BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0240 (729 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 119 5e-28 SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 35 0.014 SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 29 0.51 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 29 0.90 SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 29 0.90 SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 27 2.1 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 27 2.1 SPAC20H4.02 |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.6 SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 27 3.6 SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 26 4.8 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 26 6.3 SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 26 6.3 SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 26 6.3 SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 25 8.4 >SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 119 bits (286), Expect = 5e-28 Identities = 61/156 (39%), Positives = 97/156 (62%), Gaps = 3/156 (1%) Frame = +1 Query: 64 KFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVTDIRRML- 240 K+DL+ KI Q+LDRHL+FPLLEFL+ ++T+D ELLQAK ++L TNM DYV ++ L Sbjct: 14 KYDLSQKIMQHLDRHLIFPLLEFLSLRQTHDPKELLQAKYDLLKDTNMTDYVANLWTNLH 73 Query: 241 --YPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMKTIETMRDPKTLINHLS 414 + +ED ++R VL EL EL++ V+ +L +++ D++ + +D + HL Sbjct: 74 GGHTDEDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALR--QDKGQNLQHL- 130 Query: 415 TNKEYEFKIEMIDSMYRLAKYRYECGNYVESASYLY 522 + Y E I +Y+ A+++Y CGNY ++ LY Sbjct: 131 -QEHYNITPERIAVLYKFAQFQYNCGNYGGASDLLY 165 Score = 26.6 bits (56), Expect = 3.6 Identities = 10/16 (62%), Positives = 11/16 (68%) Frame = +2 Query: 566 SVLWGKLASEILVQNW 613 S WGK ASEIL +W Sbjct: 179 SATWGKFASEILTVDW 194 Score = 25.4 bits (53), Expect = 8.4 Identities = 9/16 (56%), Positives = 14/16 (87%) Frame = +3 Query: 615 DGALDDLTKLREFIDN 662 DGA+++L KLRE +D+ Sbjct: 195 DGAMEELGKLREMVDS 210 >SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 796 Score = 34.7 bits (76), Expect = 0.014 Identities = 24/78 (30%), Positives = 37/78 (47%) Frame = -1 Query: 615 PQFWTKISLANLPQRTDKIILVSWRHHQLTKVKI*C*LHIISTFIAIFGQTIHAIDHFNF 436 P + + A L ++++ V W+HHQ C I + F IFGQ I AID ++ Sbjct: 315 PSNYLSPNRAELESTPEQLLEVPWQHHQ-------CHPLIKTHFFGIFGQKIDAIDFYSA 367 Query: 435 KLILLVCRKMVYKSFRIP 382 KL + + +SF P Sbjct: 368 KLYKISQQIENARSFDYP 385 >SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual Length = 2410 Score = 29.5 bits (63), Expect = 0.51 Identities = 11/44 (25%), Positives = 25/44 (56%) Frame = +1 Query: 367 TIETMRDPKTLINHLSTNKEYEFKIEMIDSMYRLAKYRYECGNY 498 T+ T+ D ++ HL+ +F + ++M++ ++YRY N+ Sbjct: 180 TVATLGDLIGMVGHLNDTSGVDFNLNEYNAMWKTSEYRYVDFNF 223 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 28.7 bits (61), Expect = 0.90 Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%) Frame = +1 Query: 79 FKIGQYLDRHLVFPLLEF-LAAKETYDQSELLQAKLEILSK 198 F I +YL +L+ P LEF L A E Q + A ++ SK Sbjct: 1218 FHISKYLSSNLLLPFLEFLLTASEAEAQIPVTDASQKVSSK 1258 >SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 793 Score = 28.7 bits (61), Expect = 0.90 Identities = 14/59 (23%), Positives = 29/59 (49%), Gaps = 5/59 (8%) Frame = -1 Query: 489 