Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0221
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g28010.1 68417.m04018 pentatricopeptide (PPR) repeat-containi... 36 0.026
At5g09995.2 68418.m01155 expressed protein 34 0.10
At1g62670.1 68414.m07073 pentatricopeptide (PPR) repeat-containi... 31 0.73
At4g03120.1 68417.m00425 proline-rich family protein similar to ... 31 0.97
At1g13800.1 68414.m01620 pentatricopeptide (PPR) repeat-containi... 31 0.97
At3g13430.1 68416.m01688 zinc finger (C3HC4-type RING finger) fa... 30 1.3
At2g04300.1 68415.m00422 leucine-rich repeat protein kinase, put... 30 1.3
At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family pr... 30 1.7
At4g21820.1 68417.m03156 calmodulin-binding family protein conta... 29 2.2
At1g77210.1 68414.m08993 sugar transporter, putative similar to ... 29 3.9
At5g51270.1 68418.m06356 protein kinase family protein contains ... 28 5.2
At1g78450.1 68414.m09141 SOUL heme-binding family protein weak s... 28 5.2
At1g62930.1 68414.m07105 pentatricopeptide (PPR) repeat-containi... 28 5.2
At5g39450.1 68418.m04778 F-box family protein contains Pfam:PF00... 28 6.8
At5g04270.1 68418.m00419 zinc finger (DHHC type) family protein ... 27 9.0
>At4g28010.1 68417.m04018 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 704
Score = 35.9 bits (79), Expect = 0.026
Identities = 29/138 (21%), Positives = 64/138 (46%), Gaps = 8/138 (5%)
Frame = +3
Query: 12 ITEHVGQSGQYEQGILINSHCLDHNLPKMLDIWQEIFKKPNFSNSERMAMLLNNYCS--- 182
+ E +G + IL+NS ++ K +++W++I NS+ +++ +C
Sbjct: 450 LVEKLGAGDRVTTNILLNSTLKAGDVNKAMELWKQISDSKIVRNSDTYTAMIDGFCKTGM 509
Query: 183 -SLTNGIVSSGHTYAVQAA----RSLISSVDECKENLLGIQHVTIMQEVQKTQKTEDIQS 347
++ G++ +Q + L+SS+ CKE L Q + +E+Q+ D+ S
Sbjct: 510 LNVAKGLLCKMRVSELQPSVFDYNCLLSSL--CKEGSLD-QAWRLFEEMQRDNNFPDVVS 566
Query: 348 VIESISENVLKGNNLRAA 401
+ + LK ++++A
Sbjct: 567 -FNIMIDGSLKAGDIKSA 583
>At5g09995.2 68418.m01155 expressed protein
Length = 257
Score = 33.9 bits (74), Expect = 0.10
Identities = 28/112 (25%), Positives = 57/112 (50%), Gaps = 5/112 (4%)
Frame = +3
Query: 84 NLPKMLDIWQEIFKKPNFSNSERMA-MLLNNYCSSLTNGIVSSGHTYAVQAARSLISSVD 260
+L K+LD+ +E + P+ + R++ M +++ L++ + G T V+++ I +
Sbjct: 130 SLEKLLDVTRE--ELPDTMAAVRLSGMEISDLTMELSD--LGQGITQGVKSSTRAIRVAE 185
Query: 261 ECKENLLGIQHVTIMQEVQKTQKTEDIQSVI----ESISENVLKGNNLRAAF 404
+ L + V MQEV + KT++ + ++ S E V+KG +L F
Sbjct: 186 DRLRRLTNMNPVASMQEVMRQTKTDETEPMLAKQARSFREGVVKGRSLWQLF 237
>At1g62670.1 68414.m07073 pentatricopeptide (PPR) repeat-containing
protein contains multiple PPR repeats Pfam Profile:
PF01535
Length = 630
Score = 31.1 bits (67), Expect = 0.73
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = +3
Query: 42 YEQGILINSHCLDHNLPKMLDIWQEIFKKPNFSNSERMAMLLNNYCSS 185
Y ILIN C LP L + ++ K N ++ LLN YC S
Sbjct: 117 YTYSILINCFCRRSQLPLALAVLGKMMKLGYEPNIVTLSSLLNGYCHS 164
>At4g03120.1 68417.m00425 proline-rich family protein similar to U1
small nuclear ribonucleoprotein C; contains proline rich
extensin domains, INTERPRO:IPR002965
Length = 207
Score = 30.7 bits (66), Expect = 0.97
Identities = 14/31 (45%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +3
Query: 12 ITEHVGQSGQYEQ-GILINSHCLDHNLPKML 101
I EH+GQ+G Y+Q G + N H L P M+
Sbjct: 54 IKEHLGQTGGYQQVGAVFNQHMLARPRPPMM 84
>At1g13800.1 68414.m01620 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 883
Score = 30.7 bits (66), Expect = 0.97
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +3
Query: 42 YEQGILINSHCLDHNLPKMLDIWQEIFKKPNFSNSERMAMLLNNYC 179
Y +I H + N+PK +D++ ++ KK N ++ +L YC
Sbjct: 327 YVYSAIIEGHRKNMNIPKAVDVFNKMLKKRKRINCVIVSSILQCYC 372
>At3g13430.1 68416.m01688 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 315
Score = 30.3 bits (65), Expect = 1.3
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +3
Query: 342 QSVIESISENVLKGNNLRAAFHYCNTNNDVHEYIDKFCKDLC--NANDNQEVNRINWTDS 515
+SVI I + ++K N ++ F NND H+ D + N+N + + N DS
Sbjct: 16 RSVIPLIQDEIIKCNFCQSGFVEEMDNNDDHQAADSLWTPILMEMMNNNHDQHSTNQEDS 75
Query: 516 KSMNKD 533
+S+ +D
Sbjct: 76 ESILED 81
>At2g04300.1 68415.m00422 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 851
Score = 30.3 bits (65), Expect = 1.3
Identities = 25/85 (29%), Positives = 35/85 (41%), Gaps = 3/85 (3%)
Frame = +3
Query: 393 RAAFHYCNTNNDVHEYIDKFCKDL-CNANDNQEVNRINWTDSKSMNKDNRGIHIAMNIPV 569
R Y N ND H Y F +D N VN N D + I+ N P
Sbjct: 208 RRIIRYPNDVNDRHWY-PFFDEDAWTELTTNLNVNSSNGYDPPKFVMASASTPISKNAPF 266
Query: 570 NFCAKVIPTVA--YTHPDYAKLRVL 638
NF +IP+ A Y++ +A ++ L
Sbjct: 267 NFTWSLIPSTAKFYSYMHFADIQTL 291
>At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 258
Score = 29.9 bits (64), Expect = 1.7
Identities = 22/86 (25%), Positives = 38/86 (44%), Gaps = 3/86 (3%)
Frame = +3
Query: 84 NLPKMLDIWQEIFKKPNFSNSERMAMLLNNYCSSLTNGIVSS---GHTYAVQAARSLISS 254
NLP MLD W + S+ +R + + N C + +N V+ + Q S ++
Sbjct: 76 NLPAMLDSWDQSHHLQETSSLKRKLLDVENLCKTNSNCDVTRQELAKSKKKQRVSSESNT 135
Query: 255 VDECKENLLGIQHVTIMQEVQKTQKT 332
VDE N + Q ++ + +K T
Sbjct: 136 VDESNTNWVDGQSLSNSSDDEKASVT 161
>At4g21820.1 68417.m03156 calmodulin-binding family protein contains
IQ calmodulin-binding motif, Pfam:PF00612
Length = 1088
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/62 (25%), Positives = 32/62 (51%)
Frame = +3
Query: 339 IQSVIESISENVLKGNNLRAAFHYCNTNNDVHEYIDKFCKDLCNANDNQEVNRINWTDSK 518
I ++ ++SE +L N+ H C T +Y DK C++L A +++ + + S+
Sbjct: 893 INKLLSALSE-LLSMKNVHNILHICETLESATKYSDKCCEELVAAGAIEKLLTLIRSASR 951
Query: 519 SM 524
S+
Sbjct: 952 SI 953
>At1g77210.1 68414.m08993 sugar transporter, putative similar to
monosaccharide transporter PaMst-1 [Picea abies]
GI:2258137, sugar carrier protein GI:169735 from
[Ricinus communis], glucose transporter [Saccharum
hybrid cultivar H65-7052] GI:347855; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 504
Score = 28.7 bits (61), Expect = 3.9
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -3
Query: 406 WNAARKLFPLSTFSDMLSITDCISSVFCVFCTSCMIVT 293
W +LFPL T S S+ C++ F C +V+
Sbjct: 410 WLVPSELFPLETRSAGQSVVVCVNLFFTALIAQCFLVS 447
>At5g51270.1 68418.m06356 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 819
Score = 28.3 bits (60), Expect = 5.2
Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +3
Query: 333 EDIQSVIESISENVLKGNNLRAAFHYCNTNNDVHEYIDKFCKDLCNANDNQEVNRINWTD 512
++ + + S+S N GN + HY N D I K +L +A++ V ++ D
Sbjct: 313 DERKDAMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLD 372
Query: 513 -SKSMNK 530
S+ +N+
Sbjct: 373 ASRKLNE 379
>At1g78450.1 68414.m09141 SOUL heme-binding family protein weak
similarity to SOUL protein [Mus musculus] GI:4886906;
contains Pfam profile PF04832: SOUL heme-binding protein
Length = 225
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +3
Query: 351 IESISENVLKGNNLRAAFHYCNTNNDVHEYID 446
I+ IS N GN Y N NND H+ I+
Sbjct: 64 IQDISLNEASGNGWNQLSDYMNGNNDYHQRIE 95
>At1g62930.1 68414.m07105 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 659
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +3
Query: 42 YEQGILINSHCLDHNLPKMLDIWQEIFKKPNFSNSERMAMLLNNYC 179
Y ILIN C LP L + ++ K + ++ LLN YC
Sbjct: 116 YSYNILINCFCRRSQLPLALAVLGKMMKLGYEPDIVTLSSLLNGYC 161
>At5g39450.1 68418.m04778 F-box family protein contains Pfam:PF00646
F-box domain ; similar to SKP1 interacting partner 2
(SKIP2) TIGR_Ath1:At5g67250
Length = 579
Score = 27.9 bits (59), Expect = 6.8
Identities = 9/28 (32%), Positives = 17/28 (60%)
Frame = +2
Query: 578 RQSHTDCCVHPSGLREATRAVSVLIVEI 661
R CC+HP +R ++ +VL++E+
Sbjct: 165 RDGKVQCCLHPGFVRGIEKSCNVLLLEV 192
>At5g04270.1 68418.m00419 zinc finger (DHHC type) family protein low
similarity to Golgi-specific DHHC zinc figer protein
[Mus musculus] GI:21728103; contains Pfam profile
PF01529: DHHC zinc finger domain
Length = 284
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 3/57 (5%)
Frame = -1
Query: 498 CD*LLDYH*RYTGPCRIYR-CI--RGHRCLYCNNGMLLANYSL*VRFLICSLLPTVY 337
CD Y T CR+ R C+ H CL+ NN + ANY + + + ++Y
Sbjct: 107 CDKCFAYKPLRTHHCRVCRRCVLKMDHHCLWINNCVGYANYKAFFILVFYATVASIY 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,678,886
Number of Sequences: 28952
Number of extensions: 332476
Number of successful extensions: 1084
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1084
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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