Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0240
(729 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g57290.1 68416.m06377 eukaryotic translation initiation facto... 126 2e-29
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 31 0.78
At4g12610.1 68417.m01987 transcription initiation factor IIF alp... 28 7.3
At3g61140.1 68416.m06842 COP9 signalosome complex subunit 1 / CS... 28 7.3
At3g58340.1 68416.m06503 meprin and TRAF homology domain-contain... 28 7.3
At1g26090.1 68414.m03182 hypothetical protein 28 7.3
At5g62810.1 68418.m07884 peroxisomal protein (PEX14) identical t... 27 9.6
At2g46810.1 68415.m05841 basic helix-loop-helix (bHLH) family pr... 27 9.6
At1g25510.1 68414.m03168 aspartyl protease family protein contai... 27 9.6
>At3g57290.1 68416.m06377 eukaryotic translation initiation factor
3E / eIF3e (TIF3E1) identical to eukaryotic initiation
factor 3E subunit [Arabidopsis thaliana]
gi|12407658|gb|AAG53613
Length = 441
Score = 126 bits (303), Expect = 2e-29
Identities = 58/168 (34%), Positives = 105/168 (62%)
Frame = +1
Query: 55 SYSKFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVTDIRR 234
S +DLT I LDRHLVFP+ EFL ++ Y ++L++K+++L++TNM+DY DI +
Sbjct: 4 SKQNYDLTPLIAPNLDRHLVFPIFEFLQERQLYPDEQILKSKIQLLNQTNMVDYAMDIHK 63
Query: 235 MLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMKTIETMRDPKTLINHLS 414
LY ED P+E+++RR V++ L+ L++A P++ + + ++ +R K N
Sbjct: 64 SLYHTEDAPQEMVERRTEVVARLKSLEEAAAPLVSFLLNPN---AVQELRADKQ-YNLQM 119
Query: 415 TNKEYEFKIEMIDSMYRLAKYRYECGNYVESASYLYFCQLVMSPTDKN 558
+ Y+ + I+++Y+ AK+++ECGNY +A YLY + + S +++
Sbjct: 120 LKERYQIGPDQIEALYQYAKFQFECGNYSGAADYLYQYRTLCSNLERS 167
Score = 38.3 bits (85), Expect = 0.005
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = +2
Query: 539 CRQLTRIILSVLWGKLASEILVQNW 613
C L R LS LWGKLASEIL+QNW
Sbjct: 161 CSNLERS-LSALWGKLASEILMQNW 184
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 31.1 bits (67), Expect = 0.78
Identities = 28/115 (24%), Positives = 54/115 (46%), Gaps = 5/115 (4%)
Frame = +1
Query: 139 AKETYDQSELLQAKLEILSKT-----NMIDYVTDIRRMLYPEEDTPEEIIQRRGVVLSEL 303
A+E ++ ++++ K++ +K NM V D ++ D +E ++ GV+ L
Sbjct: 567 AEEFAEEDKIMKEKIDARNKLETYVYNMKSTVADKEKLAKKISDEDKEKME--GVLKEAL 624
Query: 304 QELQDAVEPVLRLMQRDDVMKTIETMRDPKTLINHLSTNKEYEFKIEMIDSMYRL 468
+ L++ V D+ +K +E + DP + T E E +EMI YR+
Sbjct: 625 EWLEENVNAEKE--DYDEKLKEVELVCDPVIKSVYEKTEGENEDTMEMITMSYRI 677
>At4g12610.1 68417.m01987 transcription initiation factor IIF alpha
subunit (TFIIF-alpha) family protein low similarity to
SP|Q05913 Transcription initiation factor IIF, alpha
subunit (TFIIF-alpha) (Transcription factor 5, large
chain) (TF5A) {Drosophila melanogaster}; contains Pfam
profile PF05793: Transcription initiation factor IIF,
alpha subunit (TFIIF-alpha)
Length = 543
Score = 27.9 bits (59), Expect = 7.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +1
Query: 481 YECGNYVESASYLYFCQLVMSPTDKNYFVG 570
+ECG V Y + +PTDKNYF+G
Sbjct: 46 HECGTVVTRLIREYNVRAA-APTDKNYFIG 74
>At3g61140.1 68416.m06842 COP9 signalosome complex subunit 1 / CSN