TFIAIFGQTIHAIDHFNFKLILLVCRKMVYKSFRIPH-----GFNCFHYIISLHESQYW 328 TF+ +G I+ D +N L +VY ++ + F+ + ++S+++SQ W Sbjct: 83 TFLVKYGDLINIQDPYNLNKTLFSVSDLVYNGIQLDYDSYSISFDAKYVLVSVNKSQRW 141 >SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein intermediate chain Dic1|Schizosaccharomyces pombe|chr 2|||Manual Length = 544 Score = 27.5 bits (58), Expect = 2.1 Identities = 16/63 (25%), Positives = 29/63 (46%) Frame = +1 Query: 178 KLEILSKTNMIDYVTDIRRMLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDD 357 K+ + S N I Y + +L P+E ++ RG+ + Q +++ E VL+ Sbjct: 188 KITLNSVVNSISYSSFFEELLITSYAKPKEALRTRGLAIVWNQRWKNSPESVLKARSEIT 247 Query: 358 VMK 366 V K Sbjct: 248 VCK 250 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 27.5 bits (58), Expect = 2.1 Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 3/44 (6%) Frame = +1 Query: 352 DDVMKTIETMRDPKTLINHLSTNKE---YEFKIEMIDSMYRLAK 474 +DV+K + T+ PK +I L ++ +EF I+ ID ++ +K Sbjct: 3764 NDVLKKLITILSPKVIITKLDFERDTFVHEFGIDSIDLIWLASK 3807 >SPAC20H4.02 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 26.6 bits (56), Expect = 3.6 Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 6/56 (10%) Frame = +3 Query: 579 VNWLAKFWSKIGDGALDDLTK--LRE----FIDNGGAGSTASNMQALQQRTLAGSL 728 VN L + ++ LD LT+ +RE +IDNGG S+A + + LAG L Sbjct: 150 VNNLRSQFHRLRGTNLDSLTEDAIREAEDDWIDNGGQNSSADELDMSYETLLAGVL 205 >SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 26.6 bits (56), Expect = 3.6 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = -2 Query: 683 GSGTGSTVIDELSKFG*IIERAIP 612 GSGTGSTV L+ F + R +P Sbjct: 272 GSGTGSTVFQVLNAFSKAVGRDLP 295 >SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1388 Score = 26.2 bits (55), Expect = 4.8 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = +3 Query: 576 GVNWLAKFWSKIGDGALDD 632 GVNW+A W K +G L D Sbjct: 382 GVNWMAYLWHKNENGILAD 400 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 25.8 bits (54), Expect = 6.3 Identities = 10/40 (25%), Positives = 22/40 (55%) Frame = +1 Query: 325 EPVLRLMQRDDVMKTIETMRDPKTLINHLSTNKEYEFKIE 444 +PV+R+ + I + + P+ L H S K+Y++ ++ Sbjct: 1947 KPVIRISHFHHTFEVISSKQRPRRLTIHGSDGKDYQYVLK 1986 >SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 781 Score = 25.8 bits (54), Expect = 6.3 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = -3 Query: 553 CQLATSPVDKSKDMMLTPHNFHIHSDIW 470 C++ V KS D+ + H F ++SD W Sbjct: 506 CRMEHPNVVKSYDLCIDSHIFPLYSDTW 533 >SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 661 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/41 (31%), Positives = 22/41 (53%), Gaps = 1/41 (2%) Frame = +1 Query: 217 VTDIRRMLYPEEDTPEEIIQRRGVVLSELQ-ELQDAVEPVL 336 VTDI+ +L + DTP ++ G + L+ +EP+L Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTTPASVSLRQMIEPLL 573 >SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 25.4 bits (53), Expect = 8.4 Identities = 12/44 (27%), Positives = 21/44 (47%) Frame = +1 Query: 142 KETYDQSELLQAKLEILSKTNMIDYVTDIRRMLYPEEDTPEEII 273 ++ YD SE+L ++E+ S Y + P E T E ++ Sbjct: 163 QKLYDSSEVLDDRIELFSALTCRKYNISDEDLANPSELTQEPVV 206 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,997,031 Number of Sequences: 5004 Number of extensions: 61356 Number of successful extensions: 190 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 188 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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