Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0149
(772 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05900.1 68416.m00664 neurofilament protein-related similar t... 34 0.090
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 33 0.16
At5g44270.1 68418.m05418 hypothetical protein 33 0.21
At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 33 0.28
At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide famil... 32 0.36
At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide famil... 32 0.36
At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 32 0.48
At3g49990.1 68416.m05466 expressed protein 31 0.64
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 31 0.64
At4g31880.1 68417.m04531 expressed protein 31 1.1
At5g63550.1 68418.m07976 expressed protein 30 1.5
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 30 1.9
At1g34590.1 68414.m04299 hypothetical protein 30 1.9
At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family... 29 3.4
At3g20280.2 68416.m02570 PHD finger family protein contains Pfam... 29 3.4
At3g20280.1 68416.m02569 PHD finger family protein contains Pfam... 29 3.4
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 4.5
At1g12080.2 68414.m01397 expressed protein 29 4.5
At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containi... 28 5.9
At2g22450.1 68415.m02662 riboflavin biosynthesis protein, putati... 28 7.9
At2g18050.1 68415.m02098 histone H1-3 (HIS1-3) similar to histon... 28 7.9
At2g02170.1 68415.m00153 remorin family protein contains Pfam do... 28 7.9
>At3g05900.1 68416.m00664 neurofilament protein-related similar to
NF-180 (GI:632549) [Petromyzon marinus] similar to
Neurofilament triplet H protein (200 kDa neurofilament
protein) (Neurofilament heavy polypeptide) (NF-H)
(Swiss-Prot:P12036) [Homo sapiens]
Length = 673
Score = 34.3 bits (75), Expect = 0.090
Identities = 27/108 (25%), Positives = 50/108 (46%), Gaps = 9/108 (8%)
Frame = +2
Query: 401 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSA-------TDAEGSADSAAII 559
+ E + +K EE A E +K E++ + ++ EEK S T GSA + I
Sbjct: 260 ELETEVEVVKAEETAEATEQAKVELEGKLEDVIVEEKDSEINSKDEKTSESGSALCSEEI 319
Query: 560 PNMVKKIDLAPNVXSDAAAVPEIKTPEAA--DAPKLADNPVDEDKPAD 697
+ +++ + P ++ A I E A + + D P +E+KP++
Sbjct: 320 LSTIQESNTDPIKETEGDASYPIDVIEKAITEEKHVVDEPANEEKPSE 367
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 33.5 bits (73), Expect = 0.16
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +2
Query: 398 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 550
P A AK+ +K ++ ++ +DS +E + +K KP+A D+ S D +
Sbjct: 141 PAAAAKNGSVKAKKESSSEDDSSSEDEPAKKPAAKIAKPAAKDSSSSDDDS 191
>At5g44270.1 68418.m05418 hypothetical protein
Length = 309
Score = 33.1 bits (72), Expect = 0.21
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +3
Query: 294 KRTI*PQKTPISRNRKPFLK*NPKKKSRKQNHPRFPMLKQ 413
+RTI PQ P+S PFL K++ K N P+ ++++
Sbjct: 256 RRTIVPQTRPVSNLNNPFLPHKSNKETTKPNSPKLRVIRR 295
>At4g29060.1 68417.m04157 elongation factor Ts family protein
similar to SP|P35019 Elongation factor Ts (EF-Ts)
{Galdieria sulphuraria}; contains Pfam profiles PF00627:
UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
S1 RNA binding domain
Length = 953
Score = 32.7 bits (71), Expect = 0.28
Identities = 27/99 (27%), Positives = 46/99 (46%), Gaps = 6/99 (6%)
Frame = +2
