Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0164
(498 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g44070.1 68418.m05392 phytochelatin synthase 1 (PCS1) identic... 30 0.75
At5g61350.1 68418.m07698 protein kinase family protein contains ... 29 2.3
At3g18890.1 68416.m02399 expressed protein similar to UV-B and o... 29 2.3
At5g61190.1 68418.m07676 zinc finger protein-related contains Pf... 28 3.0
At5g39770.1 68418.m04817 repair endonuclease family protein cont... 28 3.0
At5g07060.1 68418.m00799 zinc finger (CCCH-type) family protein ... 28 3.0
At3g20290.1 68416.m02571 calcium-binding EF hand family protein ... 28 3.0
At2g04865.1 68415.m00502 expressed protein ; expression supporte... 28 4.0
At1g79670.2 68414.m09292 wall-associated kinase, putative simila... 28 4.0
At1g79670.1 68414.m09291 wall-associated kinase, putative simila... 28 4.0
At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S), ... 27 5.3
At1g11620.1 68414.m01334 F-box family protein contains F-box dom... 27 5.3
At5g43530.1 68418.m05322 SNF2 domain-containing protein / helica... 27 9.3
At3g56050.1 68416.m06227 protein kinase family protein contains ... 27 9.3
At1g60630.1 68414.m06825 leucine-rich repeat family protein simi... 27 9.3
At1g34670.1 68414.m04311 myb family transcription factor similar... 27 9.3
>At5g44070.1 68418.m05392 phytochelatin synthase 1 (PCS1) identical
to phytochelatin synthase [Arabidopsis thaliana]
gi|18254401|gb|AAL66747; identical to cDNA
phytochelatin synthase, GI:18254400
Length = 485
Score = 30.3 bits (65), Expect = 0.75
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = +2
Query: 308 KPCCRISATCRSPPSRATSCGTTCAGC*RRPSPASGT 418
K CC+ + PS+ C TC C + P + GT
Sbjct: 340 KACCQGAEILSGSPSKEFCCRETCVKCIKGPDDSEGT 376
>At5g61350.1 68418.m07698 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 842
Score = 28.7 bits (61), Expect = 2.3
Identities = 20/55 (36%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +1
Query: 25 SKS-NDLDLMNNIVNLLTSIHTLFYMSLTEEIGDKQKPDHIYEGRDMTSLVILLQ 186
SKS NDL + N +N L++I L SLT +G D + +T+ IL+Q
Sbjct: 318 SKSLNDL-IFNVFINKLSAISALDLSSLTSALGTAYYADFVLNASTITNGSILVQ 371
>At3g18890.1 68416.m02399 expressed protein similar to UV-B and
ozone similarly regulated protein 1 UOS1 [Pisum sativum]
GI:20339364
Length = 641
Score = 28.7 bits (61), Expect = 2.3
Identities = 13/38 (34%), Positives = 24/38 (63%)
Frame = +1
Query: 379 CRLLTTPVTSVRDLVAEFLFVLCKEKVSRMVKYTGFGN 492
C L P+T++ L + F+L + K +R+V+++GF N
Sbjct: 5 CFLRGQPLTTIPSLPSRKGFLLQRWKTNRIVRFSGFKN 42
>At5g61190.1 68418.m07676 zinc finger protein-related contains Pfam
profile PF04396: Protein of unknown function DUF537,
weak hit to PF00096: Zinc finger C2H2 type
Length = 977
Score = 28.3 bits (60), Expect = 3.0
Identities = 26/95 (27%), Positives = 49/95 (51%), Gaps = 4/95 (4%)
