BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0200 (659 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 32 0.39 At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 29 2.1 At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 3.6 At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 29 3.6 At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 26 4.2 At3g49990.1 68416.m05466 expressed protein 28 4.8 At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containi... 28 4.8 At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein ... 27 8.4 >At5g26200.1 68418.m03118 mitochondrial substrate carrier family protein contains Pfam profile: PF00153 mitochondrial carrier protein Length = 342 Score = 31.9 bits (69), Expect = 0.39 Identities = 17/35 (48%), Positives = 24/35 (68%) Frame = +2 Query: 314 LTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 418 +T+SS+ AT R+G + TTS + A G A LT+A A Sbjct: 112 ITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146 >At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related contains weak similarity to vacuolar calcium binding protein [Raphanus sativus] gi|9049359|dbj|BAA99394 Length = 152 Score = 29.5 bits (63), Expect = 2.1 Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 2/83 (2%) Frame = -3 Query: 249 EVPAIPEAKKDDIAPEDSDIAXXXXXXXXXXXXXXXEAKSSEIPDAEAKSADIKV-EEPA 73 E P + E KKD+ AP ++ + K E+ + + + A + V EE Sbjct: 73 ETPEVVEIKKDEEAPVETPVV---VEDESKTEEVVEAKKEEEVEEKKTEEAPVVVEEEKK 129 Query: 72 AQPEDSKTEVQATV-AEISKEEK 7 + E+ K V+A+V A + K ++ Sbjct: 130 PEAEEEKPAVEASVTAPVEKADE 152 >At2g45800.1 68415.m05696 LIM domain-containing protein similar to PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain protein PLIM1 [Nicotiana tabacum] GI:5932418; contains Pfam profile PF00412: LIM domain Length = 226 Score = 28.7 bits (61), Expect = 3.6 Identities = 11/17 (64%), Positives = 14/17 (82%) Frame = -3 Query: 252 DEVPAIPEAKKDDIAPE 202 D+ +IPEAK+DD APE Sbjct: 197 DDTASIPEAKEDDAAPE 213 >At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus; similar to SEC14-like protein 2 (Alpha-tocopherol associated protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos taurus} Length = 683 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = -3 Query: 120 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEK 7 P E K + K EE + E+ KTE ++ AE +EEK Sbjct: 123 PVKEEKVEEKKTEETEEKKEEVKTEEKSLEAETKEEEK 160 >At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2 (CPK2) identical to calcium-dependent protein kinase isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 646 Score = 26.2 bits (55), Expect(2) = 4.2 Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = -3 Query: 126 EIPDAEAKSADIKVEEPAAQPED-SKTEVQATVAEISKEEKP 4 E+P E+K ++ V+ +A+PE S+++ + T E + E KP Sbjct: 109 EVPPEESKR-EVVVQPESAKPETKSESKPETTKPETTSETKP 149 Score = 20.6 bits (41), Expect(2) = 4.2 Identities = 6/14 (42%), Positives = 10/14 (71%) Frame = -3 Query: 237 IPEAKKDDIAPEDS 196 +PE K ++ PE+S Sbjct: 102 VPEESKQEVPPEES 115 >At3g49990.1 68416.m05466 expressed protein Length = 502 Score = 28.3 bits (60), Expect = 4.8 Identities = 15/39 (38%), Positives = 20/39 (51%) Frame = -3 Query: 123 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEK 7 +P A+ D+K E P A+P KT Q + E KE K Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEE-KKERK 459 >At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containing protein low similarity to CRP1 [Zea mays] GI:3289002; contains Pfam profile PF01535: PPR repeat Length = 793 Score = 28.3 bits (60), Expect = 4.8 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = -1 Query: 116 MLKQNLLILKWKNQLLSLKIQKLKYKLPSLKFQKKKNL 3 M K+N+ + K+K ++L LK K YK + K Q + L Sbjct: 740 MEKKNMFVDKYKYRMLFLKYHKTAYKGKAPKVQSESQL 777 >At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein contains Pfam profile: PF00096 zinc finger, C2H2 type Length = 586 Score = 27.5 bits (58), Expect = 8.4 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = +3 Query: 150 FLRFSLQERFPVSRYRSLLGLYRPFWPLV 236 FL F E PV ++ L L RP WPL+ Sbjct: 25 FLSFPRPENTPVQLFQLLHTLERPGWPLL 53 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,637,263 Number of Sequences: 28952 Number of extensions: 197923 Number of successful extensions: 471 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 461 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 471 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1383534864 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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