BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0133 (776 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g58470.1 68416.m06517 expressed protein several hypothetical ... 151 5e-37 At5g61720.1 68418.m07744 expressed protein predicted protein, Ar... 30 2.0 At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase fa... 30 2.0 At1g61850.1 68414.m06979 patatin family protein similar to membr... 29 2.6 At3g07160.1 68416.m00853 glycosyl transferase family 48 protein ... 29 3.4 At5g66270.1 68418.m08356 zinc finger (CCCH-type) family protein ... 28 7.9 At3g60290.1 68416.m06739 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 7.9 At1g55620.2 68414.m06367 voltage-gated chloride channel family p... 28 7.9 At1g55620.1 68414.m06366 voltage-gated chloride channel family p... 28 7.9 At1g32930.1 68414.m04056 galactosyltransferase family protein co... 28 7.9 >At3g58470.1 68416.m06517 expressed protein several hypothetical proteins - Saccharomyces cerevisiae Length = 248 Score = 151 bits (366), Expect = 5e-37 Identities = 80/215 (37%), Positives = 130/215 (60%), Gaps = 9/215 (4%) Frame = +1 Query: 136 DEDVPTLSAETFAALQEFYAEQSKRQEILVKLE--VDKKLTENI-LFDENWQLSQFWYDE 306 D+D LS++ AAL+EF A+Q+K + ++ + L E+W+LSQFWY+ Sbjct: 24 DDDPLVLSSQALAALREFLADQNKTVASTPPASSVAGGEESDKVELVTEDWRLSQFWYEP 83 Query: 307 KTVHSLVKVIDKVLDDR---GKVALISCPTLFVPLKRQIGDRGTVTLLEYDRRFEVHGPD 477 +T ++ + L R +VA I+CPTL+V LK++ V LLEYD RFE +G + Sbjct: 84 ETAETVADEV-VTLSQRIPGCRVACIACPTLYVYLKKRDPSL-QVQLLEYDMRFERYGKE 141 Query: 478 YIFYDYNNPKEVPPDVHHSYDLVVADPPFLSEECITKTSETIKLLSK---NKIILCTGTI 648 + FYDYN P+++P + H + ++VADPP+LS EC+ + S+TI L+ + ++L TG + Sbjct: 142 FTFYDYNEPEDLPLQLKHCFHIIVADPPYLSRECLERVSQTILFLASPVDSLLLLLTGEV 201 Query: 649 MKDIVKELLDLKLCEFQPKHRNNLTNEFSCYANFD 753 ++ ELL ++ C F+P H + L NEF + ++D Sbjct: 202 QREHAAELLGVRPCVFKPHHSSKLGNEFRLFISYD 236 >At5g61720.1 68418.m07744 expressed protein predicted protein, Arabidopsis thaliana Length = 390 Score = 29.9 bits (64), Expect = 2.0 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = +1 Query: 358 GKVALISCPTLFVPLKRQIGDRGTVTLLEYDRRFEVHGPDY 480 GK++L C TL V L +GT +L Y + F G DY Sbjct: 2 GKISLAICLTLLVTLSTVYETQGTFSLPLYLKNFPKLGKDY 42 >At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase SP|Q96330 {Arabidopsis thaliana}, SP|Q07512 {Petunia hybrida}; contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family Length = 357 Score = 29.9 bits (64), Expect = 2.0 Identities = 14/33 (42%), Positives = 23/33 (69%) Frame = -2 Query: 211 VFYFVQHRIPVMQQMSLLTMWARLHLLPLHKKM 113 ++ V HR+ V +++ L+ +A LH LPLHKK+ Sbjct: 280 IYKSVIHRVTVNKEVKRLS-FASLHSLPLHKKI 311 >At1g61850.1 68414.m06979 patatin family protein similar to membrane-associated calcium-independent phospholipase A2 gamma; IPLA2 gamma [Homo sapiens] GI:8453174; contains Patatin domain PF01734, PF00514: Armadillo/beta-catenin-like repeat Length = 1265 Score = 29.5 bits (63), Expect = 2.6 Identities = 21/53 (39%), Positives = 27/53 (50%) Frame = +1 Query: 121 CAMEADEDVPTLSAETFAALQEFYAEQSKRQEILVKLEVDKKLTENILFDENW 279 C ME DE P + + AA++EF QS Q V V ++LT L DE W Sbjct: 779 CGMELDETDPAIWLKLEAAIEEFI--QSNPQ---VFKNVCERLTLPFLNDEKW 