Frame = +1
Query: 217 ERQNLYEKLSPILTVLNKCARVRVQ-RKFLRKAVLPHLRDVSKPPEQGDELRNHLCRLLT 393
+ + + EK P TV A +++Q +K K V L D K PE+GDE++
Sbjct: 414 QEKGVGEKEQPSETV----AELQLQSQKAQEKQVPMVLVDSKKLPEKGDEVKGQ-----P 464
Query: 394 TPVTSVRDLVAEFLFVLCKEKV---SRMVKYTGFG 489
+T++R+ A+++ +C ++ S+ ++ GFG
Sbjct: 465 KEMTALRNASAKYICRMCNDQALIDSKKLQEKGFG 499
>At5g39770.1 68418.m04817 repair endonuclease family protein contains
Pfam PF02732 : ERCC4 domain; similar to MUS81
endonuclease (GI:16755674) [Mus musculus]; similar to
repair endonuclease (TIGR:At5g41150) [Arabidopsis
thaliana]
Length = 1242
Score = 28.3 bits (60), Expect = 3.0
Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Frame = -2
Query: 452 SLQSTNKNSATKSLTLVTGVVS---SRHRWFRSSSPCSGGFDTSRRCGSTALRKNLRWTR 282
S+ + + A + L TG V SR + FRSSSPCS + + S++L +
Sbjct: 918 SIPAGPSSRAQEFLATDTGQVDLEGSRAKKFRSSSPCSSSISHALKACSSSLASDGTEGV 977
Query: 281 TRAHLLRTVNIGDSF 237
T L + G++F
Sbjct: 978 TTIPRLPPLRFGEAF 992
>At5g07060.1 68418.m00799 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 363
Score = 28.3 bits (60), Expect = 3.0
Identities = 14/50 (28%), Positives = 27/50 (54%)
Frame = +1
Query: 76 SIHTLFYMSLTEEIGDKQKPDHIYEGRDMTSLVILLQFLKHQLTASDERQ 225
SI TL+ L I ++ DH Y +M S+ ++ + K+ + S+++Q
Sbjct: 223 SIKTLYVGGLNSRIFEQDIHDHFYAYGEMESIRVMAEDGKYDQSGSNQQQ 272
>At3g20290.1 68416.m02571 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4 and {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 545
Score = 28.3 bits (60), Expect = 3.0
Identities = 23/69 (33%), Positives = 33/69 (47%), Gaps = 10/69 (14%)
Frame = +1
Query: 118 GDKQKPDHIYEGRDMTSL------VILLQFLKHQLTASDERQNLYEKL----SPILTVLN 267
G+KQ+ Y+ +TS +ILL F H+L SDE + + L I VLN
Sbjct: 299 GEKQRTQRAYDFTGVTSWFASKCDLILLLFDPHKLDVSDEFKRVISSLRGHDDKIRVVLN 358
Query: 268 KCARVRVQR 294
K +V Q+
Sbjct: 359 KADQVDTQQ 367
>At2g04865.1 68415.m00502 expressed protein ; expression supported
by MPSS
Length = 667
Score = 27.9 bits (59), Expect = 4.0
Identities = 16/62 (25%), Positives = 24/62 (38%), Gaps = 1/62 (1%)
Frame = -2
Query: 470 TMRLTFSLQSTN-KNSATKSLTLVTGVVSSRHRWFRSSSPCSGGFDTSRRCGSTALRKNL 294
TM ++F + + K L + + RW R S GG D S + S +
Sbjct: 330 TMLISFDKAERHLPDRCRKQFDLFQDIPADVQRWVRKSRGVDGGVDLSNKMESELSEWEM 389
Query: 293 RW 288
RW
Sbjct: 390 RW 391
>At1g79670.2 68414.m09292 wall-associated kinase, putative similar
to wall-associated kinase 4 GI:3355308 from [Arabidopsis
thaliana]; isoform contains non-consensus AT-acceptor
splice site.
Length = 714
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 332 TCRSPPSRATSCGTTCAG 385
T +SPPS +TSC C G
Sbjct: 26 TAQSPPSSSTSCNRICGG 43
>At1g79670.1 68414.m09291 wall-associated kinase, putative similar
to wall-associated kinase 4 GI:3355308 from [Arabidopsis
thaliana]; isoform contains non-consensus AT-acceptor
splice site.
