Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0235
(704 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g19006.1 68417.m02801 26S proteasome regulatory subunit, puta... 120 1e-27
At5g45620.2 68418.m05607 26S proteasome regulatory subunit, puta... 115 3e-26
At5g45620.1 68418.m05608 26S proteasome regulatory subunit, puta... 115 3e-26
At2g33090.1 68415.m04057 expressed protein ; expression supporte... 29 3.0
At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative / phos... 29 4.0
At2g01560.1 68415.m00080 hypothetical protein 29 4.0
At2g20800.1 68415.m02446 pyridine nucleotide-disulphide oxidored... 28 5.2
At2g26900.1 68415.m03227 bile acid:sodium symporter family prote... 27 9.2
At1g58050.1 68414.m06579 helicase domain-containing protein cont... 27 9.2
At1g05460.1 68414.m00555 RNA helicase SDE3 (SDE3) identical to R... 27 9.2
At1g03860.3 68414.m00370 prohibitin, putative similar to SP|P241... 27 9.2
At1g03860.2 68414.m00368 prohibitin, putative similar to SP|P241... 27 9.2
At1g03860.1 68414.m00369 prohibitin, putative similar to SP|P241... 27 9.2
>At4g19006.1 68417.m02801 26S proteasome regulatory subunit,
putative (RPN9) similar to 26S proteasome subunit p40.5
[Homo sapiens] gi|3618343|dbj|BAA33214
Length = 386
Score = 120 bits (288), Expect = 1e-27
Identities = 67/180 (37%), Positives = 103/180 (57%), Gaps = 5/180 (2%)
Frame = +3
Query: 39 FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTT 218
+L ++ + P L + L +LY KKLWHQLTLKL++F+ Q GD LIQLYNNF+T
Sbjct: 6 YLESQKNAHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALSVFQAGDALIQLYNNFITD 65
Query: 219 FESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVKMNDEAL--ALCKVLQGQIYIE 392
FE++IN L L + QY K AV++LE V K+K E+ ++ QI +
Sbjct: 66 FETRINLLKLAHFAVVVSRQYPEKEAAVSYLEGVIEKLKATKESRINEPISYIETQIALF 125
Query: 393 QLNDYDAAE--KIIEHLESTLEDADGVTP-VHGRFYKLASEYYRVRGPTGRYYRAALRYV 563
+L D E KI++ +S L+ + P V+ F+ ++S+Y++VR +Y+ AL Y+
Sbjct: 126 KLEQGDQKECKKILDDGKSLLDSMTDIDPSVYANFFWVSSQYHKVRQEFSEFYKNALLYL 185
Score = 32.3 bits (70), Expect = 0.32
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2
Query: 635 SGVLAPDVYDLGELVAHPILHSL 703
S +L ++Y+ GEL+AHPIL SL
Sbjct: 208 SALLGENIYNFGELLAHPILKSL 230
>At5g45620.2 68418.m05607 26S proteasome regulatory subunit,
putative (RPN9) contains similarity to 26S proteasome
subunit p40.5 GI:3618343 from [Homo sapiens]
Length = 350
Score = 115 bits (277), Expect = 3e-26
Identities = 62/180 (34%), Positives = 100/180 (55%), Gaps = 5/180 (2%)
Frame = +3
Query: 39 FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTT 218
+L + + P L + L +LY KKLWHQLTLKL++F+ Q GD LIQ Y+NF+T
Sbjct: 6 YLESLKNTHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNFITD 65
Query: 219 FESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVKMNDEALALCKVL----QGQIY 386
FE+KIN L L + QY K AV++LE V K++ E ++ Q ++
Sbjct: 66 FETKINLLKLAHFAVVVSRQYSEKEAAVSYLESVIEKLRATKEPRITEPIIYIETQKALF 125
Query: 387 IEQLNDYDAAEKIIEHLESTLEDADGVTP-VHGRFYKLASEYYRVRGPTGRYYRAALRYV 563
+ D +KI++ +S+L+ + P V+ FY ++S+Y++ R +Y++AL Y+
Sbjct: 126 KLEQGDQKECKKILDDGKSSLDSMTDIDPSVYANFYWVSSQYHKCRQEFSDFYKSALLYL 185
Score = 32.3 bits (70), Expect = 0.32
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2
Query: 635 SGVLAPDVYDLGELVAHPILHSL 703
S +L ++Y+ GEL+AHPIL SL
