Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0227
         (761 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    34   0.019
SPAC23C11.14 |zhf1|zhf, zhf|zinc ion transporter Zhf1|Schizosacc...    30   0.41 
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom...    30   0.41 
SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces po...    30   0.41 
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha...    28   1.7  
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch...    27   2.2  
SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces pomb...    27   2.2  
SPAC323.08 |||ribonuclease MRP complex subunit |Schizosaccharomy...    27   2.9  
SPAC630.12 |||phosphoprotein phosphatase |Schizosaccharomyces po...    27   2.9  
SPAC22E12.14c |sck2||serine/threonine protein kinase Sck2|Schizo...    27   3.9  
SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom...    26   5.1  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   5.1  
SPBC4.01 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Ma...    26   5.1  
SPCC4B3.17 |cbp3||ubiquinol cytochrome-c reductase assembly prot...    26   5.1  
SPAC144.16 |||DUF59 family protein|Schizosaccharomyces pombe|chr...    26   6.7  
SPBC30B4.08 |eri1||double-strand siRNA ribonuclease|Schizosaccha...    26   6.7  
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    25   8.9  
SPBC1734.10c |||mRNA processing protein |Schizosaccharomyces pom...    25   8.9  
SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch...    25   8.9  

>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 34.3 bits (75), Expect = 0.019
 Identities = 27/76 (35%), Positives = 41/76 (53%), Gaps = 3/76 (3%)
 Frame = +2

Query: 197 ISSRILLKSNPLSSPKDKKTASVPNSFTKMSRSNSLLYGAASALRF---LSNESISVLTD 367
           ISS I   S  LSSP    ++ + +S + +S S+S+L  + S +     LS  S SV+  
Sbjct: 614 ISSSISSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPS 673

Query: 368 LRPLVSPSSTFFLASS 415
              LVS SS+  ++SS
Sbjct: 674 SSTLVSSSSSLIVSSS 689


>SPAC23C11.14 |zhf1|zhf, zhf|zinc ion transporter
           Zhf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 387

 Score = 29.9 bits (64), Expect = 0.41
 Identities = 20/55 (36%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = -3

Query: 279 VKLFGTDAVFLSFGDDKGFDFNKILDDILGTCSS-GISKLKHFQTDFRTHLLFLD 118
           VKL  T  V ++  DDKG  + K+  DI     S GI  +   Q +F  H L  D
Sbjct: 329 VKLIATVHVCVTLPDDKGESYTKLTTDIRNVLQSFGIYDVT-IQPEFANHPLLCD 382


>SPBC354.13 |rga6||GTPase activating protein
           Rga6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 733

 Score = 29.9 bits (64), Expect = 0.41
 Identities = 14/39 (35%), Positives = 20/39 (51%)
 Frame = -3

Query: 423 KRFEEAKKKVEDGLTRGRRSVKTEIDSFDKNLKAEAAPY 307
           KRFEE   +V   L +    + T I S   NL+ + AP+
Sbjct: 33  KRFEEPSLEVGGSLAKVENGLSTSISSSASNLEGQQAPF 71


>SPCC16C4.18c |taf50||histone H4-like TAF |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 452

 Score = 29.9 bits (64), Expect = 0.41
 Identities = 28/107 (26%), Positives = 43/107 (40%), Gaps = 3/107 (2%)
 Frame = -3

Query: 465 LRTESPQAIYDSLVKRFEEAKKKVEDGLTRGRRSVKT--EIDSFDKNLKAEAA-PYNNEL 295
           L  E   AI   L  R  +  ++    +   +R+V T  +I S  + L  E    +NN  
Sbjct: 23  LADEPAAAIAMDLEYRIHQVVQEATKFMVHSKRTVLTSADISSALRTLNVEPLYGFNNSR 82

Query: 294 DLDIFVKLFGTDAVFLSFGDDKGFDFNKILDDILGTCSSGISKLKHF 154
            L+      G     L + DD+  DF KI++  L      IS   H+
Sbjct: 83  PLEFHEAAVGAGQNSLYYLDDEEVDFEKIINAPLPKVPRNISYSAHW 129


>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 4196

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 13/49 (26%), Positives = 23/49 (46%)
 Frame = -3

Query: 261  DAVFLSFGDDKGFDFNKILDDILGTCSSGISKLKHFQTDFRTHLLFLDA 115
            + +FLSFG  +    +++LD     CS  +S   H+    R   + + A
Sbjct: 2021 EILFLSFGFKESSLLSQVLDSFFLCCSGSLSNCLHYDFGLRAMKVVIKA 2069


>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
           Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 19/67 (28%), Positives = 37/67 (55%)
 Frame = +2

Query: 113 SASRNKR*VRKSVWKCFSLLIPLLHVPRISSRILLKSNPLSSPKDKKTASVPNSFTKMSR 292
           S SR+ R   KS  + +S    +  +  I+ +  ++ +   SP+ ++ +S  NS++ +SR
Sbjct: 643 SYSRSTRSYSKS--RSYSRSRSVTPINNINHKKYIRKDRELSPRGRERSSNRNSYSDLSR 700

Query: 293 SNSLLYG 313
           S+SL  G
Sbjct: 701 SSSLSRG 707


>SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 515

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 16/49 (32%), Positives = 25/49 (51%)
 Frame = +2

Query: 278 TKMSRSNSLLYGAASALRFLSNESISVLTDLRPLVSPSSTFFLASSNLL 424
           +KM  S   L   A +L   SN+  ++   ++P    SS+F+L S  LL
Sbjct: 317 SKMLSSTENLLLIAESLSRASNDFAAISKQMQPFSIESSSFYLPSHRLL 365


>SPAC323.08 |||ribonuclease MRP complex subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 211

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 13/25 (52%), Positives = 15/25 (60%)
 Frame = +2

Query: 209 ILLKSNPLSSPKDKKTASVPNSFTK 283
           I L  NP S+  +K T S PNSF K
Sbjct: 138 ISLSINPASTSYEKLTVSSPNSFLK 162


>SPAC630.12 |||phosphoprotein phosphatase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 422

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 7/29 (24%), Positives = 21/29 (72%)
 Frame = -1

Query: 560 RYSVTTSMSWSLEVAKVTWTAFLNISWVP 474
           R+S++T++    ++ ++T + F++ +W+P
Sbjct: 382 RFSLSTTIHLFKKIVRITLSTFISYTWIP 410


>SPAC22E12.14c |sck2||serine/threonine protein kinase
           Sck2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 646

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 12/32 (37%), Positives = 22/32 (68%)
 Frame = +2

Query: 185 HVPRISSRILLKSNPLSSPKDKKTASVPNSFT 280
           +V R SS  ++ S+P+ +P  ++T+S+PN  T
Sbjct: 155 NVSRSSS--MMSSHPIPTPAIQRTSSIPNPLT 184


>SPAC6F6.12 |||autophagy associated protein
           Atg24|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 401

 Score = 26.2 bits (55), Expect = 5.1
 Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
 Frame = -3

Query: 249 LSFGDDKGFDFNKILDDILGT--CSSGISKLKHFQTDFR 139
           +S G   GF  +K LDD+ G     + + +L+H Q++ +
Sbjct: 301 ISSGGSNGFSLSKTLDDLRGIDHNDTRLKRLEHVQSELQ 339


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.2 bits (55), Expect = 5.1
 Identities = 28/82 (34%), Positives = 44/82 (53%), Gaps = 5/82 (6%)
 Frame = +2

Query: 197 ISSRILLKSNPL-SSPKDKKTASVPNSFTKMSRSNSLLYGAA----SALRFLSNESISVL 361
           ISS  L  S+PL SS     ++S  +S   +S ++S  + +A    ++  +LS+ S+ V 
Sbjct: 159 ISSSSLSSSDPLTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSV-VS 217

Query: 362 TDLRPLVSPSSTFFLASSNLLT 427
           +   P  S SST  L SS+L T
Sbjct: 218 SSSSPSSSSSST--LTSSSLST 237


>SPBC4.01 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 248

 Score = 26.2 bits (55), Expect = 5.1
 Identities = 13/36 (36%), Positives = 20/36 (55%)
 Frame = -3

Query: 132 LLFLDAELAYPTSTGLPLHLNLVGSATAKLDLATNI 25
           LL L   LA+ ++    + +NL G ATAK  +A  +
Sbjct: 156 LLALQMALAHISANSYAVGMNLTGKATAKFGVAAAV 191


>SPCC4B3.17 |cbp3||ubiquinol cytochrome-c reductase assembly protein
           Cbp3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 283

 Score = 26.2 bits (55), Expect = 5.1
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +2

Query: 215 LKSNPLSSPKDKKTASVPNSFTK 283
           L+  PL+ P+D +  + PNSF K
Sbjct: 56  LRYKPLTPPQDPRKVAPPNSFLK 78


>SPAC144.16 |||DUF59 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 179

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
 Frame = -3

Query: 465 LRTESPQAI-YDSLVKRFEEAKKKVEDGLTRGRRSVKTEIDSFDKNLKAEAAPYNNELDL 289
           L+ E+P+    + L  R EE     ED L    +   TEI+S   N+K E  P + +   
Sbjct: 5   LQNENPEVKELNQLPSRVEEE----EDLLLSSTKQWLTEIESEQTNIKEERDPIDPQEIY 60

Query: 288 DIFVKL 271
           D+  K+
Sbjct: 61  DLLAKI 66


>SPBC30B4.08 |eri1||double-strand siRNA
           ribonuclease|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 313

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 17/57 (29%), Positives = 28/57 (49%)
 Frame = -1

Query: 461 VRKALKLFTIVWSRGSKKLRRRWKMD*REDVDLSRRKLIRLTKISKRKQLHTTMSWI 291
           +R AL+   +  +   +KL+RRWK   RE     +RK  R  K S   +  T + ++
Sbjct: 28  IRIALQELGLSTNGNKEKLKRRWKF--REKRLEEKRKQERYQKFSTSNENKTCLRYL 82


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 20/69 (28%), Positives = 30/69 (43%)
 Frame = +2

Query: 311  GAASALRFLSNESISVLTDLRPLVSPSSTFFLASSNLLTRLS*IA*GLSVRRKLLGPKKC 490
            G A    F   ++ S+ ++  P    S+TF L   +LLTR   +  G  +   L    K 
Sbjct: 997  GQAREAEFFYRQACSI-SEKMPFSCFSATFQLRLCSLLTRAGKLEKGEKILFDLTEAMKS 1055

Query: 491  SRTRSKLPW 517
            + T  KL W
Sbjct: 1056 TDTYHKLLW 1064


>SPBC1734.10c |||mRNA processing protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 332

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
 Frame = +2

Query: 143 KSVWK-CFSLLIPLLHVPRISSRILLKSNPLSSPKDKKTASVPNSFTKMS 289
           +S+W  CF   +P+       S I   ++P+S+ K      VP  FTK S
Sbjct: 233 RSIWTWCFVPHLPISFQKSPFSSIEFANSPISTIKLLYQLEVPPDFTKSS 282


>SPBC3F6.01c |||serine/threonine protein phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 473

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 25/85 (29%), Positives = 36/85 (42%)
 Frame = -3

Query: 276 KLFGTDAVFLSFGDDKGFDFNKILDDILGTCSSGISKLKHFQTDFRTHLLFLDAELAYPT 97
           KL   DAVF++ G+ +  D NK+     G C S  ++ + F     T  L     L   +
Sbjct: 265 KLLYPDAVFINRGNHETDDMNKVY-GFEGECRSKYNE-RTFNIFSETFSLLPLGSLI--S 320

Query: 96  STGLPLHLNLVGSATAKLDLATNID 22
            + L +H  L       LD   NID
Sbjct: 321 DSYLVVHGGLFSDDNVTLDQLRNID 345


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,140,869
Number of Sequences: 5004
Number of extensions: 64935
Number of successful extensions: 230
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)