Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0180
(662 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40730.1 68415.m05024 HEAT repeat-containing protein contains... 171 4e-43
At3g25800.1 68416.m03211 serine/threonine protein phosphatase 2A... 34 0.097
At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A... 33 0.22
At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A... 31 0.52
At1g48090.2 68414.m05363 C2 domain-containing protein contains P... 30 1.2
At1g48090.1 68414.m05362 C2 domain-containing protein contains P... 30 1.2
At2g02560.1 68415.m00195 TIP120 protein, putative similar to TBP... 29 3.6
At3g22200.1 68416.m02801 4-aminobutyrate aminotransferase / gamm... 28 6.4
At1g67140.1 68414.m07638 expressed protein 28 6.4
At1g30640.1 68414.m03747 protein kinase, putative contains prote... 28 6.4
>At2g40730.1 68415.m05024 HEAT repeat-containing protein contains
INTERPRO:IPR000357 HEAT repeat
Length = 798
Score = 171 bits (416), Expect = 4e-43
Identities = 87/208 (41%), Positives = 135/208 (64%), Gaps = 1/208 (0%)
Frame = +2
Query: 20 LSXANYXQDPDYQKQIVPCVVKLFASNDRTTRSRLLQQLDQFIMHLQNSTVNDQIFPQVV 199
L ++ D+ +++P +VKLFASNDR R LLQ +DQF + V++Q++P V
Sbjct: 351 LKMGSWLSTEDFSVKVLPTIVKLFASNDRAIRVSLLQHVDQFGESMSGQIVDEQVYPHVA 410
Query: 200 NGFLDTNAIIREQTVKSIVHLASKLNYNNLNVEVLRHFARLQSKDDQGGIRTNTTVCLGK 379
GF DT+A +RE T+KS++ LA KL+ L+ +L++ ++LQ D++ IRTNTT+ LG
Sbjct: 411 TGFADTSAFLRELTLKSMLVLAPKLSQRTLSGSLLKYLSKLQ-VDEEPAIRTNTTILLGN 469
Query: 380 IAAHLHPQIRQKVLVSAF-VRSTRDPFPPARQAGVLALAATQQYFLLSEVANRXLPALCP 556
IA +L+ R++VL++AF VR+ RD FPPAR AG++AL AT + +E+A R LP +
Sbjct: 470 IATYLNEGTRKRVLINAFTVRALRDTFPPARGAGIVALCATSTTYDDTEIATRILPNIVV 529
Query: 557 LTIDP*KQVRDAAFKTIRGFSR*TRESF 640
LTID VR AF+ + F + ++++
Sbjct: 530 LTIDQDSDVRSKAFQAVEQFLQILKQNY 557
>At3g25800.1 68416.m03211 serine/threonine protein phosphatase 2A
(PP2A) 65 KDa regulatory subunit A identical to protein
phosphatase 2A 65 kDa regulatory subunit (pDF1)
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 33.9 bits (74), Expect = 0.097
Identities = 40/197 (20%), Positives = 67/197 (34%)
Frame = +2
Query: 17 CLSXANYXQDPDYQKQIVPCVVKLFASNDRTTRSRLLQQLDQFIMHLQNSTVNDQIFPQV 196
C + + D + I+P +V R + QL + + ++ P
Sbjct: 226 CAALGKLLEPQDCVQHILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPEPTRTELVPAY 285
Query: 197 VNGFLDTNAIIREQTVKSIVHLASKLNYNNLNVEVLRHFARLQSKDDQGGIRTNTTVCLG 376
V D A +R + LN +L L S Q +V +G
Sbjct: 286 VRLLRDNEAEVRIAAAGKVTKFCRILNPEIAIQHILPCVKELSSDSSQHVRSALASVIMG 345
Query: 377 KIAAHLHPQIRQKVLVSAFVRSTRDPFPPARQAGVLALAATQQYFLLSEVANRXLPALCP 556
+A L + L+ F+ +D FP R + L Q + ++ LPA+
Sbjct: 346 -MAPVLGKDATIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVE 404
Query: 557 LTIDP*KQVRDAAFKTI 607
L D +VR A + I
Sbjct: 405 LAEDRHWRVRLAIIEYI 421
Score = 29.1 bits (62), Expect = 2.8
Identities = 36/184 (19%), Positives = 68/184 (36%)
Frame = +2