Query: 419 ADIKVEE-PAAQ-PEDSKTEVQATVAEISKEEKPSATDAEGSADSAAI----IPNMVKKI 580
A++ EE P+++ P+ + EV AT AE EK T+ +A A IP +
Sbjct: 392 AEVTSEEVPSSETPKVVEEEVIATKAEDDSPEKEEQTETLAAAAEAEEVVPPIPETKSEE 451
Query: 581 DLAPNVXSDAAAVPEIKTPEAADAPKLADNPVDEDKPAD 697
++ N +A E+ +PEA + ++ V + P D
Sbjct: 452 EIVENSIPPNSATDEVSSPEALASEEVEKEQVVAETPVD 490
Score = 31.1 bits (67), Expect = 0.84
Identities = 35/126 (27%), Positives = 56/126 (44%), Gaps = 25/126 (19%)
Frame = +2
Query: 398 PDAEAKSADIKVEEPAAQPED--------SKT------EVQATVAEISKEEKPSATDA-E 532
P+AEA +VEE P + S+T EV AT AE EK T+
Sbjct: 373 PEAEASVTSAEVEESVCVPAEVTSEEVPSSETPKVVEEEVIATKAEDDSPEKEEQTETLA 432
Query: 533 GSADSAAIIPNM--------VKKIDLAPNVXSDAAAVPEIKTPEAADAPK-LADNPVDED 685
+A++ ++P + + + + PN +D + PE E + + +A+ PVDE
Sbjct: 433 AAAEAEEVVPPIPETKSEEEIVENSIPPNSATDEVSSPEALASEEVEKEQVVAETPVDEV 492
Query: 686 K-PADI 700
K PA +
Sbjct: 493 KTPAPV 498
>At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 32.3 bits (70), Expect = 0.36
Identities = 26/86 (30%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = +2
Query: 422 DIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVKKIDLAPNVX 601
++K EEPA E +KTE T E KEE T +G A++ +++
Sbjct: 143 EVKAEEPAKTEEPAKTE--GTSGE--KEEIVEET-KKGETPETAVVEEKKPEVEEKKEEA 197
Query: 602 SDAAAVPE--IKTPEAADAPKLADNP 673
+ A AV E +K PE +A+ P
Sbjct: 198 TPAPAVVETPVKEPETTTTAPVAEPP 223
>At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 32.3 bits (70), Expect = 0.36
Identities = 26/86 (30%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = +2
Query: 422 DIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVKKIDLAPNVX 601
++K EEPA E +KTE T E KEE T +G A++ +++
Sbjct: 143 EVKAEEPAKTEEPAKTE--GTSGE--KEEIVEET-KKGETPETAVVEEKKPEVEEKKEEA 197
Query: 602 SDAAAVPE--IKTPEAADAPKLADNP 673
+ A AV E +K PE +A+ P
Sbjct: 198 TPAPAVVETPVKEPETTTTAPVAEPP 223
>At5g26200.1 68418.m03118 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 342
Score = 31.9 bits (69), Expect = 0.48
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = -2
Query: 204 LTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 100
+T+SS+ AT R+G + TTS + A G A LT+A A
Sbjct: 112 ITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146
>At3g49990.1 68416.m05466 expressed protein
Length = 502
Score = 31.5 bits (68), Expect = 0.64
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +2
Query: 395 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAE 532
+P A+ D+K E P A+P KT Q + E K+E+ +A AE
Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEE--KKERKNAVKAE 465
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 31.5 bits (68), Expect = 0.64
Identities = 29/99 (29%), Positives = 43/99 (43%)
Frame = +2
Query: 398 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVKK 577
P E K + K EE + E+ KTE ++ AE +EEK SA A I+
Sbjct: 123 PVKEEKVEEKKTEETEEKKEEVKTEEKSLEAETKEEEK-SAAPATVETKKEEILAAPAPI 181
Query: 578 IDLAPNVXSDAAAVPEIKTPEAADAPKLADNPVDEDKPA 694
+ + A P ++T A AP +A+ +E PA
Sbjct: 182 VAETKKEETPVAPAP-VETKPA--APVVAETKKEEILPA 217
>At4g31880.1 68417.m04531 expressed protein
Length = 873
Score = 30.7 bits (66), Expect = 1.1
Identities = 25/99 (25%), Positives = 40/99 (40%), Gaps = 6/99 (6%)
Frame = +2
Query: 416 SADIKVEEPAAQPEDSKTEVQATVAE---ISKEEKPSATDAEGSADSAAIIPNMVKKIDL 586
SA I EE + +P S+ +V + S + KP+ ++ S + KK+
