Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0194
(424 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58070.1 68418.m07267 lipocalin, putative similar to temperat... 46 8e-06
At5g61780.1 68418.m07753 tudor domain-containing protein / nucle... 34 0.046
At5g37190.1 68418.m04465 COP1-interacting protein 4 (CIP4) simil... 30 0.56
At4g00930.1 68417.m00126 COP1-interacting protein 4.1 (CIP4.1) i... 29 1.7
At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor, pu... 28 2.3
At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 28 3.0
At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containi... 28 3.0
At2g29070.2 68415.m03533 ubiquitin fusion degradation UFD1 famil... 27 4.0
At2g29070.1 68415.m03534 ubiquitin fusion degradation UFD1 famil... 27 4.0
At5g36740.1 68418.m04402 PHD finger family protein 27 5.2
At5g36670.1 68418.m04388 PHD finger family protein 27 5.2
At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative s... 27 6.9
At5g37100.1 68418.m04453 replication protein-related weak simila... 27 6.9
At3g05150.1 68416.m00559 sugar transporter family protein simila... 27 6.9
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 26 9.1
At1g59560.1 68414.m06698 expressed protein contains similarity t... 26 9.1
At1g08890.1 68414.m00989 sugar transporter family protein simila... 26 9.1
>At5g58070.1 68418.m07267 lipocalin, putative similar to temperature
stress-induced lipocalin [Triticum aestivum] GI:18650668
Length = 186
Score = 46.4 bits (105), Expect = 8e-06
Identities = 28/82 (34%), Positives = 41/82 (50%), Gaps = 2/82 (2%)
Frame = +2
Query: 104 ELKPXNNFNLTAYQGIWYEISKFPNESE-KNGKCSSXEYKLEGD-VVKVKNVHIIDGVKK 277
E++ N+ Y G WYEI+ FP+ + KNG + Y L D + V N +G +
Sbjct: 6 EMEVVKGLNVERYMGRWYEIASFPSRFQPKNGVDTRATYTLNPDGTIHVLNETWSNGKRG 65
Query: 278 YIEWTAKLTDDANKAAKLTVTF 343
+IE +A D + AKL V F
Sbjct: 66 FIEGSAYKADPKSDEAKLKVKF 87
>At5g61780.1 68418.m07753 tudor domain-containing protein / nuclease
family protein contains Pfam domains PF00567: Tudor
domain and PF00565: Staphylococcal nuclease homologue
Length = 985
Score = 33.9 bits (74), Expect = 0.046
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = +2
Query: 215 YKLEGDVVKVKNVHIID-GVKKYIEWTAKLTD-DANKAAKLT 334
Y +GD VK + +++ G+ KY+EW+A + D +A K K T
Sbjct: 313 YYSDGDTVKDLGLELVENGLAKYVEWSANMLDEEAKKKLKAT 354
>At5g37190.1 68418.m04465 COP1-interacting protein 4 (CIP4) similar
to COP1-interacting protein 4 (CIP4) [Arabidopsis
thaliana] GI:13160646; supporting cDNA
gi|13160645|dbj|AB036832.1|;
Length = 876
Score = 30.3 bits (65), Expect = 0.56
Identities = 21/75 (28%), Positives = 36/75 (48%)
Frame = +2
Query: 185 EKNGKCSSXEYKLEGDVVKVKNVHIIDGVKKYIEWTAKLTDDANKAAKLTVTFKFGEISR 364
EK+GK SS K + + V+ ++D +++ E L K++K T K ++
Sbjct: 515 EKSGKKSSKRSKKKDSLNIVEEAQVVDSLQQKKEAEENLEKSGKKSSKKTK--KKDSLNI 572
Query: 365 DGSVQVLATDYNNYA 409
QVL+ + NN A
Sbjct: 573 VEEAQVLSVEVNNVA 587
>At4g00930.1 68417.m00126 COP1-interacting protein 4.1 (CIP4.1)
identical to cDNA CIP4.1 mRNA for COP1-interacting
protein 4.1, GI:13160649
Length = 976
Score = 28.7 bits (61), Expect = 1.7
Identities = 20/75 (26%), Positives = 36/75 (48%)
Frame = +2
Query: 185 EKNGKCSSXEYKLEGDVVKVKNVHIIDGVKKYIEWTAKLTDDANKAAKLTVTFKFGEISR 364
EK+GK SS K + + V+ ++D +++ E L K++K + K ++
Sbjct: 665 EKSGKKSSKRSKKKDSLNIVEEAQVVDSLQQKKEAEENLEKSGKKSSKRSK--KKDSLNI 722
Query: 365 DGSVQVLATDYNNYA 409
QVL+ + NN A
Sbjct: 723 VEEAQVLSVEVNNVA 737
>At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor,
putative (AVDE1) similar to EST gb|N37612
Length = 462
Score = 28.3 bits (60), Expect = 2.3
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 122 NFNLTAYQGIWYEISKFPNESEKNGKCSSXEYKLEGDVVKVKNV 253
NFN++ + G WY I+ N + C E+ EGD V N+
Sbjct: 206 NFNISDFNGKWY-ITSGLNPTFDAFDCQLHEFHTEGDNKLVGNI 248
>At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 345
Score = 27.9 bits (59), Expect = 3.0
Identities = 14/35 (40%), Positives = 24/35 (68%), Gaps = 2/35 (5%)
Frame = +2
Query: 161 ISKFPNESEKNGKCSSXEYKLEG--DVVKVKNVHI 259
+S FP ES+ + K ++ E+KL G + V VKN+++
Sbjct: 14 VSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYL 48
>At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 527
Score = 27.9 bits (59), Expect = 3.0
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +2
Query: 227 GDVVKVKNVHIIDGVKKYIEWTAKLT 304
GD+VK +NV + K Y+ W + LT
Sbjct: 244 GDIVKARNVFDMIPHKDYVSWNSMLT 269
>At2g29070.2 68415.m03533 ubiquitin fusion degradation UFD1 family
protein similar to SP|P70362 Ubiquitin fusion
degradation protein 1 homolog (UB fusion protein 1) {Mus
musculus}; contains Pfam profile PF03152: Ubiquitin
fusion degradation protein UFD1
Length = 312
Score = 27.5 bits (58), Expect = 4.0
Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +2
Query: 224 EGDVVKVKNVHIIDGVK-KYIEWTAKLTDDANKAAKLTVTFKFGEISRDGSVQVLATDYN 400
EGDV++VKN+ ++ G K T D +N A L T + G + YN
Sbjct: 91 EGDVMQVKNISLVKGTYIKLQPHTQDFLDISNPKAILETTLRSYSCLTTG--DTIMVPYN 148
Query: 401 N 403
N
Sbjct: 149 N 149
>At2g29070.1 68415.m03534 ubiquitin fusion degradation UFD1 family
protein similar to SP|P70362 Ubiquitin fusion
degradation protein 1 homolog (UB fusion protein 1) {Mus
musculus}; contains Pfam profile PF03152: Ubiquitin
fusion degradation protein UFD1
Length = 280
Score = 27.5 bits (58), Expect = 4.0
Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +2
Query: 224 EGDVVKVKNVHIIDGVK-KYIEWTAKLTDDANKAAKLTVTFKFGEISRDGSVQVLATDYN 400
EGDV++VKN+ ++ G K T D +N A L T + G + YN
Sbjct: 59 EGDVMQVKNISLVKGTYIKLQPHTQDFLDISNPKAILETTLRSYSCLTTG--DTIMVPYN 116
Query: 401 N 403
N
Sbjct: 117 N 117
>At5g36740.1 68418.m04402 PHD finger family protein
Length = 1179
Score = 27.1 bits (57), Expect = 5.2
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 200 CSSXEYKLEGDVVKVKNVHIIDGVKKYIEWTAKLTD 307
C Y EGD + N+ ++DG + E T KLTD
Sbjct: 1020 CLKLTYVEEGDNDRESNLKLLDGSVEEKEDTKKLTD 1055
>At5g36670.1 68418.m04388 PHD finger family protein
Length = 1193
Score = 27.1 bits (57), Expect = 5.2
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 200 CSSXEYKLEGDVVKVKNVHIIDGVKKYIEWTAKLTD 307
