BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0156
(718 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 42 7e-05
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 30 0.38
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 27 2.0
SPAC7D4.11c |sec39||secretory pathway protein Sec39 |Schizosacch... 25 8.2
SPAC23G3.03 |sib2||ornithine N5 monooxygenase |Schizosaccharomyc... 25 8.2
>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
Vps33|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 42.3 bits (95), Expect = 7e-05
Identities = 28/126 (22%), Positives = 55/126 (43%)
Frame = +1
Query: 166 KKDLIIDPSLIKALERICGVTWLRQHGVDKIYKMDPQLGPTANPNRVYFIPACIIKYKCV 345
KK L+++ L L +I L++HG+ ++Y + + +Y K V
Sbjct: 23 KKSLLLERDLSGILGQIVTTNTLQEHGIPQVYWFNENIPNDIEKKTIYLCRPTYENAKLV 82
Query: 346 LDQISSLISQNPSIADVNGFHIIIIPKVLNSFDSILESKGLYGVVKLHAFAWELMVLDDQ 525
+ I +I++P F+++L+ +G++G + + + + LD
Sbjct: 83 ATHVRQFQRDMLRIEST----VIVLPTSNILFETVLQEEGVFGELLVTEWPLHAVPLDKD 138
Query: 526 LLSLEL 543
LLSLEL
Sbjct: 139 LLSLEL 144
>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 639
Score = 29.9 bits (64), Expect = 0.38
Identities = 25/130 (19%), Positives = 56/130 (43%), Gaps = 1/130 (0%)
Frame = +1
Query: 169 KDLIIDPSLIKALERICGVTWLRQHGVDKIYKMDPQLGPTANPNRVYFIPACIIKYKCVL 348
K LI D + + + + ++ LR+HGV + P A+ +YF+ + ++
Sbjct: 47 KVLIFDKAGSETISSVLRISDLRKHGVTVHMNITSFRQPIADVPAIYFVQPTQENIELII 106
Query: 349 DQISSLISQNPSIADVNGFHIIIIPKVLNSFDSILESKGLYGVV-KLHAFAWELMVLDDQ 525
+ +S + ++ + F I +L F + ++ +++ +VL+
Sbjct: 107 EDLSKGLYESAYVC----FSSTISRALLEQFAELASKTNTSHMIHQVYDQYLNYVVLESD 162
Query: 526 LLSLELPFLF 555
SL+LP +F
Sbjct: 163 FFSLQLPKIF 172
>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 27.5 bits (58), Expect = 2.0
Identities = 19/76 (25%), Positives = 36/76 (47%), Gaps = 2/76 (2%)
Frame = -3
Query: 314 IKYTLFGFAVGPSWGSILYILSTPCCLSHVTP--HILSKALIKDGSIIKSFFSPH*LNIF 141
+K+ + ++ + ILY + C + P H+L+ L+KDG++ F P L
Sbjct: 697 LKFFVRERSITNKYEDILYKILEACFMQFRIPIQHVLN-ILVKDGTLNFCFLKPLLLKWM 755
Query: 140 CNLFCEISDNDDNLRL 93
+ I NDD +++
Sbjct: 756 NDYETRIHQNDDEIQV 771
>SPAC7D4.11c |sec39||secretory pathway protein Sec39
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 769
Score = 25.4 bits (53), Expect = 8.2
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -3
Query: 515 RTINSHANACNLTTPYKPLL 456
R +NS NAC+L P+K +L
Sbjct: 497 RRLNSLVNACSLIQPFKLIL 516
>SPAC23G3.03 |sib2||ornithine N5 monooxygenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 25.4 bits (53), Expect = 8.2
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +2
Query: 467 YMVWLNYMHSHG 502
Y +LNY+HSHG
Sbjct: 75 YFTFLNYLHSHG 86
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,157,515
Number of Sequences: 5004
Number of extensions: 69266
Number of successful extensions: 164
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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