Length = 751
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 332 TCRSPPSRATSCGTTCAG 385
T +SPPS +TSC C G
Sbjct: 26 TAQSPPSSSTSCNRICGG 43
>At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S),
Arabidopsis thaliana, PIR:B71412
Length = 303
Score = 27.5 bits (58), Expect = 5.3
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -2
Query: 389 SSRHRWFRSSSPCSGGFDTSRRCGSTALRKNLR 291
+S R SSSP G F +S R A ++NLR
Sbjct: 15 ASSSRSLASSSPSPGSFSSSMRLWRPAAQRNLR 47
>At1g11620.1 68414.m01334 F-box family protein contains F-box domain
Pfam:PF00646
Length = 363
Score = 27.5 bits (58), Expect = 5.3
Identities = 18/54 (33%), Positives = 25/54 (46%)
Frame = -2
Query: 491 LPNPVYFTMRLTFSLQSTNKNSATKSLTLVTGVVSSRHRWFRSSSPCSGGFDTS 330
LP P+ + + S TKS+ LV + S+ RW + CS GFD S
Sbjct: 91 LPFPIALSPAINISHCDGLLLYVTKSMLLVANPLLSQKRWIK----CSEGFDHS 140
>At5g43530.1 68418.m05322 SNF2 domain-containing protein / helicase
domain-containing protein / RING finger
domain-containing protein similar to SP|P36607 DNA
repair protein rad8 {Schizosaccharomyces pombe};
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 1277
Score = 26.6 bits (56), Expect = 9.3
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Frame = +2
Query: 74 QASTHCFIC----P*QKKLETNKSLTIFMKVEI*LHWSYY 181
+ S+HC C P Q KLE SL F+ VE +W+++
Sbjct: 840 ELSSHCRWCLTGTPLQNKLEDLYSLLCFLHVEPWCNWAWW 879
>At3g56050.1 68416.m06227 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 499
Score = 26.6 bits (56), Expect = 9.3
Identities = 23/109 (21%), Positives = 48/109 (44%), Gaps = 1/109 (0%)
Frame = +1
Query: 16 QTSSKSNDLDLMNNIVNLLTSIHTLFYMSLTEEIGDKQKPDHIYEGRDM-TSLVILLQFL 192
+T S +N L + NI + + L E+ + P+ + +G + T L + L+
Sbjct: 367 ETESSTNAL-IDTNISETTQEDNVHSFGLLLFELMTGKLPESVQKGDSIDTGLAVFLRGK 425
Query: 193 KHQLTASDERQNLYEKLSPILTVLNKCARVRVQRKFLRKAVLPHLRDVS 339
+ ++ EK+ I V+ C R +++ + K V LR+++
Sbjct: 426 TLREMVDPTIESFDEKIENIGEVIKSCIRADAKQRPIMKEVTGRLREIT 474
>At1g60630.1 68414.m06825 leucine-rich repeat family protein similar
to receptor kinase GI:498278 from [Petunia
integrifolia]; contains protein kinase domain,
Pfam:PF00069; contains leucine-rich repeats,
Pfam:PF00560
Length = 652
Score = 26.6 bits (56), Expect = 9.3
Identities = 17/65 (26%), Positives = 33/65 (50%)
Frame = +1
Query: 139 HIYEGRDMTSLVILLQFLKHQLTASDERQNLYEKLSPILTVLNKCARVRVQRKFLRKAVL 318
H Y G D+++ V ++ + + S+E EKL +LT+ C V+ + + + VL
Sbjct: 556 HKY-GSDISTWVRAVR--EEETEVSEELNASEEKLQALLTIATACVAVKPENRPAMREVL 612
Query: 319 PHLRD 333
++D
Sbjct: 613 KMVKD 617
>At1g34670.1 68414.m04311 myb family transcription factor similar to
myb-related protein mixta GI:485867 from [Antirrhinum
majus]
Length = 365
Score = 26.6 bits (56), Expect = 9.3
Identities = 21/71 (29%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
Frame = +1
Query: 199 QLTASDERQNLYEKLSPILTVLNKCARVRVQRKFLRKAVLPHLRDV-SKPPEQGDELRNH 375
+L A+D+ Q+L+ P+ ++++ + Q+K+ HL + S+ P QGD L +H
Sbjct: 225 ELEANDQNQDLF----PLGFIIDQPTQPLQQQKY-------HLNNSPSELPSQGDPLLDH 273
Query: 376 LCRLLTTPVTS 408
+ L TP+ S
Sbjct: 274 VPFSLQTPLNS 284
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,338,476
Number of Sequences: 28952
Number of extensions: 191055
Number of successful extensions: 489
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 475
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 489
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -