Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0127
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 151 1e-37
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 55 9e-09
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 50 4e-07
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 49 6e-07
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 49 6e-07
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 43 5e-05
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 41 2e-04
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 39 8e-04
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 38 0.001
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 37 0.002
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 36 0.006
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 34 0.017
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 34 0.023
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 28 1.5
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.5
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 28 1.5
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.6
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 4.6
SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 26 6.1
SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 25 8.1
SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch... 25 8.1
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 151 bits (365), Expect = 1e-37
Identities = 65/135 (48%), Positives = 96/135 (71%)
Frame = +3
Query: 153 VPRNFRLL*ELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIE 332
VPRNF+LL ELE+G+KG+G+ + S+GL + DD+TL+ W I+GP + +ENR+YSLKI
Sbjct: 4 VPRNFKLLEELEKGEKGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIH 63
Query: 333 CGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQVPILARWQRDYTIKTVLQEVRRLMTL 512
C YPD PP F+SRI++ V+ +TG V+ ++ L W+R+Y+++TVL ++++ M
Sbjct: 64 CDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKKEMAS 123
Query: 513 KDNMKLSQPPEGSLF 557
N KL QPPEGS F
Sbjct: 124 SSNRKLPQPPEGSTF 138
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 55.2 bits (127), Expect = 9e-09
Identities = 38/117 (32%), Positives = 58/117 (49%), Gaps = 1/117 (0%)
Frame = +3
Query: 159 RNFRLL*ELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECG 338
R+ R+ EL Q+ G W + D ++H GM GP TPYE + + IE
Sbjct: 5 RSRRIAKELADVQQDKQAGIQVWTINDD----ISHLKGMFRGPEGTPYEGGYFVVDIEIP 60
Query: 339 TRYPDEPPTARFISRIHMNCVNSQTGLVDNRQVPILA-RWQRDYTIKTVLQEVRRLM 506
YP PP F ++I+ V+SQTG + + IL +W YT+K+ L ++ L+
Sbjct: 61 IDYPFRPPKMNFDTKIYHPNVSSQTGAI---CLDILKDQWSPVYTMKSALISLQSLL 114
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 49.6 bits (113), Expect = 4e-07
Identities = 29/95 (30%), Positives = 43/95 (45%), Gaps = 1/95 (1%)
Frame = +3
Query: 240 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQTG 416
D D L HW G I GP T YE + + + YP PPT F S + H N S
Sbjct: 53 DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNI 112
Query: 417 LVDNRQVPILARWQRDYTIKTVLQEVRRLMTLKDN 521
+D + +W Y ++T+L ++ L+ +N
Sbjct: 113 CLD----ILKDKWSAVYNVQTILLSLQSLLGEPNN 143
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 49.2 bits (112), Expect = 6e-07
Identities = 25/87 (28%), Positives = 44/87 (50%), Gaps = 1/87 (1%)
Frame = +3
Query: 264 WTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQTGLVDNRQVP 440
W +IIGP TP+E+ + L + +YP++PP +F+S + H N + +D Q
Sbjct: 36 WNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQ-- 93
Query: 441 ILARWQRDYTIKTVLQEVRRLMTLKDN 521
RW Y + +L ++ L+ +N
Sbjct: 94 --NRWSPTYDVAAILTSIQSLLNDPNN 118
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 49.2 bits (112), Expect = 6e-07
Identities = 29/86 (33%), Positives = 42/86 (48%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 434
L HW I+GP +PY ++ L I T YP +PP F +RI+ +NS +
Sbjct: 30 LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSIC---- 85
Query: 435 VPILA-RWQRDYTIKTVLQEVRRLMT 509
+ IL +W TI VL + L+T
Sbjct: 86 LDILRDQWSPALTISKVLLSICSLLT 111
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 42.7 bits (96), Expect = 5e-05
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +3
Query: 246 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 395
D+ + HW +I GP TPYE + L I YP PP+ F ++I H N
Sbjct: 31 DVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPN 81
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 41.1 bits (92), Expect = 2e-04
Identities = 30/104 (28%), Positives = 47/104 (45%), Gaps = 1/104 (0%)
Frame = +3
Query: 237 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQT 413
SD + L +W I G P+T +E +Y L + YP PP RF + H N S
Sbjct: 32 SDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPS-- 89
Query: 414 GLVDNRQVPILARWQRDYTIKTVLQEVRRLMTLKDNMKLSQPPE 545
