 entry -
           Xenopus tropicalis
          Length = 160

 Score = 32.3 bits (70), Expect = 7.4
 Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 2/74 (2%)
 Frame = -2

Query: 530 HIHTSPSHMHLSPLYASHIHTTLTVRSLIC*LPGLLSHF--LCYTHSLSCPFLSLHTSIS 357
           H++T+ +H H+   Y +H+HT      L       L+HF  L YTH+LS    S HT   
Sbjct: 81  HVYTN-THKHIFFYYYTHLHTLFFFLFL-----HTLAHFYTLIYTHTLSHNASSTHTHTH 134

Query: 356 TFASLPHALLFHAL 315
            +  +   LL +A+
Sbjct: 135 IYIYIYLHLLANAM 148


>UniRef50_Q8IBB3 Cluster: Leucine-tRNA ligase; n=3; cellular
           organisms|Rep: Leucine-tRNA ligase - Plasmodium
           falciparum (isolate 3D7)
          Length = 1481

 Score = 32.3 bits (70), Expect = 7.4
 Identities = 16/47 (34%), Positives = 25/47 (53%)
 Frame = -2

Query: 158 LREVFKKALQPNFTTKIQFKYTFRQNTLSITRVLFFFFVYHKSNISQ 18
           ++E+  K  + N+  K  F +++ QN L +    FFF  YH  NI Q
Sbjct: 408 IKELNNKIQKYNYINKYDFFFSYHQNDLFLF-FSFFFNYYHNRNIDQ 453


>UniRef50_UPI0000D8F084 Cluster: UPI0000D8F084 related cluster; n=1;
           Danio rerio|Rep: UPI0000D8F084 UniRef100 entry - Danio
           rerio
          Length = 185

 Score = 31.9 bits (69), Expect = 9.8
 Identities = 15/30 (50%), Positives = 20/30 (66%)
 Frame = -1

Query: 477 YPHHANRPLFNLLITGATFALPLLYSFFIL 388
           +PH    PLF L I+G++F  P  YS+FIL
Sbjct: 151 FPHSV--PLFCLYISGSSFKSPSAYSYFIL 178


>UniRef50_Q5QZE5 Cluster: Uncharacterized conserved membrane
           protein; n=1; Idiomarina loihiensis|Rep: Uncharacterized
           conserved membrane protein - Idiomarina loihiensis
          Length = 119

 Score = 31.9 bits (69), Expect = 9.8
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -2

Query: 431 GLLSHFLCYTHSLSCPFLSLHTSISTFASLPHALLFHALLI 309
           G+    LC+ H L+ P L +    ++ AS+    LFH LL+
Sbjct: 6   GIFFSGLCFVHCLATPILLIFLGTNSLASVLETPLFHRLLL 46


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 515,277,144
Number of Sequences: 1657284
Number of extensions: 9674291
Number of successful extensions: 25286
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 24281
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25249
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 34989170748
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -