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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0224
         (768 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g63530.1 68414.m07182 hypothetical protein                          29   4.5  
At5g49665.1 68418.m06148 zinc finger (C3HC4-type RING finger) fa...    28   7.9  
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei...    28   7.9  
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei...    28   7.9  

>At1g63530.1 68414.m07182 hypothetical protein
          Length = 499

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +2

Query: 131 EGQNIKITCKLIGKPYSEVTWKYKKDELDNGT 226
           EGQNI      + K + E+ W+Y K E   G+
Sbjct: 279 EGQNISSFKPYVDKSHDELRWEYYKKEAKGGS 310


>At5g49665.1 68418.m06148 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profiles PF00097: Zinc
           finger, C3HC4 type (RING finger), PF00092: von
           Willebrand factor type A domain
          Length = 740

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 14/48 (29%), Positives = 21/48 (43%)
 Frame = +3

Query: 270 TSRAWTTPCWCCRTRSAPTPACTSAARPGARPPTSRSGSRACTTRSGP 413
           T R+ ++P   CRT  A TP     + P +   + R    A +  S P
Sbjct: 56  TPRSTSSPSLRCRTADAQTPTAEQTSTPRSATKSPRLSLAAISNPSSP 103


>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 294

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 21/100 (21%), Positives = 42/100 (42%)
 Frame = +2

Query: 98  HAKMTKDANVVEGQNIKITCKLIGKPYSEVTWKYKKDELDNGTDVSAVLGSRVQLEKNEQ 277
           HAK   +    +G  + +  ++   PYS  ++    +E+D G D S    S   L +  +
Sbjct: 107 HAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASP---STAMLSEVSK 163

Query: 278 GLDNTVLVLQNAERADAGLYQCSPAGGTPADVTLRVKGVY 397
            L  T++     ER    LY      G+  ++  + + ++
Sbjct: 164 RLKITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 203


>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 369

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 21/100 (21%), Positives = 42/100 (42%)
 Frame = +2

Query: 98  HAKMTKDANVVEGQNIKITCKLIGKPYSEVTWKYKKDELDNGTDVSAVLGSRVQLEKNEQ 277
           HAK   +    +G  + +  ++   PYS  ++    +E+D G D S    S   L +  +
Sbjct: 107 HAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASP---STAMLSEVSK 163

Query: 278 GLDNTVLVLQNAERADAGLYQCSPAGGTPADVTLRVKGVY 397
            L  T++     ER    LY      G+  ++  + + ++
Sbjct: 164 RLKITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 203


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,121,609
Number of Sequences: 28952
Number of extensions: 209896
Number of successful extensions: 727
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 726
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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