Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0117
(623 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g08310.1 68417.m01372 expressed protein glutamic acid-rich pr... 32 0.36
At4g19230.2 68417.m02837 cytochrome P450 family protein cytochro... 31 0.47
At5g17930.1 68418.m02102 MA3 domain-containing protein low simil... 31 0.82
At3g19900.1 68416.m02520 expressed protein 29 1.9
At5g17240.1 68418.m02020 SET domain-containing protein contains ... 29 2.5
At4g02710.1 68417.m00366 kinase interacting family protein simil... 29 3.3
At3g20860.1 68416.m02637 protein kinase family protein contains ... 29 3.3
At5g64710.2 68418.m08135 expressed protein contains Pfam profile... 28 4.4
At5g64710.1 68418.m08134 expressed protein contains Pfam profile... 28 4.4
At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 28 4.4
At3g04810.2 68416.m00521 protein kinase, putative similar to LST... 28 4.4
At3g04810.1 68416.m00520 protein kinase, putative similar to LST... 28 4.4
At1g66130.1 68414.m07505 oxidoreductase N-terminal domain-contai... 28 4.4
At5g28290.1 68418.m03434 protein kinase, putative similar to LST... 28 5.8
At2g26780.1 68415.m03212 expressed protein contains Pfam profile... 27 7.7
At2g04230.1 68415.m00410 F-box family protein contains F-box dom... 27 7.7
>At4g08310.1 68417.m01372 expressed protein glutamic acid-rich
protein precursor - Plasmodium falciparum, PIR2:A54514
Length = 504
Score = 31.9 bits (69), Expect = 0.36
Identities = 21/59 (35%), Positives = 30/59 (50%)
Frame = +1
Query: 73 DEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLIKSQLMTKDGK 249
D K +K+H CL+ + DE N L+T + K + P+K+ A L K KDGK
Sbjct: 77 DVHKSFVKQHLVQCLAGAENDETSENSLET-EKKDDVTPVKEAA--ELSKEHTTKKDGK 132
>At4g19230.2 68417.m02837 cytochrome P450 family protein cytochrome
P450, Arabidopsis thaliana; supported by cDNA:
gi_15293092_gb_AY050980.1_
Length = 484
Score = 31.5 bits (68), Expect = 0.47
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -2
Query: 409 ERASGLSRGSISHNSRLFGEGCCLCWPGKRQS 314
ERA+G S G +SR C LCWPG R+S
Sbjct: 439 ERATGFSMG----HSRFPKTDCPLCWPGSRRS 466
>At5g17930.1 68418.m02102 MA3 domain-containing protein low
similarity to SP|Q9P6R9 Cell cycle control protein cwf22
{Schizosaccharomyces pombe}; contains Pfam profile
PF02847: MA3 domain
Length = 707
Score = 30.7 bits (66), Expect = 0.82
Identities = 25/104 (24%), Positives = 47/104 (45%), Gaps = 2/104 (1%)
Frame = +1
Query: 106 ADCLSETKADE--QLVNKLKTGDFKTENEPLKKYALCMLIKSQLMTKDGKFKKDVALAKV 279
A+ ++ET E + LK + + N +K C+++ S+ F+K + L +
Sbjct: 479 AEDIAETMDAEVVEAQKMLKLAEAQRMNTDSRKAIFCVIMSSEDYID--AFEKLLRL-DL 535
Query: 280 PNAEDKLKVEKLIDACLANKGNSPHQTAWNYVKCYHEKDPKHAL 411
P +D+ + L++ CL K + T C H+K+ K L
Sbjct: 536 PGKQDREIMRVLVECCLQEKAFNKFYTVLASKLCEHDKNHKFTL 579
>At3g19900.1 68416.m02520 expressed protein
Length = 222
Score = 29.5 bits (63), Expect = 1.9
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = -3
Query: 159 LQFVHQLFISFRLGETVGSVFLQVLLLLICEGLSQDNADDEHDKSLHD 16
L F H L + G+T F ++ ++ EG + DN+DDE +SL D
Sbjct: 42 LAFSHSLCLKTTYGKTDRRKFSRICSIVDDEG-NLDNSDDEEKESLDD 88
>At5g17240.1 68418.m02020 SET domain-containing protein contains
Pfam profile PF00856: SET domain
Length = 491
Score = 29.1 bits (62), Expect = 2.5
Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 190 LKKYALCMLI-KSQLMTKDGKFKKDVALAKVPNAEDKLKVEKLIDACLANKGNSPHQTAW 366
LKK L + + + LMT + KD+ L+ N + L +++ CL + + ++ W
Sbjct: 57 LKKGELVLKVPRKALMTTESIIAKDLKLSDAVNLHNSLSSTQILSVCLLYEMSKEKKSFW 116
Query: 367 NYVKCYH 387
Y +H
Sbjct: 117 -YPYLFH 122
>At4g02710.1 68417.m00366 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1111
Score = 28.7 bits (61), Expect = 3.3
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = -2
Query: 430 DYVFTRKERASGLSRGSISHNSRLFGEGCCL 338
DYVFT +E A +S+G+ + L EG CL
Sbjct: 781 DYVFTHRESAGEVSKGADLMDEFLKLEGMCL 811
>At3g20860.1 68416.m02637 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 427
Score = 28.7 bits (61), Expect = 3.3
Identities = 23/85 (27%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
Frame = +1
Query: 43 VCVVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 222
VC+V + + + +KK R SE K +V L D+ N L + C I
Sbjct: 87 VCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAIDYLHNNRVLHRDLKCSNI- 145
Query: 223 SQLMTKDGKFK-KDVALAKVPNAED 294
+TK+ + + D LAK+ +D
Sbjct: 146 --FLTKENEVRLGDFGLAKLLGKDD 168
>At5g64710.2 68418.m08135 expressed protein contains Pfam profile
PF04396: Protein of unknown function, DUF537
Length = 716
Score = 28.3 bits (60), Expect = 4.4
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 49 VVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLI--- 219
VV + + K+N K + + ++ ++ V +K D K + EP+K L +
Sbjct: 237 VVSVEKMCQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQLAITAVDD 296
Query: 220 KSQLMTKDGKFKK 258
S KDG KK
Sbjct: 297 VSSFEEKDGFLKK 309
>At5g64710.1 68418.m08134 expressed protein contains Pfam profile
PF04396: Protein of unknown function, DUF537
Length = 841
Score = 28.3 bits (60), Expect = 4.4
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +1
Query: 49 VVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLI--- 219
VV + + K+N K + + ++ ++ V +K D K + EP+K L +
Sbjct: 362 VVSVEKMCQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQLAITAVDD 421
Query: 220 KSQLMTKDGKFKK 258
S KDG KK
Sbjct: 422 VSSFEEKDGFLKK 434
>At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain,
PF04937: Protein of unknown function (DUF 659)
Length = 433
Score = 28.3 bits (60), Expect = 4.4
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +1
Query: 100 HRADCLSETKADEQLVNKLKT-GDFKTENEPLKKYALCMLIKSQLMTKD-GKFKKDVALA 273
H D + E + + K++T GD+K EN L +Y C+L + +D G K + LA
Sbjct: 227 HTVDLMLEAISKLPKMGKMQTFGDYKGENLFLNRYESCVLEWCDVCVEDFGPLMKLLRLA 286
Query: 274 KVPN 285
N
Sbjct: 287 DGEN 290
>At3g04810.2 68416.m00521 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 578
Score = 28.3 bits (60), Expect = 4.4
Identities = 23/85 (27%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
Frame = +1
Query: 43 VCVVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 222
VC+++ + E +KK +E K + LV L ++ N L + C I
Sbjct: 77 VCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYLHANHILHRDVKCSNI- 135
Query: 223 SQLMTKDGKFK-KDVALAKVPNAED 294
+TKD + D LAKV ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKVLTSDD 158
>At3g04810.1 68416.m00520 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 606
Score = 28.3 bits (60), Expect = 4.4
Identities = 23/85 (27%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
Frame = +1
Query: 43 VCVVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 222
VC+++ + E +KK +E K + LV L ++ N L + C I
Sbjct: 77 VCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYLHANHILHRDVKCSNI- 135
Query: 223 SQLMTKDGKFK-KDVALAKVPNAED 294
+TKD + D LAKV ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKVLTSDD 158
>At1g66130.1 68414.m07505 oxidoreductase N-terminal
domain-containing protein similar to AX110P [Daucus
carota] GI:285739; contains Pfam profile PF01408:
Oxidoreductase family NAD-binding Rossmann fold
Length = 364
Score = 28.3 bits (60), Expect = 4.4
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 253 KKDVALAKVPNAEDKLKVEKLIDACLANKGNSPHQTAW 366
KK L + P A+D ++EK+++AC N T W
Sbjct: 95 KKKHVLVEKPPAQDATELEKIVEACEYNGVQFMDGTIW 132
>At5g28290.1 68418.m03434 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 568
Score = 27.9 bits (59), Expect = 5.8
Identities = 23/85 (27%), Positives = 37/85 (43%), Gaps = 1/85 (1%)
Frame = +1
Query: 43 VCVVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYALCMLIK 222
VC+V+ + E +KK SE K + LV L ++ + L + C I
Sbjct: 77 VCIVIGYCKGGDMAEAIKKANGVEFSEEKLCKWLVQLLMALEYLHASHILHRDVKCSNI- 135
Query: 223 SQLMTKDGKFK-KDVALAKVPNAED 294
+TKD + D LAK+ ++D
Sbjct: 136 --FLTKDQDIRLGDFGLAKILTSDD 158
>At2g26780.1 68415.m03212 expressed protein contains Pfam profile
TBP (TATA-binding protein) -interacting protein 120
(TIP120); contains TIGRFAM profile TIGR01612:
reticulocyte binding protein
Length = 1866
Score = 27.5 bits (58), Expect = 7.7
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +3
Query: 321 RLPGQQRQQPSPNSLELCEMLPRERPE 401
R P ++R++ +PN+LE LP++ E
Sbjct: 114 RAPAKEREEIAPNTLENVSKLPKQHQE 140
>At2g04230.1 68415.m00410 F-box family protein contains F-box domain
Pfam:PF00646
Length = 448
Score = 27.5 bits (58), Expect = 7.7
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 283 NAEDKLKVEKLIDACLANKGNSPHQTAWNYVKCYHE 390
++ KL++ KL D L + +P + WN KC E
Sbjct: 333 DSSPKLQILKLTDVYLHDNKTNPDERKWNPPKCAPE 368
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,247,209
Number of Sequences: 28952
Number of extensions: 274869
Number of successful extensions: 766
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 749
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 765
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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