Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0229
(720 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59920.1 68416.m06687 Rab GDP dissociation inhibitor (GDI2) i... 130 1e-30
At2g44100.1 68415.m05484 Rab GDP dissociation inhibitor (GDI1) i... 122 2e-28
At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putativ... 112 3e-25
At3g06540.1 68416.m00758 GDP dissociation inhibitor family prote... 88 6e-18
At3g07250.1 68416.m00863 nuclear transport factor 2 (NTF2) famil... 30 1.8
At4g02350.1 68417.m00319 exocyst complex subunit Sec15-like fami... 29 2.3
At1g12850.1 68414.m01493 phosphoglycerate/bisphosphoglycerate mu... 29 4.1
At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative severa... 28 5.4
At2g20100.1 68415.m02348 ethylene-responsive family protein simi... 28 5.4
At1g56710.1 68414.m06522 glycoside hydrolase family 28 protein /... 28 5.4
At4g27020.1 68417.m03886 expressed protein gene F20P5.12 of BAC ... 28 7.2
At3g02360.2 68416.m00220 6-phosphogluconate dehydrogenase family... 28 7.2
At3g02360.1 68416.m00219 6-phosphogluconate dehydrogenase family... 28 7.2
At4g00800.1 68417.m00110 expressed protein contains 1 WD-40 repe... 27 9.5
At2g15000.2 68415.m01708 expressed protein 27 9.5
At2g15000.1 68415.m01707 expressed protein 27 9.5
At1g62570.1 68414.m07059 flavin-containing monooxygenase family ... 27 9.5
>At3g59920.1 68416.m06687 Rab GDP dissociation inhibitor (GDI2)
identical to Rab GDP dissociation inhibitor AtGDI2
[Arabidopsis thaliana] GI:2446981
Length = 444
Score = 130 bits (313), Expect = 1e-30
Identities = 73/227 (32%), Positives = 116/227 (51%), Gaps = 6/227 (2%)
Frame = +2
Query: 20 RKFNIDTTPKLLFSRGPLVELLISSNIARYAEFRCVTRVLTWLNDQLMPVPCSRADVFAT 199
R +N+D PK + G LV LI +++ +Y F+ V ++ ++ VP + + +
Sbjct: 69 RDYNVDMMPKFMMGNGKLVRTLIHTDVTKYLSFKAVDGSYVFVKGKVQKVPATPMEALKS 128
Query: 200 EAVGIVEKRMLMKMLTSIVGYSEEE-MNNEFKDWNNKTLKEYLTHKGLTPNLIHYILYAI 376
+GI EKR K + + Y E++ ++ D T KE + GL N I +I +A+
Sbjct: 129 SLMGIFEKRRAGKFFSFVQEYDEKDPKTHDGMDLTRVTTKELIAKYGLDGNTIDFIGHAV 188
Query: 377 AGGNNSL----PCLEGVKECKKFLMSLGRY-GNTPFLWPMYGSGELPQCFCRLCAVFGGV 541
A N P + V K + SL R+ G +P+++P+YG GELPQ F RL AV+GG
Sbjct: 189 ALHTNDQHLDQPAFDTVMRMKLYAESLARFQGTSPYIYPLYGLGELPQAFARLSAVYGGT 248
Query: 542 YCLNRPIDKVETKTVDEGKTVVTIDSKSKKLNCDHLVIGVHECPEEL 682
Y LN+P KVE DEG V+ + S+ + C +V P ++
Sbjct: 249 YMLNKPECKVE---FDEGGKVIGVTSEGETAKCKKIVCDPSYLPNKV 292
>At2g44100.1 68415.m05484 Rab GDP dissociation inhibitor (GDI1)
identical to GDP dissociation inhibitor [Arabidopsis
thaliana] GI:1655424
Length = 445
Score = 122 bits (294), Expect = 2e-28
Identities = 74/218 (33%), Positives = 120/218 (55%), Gaps = 10/218 (4%)
Frame = +2
Query: 20 RKFNIDTTPKLLFSRGPLVELLISSNIARYAEFRCVTRVLTWLNDQLMPVPCSRADVFAT 199
R +N+D PK + + G LV +LI +++ +Y F+ V ++ ++ VP + + +
Sbjct: 69 RDYNVDMMPKFMMANGKLVRVLIHTDVTKYLSFKAVDGSYVFVQGKVQKVPATPMEALKS 128
Query: 200 EAVGIVEKRMLMKMLTSIVGYSEEE-MNNEFKDWNNKTLKEYLTHKGLTPNLIHYILYAI 376
+GI EKR K + + Y E++ ++ D T K+ + GL + I +I +A+
Sbjct: 129 PLMGIFEKRRAGKFFSYVQEYDEKDPKTHDGMDLRRVTTKDLIAKFGLKEDTIDFIGHAV 188
Query: 377 A--GGNNSL--PCLEGVKECKKFLMSLGRY-GNTPFLWPMYGSGELPQCFCRLCAVFGGV 541
A +N L P + V K + SL R+ G +P+++P+YG GELPQ F RL AV+GG
Sbjct: 189 ALHCNDNHLHQPAYDTVMRMKLYAESLARFQGGSPYIYPLYGLGELPQAFARLSAVYGGT 248
Query: 542 YCLNRPIDKVETKTVDEGK-TVVTID---SKSKKLNCD 643
Y LN+P KVE +EGK + VT + +K KK+ CD
Sbjct: 249 YMLNKPECKVEFD--EEGKVSGVTSEGETAKCKKVVCD 284
>At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putative
strong similarity to GDP dissociation inhibitor protein
OsGDI1 [Oryza sativa] GI:2384758; contains Pfam profile
PF00996: GDP dissociation inhibitor
Length = 365
Score = 112 bits (269), Expect = 3e-25
Identities = 66/203 (32%), Positives = 109/203 (53%), Gaps = 8/203 (3%)
Frame = +2
Query: 59 SRGPLVELLISSNIARYAEFRCVTRVLTWLNDQLMPVPCSRADVFATEAVGIVEKRMLMK 238
+ G LV+ LI +++ +Y F+ V + ++ VP + + + +G+ EKR K
Sbjct: 2 ANGLLVQTLIHTDVTKYLNFKAVDGSFVYKKGKIYKVPATDVEALKSPLMGLFEKRRARK 61
Query: 239 MLTSIVGYSEEE-MNNEFKDWNNKTLKEYLTHKGLTPNLIHYILYAIAGGNNS----LPC 403
+ Y E++ ++E D + T +E ++ GL + I +I +A+A N+ P
Sbjct: 62 FFIYVQDYDEKDPKSHEGLDLSKVTAREIISKYGLEDDTIDFIGHALALHNDDDYLDQPA 121
Query: 404 LEGVKECKKFLMSLGRY-GNTPFLWPMYGSGELPQCFCRLCAVFGGVYCLNRPIDKVETK 580
++ VK K + SL R+ G +P+++P+YG GELPQ F RL AV+GG Y LN+P KVE
Sbjct: 122 IDFVKRIKLYAESLARFQGGSPYIYPLYGLGELPQAFARLSAVYGGTYMLNKPECKVEFD 181
Query: 581 TVDE--GKTVVTIDSKSKKLNCD 643
+ G T +K KK+ CD
Sbjct: 182 GSGKAIGVTSAGETAKCKKVVCD 204
>At3g06540.1 68416.m00758 GDP dissociation inhibitor family protein
/ Rab GTPase activator family protein similar to
SP|P26374 Rab proteins geranylgeranyltransferase
component A 2 (Rab escort protein 2) {Homo sapiens};
contains Pfam profile PF00996: GDP dissociation
inhibitor
Length = 563
Score = 87.8 bits (208), Expect = 6e-18
Identities = 61/202 (30%), Positives = 104/202 (51%), Gaps = 21/202 (10%)
Frame = +2
Query: 20 RKFNIDTT-PKLLFSRGPLVELLISSNIARYAEFRCVTRVLTW-LNDQLMPVPCSRADVF 193
R+FN+D P+++F + L++ S Y EF+ + + +L VP SRA +F
Sbjct: 118 RRFNVDLAGPRVVFCADESINLMLKSGANNYVEFKSIDASFVGDSSGELRNVPDSRAAIF 177
Query: 194 ATEAVGIVEKRMLMK---MLTSIVGYSEEEMNNEFKDWNNKTLK----EYLTHKGLTPNL 352
+++ ++EK LMK ++ S + S E+ ++ + + ++ ++L+ L P +
Sbjct: 178 KDKSLTLLEKNQLMKFFKLVQSHLASSTEKDDSTTVKISEEDMESPFVDFLSKMRLPPKI 237
Query: 353 IHYILYAIA----GGNNSLPCL------EGVKECKKFLMSLGRYGNT--PFLWPMYGSGE 496
ILYAIA +N+ C EG+ ++ S+GR+ N ++P+YG GE
Sbjct: 238 KSIILYAIAMLDYDQDNTETCRHLLKTKEGIDRLALYITSMGRFSNALGALIYPIYGQGE 297
Query: 497 LPQCFCRLCAVFGGVYCLNRPI 562
LPQ FCR AV G +Y L PI
Sbjct: 298 LPQAFCRRAAVKGCIYVLRMPI 319
>At3g07250.1 68416.m00863 nuclear transport factor 2 (NTF2) family
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF00076: RNA recognition motif.
(a.k.a. RRM, RBD, or RNP domain), PF02136: Nuclear
transport factor 2 (NTF2) domain
Length = 1294
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = -3
Query: 433 ELLAFLDTFEAGQAVVPASDRVQDVVNEVRGQAL 332
++ F+D EA A+ PA+D +++ V E G AL
Sbjct: 390 DMFRFVDIPEANAAIPPANDVIEEKVPETEGAAL 423
>At4g02350.1 68417.m00319 exocyst complex subunit Sec15-like family
protein contains Pfam profile PF04091: Exocyst complex
subunit Sec15-like
Length = 771
Score = 29.5 bits (63), Expect = 2.3
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 221 KRMLMKMLTSIVGYSEEEMNNEFKDW 298
KRML + +I Y E ++N EF DW
Sbjct: 187 KRMLENRIPAIRSYVERKVNKEFGDW 212
>At1g12850.1 68414.m01493 phosphoglycerate/bisphosphoglycerate
mutase family protein similar to XY4 protein [Silene
vulgaris] GI:21386788; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 405
Score = 28.7 bits (61), Expect = 4.1
Identities = 24/56 (42%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
Frame = +3
Query: 504 NASAGSVQFSAASTA*IGPSIK--SKRKPLMKEKPSSPSIASRRSLTAITWSSAST 665
+AS S Q S+ T IGPSIK L PSSP + SR S A SA T
Sbjct: 66 SASPLSPQLSSLGTPRIGPSIKVWDPYNVLAPPPPSSPPLFSRISSAAEHDRSAVT 121
>At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative several
putative ATP-dependent helicases
Length = 1058
Score = 28.3 bits (60), Expect = 5.4
Identities = 19/51 (37%), Positives = 25/51 (49%)
Frame = -3
Query: 277 HLLFAITDNRCQHFHEHPFLDDTDRFGGEYVGSGARNRHQLIVEPGQDAGH 125
HL+ AIT R + E ++D FGG+ G A N +E G D GH
Sbjct: 601 HLVEAITSYRGGYIAEDRRKIESDLFGGKLCGIAATN----ALELGIDVGH 647
>At2g20100.1 68415.m02348 ethylene-responsive family protein similar
to Ethylene-regulated ER33 protein (GI:5669656)
[Lycopersicon esculentum]; PMID: 12679534; putative
bHLH133 transcription factor
Length = 362
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
Frame = -1
Query: 492 PLPYMGHKN-GVLPYLPRLIRNFLHSLTPSRQGKLLFPP 379
P P M H+ ++P L+ NF S T S FPP
Sbjct: 33 PTPLMSHEPPSTTAFIPSLLPNFFSSPTSSSSSSPSFPP 71
>At1g56710.1 68414.m06522 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase PG1 GI:5669846, PG2 GI:5669848 from
(Glycine max); contains PF00295: Glycosyl hydrolases
family 28 (polygalacturonases)
Length = 434
Score = 28.3 bits (60), Expect = 5.4
Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 2/51 (3%)
Frame = +2
Query: 380 GGNNSLPCLEGVKECKKFLMSLGRYGNTPFLWPMYGSGEL--PQCFCRLCA 526
G ++S+PC S G++ PF W YGS ++ P +C L A
Sbjct: 380 GCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMKITVPPVYCLLDA 430
>At4g27020.1 68417.m03886 expressed protein gene F20P5.12 of BAC
F20P5 from Arabidopsis thalianachromosome 1,
PID:g2194125
Length = 523
Score = 27.9 bits (59), Expect = 7.2
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 260 YSEEEMNNEFKDWNNKTLKEYLTHKGLT 343
++ E EFK+W K EY+ KG++
Sbjct: 130 FASREHTMEFKEWEGKAEYEYVKQKGVS 157
>At3g02360.2 68416.m00220 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
;similar to 6-phosphogluconate dehydrogenase GB:BAA22812
GI:2529229 [Glycine max]
Length = 486
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +2
Query: 239 MLTSIVGYSEEEMNNEFKDWNNKTLKEYL 325
+L S+ S EE+++ F DWN L+ +L
Sbjct: 208 VLKSVGKLSNEELHSVFSDWNKGELESFL 236
>At3g02360.1 68416.m00219 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
;similar to 6-phosphogluconate dehydrogenase GB:BAA22812
GI:2529229 [Glycine max]
Length = 486
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +2
Query: 239 MLTSIVGYSEEEMNNEFKDWNNKTLKEYL 325
+L S+ S EE+++ F DWN L+ +L
Sbjct: 208 VLKSVGKLSNEELHSVFSDWNKGELESFL 236
>At4g00800.1 68417.m00110 expressed protein contains 1 WD-40 repeat
(PF00400); 42% similarity to fimbriae-associated protein
Fap1 (gi:3929312) [Streptococcus parasanguinis]
Length = 994
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/58 (25%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +2
Query: 50 LLFSRGPLVELLISSNIARYAEFRCVTRVL--TWLNDQLMPVPCSRADVFATEAVGIV 217
+ + R V L+ S+I YA++ + + WL+DQL+ +P ++ G+V
Sbjct: 703 IAWDRRVQVAKLVKSDIKEYAKWSLDSPAIGVVWLDDQLLVIPTVTGHLYLFTRDGVV 760
>At2g15000.2 68415.m01708 expressed protein
Length = 97
Score = 27.5 bits (58), Expect = 9.5
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = +3
Query: 510 SAGSVQFSAASTA*IGPSIKSKRKPLMKEKPSSPSIASRRSLTAITWSSASTNAPRS*CL 689
+AGS S S PS++S L + +P S+ SRR T+SS S N C
Sbjct: 5 NAGSAARSFVSATARSPSLRSPTTALPRLRPPQSSLPSRR----FTFSSPSRNLGALGCT 60
Query: 690 RNRL 701
++ L
Sbjct: 61 QSFL 64
>At2g15000.1 68415.m01707 expressed protein
Length = 93
Score = 27.5 bits (58), Expect = 9.5
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = +3
Query: 510 SAGSVQFSAASTA*IGPSIKSKRKPLMKEKPSSPSIASRRSLTAITWSSASTNAPRS*CL 689
+AGS S S PS++S L + +P S+ SRR T+SS S N C
Sbjct: 5 NAGSAARSFVSATARSPSLRSPTTALPRLRPPQSSLPSRR----FTFSSPSRNLGALGCT 60
Query: 690 RNRL 701
++ L
Sbjct: 61 QSFL 64
>At1g62570.1 68414.m07059 flavin-containing monooxygenase family
protein / FMO family protein low similarity to
flavin-containing monooxygenase FMO3 [Rattus norvegicus]
GI:12006730; contains Pfam profile PF00743:
Flavin-binding monooxygenase-like
Length = 461
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +1
Query: 325 DSQGLDPEPHSLHPVRDRWREQQLALPRRCQGMQEVPNEPR 447
D GLDP +H ++ + LPR C G ++ P PR
Sbjct: 58 DELGLDPTRPIVHS--SVYKSLRTNLPRECMGYKDFPFVPR 96
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,886,826
Number of Sequences: 28952
Number of extensions: 317620
Number of successful extensions: 1199
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1195
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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