Sbjct: 468 SASIATEEVSEEPNTSEPQVTKKSGKKVASSSKTKPTVPPSKKSTSETKVAKQSEKKVVG 527
Query: 587 APNVXSDAAAVPEIKTP---EAADAPKLADNPVDEDKPA 694
+ N E K P +A D L + D +KPA
Sbjct: 528 SDNAQESTKPKEEKKKPGRGKAIDEESLHTSSGDNEKPA 566
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 30.3 bits (65), Expect = 1.5
Identities = 17/64 (26%), Positives = 33/64 (51%)
Frame = +2
Query: 401 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVKKI 580
D + + +++VE+P+ + SK V+ + K+++PS A+GSA S + K
Sbjct: 323 DTDDEKDEVEVEKPSKKKSSSKKTVEESSGSKGKDKQPS---AKGSARSGEKSSKQIAKS 379
Query: 581 DLAP 592
+P
Sbjct: 380 TSSP 383
>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
SEC14-like protein 2 (Alpha-tocopherol associated
protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
taurus}; similar to GI:807956 from [Saccharomyces
cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 573
Score = 29.9 bits (64), Expect = 1.9
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 3/100 (3%)
Frame = +2
Query: 407 EAKSADIKVEE--PAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVKKI 580
E + ++KVEE PA + + +A E EEKP AE + + A+
Sbjct: 145 EETTTEVKVEEEKPAVPAAEEEKSSEAAPVETKSEEKPE-EKAEVTTEKASSAEE----- 198
Query: 581 DLAPNVXSDAAAVPEIKTPEAADAPKLADN-PVDEDKPAD 697
D V + ++ + PE+A AP + + V E +P +
Sbjct: 199 DGTKTVEAIEESIVSVSPPESAVAPVVVETVAVAEAEPVE 238
>At1g34590.1 68414.m04299 hypothetical protein
Length = 820
Score = 29.9 bits (64), Expect = 1.9
Identities = 27/92 (29%), Positives = 39/92 (42%), Gaps = 1/92 (1%)
Frame = +2
Query: 395 IPDAEAKSADIKVEEPAAQPEDSKT-EVQATVAEISKEEKPSATDAEGSADSAAIIPNMV 571
IPDA A D + P A PE S+ V A S DA ++ +IP+
Sbjct: 436 IPDAAAP-VDAGLISPRAAPEASRDGNVIPDAAAPVDAGLISLRDAPEASRDGNVIPDAA 494
Query: 572 KKIDLAPNVXSDAAAVPEIKTPEAADAPKLAD 667
+D AP+ +A P + EA A ++D
Sbjct: 495 APVDAAPSEAQEAE--PSVAASEAVVALPVSD 524
>At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 370
Score = 29.1 bits (62), Expect = 3.4
Identities = 27/88 (30%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
Frame = +2
Query: 413 KSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVK-KIDLA 589
K +I+ E PE K E+ TV E+ K E P + IP + K ++
Sbjct: 279 KMPEIQKPELPKVPEVPKPELP-TVPEVPKSEAPKFPEIP--KPELPKIPEVPKPELPKV 335
Query: 590 PNVXSDAAAVPEIKTPEAADAPKLADNP 673
P + A VPEI PE P+L P
Sbjct: 336 PEITKPA--VPEIPKPELPTMPQLPKLP 361
Score = 28.3 bits (60), Expect = 5.9
Identities = 27/89 (30%), Positives = 38/89 (42%), Gaps = 2/89 (2%)
Frame = +2
Query: 413 KSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVK-KIDLA 589
K +I E PE K ++ E SK E P + E A +P + K ++
Sbjct: 146 KMPEIPKPELPKFPEIPKPDLPK-FPENSKPEVPKLMETE--KPEAPKVPEIPKPELPKL 202
Query: 590 PNVXS-DAAAVPEIKTPEAADAPKLADNP 673
P V +A VPEI+ PE P+L P
Sbjct: 203 PEVPKLEAPKVPEIQKPELPKMPELPKMP 231
>At3g20280.2 68416.m02570 PHD finger family protein contains Pfam
profile: PF00628 PHD-finger
Length = 482
Score = 29.1 bits (62), Expect = 3.4
Identities = 19/73 (26%), Positives = 33/73 (45%), Gaps = 3/73 (4%)
Frame = +2
Query: 428 KVEEPAAQPEDSKTE-VQATVAEISKEEKPSATDAEG--SADSAAIIPNMVKKIDLAPNV 598
K E + P+ + + V E SK+ K + + +A A ++P+ I AP+V
Sbjct: 344 KPSESTSHPDSLNDKTISENVQESSKDAKVDSEACQNHPTASPATVVPDQDSTITAAPSV 403
Query: 599 XSDAAAVPEIKTP 637
+ +A KTP
Sbjct: 404 TQEDSAFNTEKTP 416
>At3g20280.1 68416.m02569 PHD finger family protein contains Pfam
profile: PF00628 PHD-finger
Length = 743
