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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0221
         (699 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_58392| Best HMM Match : Peptidase_M16_C (HMM E-Value=1.2e-24)       91   8e-19
SB_41612| Best HMM Match : OstA (HMM E-Value=5.2)                      30   2.1  
SB_27302| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.7  
SB_57619| Best HMM Match : DUF229 (HMM E-Value=0)                      29   4.8  
SB_12535| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.8  
SB_24400| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.8  
SB_32009| Best HMM Match : PKD (HMM E-Value=1.9e-19)                   28   6.3  
SB_33997| Best HMM Match : Astacin (HMM E-Value=0)                     28   8.4  
SB_32164| Best HMM Match : ECH (HMM E-Value=0.02)                      28   8.4  

>SB_58392| Best HMM Match : Peptidase_M16_C (HMM E-Value=1.2e-24)
          Length = 1064

 Score = 91.1 bits (216), Expect = 8e-19
 Identities = 48/138 (34%), Positives = 71/138 (51%)
 Frame = +3

Query: 6   THITEHVGQSGQYEQGILINSHCLDHNLPKMLDIWQEIFKKPNFSNSERMAMLLNNYCSS 185
           THI  +     +YEQG++ +SHCLD NLP M  +W EIF  P F + ER+  L+N   S 
Sbjct: 520 THICTNHTDPMKYEQGVMFSSHCLDKNLPHMFYLWGEIFGSPRFKDQERLRTLINMLASD 579

Query: 186 LTNGIVSSGHTYAVQAARSLISSVDECKENLLGIQHVTIMQEVQKTQKTEDIQSVIESIS 365
           L + I  SGH+YAV  A S ++      E L G+  VT      +  +   +Q       
Sbjct: 580 LASSIAQSGHSYAVSLASSSLTPAARLDEVLGGLSQVTYHHCCARL-RGHAVQVTYRHCC 638

Query: 366 ENVLKGNNLRAAFHYCNT 419
              L+G+ ++  +H+C T
Sbjct: 639 TR-LRGHAVQVTYHHCCT 655



 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 22/85 (25%), Positives = 39/85 (45%)
 Frame = +3

Query: 375  LKGNNLRAAFHYCNTNNDVHEYIDKFCKDLCNANDNQEVNRINWTDSKSMNKDNRGIHIA 554
            L+G+ ++  +H C T    H  +    +  C+A  +   +       K    ++R  H  
Sbjct: 769  LRGHAVQVTYHRCCTRLRGHA-VQTALEGFCDALPSSRSDTSGQVQDKEFTPESRRTHFM 827

Query: 555  MNIPVNFCAKVIPTVAYTHPDYAKL 629
            +  PVN+ ++ +  V Y H DYAKL
Sbjct: 828  LPYPVNYASRCVNAVTYNHEDYAKL 852


>SB_41612| Best HMM Match : OstA (HMM E-Value=5.2)
          Length = 442

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 25/75 (33%), Positives = 39/75 (52%), Gaps = 5/75 (6%)
 Frame = +3

Query: 363 SENVLKGN-NLRAAFHYC-NTNNDVHEYIDKFCK---DLCNANDNQEVNRINWTDSKSMN 527
           +++V   N +L  + H C NTNN V    +K      D+    +NQ VN    TD+K +N
Sbjct: 369 NQSVYSDNQSLDTSNHICVNTNNQVVNTNNKSVHTSHDISVDTNNQGVN----TDNKGVN 424

Query: 528 KDNRGIHIAMNIPVN 572
             N+G++   NI +N
Sbjct: 425 PYNQGLYPYNNICIN 439


>SB_27302| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 150

 Score = 29.5 bits (63), Expect = 2.7
 Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 5/67 (7%)
 Frame = +3

Query: 363 SENVLKGNNLRAAFHYCNTNNDVHEYIDKFCKDLCNANDNQ-----EVNRINWTDSKSMN 527
           S N    NN   + + CN NN ++   +    +  N N+N        NRIN + + + N
Sbjct: 79  SINNRNNNNNNNSINNCN-NNSINNRNNNSINNRSNTNNNSINNRSNTNRINSSSNNNDN 137

Query: 528 KDNRGIH 548
            DN+  H
Sbjct: 138 SDNKSKH 144


>SB_57619| Best HMM Match : DUF229 (HMM E-Value=0)
          Length = 616

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 16/65 (24%), Positives = 32/65 (49%)
 Frame = +3

Query: 69  HCLDHNLPKMLDIWQEIFKKPNFSNSERMAMLLNNYCSSLTNGIVSSGHTYAVQAARSLI 248
           H L +     +D+ Q +F++   S +  +A +  +YC+ +    VS GH +  Q A   +
Sbjct: 442 HILRYPDTPKVDVGQSLFQEVPKSRTCEVAGMPVHYCTCMDWKQVSPGHHHIKQLAEEAV 501

Query: 249 SSVDE 263
             ++E
Sbjct: 502 RHINE 506


>SB_12535| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 64

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = +3

Query: 420 NNDVHEYIDKFCKDLCNANDNQEVNRINWTDSKSMNKDNRGIH--IAMNIPVN 572
           NND  ++I+       N N+N   N  N  ++ + N +NR I+  IA+ I  N
Sbjct: 8   NNDNDKFINNSSNGNNNNNNNNNNNNNNNNNNNNNNNNNRNINAIIAITITTN 60


>SB_24400| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 813

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 18/65 (27%), Positives = 29/65 (44%)
 Frame = +3

Query: 342 QSVIESISENVLKGNNLRAAFHYCNTNNDVHEYIDKFCKDLCNANDNQEVNRINWTDSKS 521
           Q VI + ++N    NN  +     N NN       KFC+ + N ND   ++     ++  
Sbjct: 652 QQVINNNNDNSNNNNNSSSN----NNNNSSSNNNVKFCRCIKNNNDMNNIDTDTINNNSK 707

Query: 522 MNKDN 536
            NK+N
Sbjct: 708 SNKNN 712


>SB_32009| Best HMM Match : PKD (HMM E-Value=1.9e-19)
          Length = 3083

 Score = 28.3 bits (60), Expect = 6.3
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = -3

Query: 382  PLSTFSDMLSITDCISSVFCVFCTSCM 302
            P S+F+ +     C+S +FC   TSCM
Sbjct: 2461 PTSSFTRVQRCCCCLSLLFCTMVTSCM 2487


>SB_33997| Best HMM Match : Astacin (HMM E-Value=0)
          Length = 507

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 15/48 (31%), Positives = 21/48 (43%), Gaps = 1/48 (2%)
 Frame = +3

Query: 366 ENVLKGNN-LRAAFHYCNTNNDVHEYIDKFCKDLCNANDNQEVNRINW 506
           +N L  N+ L+A   YC TN   H  +D+ C     A      +  NW
Sbjct: 412 KNGLSPNDILQAKLMYCRTNGCTHTNLDQNCPGYAAAGYCTRTDLANW 459


>SB_32164| Best HMM Match : ECH (HMM E-Value=0.02)
          Length = 280

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 16/51 (31%), Positives = 25/51 (49%)
 Frame = +3

Query: 462 LCNANDNQEVNRINWTDSKSMNKDNRGIHIAMNIPVNFCAKVIPTVAYTHP 614
           LC     Q+  R+ +T++    K+N GI I MN P    A  +P +   +P
Sbjct: 69  LCADASEQKRTRVGYTEALLETKNNAGI-ITMNRPKALNALNLPMIRKIYP 118


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,220,039
Number of Sequences: 59808
Number of extensions: 489635
Number of successful extensions: 1518
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1327
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1510
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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