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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0156
         (718 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g54860.1 68416.m06078 vacuolar protein sorting protein, putat...    48   5e-06
At3g51270.1 68416.m05612 RIO1 family protein contains Pfam profi...    31   0.58 
At2g17980.1 68415.m02090 sec1 family protein similar to SWISS-PR...    31   0.76 
At1g67970.1 68414.m07764 heat shock factor protein, putative (HS...    29   4.1  
At5g10770.1 68418.m01252 chloroplast nucleoid DNA-binding protei...    28   7.1  
At3g44230.1 68416.m04745 hypothetical protein                          28   7.1  
At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /...    27   9.4  
At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /...    27   9.4  

>At3g54860.1 68416.m06078 vacuolar protein sorting protein, putative
           similar to Swiss-Prot:Q63615 vacuolar protein sorting
           33A (r-vps33a) [Rattus norvegicus]; contains Pfam
           domain, PF00995: Sec1 family
          Length = 592

 Score = 48.4 bits (110), Expect = 5e-06
 Identities = 42/175 (24%), Positives = 83/175 (47%)
 Frame = +1

Query: 169 KDLIIDPSLIKALERICGVTWLRQHGVDKIYKMDPQLGPTANPNRVYFIPACIIKYKCVL 348
           K L+IDP L  ++  I   + L++ G++ +  +  +   T     VY + + +   K + 
Sbjct: 36  KCLVIDPKLSGSVSLIIPTSKLKELGLE-LRHLTAEPVQTECTKVVYLVRSQLSFMKFIA 94

Query: 349 DQISSLISQNPSIADVNGFHIIIIPKVLNSFDSILESKGLYGVVKLHAFAWELMVLDDQL 528
             I + I++    A    +++  +P+   + + ILE + ++ +V +  F   ++ LD+ +
Sbjct: 95  SHIQNDIAK----AIQRDYYVYFVPRRSVACEKILEQEKVHNLVTVKEFPLYMVPLDEDV 150

Query: 529 LSLELPFLFKQLFVDQNQSLLSGVAMSLWTLFHVTGRPKVMCSFGKLSAGVLDML 693
           +S EL    K   VD + S L  +A ++  L    G    M + GK S  V D+L
Sbjct: 151 ISFELELSEKDCLVDGDVSSLWHIAKAIHELEFSFGVISKMRAKGKASVRVADIL 205


>At3g51270.1 68416.m05612 RIO1 family protein contains Pfam profile
           PF01163: RIO1 family
          Length = 472

 Score = 31.5 bits (68), Expect = 0.58
 Identities = 15/49 (30%), Positives = 30/49 (61%)
 Frame = -1

Query: 634 LSHEIMSIKTLLHQTVRTGFGQQITV*IKMVTLNSITDHRGPLIPMRMH 488
           L ++ ++IKTL+++ + TG G+QI V  K   +  +    G ++ M++H
Sbjct: 87  LGYDFLAIKTLVNRGIFTGVGRQIGV-GKESDIFEVAQEDGTILAMKLH 134


>At2g17980.1 68415.m02090 sec1 family protein similar to
           SWISS-PROT:P22213 SLY1 protein [Saccharomyces
           cerevisiae]; contains Pfam domain, PF00995: Sec1 family
          Length = 627

 Score = 31.1 bits (67), Expect = 0.76
 Identities = 34/133 (25%), Positives = 52/133 (39%), Gaps = 1/133 (0%)
 Frame = +1

Query: 169 KDLIIDPSLIKALERICGVTWLRQHGVDKIYKMDPQLGPTANPNRVYFIPACIIKYKCVL 348
           K LI D      L  +  V  LR+HGV   + +D    P  +   VYF+       + ++
Sbjct: 35  KILIYDRFCQNILSPLTHVKDLRKHGVTLFFLIDKDRQPVHDVPAVYFVQPTESNLQRII 94

Query: 349 DQISSLISQNPSIADVNGFHIIIIPKVLNSFDS-ILESKGLYGVVKLHAFAWELMVLDDQ 525
              S  +     +     F   I  K L    S  L+S  +  V K+H    E + L+D 
Sbjct: 95  ADASRSLYDTFHL----NFSSSIPRKFLEELASGTLKSGSVEKVSKVHDQYLEFVTLEDN 150

Query: 526 LLSLELPFLFKQL 564
           L SL     + Q+
Sbjct: 151 LFSLAQQSTYVQM 163


>At1g67970.1 68414.m07764 heat shock factor protein, putative (HSF5)
           / heat shock transcription factor, putative (HSTF5)
           identical to heat shock transcription factor 5 (HSF5)
           SP:Q9S7U5 from [Arabidopsis thaliana]; contains Pfam
           profile: PF00447 HSF-type DNA-binding domain
          Length = 374

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 9/84 (10%)
 Frame = +1

Query: 280 GPTANPNRVYFIPACIIKYKCVLDQIS-SLISQNPS------IADVNGFHIIIIPKVL-- 432
           G +++ +   F+  C   Y  V D  + S+IS +PS      I D   F + ++PK    
Sbjct: 10  GSSSSSSVAPFLRKC---YDMVDDSTTDSIISWSPSADNSFVILDTTVFSVQLLPKYFKH 66

Query: 433 NSFDSILESKGLYGVVKLHAFAWE 504
           ++F S +    +YG  K+ A  WE
Sbjct: 67  SNFSSFIRQLNIYGFRKVDADRWE 90


>At5g10770.1 68418.m01252 chloroplast nucleoid DNA-binding protein,
           putative similar to CND41, chloroplast nucleoid DNA
           binding protein [Nicotiana tabacum] GI:2541876; contains
           Pfam profile PF00026: Eukaryotic aspartyl protease
          Length = 474

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 4/71 (5%)
 Frame = +1

Query: 286 TANPNRVYFIPACIIKYKCVLDQISSLISQNPSIADVNGFHIIIIPKVLNSFD--SILE- 456
           T  P + Y       K K      +S +S   +  D++GF  + IPKV  SF   +++E 
Sbjct: 358 TRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVEL 417

Query: 457 -SKGLYGVVKL 486
            SKG++ V K+
Sbjct: 418 GSKGIFYVFKI 428


>At3g44230.1 68416.m04745 hypothetical protein 
          Length = 204

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 13/44 (29%), Positives = 24/44 (54%)
 Frame = +1

Query: 526 LLSLELPFLFKQLFVDQNQSLLSGVAMSLWTLFHVTGRPKVMCS 657
           L S++  F  K  F  Q+ S+ +  + S+W ++ +  +P  MCS
Sbjct: 41  LFSIDNLFSCKANFSIQSISVSASSSESVWIVYFIVKKPSFMCS 84


>At3g57050.2 68416.m06351 cystathionine beta-lyase, chloroplast /
           beta-cystathionase / cysteine lyase (CBL) identical to
           SP|P53780 Cystathionine beta-lyase, chloroplast
           precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
           (Cysteine lyase) {Arabidopsis thaliana}
          Length = 449

 Score = 27.5 bits (58), Expect = 9.4
 Identities = 9/24 (37%), Positives = 17/24 (70%)
 Frame = -3

Query: 293 FAVGPSWGSILYILSTPCCLSHVT 222
           F++  S+GS+  ++S PC +SH +
Sbjct: 385 FSIAVSFGSVKSLISMPCFMSHAS 408


>At3g57050.1 68416.m06350 cystathionine beta-lyase, chloroplast /
           beta-cystathionase / cysteine lyase (CBL) identical to
           SP|P53780 Cystathionine beta-lyase, chloroplast
           precursor (EC 4.4.1.8) (CBL) (Beta-cystathionase)
           (Cysteine lyase) {Arabidopsis thaliana}
          Length = 464

 Score = 27.5 bits (58), Expect = 9.4
 Identities = 9/24 (37%), Positives = 17/24 (70%)
 Frame = -3

Query: 293 FAVGPSWGSILYILSTPCCLSHVT 222
           F++  S+GS+  ++S PC +SH +
Sbjct: 400 FSIAVSFGSVKSLISMPCFMSHAS 423


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,025,246
Number of Sequences: 28952
Number of extensions: 337472
Number of successful extensions: 800
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 769
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 799
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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