Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0227
         (761 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g45400.1 68415.m05646 dihydroflavonol 4-reductase family / di...    34   0.12 
At3g33520.1 68416.m04291 actin-related protein 6 (ARP6) nearly i...    31   1.1  
At1g13160.1 68414.m01526 SDA1 family protein contains Pfam PF052...    30   1.5  
At5g25580.1 68418.m03044 expressed protein                             29   3.4  
At5g67470.1 68418.m08507 formin homology 2 domain-containing pro...    29   4.5  
At1g31740.1 68414.m03894 beta-galactosidase, putative / lactase,...    29   4.5  
At1g16010.1 68414.m01920 magnesium transporter CorA-like family ...    29   4.5  

>At2g45400.1 68415.m05646 dihydroflavonol 4-reductase family /
           dihydrokaempferol 4-reductase family similar to
           dihydroflavonol 4-reductase (SP:P51102), vestitone
           reductase (Medicago sativa, GI:973249)
          Length = 364

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 26/88 (29%), Positives = 42/88 (47%), Gaps = 5/88 (5%)
 Frame = -3

Query: 645 GAGGSVDEPVIYSQDSYL---PRSVNFNLTVSLFGHDFNVLEFGGRQG--NLDRVLEHFL 481
           G GG VDE V    + +     + V+ +  VS    +   LEFGG+ G   +  V+   +
Sbjct: 174 GGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFGGKNGLEVVTLVIPLVV 233

Query: 480 GPKSFLRTESPQAIYDSLVKRFEEAKKK 397
           GP  F+ +  P +++ SL   F   K+K
Sbjct: 234 GP--FISSSLPSSVFISLAMLFGNYKEK 259


>At3g33520.1 68416.m04291 actin-related protein 6 (ARP6) nearly
           identical to actin-related protein 6 (ARP6) [Arabidopsis
           thaliana] GI:21427467; contains Pfam profile PF00022:
           Actin
          Length = 421

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 18/57 (31%), Positives = 30/57 (52%)
 Frame = +2

Query: 86  RPVEVGYASSASRNKR*VRKSVWKCFSLLIPLLHVPRISSRILLKSNPLSSPKDKKT 256
           RP++ GY  ++      +++ +W    L   LLH+   SS +LL   PLS P  ++T
Sbjct: 63  RPIDRGYLINSD-----LQREIWS--HLFTSLLHIAPSSSSLLLTEAPLSIPSVQRT 112


>At1g13160.1 68414.m01526 SDA1 family protein contains Pfam PF05285:
           SDA1 domain; similar to mystery 45A
           (GI:16797816){Drosophila melanogaster}
          Length = 804

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
 Frame = -3

Query: 447 QAIYDSLVKRFEEAKKKVEDGLTRGRRSVKTEIDSFDKNLKAEAAPYNNELDLDIFV-KL 271
           QA+Y +  K    +KKK +  L R  +S+K +  S  +N  +  +P N+  D   F  KL
Sbjct: 278 QAVYKANNKGTSSSKKKKQAKLQRAVKSIKRKQRSSSENTTSTFSPLNHLNDAQKFAEKL 337

Query: 270 F 268
           F
Sbjct: 338 F 338


>At5g25580.1 68418.m03044 expressed protein 
          Length = 405

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 14/49 (28%), Positives = 23/49 (46%)
 Frame = -3

Query: 438 YDSLVKRFEEAKKKVEDGLTRGRRSVKTEIDSFDKNLKAEAAPYNNELD 292
           YD ++    E  +  +     GR      +DS DK+ + EAA  +N+ D
Sbjct: 324 YDKMISDAVEETEDTDGDEVEGRDQQSASLDSNDKSTEVEAAEIHNDDD 372


>At5g67470.1 68418.m08507 formin homology 2 domain-containing
           protein / FH2 domain-containing protein contains formin
           homology 2 domain, Pfam:PF02181
          Length = 899

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 4/58 (6%)
 Frame = -3

Query: 474 KSFLRTESPQA-IYDSL---VKRFEEAKKKVEDGLTRGRRSVKTEIDSFDKNLKAEAA 313
           +SFL+TE+ Q   +DS+   +K  EE  +K++ G  +    VK   + F  N   E A
Sbjct: 768 RSFLKTETTQGRFFDSMKTFLKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEA 825


>At1g31740.1 68414.m03894 beta-galactosidase, putative / lactase,
           putative similar to beta-galactosidase precursor
           [Brassica oleracea] SWISS-PROT:P49676
          Length = 786

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 11/28 (39%), Positives = 19/28 (67%)
 Frame = +2

Query: 17  RTSMLVARSNLAVAEPTKLRWRGRPVEV 100
           +TS++V ++N A  EP+ L+W  RP  +
Sbjct: 437 QTSVMVKKANEAENEPSTLKWSWRPENI 464


>At1g16010.1 68414.m01920 magnesium transporter CorA-like family
           protein (MRS2-1) low similarity to SP|Q01926 RNA
           splicing protein MRS2, mitochondrial precursor
           {Saccharomyces cerevisiae}; contains Pfam profile
           PF01544: CorA-like Mg2+ transporter protein
          Length = 442

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 16/50 (32%), Positives = 24/50 (48%)
 Frame = +2

Query: 281 KMSRSNSLLYGAASALRFLSNESISVLTDLRPLVSPSSTFFLASSNLLTR 430
           K  R    +YG  S L + SN+ +SV   + P+ SP  +  L  S  + R
Sbjct: 263 KKRRMEGSMYGDQSLLGYRSNDGLSVSAPVSPVSSPPDSRRLDKSLSIAR 312


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,340,381
Number of Sequences: 28952
Number of extensions: 338233
Number of successful extensions: 1075
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1042
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1075
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)