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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0193
         (745 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g00340.1 68417.m00045 S-locus glycoprotein family protein / c...    29   2.5  
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro...    28   5.7  
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro...    28   5.7  
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN...    28   5.7  
At5g10440.1 68418.m01210 cyclin family protein similar to cyclin...    28   7.5  
At4g31530.1 68417.m04477 expressed protein                             27   9.9  
At1g33440.1 68414.m04139 proton-dependent oligopeptide transport...    27   9.9  

>At4g00340.1 68417.m00045 S-locus glycoprotein family protein /
           curculin-like (mannose-binding) lectin family protein /
           PAN domain-containing protein contains Pfam profiles:
           PF01453 lectin (probable mannose binding), PF00954
           S-locus glycoprotein family, PF00024 PAN domain
          Length = 402

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
 Frame = -2

Query: 417 SR*MADRCSSTKTCL----CRGYYPMSRSHICYSLHLLDKSLSNLPSPSSRT 274
           SR    + S  KTCL    C G+Y   +S++C    +L +S +NL + SS T
Sbjct: 350 SRLQVSKSSCAKTCLGNSSCVGFYHKEKSNLC---KILLESPNNLKNSSSET 398


>At5g54650.2 68418.m06805 formin homology 2 domain-containing protein
            / FH2 domain-containing protein contains formin homology
            2 domain, Pfam:PF02181
          Length = 900

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 16/67 (23%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
 Frame = +2

Query: 71   LIRQYAVLLDIMSRAARSFP-QPSRSSKNNNDTTGWRSVFYL*DESVQPRAQLFPVLSVL 247
            ++R + ++LD   +  R    +P R ++    T    S       S+ PR +LFP ++  
Sbjct: 831  IVRDFLIILDKSCKEVREARGRPVRMARKQGSTASASSETPRQTPSLDPRQKLFPAITER 890

Query: 248  QIIRSST 268
            ++ +SS+
Sbjct: 891  RVDQSSS 897


>At5g54650.1 68418.m06804 formin homology 2 domain-containing protein
            / FH2 domain-containing protein contains formin homology
            2 domain, Pfam:PF02181
          Length = 900

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 16/67 (23%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
 Frame = +2

Query: 71   LIRQYAVLLDIMSRAARSFP-QPSRSSKNNNDTTGWRSVFYL*DESVQPRAQLFPVLSVL 247
            ++R + ++LD   +  R    +P R ++    T    S       S+ PR +LFP ++  
Sbjct: 831  IVRDFLIILDKSCKEVREARGRPVRMARKQGSTASASSETPRQTPSLDPRQKLFPAITER 890

Query: 248  QIIRSST 268
            ++ +SS+
Sbjct: 891  RVDQSSS 897


>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
            topoisomerase II / DNA gyrase (TOP2) identical to
            SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis
            thaliana}
          Length = 1473

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 14/30 (46%), Positives = 17/30 (56%)
 Frame = +3

Query: 654  RCYYYNYGSIPVSSAQG*CQKAAIADDDDD 743
            R   YN+GS P  S++      AIA DDDD
Sbjct: 1306 RLAQYNFGSAPADSSKTAETSKAIAVDDDD 1335


>At5g10440.1 68418.m01210 cyclin family protein similar to cyclin
           D2.1 protein [Nicotiana tabacum] GI:4160298; contains
           Pfam profile PF00134: Cyclin, N-terminal domain
          Length = 298

 Score = 27.9 bits (59), Expect = 7.5
 Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 5/69 (7%)
 Frame = +3

Query: 177 VQYFISRMNPYSPELNYFL---SYQYCRSL--GLQLASFETKEKADSITTYLTNAGYNKY 341
           V+YF+S++N Y  E +  L   S Q   S   G+    F   E A ++   ++   ++K+
Sbjct: 177 VRYFLSKINGYDQEPHSRLVTRSLQVIASTTKGIDFLEFRASEIAAAVALSVSGEHFDKF 236

Query: 342 DFWTSGNNL 368
            F +S ++L
Sbjct: 237 SFSSSFSSL 245


>At4g31530.1 68417.m04477 expressed protein
          Length = 324

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 11/35 (31%), Positives = 19/35 (54%)
 Frame = -3

Query: 647 LAYEVLDLAAVLRLPVEGRSFERDAAISHDVFRPG 543
           L + +++L  VL+    G  F RD+ +   + RPG
Sbjct: 202 LPWSIMNLFGVLKYKKMGEDFLRDSGLPFTIIRPG 236


>At1g33440.1 68414.m04139 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family
          Length = 601

 Score = 27.5 bits (58), Expect = 9.9
 Identities = 28/129 (21%), Positives = 48/129 (37%), Gaps = 7/129 (5%)
 Frame = +3

Query: 174 GVQYFISRMNPYSPELNYFLSYQYCR-SLGLQLASFETKEKADSITTYLTNAGYNKYDFW 350
           G+  F  + +  S + ++  +  YC  S G  L+S        ++    ++ G    + W
Sbjct: 477 GLVEFFYKQSSQSMQ-SFLTAMTYCSYSFGFYLSSV----LVSTVNRVTSSNGSGTKEGW 531

Query: 351 TSGNNLGTDM---FLWMSTGLPFNATFNYM---RRLPIDAPAQHADDSMDPLDVPQGSTA 512
              N+L  D    F W+   L F   FNY+   R    D  A H    ++ L+  +    
Sbjct: 532 LGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSAEVNSLEALENGEI 591

Query: 513 PQRTARHGR 539
              T    R
Sbjct: 592 KDSTTEKPR 600


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,383,445
Number of Sequences: 28952
Number of extensions: 354000
Number of successful extensions: 901
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 879
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 900
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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