G V + W+ TIK +L ++ L+ D+ ++ P +
Sbjct: 90 GTVCLSILNEEEGWKPAITIKQILLGIQDLL---DDPNIASPAQ 130
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 38.7 bits (86), Expect = 8e-04
Identities = 23/69 (33%), Positives = 37/69 (53%)
Frame = +3
Query: 168 RLL*ELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 347
RL+ E ++ + DG I+ G ++DD W +I GP TP+E +Y ++ + Y
Sbjct: 9 RLMKEYKELTENGPDG-ITAGPSNEDDFFT--WDCLIQGPDGTPFEGGLYPATLKFPSDY 65
Query: 348 PDEPPTARF 374
P PPT +F
Sbjct: 66 PLGPPTLKF 74
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 37.9 bits (84), Expect = 0.001
Identities = 26/73 (35%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = +3
Query: 180 ELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEP 359
+L++ QK G S GL DD ++ W MIIGP T YE + + YP P
Sbjct: 13 QLKEIQKNPPQG-FSVGLV--DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMP 69
Query: 360 PTARFISRI-HMN 395
P +F + I H N
Sbjct: 70 PKMKFTTEIWHPN 82
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 37.1 bits (82), Expect = 0.002
Identities = 24/85 (28%), Positives = 36/85 (42%)
Frame = +3
Query: 255 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 434
+ W +I GPP TPYE Y + YP +PP R I+ N++ L +
Sbjct: 34 ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITPSGRFQTNTRLCLSFSDF 93
Query: 435 VPILARWQRDYTIKTVLQEVRRLMT 509
P W + + T+L + MT
Sbjct: 94 HP--KSWNPSWMVSTILVGLVSFMT 116
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 35.9 bits (79), Expect = 0.006
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +3
Query: 246 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 389
D L HW +GP + Y + ++ YP +PPT F +RI+
Sbjct: 33 DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 34.3 bits (75), Expect = 0.017
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +3
Query: 282 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 422
GP TPY ++ + +E + YP + P+ F++RI ++ +G V
Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 33.9 bits (74), Expect = 0.023
Identities = 20/75 (26%), Positives = 36/75 (48%)
Frame = +3
Query: 282 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQVPILARWQR 461
GP ++ YE + L++ YP PP RF+++I+ V+ + + + W
Sbjct: 40 GPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLS---TLKKDWSP 96
Query: 462 DYTIKTVLQEVRRLM 506
I+TVL ++ LM
Sbjct: 97 ALQIRTVLLSIQALM 111
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 27.9 bits (59), Expect = 1.5
Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Frame = +3
Query: 162 NFRLL*ELEQGQKGVGDGTISWGLESDDDMTLTHW----TGMIIGPPRTPYENRMYSLKI 329
++RLL E+ G +WG+ +DD ++ W +G I+ +TPYE M L+
Sbjct: 340 SYRLLPVEERNISCSGRDDFAWGILNDDWVSHPTWASEESGFIV-QRKTPYEEAMTKLEE 398
Query: 330 E 332
E
Sbjct: 399 E 399
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -3
Query: 452 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 357
S+ NW S+++ SL D + NP+NK W
Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322
>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 584
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = -3
Query: 272 SSPMSKSHIIIAFKTPRNGSIA 207
SSPMS+SH+ I+ + PR G A
Sbjct: 439 SSPMSESHVQISSEMPRTGMSA 460
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -2
Query: 93 KLRLIQSSFIA*ALPKQIRFI*SKYGNLQ 7
K RL++S F +LP Q+RF+ Y NL+
Sbjct: 27 KFRLLESQFALYSLPCQLRFV--HYNNLE 53
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 26.2 bits (55), Expect = 4.6
Identities = 9/37 (24%), Positives = 19/37 (51%)
Frame = +3
Query: 279 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 389
I P Y+ + +I+ YP +PP + +++I+
Sbjct: 58 IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94
>SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 543
Score = 25.8 bits (54), Expect = 6.1
Identities = 19/50 (38%), Positives = 28/50 (56%), Gaps = 4/50 (8%)
Frame = +1
Query: 76 LN*SKFL--QFSKPV--IL*SWRIRHQALWCHGIFVSYKNLNKAKREWAM 213
LN KF +FSKP+ + +W + AL +F SY+N NK + WA+
Sbjct: 438 LNPGKFYCGKFSKPISIVAVAW-LWFMALML--LFPSYQNPNKVEMNWAI 484
>SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1692
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +3
Query: 474 KTVLQEVRRLMTLKDNMKLSQPPEGSLF*FLQSSLNRPNLLSH 602
K+ L+ ++ L + N K S P L F+Q + +PN+L +
Sbjct: 954 KSYLKNLKFLSIMDLNTKFSNAPSDVLNHFIQRNSPQPNILRY 996
>SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +3
Query: 519 NMKLSQPPEGSLF*FLQSSLNRPNLLSHKTIILK*IK 629
N+++ PP G LQ S PN T+I + IK
Sbjct: 578 NLEIQSPPPGVFIFRLQESFTYPNASRVSTMISRRIK 614
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,942,224
Number of Sequences: 5004
Number of extensions: 61443
Number of successful extensions: 154
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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