BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0219 (668 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 357 3e-99 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 357 3e-99 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 357 3e-99 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 357 3e-99 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 198 4e-51 At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 197 5e-51 At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 102 2e-22 At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 99 3e-21 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 2e-08 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 2e-08 At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 52 3e-07 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 3e-07 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 5e-07 At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 48 6e-06 At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 43 2e-04 At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 43 2e-04 At5g13650.2 68418.m01585 elongation factor family protein contai... 42 3e-04 At5g13650.1 68418.m01584 elongation factor family protein contai... 42 3e-04 At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 41 9e-04 At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 40 0.001 At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.014 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.014 At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 36 0.032 At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 33 0.23 At5g46180.1 68418.m05680 ornithine aminotransferase, putative / ... 30 1.2 At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.2 At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.2 At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.6 At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.6 At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.1 At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 4.9 At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase fa... 28 6.5 At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 8.5 At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 8.5 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 357 bits (879), Expect = 3e-99 Identities = 168/207 (81%), Positives = 184/207 (88%) Frame = +1 Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 576 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 577 IGYNPXAVAFVPISGWHGDNMLEPSPN 657 +GYNP + FVPISG+ GDNM+E S N Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTN 207 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 357 bits (879), Expect = 3e-99 Identities = 168/207 (81%), Positives = 184/207 (88%) Frame = +1 Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 576 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 577 IGYNPXAVAFVPISGWHGDNMLEPSPN 657 +GYNP + FVPISG+ GDNM+E S N Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTN 207 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 357 bits (879), Expect = 3e-99 Identities = 168/207 (81%), Positives = 184/207 (88%) Frame = +1 Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 576 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 577 IGYNPXAVAFVPISGWHGDNMLEPSPN 657 +GYNP + FVPISG+ GDNM+E S N Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTN 207 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 357 bits (879), Expect = 3e-99 Identities = 168/207 (81%), Positives = 184/207 (88%) Frame = +1 Query: 37 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 216 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 217 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 396 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 397 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 576 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 577 IGYNPXAVAFVPISGWHGDNMLEPSPN 657 +GYNP + FVPISG+ GDNM+E S N Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTN 207 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 198 bits (482), Expect = 4e-51 Identities = 91/206 (44%), Positives = 139/206 (67%), Gaps = 2/206 (0%) Frame = +1 Query: 52 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 231 + +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+ Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297 Query: 232 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 411 ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357 Query: 412 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 588 G GQTREHA + GV+Q+IV +NKMD YS+ RF+ IK+ V S+++ + Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFK 415 Query: 589 PXAVAFVPISGWHGDNML-EPSPNAL 663 ++ ++P+S N++ PS N L Sbjct: 416 DSSLTWIPLSAMENQNLVAAPSDNRL 441 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 197 bits (481), Expect = 5e-51 Identities = 93/199 (46%), Positives = 136/199 (68%), Gaps = 1/199 (0%) Frame = +1 Query: 46 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 225 +K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157 Query: 226 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 405 + ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217 Query: 406 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGY 585 E G + GQTREH LA TLGV +LIV VNKMD +S+ R++EI++++ ++K GY Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGY 277 Query: 586 NPXA-VAFVPISGWHGDNM 639 N V F+PISG G NM Sbjct: 278 NTKKDVVFLPISGLMGKNM 296 >At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) identical to SWISS-PROT:P17745 elongation factor Tu, chloroplast precursor (EF-Tu) [Arabidopsis thaliana] Length = 476 Score = 102 bits (245), Expect = 2e-22 Identities = 63/179 (35%), Positives = 93/179 (51%), Gaps = 1/179 (0%) Frame = +1 Query: 34 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 213 K ++K H+NI IGHVD GK+T T L I +K+++ Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116 Query: 214 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 393 +D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176 Query: 394 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE-EIKKEVSSY 567 G QT+EH LLA +GV ++V +NK D + E E+++ +SSY Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228 >At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu, putative similar to mitochondrial elongation factor Tu [Arabidopsis thaliana] gi|1149571|emb|CAA61511 Length = 454 Score = 98.7 bits (235), Expect = 3e-21 Identities = 66/198 (33%), Positives = 97/198 (48%) Frame = +1 Query: 43 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 222 + K H+N+ IGHVD GK+T T + K E GK +DK Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107 Query: 223 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 402 E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167 Query: 403 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 582 QT+EH LLA +GV L+ +NK+D + P E +E+ S+ K G Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYKFPG 220 Query: 583 YNPXAVAFVPISGWHGDN 636 + + +S G N Sbjct: 221 DDIPIIRGSALSALQGTN 238 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 56.4 bits (130), Expect = 2e-08 Identities = 41/128 (32%), Positives = 61/128 (47%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116 Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420 +GITI A Y V IID PGH DF + D A+L++ + G I+ Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176 Query: 421 KNGQTREH 444 + Q R + Sbjct: 177 VDRQMRRY 184 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 56.4 bits (130), Expect = 2e-08 Identities = 41/128 (32%), Positives = 61/128 (47%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116 Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420 +GITI A Y V IID PGH DF + D A+L++ + G I+ Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176 Query: 421 KNGQTREH 444 + Q R + Sbjct: 177 VDRQMRRY 184 >At5g08650.1 68418.m01029 GTP-binding protein LepA, putative Length = 681 Score = 52.4 bits (120), Expect = 3e-07 Identities = 52/174 (29%), Positives = 81/174 (46%), Gaps = 4/174 (2%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 N +I H+D GKST L+ G + R + K F LD + ERE Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131 Query: 241 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 408 RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190 Query: 409 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 570 A QT + LA + ++I +NK+D P +EP E++ +E+ I Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVI 232 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 52.0 bits (119), Expect = 3e-07 Identities = 36/113 (31%), Positives = 53/113 (46%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 NI ++ HVD GK+T HLI GG + GK F +D L E+ Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57 Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399 R IT+ + + Y + +ID+PGH DF + T +D A+++V A G Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 51.6 bits (118), Expect = 5e-07 Identities = 36/117 (30%), Positives = 56/117 (47%) Frame = +1 Query: 49 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 228 K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++ Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140 Query: 229 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399 E+ERGITI A K+ + IID PGH DF + D A+ + + G Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197 >At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-binding protein GUF1 - Saccharomyces cerevisiae, PIR:S50374 Length = 661 Score = 48.0 bits (109), Expect = 6e-06 Identities = 58/189 (30%), Positives = 80/189 (42%), Gaps = 9/189 (4%) Frame = +1 Query: 25 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 204 D K EK N +I H+D GKST L+ G I K G G +Y Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102 Query: 205 AWVLDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTS 357 LDKL +RERGIT+ E S Y + +ID PGH DF + S Sbjct: 103 ---LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLS 157 Query: 358 QADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 537 A+L+V A G QT + LAF + ++ +NK+D P ++P Sbjct: 158 ACQGALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKIDQ---PTADP-- 204 Query: 538 EEIKKEVSS 564 E +K ++ S Sbjct: 205 ERVKAQLKS 213 >At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to SP|Q09130 Eukaryotic translation initiation factor 2 gamma subunit (eIF-2- gamma) {Schizosaccharomyces pombe}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain; isoform predicted to contain a TG non-consensus acceptor splice site. Length = 471 Score = 42.7 bits (96), Expect = 2e-04 Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%) Frame = +1 Query: 31 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 201 P++ + INI IGHV GKST ++ G+ R + E+ ++G + K Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86 Query: 202 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 348 Y DK + +E T D+ ++ T + +V+ +D PGH + M+ Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146 Query: 349 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 