Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0229
         (720 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz...   101   8e-23
SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Sch...    96   4e-21
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c...    31   0.22 
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra...    29   0.67 
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4...    29   0.67 
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    29   0.67 
SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr 1|...    28   1.5  
SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pom...    27   2.0  
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha...    27   2.7  
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic...    26   6.2  
SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces ...    25   8.2  

>SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 440

 Score =  101 bits (243), Expect = 8e-23
 Identities = 52/190 (27%), Positives = 96/190 (50%), Gaps = 6/190 (3%)
 Frame = +2

Query: 20  RKFNIDTTPKLLFSRGPLVELLISSNIARYAEFRCVTRVLTWLNDQLMPVPCSRADVFAT 199
           R + +D  PK L + G L  +LI +++ RY EF+ +     + + ++  VP +  +   +
Sbjct: 69  RDWCVDLVPKFLMANGDLTNILIYTDVTRYIEFKQIAGSYVYRDGRIAKVPGNEMEALKS 128

Query: 200 EAVGIVEKRMLMKMLTSIVGYSEEEMNNEFKDWN------NKTLKEYLTHKGLTPNLIHY 361
             + + EKR   K L  +  Y E++ +  +KD N          K++    G    + H 
Sbjct: 129 PLMSLFEKRRAKKFLEWVNNYREDDPST-YKDINIDRDSMESVFKKFGLQSGTQDFIGHA 187

Query: 362 ILYAIAGGNNSLPCLEGVKECKKFLMSLGRYGNTPFLWPMYGSGELPQCFCRLCAVFGGV 541
           +   +       P  E  +    +  S+ ++G +P+++P+YG GELPQ F RL A++GG 
Sbjct: 188 MALYLDDAYLKKPARETRERILLYASSIAKFGKSPYIYPLYGLGELPQGFARLSAIYGGT 247

Query: 542 YCLNRPIDKV 571
           Y LN+P+D++
Sbjct: 248 YMLNQPVDEI 257


>SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 459

 Score = 96.3 bits (229), Expect = 4e-21
 Identities = 61/194 (31%), Positives = 106/194 (54%), Gaps = 7/194 (3%)
 Frame = +2

Query: 44  PKLLFSRGPLVELLISSNIARYAEFRCVT--RVLTWLNDQLMPVPCSRADVFATEAVGIV 217
           P+ +F+   LV+LL  + I +Y   +     R+LT  N++ + VP SRAD+F  + + + 
Sbjct: 104 PQEIFASSELVKLLSETKIYKYLLLKPARSFRLLT-SNEEWIKVPESRADIFNNKNLSLA 162

Query: 218 EKRMLMKMLTSIVGYSEEEMNNEFKDWNNKTLKEYLTHK-GLTPNLIHYILYAIAGG-NN 391
            KR++M+ +  +   ++E+  N  K+W +K   ++L     L+  +   I+Y +    + 
Sbjct: 163 SKRIVMRFMKFVSNIADEQNQNLVKEWESKPFYKFLEEVFQLSGAIEESIIYGLCQSLSK 222

Query: 392 SLPCLEGVKECKKFLMSLGRYGNTPFLWPMYGSG-ELPQCFCRLCAVFGGVYCLNRPIDK 568
            +P  + +    K+  S G YG+  +L  MYG+G EL Q FCR  AV GG + L + IDK
Sbjct: 223 DIPTKDALDTVLKYFHSFGMYGDYSYLLAMYGTGSELCQGFCRSSAVMGGTFMLGQAIDK 282

Query: 569 V-ETKTV-DEGKTV 604
           + E+K V  +G T+
Sbjct: 283 IDESKIVLKDGSTL 296


>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1687

 Score = 30.7 bits (66), Expect = 0.22
 Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
 Frame = +2

Query: 224  RMLMKMLTSIVGYSEEEMNNEFKDWNNKTLKEYLTHKGLT--PNL-IHYILY 370
            RML+++  ++  Y E     +F D   KTL   L H G +  PNL + ++ Y
Sbjct: 1231 RMLLEVAKNVNTYCESRQQKDFIDLMGKTLSSVLFHAGKSSDPNLTVDFLAY 1282