C Y EGD + N+ ++DG + E T KLTD
Sbjct: 1034 CLKLTYVEEGDNDRESNLKLLDGSVEEKEDTKKLTD 1069
>At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative
similar to SP|P04051 DNA-directed RNA polymerase III
largest subunit (EC 2.7.7.6) {Saccharomyces cerevisiae};
contains InterPro accession IPR000722: RNA polymerase,
alpha subunit
Length = 1328
Score = 26.6 bits (56), Expect = 6.9
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = +2
Query: 278 YIEWTAKLTDDANKAAKLTVTFKFGEISRDGSVQVLATDYNNYAIA 415
Y + ++ KA F G ++DG +L DYN++A A
Sbjct: 609 YFRNSELISGQLGKATLALDIFPLGNGNKDGLYSILLRDYNSHAAA 654
>At5g37100.1 68418.m04453 replication protein-related weak
similarity to Replication Protein A 70 kDa DNA-binding
subunit (RP-A) (RF-A) (Replication factor-A protein 1)
(Single-stranded DNA-binding protein) (DMRPA1)
(GI:2498844)(SP:Q24492) [Drosophila melanogaster]
Length = 269
Score = 26.6 bits (56), Expect = 6.9
Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = +2
Query: 119 NNFNLTAYQGIWYEISKFP-NESEKNGKCSSXEYKLE 226
NNF + ++G WY I+ F + K SS Y++E
Sbjct: 61 NNFKPSLFEGKWYYIADFRLKRATSIPKYSSFNYEIE 97
>At3g05150.1 68416.m00559 sugar transporter family protein similar
to sugar-porter family proteins 1 and 2 [Arabidopsis
thaliana] GI:14585699, GI:14585701; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 470
Score = 26.6 bits (56), Expect = 6.9
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 162 SRNFPTNLRRTANALXLNTNWKVTW 236
S FP NL+ TA L NW +W
Sbjct: 394 SEIFPINLKGTAGGLVTVVNWLSSW 418
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 26.2 bits (55), Expect = 9.1
Identities = 17/66 (25%), Positives = 29/66 (43%)
Frame = +2
Query: 215 YKLEGDVVKVKNVHIIDGVKKYIEWTAKLTDDANKAAKLTVTFKFGEISRDGSVQVLATD 394
Y D + +++ IID + W + NK LT+ KF I +D ++ L+ +
Sbjct: 640 YNFTQDDLMTEDIFIIDCHSEIFVWVGQEVVPKNKLLALTIGEKF--IEKDSLLEKLSPE 697
Query: 395 YNNYAI 412
Y I
Sbjct: 698 APIYVI 703
>At1g59560.1 68414.m06698 expressed protein contains similarity to
apoptosis inhibitors
Length = 338
Score = 26.2 bits (55), Expect = 9.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 183 LRRTANALXLNTNWKVTW*RSRT 251
L+RTA L NW+ +W R+ T
Sbjct: 78 LKRTAEQQVLRRNWRFSWVRNST 100
>At1g08890.1 68414.m00989 sugar transporter family protein similar
to sugar-porter family proteins 1 and 2 [Arabidopsis
thaliana] GI:14585699, GI:14585701; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 464
Score = 26.2 bits (55), Expect = 9.1
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +3
Query: 162 SRNFPTNLRRTANALXLNTNWKVTW 236
S FP N++ TA +L +NW +W
Sbjct: 385 SEVFPVNVKITAGSLVTVSNWFFSW 409
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,794,824
Number of Sequences: 28952
Number of extensions: 136576
Number of successful extensions: 362
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 361
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 655255392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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