Sbjct: 208 SALLGENIYNFGELLAHPILKSL 230
>At5g45620.1 68418.m05608 26S proteasome regulatory subunit,
putative (RPN9) contains similarity to 26S proteasome
subunit p40.5 GI:3618343 from [Homo sapiens]
Length = 386
Score = 115 bits (277), Expect = 3e-26
Identities = 62/180 (34%), Positives = 100/180 (55%), Gaps = 5/180 (2%)
Frame = +3
Query: 39 FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTT 218
+L + + P L + L +LY KKLWHQLTLKL++F+ Q GD LIQ Y+NF+T
Sbjct: 6 YLESLKNTHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNFITD 65
Query: 219 FESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVKMNDEALALCKVL----QGQIY 386
FE+KIN L L + QY K AV++LE V K++ E ++ Q ++
Sbjct: 66 FETKINLLKLAHFAVVVSRQYSEKEAAVSYLESVIEKLRATKEPRITEPIIYIETQKALF 125
Query: 387 IEQLNDYDAAEKIIEHLESTLEDADGVTP-VHGRFYKLASEYYRVRGPTGRYYRAALRYV 563
+ D +KI++ +S+L+ + P V+ FY ++S+Y++ R +Y++AL Y+
Sbjct: 126 KLEQGDQKECKKILDDGKSSLDSMTDIDPSVYANFYWVSSQYHKCRQEFSDFYKSALLYL 185
Score = 32.3 bits (70), Expect = 0.32
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2
Query: 635 SGVLAPDVYDLGELVAHPILHSL 703
S +L ++Y+ GEL+AHPIL SL
Sbjct: 208 SALLGENIYNFGELLAHPILKSL 230
>At2g33090.1 68415.m04057 expressed protein ; expression supported
by MPSS
Length = 248
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/65 (27%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = -1
Query: 641 LQKRASLKAI--SARSVAGRASPPVGAPNVPKRCAVVTASGTTDSVIFRG*LIKSSVNWS 468
L+KR K++ ++R + + PPV + PK C V+T + T +F + K S +++
Sbjct: 111 LKKRGLSKSVPETSRLIKLKKQPPVKVSDKPKSCPVLTKNDTDTLELFE--IAKKSADFA 168
Query: 467 HTISI 453
+ I
Sbjct: 169 NAKGI 173
>At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative
phosphatidylinositol-phosphatidylcholine transfer
protein SEC14, Yarrowia lipolytica, PIR2:S43745;
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 554
Score = 28.7 bits (61), Expect = 4.0
Identities = 39/142 (27%), Positives = 60/142 (42%), Gaps = 9/142 (6%)
Frame = +3
Query: 63 EPALAADW-AKLEELYNKKLWHQLTLKLQEFVKNPALQRG---DNLIQLYNNFLTTFE-S 227
+ A AADW KL NK + LK NP ++G +++ L + +
Sbjct: 387 DAAAAADWPTKL----NKAEKNPTDLKDVYSAVNPLERKGYLYGSVMALLMGIVGVMRLT 442
Query: 228 KINPLSLVEIIAHIVEQYVNKRDAVTFLEKVE--TKVKMNDEALALCKVLQGQ--IYIEQ 395
K P L E + E +D VT + K E VK + CK ++ Q Y+E+
Sbjct: 443 KNMPRRLTEANVYSREGSAVYQDGVTVMSKQEYIAMVKKITDLEEKCKSMEAQAAFYMER 502
Query: 396 LNDYDAAEKIIEHLESTLEDAD 461
DAA + I+ LE L + +
Sbjct: 503 EKTLDAALRRIDQLELQLSETN 524
>At2g01560.1 68415.m00080 hypothetical protein
Length = 277
Score = 28.7 bits (61), Expect = 4.0
Identities = 21/81 (25%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Frame = +3
Query: 285 NKRDAVTFLEKVETKVKMNDEALALCKVLQGQIYIEQLND-YDAAEKIIEHLESTLEDAD 461
N D +EKV T+ D A + +I ++Q++ E IIE + L +
Sbjct: 156 NSSDTPIPVEKVSTRGTSADVERAEVPIADAEIILKQISTRMRLLESIIEQVHKLLSNVL 215
Query: 462 GVTPVHGRFYKLASEYYRVRG 524
G P + K + +VRG
Sbjct: 216 GPPPSAAKALKKQRSWKQVRG 236
>At2g20800.1 68415.m02446 pyridine nucleotide-disulphide
oxidoreductase family protein similar to GI:3718005
alternative NADH-dehydrogenase {Yarrowia lipolytica} ;
contains Pfam profile PF00070: Pyridine
nucleotide-disulphide oxidoreductase
Length = 582
Score = 28.3 bits (60), Expect = 5.2
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +3
Query: 138 KLQEFVKNPALQRGDNLIQLYNNFLTTF 221
K+QEF K L+ GD+++ +++ +T F