826 >At3g07160.1 68416.m00853 glycosyl transferase family 48 protein similar to glucan synthase GB:AAD11794 [Filobasidiella neoformans var. neoformans] Length = 1931 Score = 29.1 bits (62), Expect = 3.4 Identities = 21/78 (26%), Positives = 38/78 (48%), Gaps = 1/78 (1%) Frame = +1 Query: 133 ADEDVPTLS-AETFAALQEFYAEQSKRQEILVKLEVDKKLTENILFDENWQLSQFWYDEK 309 A +V T+ ++ LQEFY ++ + E +K+L E+ F + +L + K Sbjct: 103 AKREVGTIDRSQDILRLQEFYRLYREKNNVDTLKEEEKQLRESGAFTD--ELERKTVKRK 160 Query: 310 TVHSLVKVIDKVLDDRGK 363 V + +KV+ VL+ K Sbjct: 161 RVFATLKVLGSVLEQLAK 178 >At5g66270.1 68418.m08356 zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 449 Score = 27.9 bits (59), Expect = 7.9 Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 6/61 (9%) Frame = +1 Query: 100 ESIATSSCAMEADEDV----PTLSAETFAALQEFYAEQSKR--QEILVKLEVDKKLTENI 261 ++ A SS + EA V P LS AAL EQ + ++LVKL D K+ EN+ Sbjct: 137 DAAAESSHSFEAPAAVSGLGPELSLLASAALSALTKEQGSQVDPDLLVKLLSDPKIVENL 196 Query: 262 L 264 + Sbjct: 197 I 197 >At3g60290.1 68416.m06739 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase 1 [SP|Q96330], gibberellin 20-oxidase [GI:9791186]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain Length = 316 Score = 27.9 bits (59), Expect = 7.9 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = -2 Query: 211 VFYFVQHRIPVMQQMSLLTMWARLHLLPLHKKM 113 ++ V HR+ V + L+ +A LH LP+HKK+ Sbjct: 239 IYKSVVHRVTVNKDYKRLS-FASLHSLPMHKKI 270 >At1g55620.2 68414.m06367 voltage-gated chloride channel family protein contains Pfam profiles PF00654: Voltage gated chloride channel, PF00571: CBS domain Length = 781 Score = 27.9 bits (59), Expect = 7.9 Identities = 14/46 (30%), Positives = 28/46 (60%), Gaps = 4/46 (8%) Frame = +1 Query: 532 SYDLVVADPP----FLSEECITKTSETIKLLSKNKIILCTGTIMKD 657 S +L V + P FL EE I + + ++++SKN + + +GT +++ Sbjct: 591 SLELTVIENPDHNSFLDEETILEDLKVMRVMSKNYVKVSSGTTLRE 636 >At1g55620.1 68414.m06366 voltage-gated chloride channel family protein contains Pfam profiles PF00654: Voltage gated chloride channel, PF00571: CBS domain Length = 585 Score = 27.9 bits (59), Expect = 7.9 Identities = 14/46 (30%), Positives = 28/46 (60%), Gaps = 4/46 (8%) Frame = +1 Query: 532 SYDLVVADPP----FLSEECITKTSETIKLLSKNKIILCTGTIMKD 657 S +L V + P FL EE I + + ++++SKN + + +GT +++ Sbjct: 395 SLELTVIENPDHNSFLDEETILEDLKVMRVMSKNYVKVSSGTTLRE 440 >At1g32930.1 68414.m04056 galactosyltransferase family protein contains Pfam profile: PF01762 galactosyltransferase Length = 399 Score = 27.9 bits (59), Expect = 7.9 Identities = 12/27 (44%), Positives = 15/27 (55%) Frame = +3 Query: 150 HIVSRDICCITGILC*TK*KTRNPCKA 230 HI R +CC T + C K + NPC A Sbjct: 338 HIDDRSLCCGTPLDCEWKGQAGNPCAA 364 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,377,114 Number of Sequences: 28952 Number of extensions: 341182 Number of successful extensions: 979 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 948 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 975 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1736283200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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