complex subunit 1 (CSN1) / COP11 protein (COP11) / FUSCA
protein (FUS6) FUSCA6, COP11, CSN1; identical to FUS6
GI:432446, SP:P45432 from [Arabidopsis thaliana];
contains Pfam profile PF01399: PCI domain; identical to
cDNA CSN complex subunit 1 (CSN1) GI:18056652
Length = 441
Score = 27.9 bits (59), Expect = 7.3
Identities = 11/36 (30%), Positives = 23/36 (63%)
Frame = +1
Query: 133 LAAKETYDQSELLQAKLEILSKTNMIDYVTDIRRML 240
L A ++D+SEL Q ++ ++ N ++ V D+R ++
Sbjct: 264 LCALASFDRSELKQKVIDNINFRNFLELVPDVRELI 299
>At3g58340.1 68416.m06503 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 325
Score = 27.9 bits (59), Expect = 7.3
Identities = 22/100 (22%), Positives = 50/100 (50%), Gaps = 6/100 (6%)
Frame = +1
Query: 70 DLTFKIGQYL--DRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVTDIRRMLY 243
+L K G +L + ++ +EFL T D+SE + ++++KT + ++ ++
Sbjct: 111 ELNAKDGGFLVNGQVMIVAEVEFLEVIGTLDESEEIIKSSDLINKTQ--EVAQQVKEIIQ 168
Query: 244 PEE--DTPEEIIQ--RRGVVLSELQELQDAVEPVLRLMQR 351
P + + +E+ Q + + ++ Q L VE V R+ ++
Sbjct: 169 PNDLINKTQEVAQQVKESIDVNGFQVLPSQVESVRRIFKK 208
>At1g26090.1 68414.m03182 hypothetical protein
Length = 455
Score = 27.9 bits (59), Expect = 7.3
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 3/41 (7%)
Frame = +3
Query: 609 IGDGALDD---LTKLREFIDNGGAGSTASNMQALQQRTLAG 722
+ D A D LTK F+ GG+G T + + A Q LAG
Sbjct: 46 VNDTAADSSQKLTKFVTFLGKGGSGKTTAAVFAAQHYALAG 86
>At5g62810.1 68418.m07884 peroxisomal protein (PEX14) identical to
PEX14 [Arabidopsis thaliana] GI:11094252; contains Pfam
profile PF04695: Peroxisomal membrane anchor protein
(Pex14p) conserved region; supporting cDNA
gi|11094253|dbj|AB037539.1|
Length = 507
Score = 27.5 bits (58), Expect = 9.6
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 618 GALDDLTKLREFIDNGGAGSTASNMQALQQRT 713
G D+L K+ +F ++GG GS + +Q+ T
Sbjct: 466 GVSDELQKITKFSESGGDGSGGIKIAEIQEET 497
>At2g46810.1 68415.m05841 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 371
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/85 (22%), Positives = 38/85 (44%), Gaps = 3/85 (3%)
Frame = +1
Query: 154 DQSELLQAKLEILSKTNMIDYVTDIRRMLYPEEDTPEE---IIQRRGVVLSELQELQDAV 324
+QS L +++ + +D ++RR L +E + ++ LS LQD
Sbjct: 8 NQSFKLHQQVQCKDEIFCLDQKVNVRRSLQVQETVEDHQSFALEEEEQQLSTPSLLQDTT 67
Query: 325 EPVLRLMQRDDVMKTIETMRDPKTL 399
P L+++Q+ + + +DP L
Sbjct: 68 IPFLQMLQQSEDPSPFLSFKDPSFL 92
>At1g25510.1 68414.m03168 aspartyl protease family protein contains
Pfam domain, PF00026: eukaryotic aspartyl protease
Length = 483
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -1
Query: 282 SPLYNFFRSVFFRIEHSSDVSNIIYHVS 199
SP Y+FF +FF HSS S I+ S
Sbjct: 2 SPNYSFFFFIFFLTSHSSVFSRILPETS 29
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,481,320
Number of Sequences: 28952
Number of extensions: 312554
Number of successful extensions: 896
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 865
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 895
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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