528 G + D A+L++AA QT EH + +K +I+ NK+D + Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200 Query: 529 PRFEEIKKEVSS 564 + E I+K +++ Sbjct: 201 EQHEAIQKFITN 212 >At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to SP|Q09130 Eukaryotic translation initiation factor 2 gamma subunit (eIF-2- gamma) {Schizosaccharomyces pombe}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain; isoform predicted to contain a TG non-consensus acceptor splice site. Length = 284 Score = 42.7 bits (96), Expect = 2e-04 Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%) Frame = +1 Query: 31 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 201 P++ + INI IGHV GKST ++ G+ R + E+ ++G + K Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86 Query: 202 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 348 Y DK + +E T D+ ++ T + +V+ +D PGH + M+ Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146 Query: 349 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 528 G + D A+L++AA QT EH + +K +I+ NK+D + Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200 Query: 529 PRFEEIKKEVSS 564 + E I+K +++ Sbjct: 201 EQHEAIQKFITN 212 >At5g13650.2 68418.m01585 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 676 Score = 42.3 bits (95), Expect = 3e-04 Identities = 46/159 (28%), Positives = 66/159 (41%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129 Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420 RGITI V IID PGH DF + + D +L+V + G Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185 Query: 421 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 537 QTR A G ++V VNK+D P + P F Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217 >At5g13650.1 68418.m01584 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 675 Score = 42.3 bits (95), Expect = 3e-04 Identities = 46/159 (28%), Positives = 66/159 (41%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128 Query: 241 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 420 RGITI V IID PGH DF + + D +L+V + G Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184 Query: 421 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 537 QTR A G ++V VNK+D P + P F Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216 >At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative Length = 465 Score = 40.7 bits (91), Expect = 9e-04 Identities = 44/186 (23%), Positives = 82/186 (44%), Gaps = 8/186 (4%) Frame = +1 Query: 31 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 192 P++ + INI IGHV GKST +G H + +++ TI+ A+ Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84 Query: 193 SFKYAWVLDKLKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 366 + +E + D++ + K +V+ +D PGH + M+ G + D Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144 Query: 367 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 546 A+LI+AA QT EH + +K +I+ NK+D + + + E+I Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKIDLIQENEAIKQHEDI 198 Query: 547 KKEVSS 564 ++ +++ Sbjct: 199 QRFITN 204 >At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to gb|U37354 from S. pombe. ESTs gb|T41979, gb|N37284 and gb|N37529 come from this gene Length = 465 Score = 40.3 bits (90), Expect = 0.001 Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%) Frame = +1 Query: 274 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 441 FE SK +V+ +D PGH + M+ G + D A+L++AA QT E Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165 Query: 442 HALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 570 H + +K +I+ NK+D + + + E I+K + + + Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 39.1 bits (87), Expect = 0.003 Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 228 N+ VI HVD GKST T L+ G I + + + A E +G + K + + Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80 Query: 229 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 384 E ++ + ++Y + +ID+PGH DF + D A+++V Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 36.7 bits (81), Expect = 0.014 Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 N+ ++GH+ GK+ L+ + + K EK KY D E+E Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187 Query: 241 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399 R I+I + L + Y I+D PGH +F M AD AVLIV A G Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 36.7 bits (81), Expect = 0.014 Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%) Frame = +1 Query: 61 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 240 N+ ++GH+ GK+ L+ + + K EK KY D E+E Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187 Query: 241 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 399 R I+I + L + Y I+D PGH +F M AD AVLIV A G Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245 >At4g11160.1 68417.m01808 translation initiation factor IF-2, mitochondrial, putative similar to SP|P46198|IF2M_BOVIN Translation initiation factor IF-2, mitochondrial precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus} Length = 743 Score = 35.5 bits (78), Expect = 0.032 Identities = 33/105 (31%), Positives = 50/105 (47%), Gaps = 3/105 (2%) Frame = +1 Query: 295 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 474 +T +D PGH F + G + D VL+VAA G QT E A + V Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321 Query: 475 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSS---YIKKIGYNPXAV 600 ++V +NK D P + P E++K +++S ++ IG N AV Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTSEGIELEDIGGNVQAV 361 >At3g01360.1 68416.m00057 expressed protein contains Pfam profile PF04819: Family of unknown function (DUF716) (Plant viral-response family) Length = 319 Score = 32.7 bits (71), Expect = 0.23 Identities = 27/96 (28%), Positives = 44/96 (45%), Gaps = 1/96 (1%) Frame = +1 Query: 304 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 480 I GH D+ + T Q +C + L+V TG F +KNG R+ LG + Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285 Query: 481 IVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 588 + +DS E +E+++E S+ K++G N Sbjct: 286 NLSTFTLDSDEE-------DEVREE-SNVAKEVGLN 313 >At5g46180.1 68418.m05680 ornithine aminotransferase, putative / ornithine--oxo-acid aminotransferase, putative similar to SP|Q92413 Ornithine aminotransferase (EC 2.6.1.13) (Ornithine--oxo-acid aminotransferase) [Aspergillus nidulans] {Emericella nidulans}; contains Pfam profile PF00202: aminotransferase, class III Length = 475 Score = 30.3 bits (65), Expect = 1.2 Identities = 17/54 (31%), Positives = 28/54 (51%) Frame = +1 Query: 460 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPISG 621 T GV++ G+ + +S PP S R E++ E S++ Y+P V F +G Sbjct: 18 TAGVRRSYGGLPQSNSKSPPSSSQRLMELESEFSAH----NYHPVPVVFSRANG 67 >At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1201 Score = 30.3 bits (65), Expect = 1.2 Identities = 21/68 (30%), Positives = 34/68 (50%) Frame = +1 Query: 301 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 480 +ID PGH F G+S D A+L+V + + G+ QT E +L + + Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756 Query: 481 IVGVNKMD 504 I+ +NK+D Sbjct: 757 IIALNKVD 764 >At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1088 Score = 30.3 bits (65), Expect = 1.2 Identities = 22/70 (31%), Positives = 33/70 (47%) Frame = +1 Query: 295 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 474 + +ID PGH F G++ D A+L+V + G+ QT E L VK Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610 Query: 475 QLIVGVNKMD 504 I+ +NK+D Sbjct: 611 -FIIALNKVD 619 >At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain Length = 630 Score = 29.9 bits (64), Expect = 1.6 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +1 Query: 301 IIDAPGHRDFIKNMITGTSQADCAVLIV 384 +ID PGH F G+S D A+L+V Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140 >At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to IF2 protein [Drosophila melanogaster] GI:7108770; contains Pfam profile PF03144: Elongation factor Tu domain 2 Length = 1294 Score = 29.9 bits (64), Expect = 1.6 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +1 Query: 301 IIDAPGHRDFIKNMITGTSQADCAVLIV 384 +ID PGH F G+S D A+L+V Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799 >At1g17220.1 68414.m02098 translation initiation factor IF-2, chloroplast, putative similar to SP|P57997|IF2C_PHAVU Translation initiation factor IF-2, chloroplast precursor (PvIF2cp) {Phaseolus vulgaris} Length = 1026 Score = 29.5 bits (63), Expect = 2.1 Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%) Frame = +1 Query: 220 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 387 K+ A GIT I +K +D PGH F G D A+++VA Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584 Query: 388 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 504 A G QT E A+ +++ +NK+D Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615 >At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein similar to 1,4-alpha-glucan branching enzyme [Solanum tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum tuberosum} SP|P30924; contains Pfam profiles: PF00128 Alpha amylase catalytic domain, PF02922 Isoamylase N-terminal domain Length = 777 Score = 28.3 bits (60), Expect = 4.9 Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%) Frame = +1 Query: 523 SEPR---FEEIKKEVSSYIKKIGYNPXAVAFVP 612 SEP+ FEE K+V ++K+ GYN + VP Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289 >At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase family protein similar to Potential phospholipid-transporting ATPase (EC 3.6.3.1) from Homo sapiens [SP|Q9Y2Q0, SP|O43520], Mus musculus [SP|P98200, SP|P70704], {Bos taurus} SP|Q29449; contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase Length = 1184 Score = 27.9 bits (59), Expect = 6.5 Identities = 15/53 (28%), Positives = 24/53 (45%) Frame = +1 Query: 406 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 564 E+G +TR+H G++ LI+ ++D E R E K VS+ Sbjct: 629 ESGRKYEKETRDHVNEYADAGLRTLILAYRELDENEYEVFTERISEAKNSVSA 681 >At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329: Protein of unknown function (DUF731) Length = 442 Score = 27.5 bits (58), Expect = 8.5 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +1 Query: 505 STEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPIS 618 +TE + P+ E K E+S++I IG+ V F P S Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100 >At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329: Protein of unknown function (DUF731) Length = 440 Score = 27.5 bits (58), Expect = 8.5 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +1 Query: 505 STEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPIS 618 +TE + P+ E K E+S++I IG+ V F P S Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,636,004 Number of Sequences: 28952 Number of extensions: 300570 Number of successful extensions: 942 Number of sequences better than 10.0: 35 Number of HSP's better than 10.0 without gapping: 896 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 926 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1412971776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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