Query: 35 YXQDPDYQKQIVPCVVKLFASNDRTTRSRLLQQLDQFIMHLQNSTVNDQIFPQVVNGFLD 214
Y +Y ++P + L + R + ++ L + ++ S + D F +V
Sbjct: 77 YVGGVEYAHVLLPPLETLSTVEETCVREKAVESLCRVGSQMRESDLVDH-FISLVKRLAA 135
Query: 215 TNAIIREQTVKSIVHLASKLNYNNLNVEVLRHFARLQSKDDQGGIRTNTTVCLGKIAAHL 394
+ + H+A + L E+ + +L +DD +R LGK AA +
Sbjct: 136 GEWFTARVSACGVFHIAYPSAPDMLKTELRSLYTQL-CQDDMPMVRRAAATNLGKFAATV 194
Query: 395 HPQIRQKVLVSAFVRSTRDPFPPARQAGVLALAATQQYFLLSEVANRXLPALCPLTIDP* 574
+ ++S F T+D R V AA + + LP + + D
Sbjct: 195 ESAHLKTDVMSMFEDLTQDDQDSVRLLAVEGCAALGKLLEPQDCVQHILPVIVNFSQDKS 254
Query: 575 KQVR 586
+VR
Sbjct: 255 WRVR 258
>At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit A (RCN1) identical to
phosphoprotein phosphatase 2A, regulatory subunit A
GI:1262171 from [Arabidopsis thaliana]
Length = 588
Score = 32.7 bits (71), Expect = 0.22
Identities = 37/197 (18%), Positives = 68/197 (34%)
Frame = +2
Query: 17 CLSXANYXQDPDYQKQIVPCVVKLFASNDRTTRSRLLQQLDQFIMHLQNSTVNDQIFPQV 196
C + + D +I+P +V R + QL + + + P
Sbjct: 226 CAALGKLLEPQDCVARILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPDCTRTDLVPAY 285
Query: 197 VNGFLDTNAIIREQTVKSIVHLASKLNYNNLNVEVLRHFARLQSKDDQGGIRTNTTVCLG 376
V D A +R + LN L ++ + + S D +R+ +
Sbjct: 286 VRLLRDNEAEVRIAAAGKVTKFCRLLN-PELAIQHILPCVKELSSDSSQHVRSALASVIM 344
Query: 377 KIAAHLHPQIRQKVLVSAFVRSTRDPFPPARQAGVLALAATQQYFLLSEVANRXLPALCP 556
+A L + L+ F+ +D FP R + L Q + ++ LPA+
Sbjct: 345 GMAPILGKDSTIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVE 404
Query: 557 LTIDP*KQVRDAAFKTI 607
L D +VR A + +
Sbjct: 405 LAEDRHWRVRLAIIEYV 421
>At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A
(PP2A) 65 kDa regulatory subunit, putative similar to
protein phosphatase 2A 65 kDa regulatory subunit
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 31.5 bits (68), Expect = 0.52
Identities = 37/197 (18%), Positives = 66/197 (33%)
Frame = +2
Query: 17 CLSXANYXQDPDYQKQIVPCVVKLFASNDRTTRSRLLQQLDQFIMHLQNSTVNDQIFPQV 196
C + + D I+P +V R + QL + + + P
Sbjct: 226 CAALGKLLEPQDCVAHILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPEPTRTDLVPAY 285
Query: 197 VNGFLDTNAIIREQTVKSIVHLASKLNYNNLNVEVLRHFARLQSKDDQGGIRTNTTVCLG 376
D A +R + LN L ++ + + S D +R+ +
Sbjct: 286 ARLLCDNEAEVRIAAAGKVTKFCRILN-PELAIQHILPCVKELSSDSSQHVRSALASVIM 344
Query: 377 KIAAHLHPQIRQKVLVSAFVRSTRDPFPPARQAGVLALAATQQYFLLSEVANRXLPALCP 556
+A L + L+ F+ +D FP R + L Q + ++ LPA+
Sbjct: 345 GMAPVLGKDATIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVE 404
Query: 557 LTIDP*KQVRDAAFKTI 607
L D +VR A + I
Sbjct: 405 LAEDRHWRVRLAIIEYI 421
>At1g48090.2 68414.m05363 C2 domain-containing protein contains Pfam
profile: PF00168 C2 domain
Length = 3427
Score = 30.3 bits (65), Expect = 1.2
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
Frame = +2
Query: 284 NLNVEVLRHFARLQSKDDQGGIRT---NTTVCLGKIAAHLHPQIRQKVLVSAFVRSTRDP 454
N+N E+ L D G+ T +TTV K+A H P+ VL+S+ ST +P