Sbjct: 259 KVQEFTKITLLEAGDHILNMFDKRITAF 286
>At2g26900.1 68415.m03227 bile acid:sodium symporter family protein
low similarity to SP|Q12908 Ileal sodium/bile acid
cotransporter {Homo sapiens}; contains Pfam profile
PF01758: Sodium Bile acid symporter family
Length = 409
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = -2
Query: 346 SSFILTFVSTFSRNVTASLLLTYCSTI*AMIST 248
+S + T++S NV S+L+T CSTI A+I T
Sbjct: 204 ASNVATYIS--KGNVALSVLMTTCSTIGAIIMT 234
>At1g58050.1 68414.m06579 helicase domain-containing protein contains
similarity to SP|P24785 Dosage compensation regulator
(Male-less protein) (No action potential protein)
{Drosophila melanogaster}; contains Pfam profiles
PF04408: Helicase associated domain (HA2), PF00271:
Helicase conserved C-terminal domain
Length = 1417
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 6/50 (12%)
Frame = +3
Query: 402 DYDAAEKIIEHLESTLEDA------DGVTPVHGRFYKLASEYYRVRGPTG 533
DYD E++I H++ T E+ G++ ++ +LA+ YR RG +G
Sbjct: 865 DYDLLEELICHIDDTCEEGAILVFLPGMSEINMLLNRLAAS-YRFRGASG 913
>At1g05460.1 68414.m00555 RNA helicase SDE3 (SDE3) identical to RNA
helicase SDE3 [Arabidopsis thaliana] GI:13811296
Length = 1002
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/75 (21%), Positives = 32/75 (42%), Gaps = 1/75 (1%)
Frame = +3
Query: 141 LQEFVKNPALQRGD-NLIQLYNNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEK 317
++ N +Q D +I Y + + ++ L + E+ VEQ+ + V +
Sbjct: 695 IKRLTANDCVQEEDIGVITPYRQQVMKIKEVLDRLDMTEVKVGSVEQFQGQEKQVIIIST 754
Query: 318 VETKVKMNDEALALC 362
V + +K N+ A C
Sbjct: 755 VRSTIKHNEFDRAYC 769
>At1g03860.3 68414.m00370 prohibitin, putative similar to SP|P24142
Prohibitin (B-cell receptor associated protein 32) (BAP
32) {Rattus norvegicus}; contains Pfam profile PF01145:
SPFH domain / Band 7 family
Length = 286
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +3
Query: 177 GDNLIQLYNNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVK 335
GD L Q+Y + ++ P + E + +V QY N +T E V +++
Sbjct: 109 GDRLPQIYRTLGENYSERVLPSIIHETLKAVVAQY-NASQLITQREAVSREIR 160
>At1g03860.2 68414.m00368 prohibitin, putative similar to SP|P24142
Prohibitin (B-cell receptor associated protein 32) (BAP
32) {Rattus norvegicus}; contains Pfam profile PF01145:
SPFH domain / Band 7 family
Length = 221
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +3
Query: 177 GDNLIQLYNNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVK 335
GD L Q+Y + ++ P + E + +V QY N +T E V +++
Sbjct: 44 GDRLPQIYRTLGENYSERVLPSIIHETLKAVVAQY-NASQLITQREAVSREIR 95
>At1g03860.1 68414.m00369 prohibitin, putative similar to SP|P24142
Prohibitin (B-cell receptor associated protein 32) (BAP
32) {Rattus norvegicus}; contains Pfam profile PF01145:
SPFH domain / Band 7 family
Length = 286
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +3
Query: 177 GDNLIQLYNNFLTTFESKINPLSLVEIIAHIVEQYVNKRDAVTFLEKVETKVK 335
GD L Q+Y + ++ P + E + +V QY N +T E V +++
Sbjct: 109 GDRLPQIYRTLGENYSERVLPSIIHETLKAVVAQY-NASQLITQREAVSREIR 160
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,048,810
Number of Sequences: 28952
Number of extensions: 318386
Number of successful extensions: 806
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 787
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 803
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -