BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0205
(737 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 28 1.2
SPBC216.06c |swi1||replication fork protection complex subunit S... 28 1.2
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 28 1.6
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 26 4.9
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 26 6.4
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 8.5
>SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 871
Score = 28.3 bits (60), Expect = 1.2
Identities = 32/102 (31%), Positives = 45/102 (44%), Gaps = 12/102 (11%)
Frame = +1
Query: 232 EEIKSTGNWNTEECKEYIK-----PLDKSF-------EILYGFPLNQILYINIFDKKDIF 375
E+I++ NW+TEE E++ + +F EIL G N + +NI F
Sbjct: 242 EKIQNVPNWSTEEVVEWLMNAGLGSVAPNFAENEITGEILLGLDSNVLKELNITSFGKRF 301
Query: 376 NVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQKS 501
V KI K DS ++ SL E Y E + VS S S
Sbjct: 302 EVLRKIQQLK---DSYEQ--SLYEEYPQFAEPISVSQSSDSS 338
>SPBC216.06c |swi1||replication fork protection complex subunit
Swi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 971
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/54 (25%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = -1
Query: 395 RIL-MHTLKISFLSKIFIYKI*FNGKPYKISKDLSKGFMYSLHSSVFQLPVLFI 237
R+L +H +++ ++ F+ + F+ KP K + S + + L +SVF L +
Sbjct: 362 RVLPIHKMQLLYVQSFFLEFMRFSSKPKKTEEIYSNDYSFGLAASVFDQRALIM 415
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 27.9 bits (59), Expect = 1.6
Identities = 19/65 (29%), Positives = 32/65 (49%)
Frame = +1
Query: 370 IFNVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQKSLYCICFIEYANEDINV 549
I ++ ++ E + DSLKK L+E Y K+ S++ Y ++ +DINV
Sbjct: 1104 IIDISDELQDESSSRDSLKKGIELIEDYYLSQSTSKLESSVEGKNY---LLKVKFKDINV 1160
Query: 550 N*SIH 564
N +H
Sbjct: 1161 NWDLH 1165
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 26.2 bits (55), Expect = 4.9
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
Frame = +1
Query: 226 LYEEIK---STGNWNTEECKEYIKPLDKSFEILYGFPLNQ 336
L +EIK G W+ E+ ++++ L+K + +Y F N+
Sbjct: 25 LKKEIKRRNDQGGWSEEDESDFVELLEKELDKVYSFQKNK 64
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 25.8 bits (54), Expect = 6.4
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 84 YSFRKFW*QALMNLID*VCVFIHIY 10
+ FR+FW L +L+ V +F HIY
Sbjct: 41 FHFRRFWTIWLKSLVIMVLLFTHIY 65
>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1854
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +3
Query: 465 RKSKGKYINSKISLLYLFY*VCQ*RYKCKLKYSYFRNMMLMLIRVQY 605
R G YI + LL +FY C + Y+Y RN + +++ + Y
Sbjct: 1272 RTFTGLYIVHLLFLLTIFY-------PCPIAYTYVRNSIFLILSICY 1311
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,781,077
Number of Sequences: 5004
Number of extensions: 54094
Number of successful extensions: 138
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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