Score = 29.1 bits (62), Expect = 3.4
Identities = 19/73 (26%), Positives = 33/73 (45%), Gaps = 3/73 (4%)
Frame = +2
Query: 428 KVEEPAAQPEDSKTE-VQATVAEISKEEKPSATDAEG--SADSAAIIPNMVKKIDLAPNV 598
K E + P+ + + V E SK+ K + + +A A ++P+ I AP+V
Sbjct: 605 KPSESTSHPDSLNDKTISENVQESSKDAKVDSEACQNHPTASPATVVPDQDSTITAAPSV 664
Query: 599 XSDAAAVPEIKTP 637
+ +A KTP
Sbjct: 665 TQEDSAFNTEKTP 677
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.7 bits (61), Expect = 4.5
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +2
Query: 266 DEVPAIPEAKKDDIAPE 316
D+ +IPEAK+DD APE
Sbjct: 197 DDTASIPEAKEDDAAPE 213
>At1g12080.2 68414.m01397 expressed protein
Length = 138
Score = 28.7 bits (61), Expect = 4.5
Identities = 21/81 (25%), Positives = 39/81 (48%)
Frame = +2
Query: 407 EAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVKKIDL 586
EA+ D + E+ + +D KTEV + +EEK AE ++ A++ KK ++
Sbjct: 64 EAEKKDEETEKKTEE-KDEKTEVITETPVVEEEEK----KAEEVTETPAVVEE-EKKTEV 117
Query: 587 APNVXSDAAAVPEIKTPEAAD 649
++ AA E+ +A +
Sbjct: 118 VEEKQTEVAAAEEVAVEKAEE 138
>At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containing
protein low similarity to CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 793
Score = 28.3 bits (60), Expect = 5.9
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 402 MLKQNLLILKWKNQLLSLKIQKLKYKLPSLKFQKKKNL 515
M K+N+ + K+K ++L LK K YK + K Q + L
Sbjct: 740 MEKKNMFVDKYKYRMLFLKYHKTAYKGKAPKVQSESQL 777
>At2g22450.1 68415.m02662 riboflavin biosynthesis protein, putative
similar to SP|P50855 Riboflavin biosynthesis protein
ribA [Includes: GTP cyclohydrolase II (EC 3.5.4.25);
3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP
synthase)] {Actinobacillus pleuropneumoniae}; contains
Pfam profiles PF00925: GTP cyclohydrolase II, PF00926:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Length = 476
Score = 27.9 bits (59), Expect = 7.9
Identities = 16/56 (28%), Positives = 26/56 (46%)
Frame = +2
Query: 530 EGSADSAAIIPNMVKKIDLAPNVXSDAAAVPEIKTPEAADAPKLADNPVDEDKPAD 697
EG S++ I+L P++ S E+ P D+P++ D D D+P D
Sbjct: 50 EGDVFSSSKSNGSSMGIELQPDLVSFGTLAAEM-IPTTMDSPEVEDEEFDLDRPTD 104
>At2g18050.1 68415.m02098 histone H1-3 (HIS1-3) similar to histone
H1 [Lycopersicon pennellii] SWISS-PROT:P40267; identical
to cDNA histone H1-3 (His1-3) GI:1809314, histone H1-3
[Arabidopsis thaliana] GI:1809305
Length = 167
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 4/58 (6%)
Frame = +3
Query: 360 PKKKSRKQNHPRFPMLKQNLLILKWKN----QLLSLKIQKLKYKLPSLKFQKKKNLVL 521
P+K + P F M+K+ L++LK KN ++ KI++ L F+K +L L
Sbjct: 16 PRKPKTTTHPPYFQMIKEALMVLKEKNGSSPYAIAKKIEEKHKSLLPESFRKTLSLQL 73
>At2g02170.1 68415.m00153 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 486
Score = 27.9 bits (59), Expect = 7.9
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +2
Query: 401 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 550
+AE K ++KVE + +D + AT+ E EEK +A +A+ +A
Sbjct: 411 EAEMKKTEVKVERIKGRAQDRLMKKLATI-ERKAEEKRAAAEAKKDHQAA 459
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,427,043
Number of Sequences: 28952
Number of extensions: 182692
Number of successful extensions: 609
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 581
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 606
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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