Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0205
(737 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g01140.3 68414.m00020 CBL-interacting protein kinase 9 (CIPK9... 30 1.8
At1g01140.2 68414.m00019 CBL-interacting protein kinase 9 (CIPK9... 30 1.8
At1g01140.1 68414.m00018 CBL-interacting protein kinase 9 (CIPK9... 30 1.8
At5g07070.1 68418.m00800 CBL-interacting protein kinase 2 (CIPK2... 29 2.4
At5g27320.1 68418.m03262 expressed protein similar to PrMC3 [Pin... 28 7.4
At5g17410.2 68418.m02043 tubulin family protein similar to spind... 27 9.8
At5g17410.1 68418.m02042 tubulin family protein similar to spind... 27 9.8
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 27 9.8
>At1g01140.3 68414.m00020 CBL-interacting protein kinase 9 (CIPK9)
identical to CBL-interacting protein kinase 9
[Arabidopsis thaliana] gi|13249117|gb|AAK16684; contains
Pfam profiles PF00069: Protein kinase domain and
PF03822: NAF domain; identical to cDNA CBL-interacting
protein kinase 9 (CIPK9) GI:13249116
Length = 451
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/51 (27%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 346 INIFDKKDIF-NVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQ 495
I I D++ +F + ++ L +I+ L K+P++VE+ + K K+ I ++
Sbjct: 47 IKILDREKVFRHKMVEQLKREISTMKLIKHPNVVEIIEVMASKTKIYIVLE 97
>At1g01140.2 68414.m00019 CBL-interacting protein kinase 9 (CIPK9)
identical to CBL-interacting protein kinase 9
[Arabidopsis thaliana] gi|13249117|gb|AAK16684; contains
Pfam profiles PF00069: Protein kinase domain and
PF03822: NAF domain; identical to cDNA CBL-interacting
protein kinase 9 (CIPK9) GI:13249116
Length = 449
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/51 (27%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 346 INIFDKKDIF-NVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQ 495
I I D++ +F + ++ L +I+ L K+P++VE+ + K K+ I ++
Sbjct: 47 IKILDREKVFRHKMVEQLKREISTMKLIKHPNVVEIIEVMASKTKIYIVLE 97
>At1g01140.1 68414.m00018 CBL-interacting protein kinase 9 (CIPK9)
identical to CBL-interacting protein kinase 9
[Arabidopsis thaliana] gi|13249117|gb|AAK16684; contains
Pfam profiles PF00069: Protein kinase domain and
PF03822: NAF domain; identical to cDNA CBL-interacting
protein kinase 9 (CIPK9) GI:13249116
Length = 447
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/51 (27%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 346 INIFDKKDIF-NVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQ 495
I I D++ +F + ++ L +I+ L K+P++VE+ + K K+ I ++
Sbjct: 47 IKILDREKVFRHKMVEQLKREISTMKLIKHPNVVEIIEVMASKTKIYIVLE 97
>At5g07070.1 68418.m00800 CBL-interacting protein kinase 2 (CIPK2)
identical to CBL-interacting protein kinase 2
[Arabidopsis thaliana] gi|9280636|gb|AAF86506
Length = 456
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/61 (26%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +1
Query: 331 NQILYINIFDKKDIFNVCI-KILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQKSLY 507
N+ + I + DK + V + + + +I+V + K+P++VE+Y + K ++ I+ Y
Sbjct: 35 NESVAIKMIDKDKVMRVGLSQQIKREISVMRIAKHPNVVELYEVMATKSRIYFVIE---Y 91
Query: 508 C 510
C
Sbjct: 92 C 92
>At5g27320.1 68418.m03262 expressed protein similar to PrMC3 [Pinus
radiata] GI:5487873
Length = 344
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/38 (31%), Positives = 24/38 (63%)
Frame = +1
Query: 4 FLVNMDKNTNLINQIHKCLLPKLSKGIDETENPIEYEI 117
F V +D+ TNL++++++ S I + +NP++ EI
Sbjct: 66 FDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEI 103
>At5g17410.2 68418.m02043 tubulin family protein similar to spindle
pole body protein [Homo
sapiens][GI:2801701][PMID:9566967], gamma-tubulin ring
protein Dgrip84 [Drosophila
melanogaster][GI:4689225][PMID: 10037793]
Length = 679
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/74 (18%), Positives = 35/74 (47%)
Frame = +1
Query: 325 PLNQILYINIFDKKDIFNVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQKSL 504
P+++ + IF + CIK HE +++ + ++ L +K + + +
Sbjct: 322 PISERSKLTIFGSNHHYLECIKAAHEFASIELVNLIKDKYDLVGRL-RSIKHYLLLDQGD 380
Query: 505 YCICFIEYANEDIN 546
+ + F++ A E++N
Sbjct: 381 FLVHFMDIAREELN 394
>At5g17410.1 68418.m02042 tubulin family protein similar to spindle
pole body protein [Homo
sapiens][GI:2801701][PMID:9566967], gamma-tubulin ring
protein Dgrip84 [Drosophila
melanogaster][GI:4689225][PMID: 10037793]
Length = 678
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/74 (18%), Positives = 35/74 (47%)
Frame = +1
Query: 325 PLNQILYINIFDKKDIFNVCIKILHEKITVDSLKKYPSLVEVYSSLVEKVKVSISIQKSL 504
P+++ + IF + CIK HE +++ + ++ L +K + + +
Sbjct: 321 PISERSKLTIFGSNHHYLECIKAAHEFASIELVNLIKDKYDLVGRL-RSIKHYLLLDQGD 379
Query: 505 YCICFIEYANEDIN 546
+ + F++ A E++N
Sbjct: 380 FLVHFMDIAREELN 393
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 27.5 bits (58), Expect = 9.8
Identities = 18/79 (22%), Positives = 34/79 (43%)
Frame = +1
Query: 208 LSNIIILYEEIKSTGNWNTEECKEYIKPLDKSFEILYGFPLNQILYINIFDKKDIFNVCI 387
L+ + I E+IK T CK+ ++ + F+ G L +L K+D +C
Sbjct: 629 LAGLKISLEDIKDTDRIMYNSCKQILEMDPEFFDSNAGLGLTFVLETEELGKRDTIELCP 688
Query: 388 KILHEKITVDSLKKYPSLV 444
+ + + K+Y L+
Sbjct: 689 DGKLKAVNSKNRKQYVDLL 707
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,141,829
Number of Sequences: 28952
Number of extensions: 233484
Number of successful extensions: 556
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 548
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 556
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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