Sbjct: 2442 NVNAEIKLRCFSLTLLDGLHGMMTPLFDTTVTNIKLATHGRPEAMNAVLISSIAASTFNP 2501
>At1g48090.1 68414.m05362 C2 domain-containing protein contains Pfam
profile: PF00168 C2 domain
Length = 4144
Score = 30.3 bits (65), Expect = 1.2
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
Frame = +2
Query: 284 NLNVEVLRHFARLQSKDDQGGIRT---NTTVCLGKIAAHLHPQIRQKVLVSAFVRSTRDP 454
N+N E+ L D G+ T +TTV K+A H P+ VL+S+ ST +P
Sbjct: 2442 NVNAEIKLRCFSLTLLDGLHGMMTPLFDTTVTNIKLATHGRPEAMNAVLISSIAASTFNP 2501
>At2g02560.1 68415.m00195 TIP120 protein, putative similar to
TBP-interacting protein TIP120 GI:1799570 from [Rattus
norvegicus]
Length = 1219
Score = 28.7 bits (61), Expect = 3.6
Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 1/118 (0%)
Frame = +2
Query: 269 KLNYNNLNVEVLRHFARLQSKDDQGGIRTNTTVCLGKIAAHLHPQIRQKVLVSAFVRSTR 448
K ++ N +VE + + ++ G+R CLGK+A + P +K++ + VR+T
Sbjct: 921 KADFQNSSVEKILALLFNHCESEEEGVRNVVAECLGKMAL-IEP---EKLVPALQVRTTS 976
Query: 449 D-PFPPARQAGVLALAATQQYFLLSEVANRXLPALCPLTIDP*KQVRDAAFKTIRGFS 619
F A + + ++ L E+ + + L D + VR AA + F+
Sbjct: 977 PAAFTRATVVTAVKYSVVERPEKLDEIIFPQISSFLMLIKDGDRHVRRAAVSALSTFA 1034
>At3g22200.1 68416.m02801 4-aminobutyrate aminotransferase /
gamma-amino-N-butyrate transaminase / GABA transaminase
/ beta-alanine--oxoglutarate aminotransferase identical
to gamma-aminobutyrate transaminase subunit precursor
[Arabidopsis thaliana] (EC 2.6.1.19) GI:14030435;
contains Pfam profile PF00202: aminotransferase, class
III; identical to cDNA gamma-aminobutyrate transaminase
subunit precursor, nuclear gene for mitochondrial
product GI:14030434
Length = 504
Score = 27.9 bits (59), Expect = 6.4
Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +2
Query: 416 VLVSAFV--RSTRDPFPPARQAGVLALAATQQYFLLSEVANRXLPALCPLTIDP 571
+L + FV +S +PFPP G A Q++ +L VA + PL I P
Sbjct: 421 ILGTEFVDNKSPNEPFPPEWGVGAFFGAECQKHGMLVRVAGDGILMSPPLIISP 474
>At1g67140.1 68414.m07638 expressed protein
Length = 2158
Score = 27.9 bits (59), Expect = 6.4
Identities = 16/63 (25%), Positives = 32/63 (50%)
Frame = +2
Query: 140 QFIMHLQNSTVNDQIFPQVVNGFLDTNAIIREQTVKSIVHLASKLNYNNLNVEVLRHFAR 319
+FIM LQ ++ D++ ++ FL+ ++ +T + L N+ V ++ H A+
Sbjct: 1799 RFIMLLQTHSITDELQKGFMSLFLEVVLVVFSKTSDGVSQ--EVLELRNVAVRLVSHLAQ 1856
Query: 320 LQS 328
L S
Sbjct: 1857 LPS 1859
>At1g30640.1 68414.m03747 protein kinase, putative contains protein
kinase domain, Pfam:PF00069
Length = 562
Score = 27.9 bits (59), Expect = 6.4
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -3
Query: 159 CRCIMNWSSCCRSLDLVVLSLEANSL 82
CR I+NW +C + D LS+E L
Sbjct: 372 CRKIVNWKTCLKFPDEAKLSIEVKDL 397
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,486,136
Number of Sequences: 28952
Number of extensions: 264262
Number of successful extensions: 752
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 749
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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