>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
           3-phosphatidyltransferase Pis1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 251

 Score = 29.1 bits (62), Expect = 0.67
 Identities = 16/51 (31%), Positives = 27/51 (52%)
 Frame = +2

Query: 479 MYGSGELPQCFCRLCAVFGGVYCLNRPIDKVETKTVDEGKTVVTIDSKSKK 631
           +Y +GELP  +  L AV  G  CL + I  V  + V+    +V +D + ++
Sbjct: 196 IYSTGELPLSYPTLLAVLCGPICLAKQIINV-VQLVNAANALVKMDVEQRR 245


>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
           Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 684

 Score = 29.1 bits (62), Expect = 0.67
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +3

Query: 594 EKPSSPSIASRRSLTAITWSSASTNAPRS 680
           E+PS+P +   R+LT IT S      PRS
Sbjct: 640 ERPSTPPVEIERALTPITTSPQKLKLPRS 668


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 29.1 bits (62), Expect = 0.67
 Identities = 14/27 (51%), Positives = 20/27 (74%)
 Frame = +3

Query: 600 PSSPSIASRRSLTAITWSSASTNAPRS 680
           PSS S++   S T+ T+SSAST++P S
Sbjct: 587 PSSTSLSISSSSTSSTFSSASTSSPSS 613


>SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 598

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 24/79 (30%), Positives = 40/79 (50%), Gaps = 1/79 (1%)
 Frame = +3

Query: 486 EAENCRNASAGSVQFSAASTA*IGPSIKSKRKP-LMKEKPSSPSIASRRSLTAITWSSAS 662
           EA+  RN S+ S+   AAS   +  S++S R+P + +E+P      ++R  +AIT   A 
Sbjct: 194 EAQLPRNKSSVSIAEQAASRPKVSFSLQSSRQPSIAEEQPQ-----TQRKSSAITVEHAE 248

Query: 663 TNAPRS*CLRNRLKTATSP 719
              P +   RN +  +  P
Sbjct: 249 NAEPET--PRNNVSFSRKP 265


>SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 561

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +3

Query: 561 SIKSKRKPLMKEKPSSPSIASRRSLTAITWSSASTNAPRS 680
           S+    KP M   PS+PS++  R++TA      S    RS
Sbjct: 369 SVPENGKPNMGRIPSAPSLSKGRAMTADNMDMLSLTTRRS 408


>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 4196

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 14/46 (30%), Positives = 20/46 (43%)
 Frame = -3

Query: 661  DADDQVIAVKLLRLAIDGDDGFSFINGFRFDFIDGPIQAVDAAENC 524
            D DD+ + +K+  L         FI  F +  +D PI   D   NC
Sbjct: 1835 DHDDEAVVIKMFSL--------EFIYAFEYSELDDPIVYTDLTRNC 1872


>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
           subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1729

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 13/50 (26%), Positives = 24/50 (48%)
 Frame = +1

Query: 379 WREQQLALPRRCQGMQEVPNEPRKIWQHSILMAHVWKRRIAAMLLQALCS 528
           WRE    +PRR        ++   +++  +L++ VW   I +M  + L S
Sbjct: 603 WRELFYRVPRRIYTKLLYTDDMEIVFKPKVLISQVWNAIIISMYREHLIS 652


>SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 905

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 17/62 (27%), Positives = 24/62 (38%), Gaps = 2/62 (3%)
 Frame = +2

Query: 308 TLKEYLTHKGLTPNLIHYILYAIAGGN--NSLPCLEGVKECKKFLMSLGRYGNTPFLWPM 481
           TLK  L   G+TP    + +  + G    +S    EG     KF + L        L P+
Sbjct: 124 TLKSLLAEAGVTPKAFEFAVNNVRGNKRIDSKNAEEGFDALNKFTVDLTELARNGQLDPV 183

Query: 482 YG 487
            G
Sbjct: 184 IG 185


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,756,683
Number of Sequences: 5004
Number of extensions: 56083
Number of successful extensions: 213
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)