Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0113
(665 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 343 6e-95
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 343 6e-95
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 343 6e-95
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 343 6e-95
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 191 3e-49
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 190 5e-49
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 100 8e-22
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 95 4e-20
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 2e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 2e-08
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 52 3e-07
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 5e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 51 6e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 47 1e-05
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 43 2e-04
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 43 2e-04
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 5e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 5e-04
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 41 9e-04
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 40 0.001
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 36 0.018
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 36 0.018
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 35 0.042
At5g46180.1 68418.m05680 ornithine aminotransferase, putative / ... 31 0.91
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 31 0.91
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.6
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.6
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 29 2.1
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 29 2.1
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.8
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 4.9
At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase fa... 28 6.4
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 8.5
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 8.5
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 343 bits (843), Expect = 6e-95
Identities = 161/199 (80%), Positives = 177/199 (88%)
Frame = +3
Query: 69 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 248
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 249 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGT 428
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTS ADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 429 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 608
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 609 IGYNPAAVAFVPISGWHGN 665
+GYNP + FVPISG+ G+
Sbjct: 181 VGYNPDKIPFVPISGFEGD 199
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 343 bits (843), Expect = 6e-95
Identities = 161/199 (80%), Positives = 177/199 (88%)
Frame = +3
Query: 69 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 248
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 249 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGT 428
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTS ADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 429 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 608
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 609 IGYNPAAVAFVPISGWHGN 665
+GYNP + FVPISG+ G+
Sbjct: 181 VGYNPDKIPFVPISGFEGD 199
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 343 bits (843), Expect = 6e-95
Identities = 161/199 (80%), Positives = 177/199 (88%)
Frame = +3
Query: 69 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 248
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 249 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGT 428
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTS ADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 429 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 608
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 609 IGYNPAAVAFVPISGWHGN 665
+GYNP + FVPISG+ G+
Sbjct: 181 VGYNPDKIPFVPISGFEGD 199
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 343 bits (843), Expect = 6e-95
Identities = 161/199 (80%), Positives = 177/199 (88%)
Frame = +3
Query: 69 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 248
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 249 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGT 428
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTS ADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 429 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 608
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 609 IGYNPAAVAFVPISGWHGN 665
+GYNP + FVPISG+ G+
Sbjct: 181 VGYNPDKIPFVPISGFEGD 199
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 191 bits (466), Expect = 3e-49
Identities = 90/196 (45%), Positives = 133/196 (67%), Gaps = 1/196 (0%)
Frame = +3
Query: 78 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 257
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 258 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEF 437
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G S AD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 438 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGY 617
E G + GQTREH LA TLGV +LIV VNKMD +S+ R++EI++++ ++K GY
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGY 277
Query: 618 NPAA-VAFVPISGWHG 662
N V F+PISG G
Sbjct: 278 NTKKDVVFLPISGLMG 293
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 190 bits (464), Expect = 5e-49
Identities = 85/190 (44%), Positives = 132/190 (69%), Gaps = 1/190 (0%)
Frame = +3
Query: 84 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 263
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 264 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEA 443
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G + AD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 444 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 620
G GQTREHA + GV+Q+IV +NKMD YS+ RF+ IK+ V S+++ +
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFK 415
Query: 621 PAAVAFVPIS 650
+++ ++P+S
Sbjct: 416 DSSLTWIPLS 425
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 100 bits (240), Expect = 8e-22
Identities = 62/179 (34%), Positives = 92/179 (51%), Gaps = 1/179 (0%)
Frame = +3
Query: 66 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 245
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 246 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAG 425
+D ER RGITI+ A ++ET + +D PGH D++KNMITG + D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 426 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE-EIKKEVSSY 599
G QT+EH LLA +GV ++V +NK D + E E+++ +SSY
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 95.1 bits (226), Expect = 4e-20
Identities = 61/177 (34%), Positives = 89/177 (50%)
Frame = +3
Query: 75 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 254
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 255 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGE 434
E++RGITI A ++ET+K + +D PGH D++KNMITG + D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 435 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 605
QT+EH LLA +GV L+ +NK+D + P E +E+ S+ K
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYK 217
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 56.0 bits (129), Expect = 2e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 273 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGIS 452
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 453 KNGQTREH 476
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 56.0 bits (129), Expect = 2e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 273 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGIS 452
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 453 KNGQTREH 476
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 52.0 bits (119), Expect = 3e-07
Identities = 52/174 (29%), Positives = 81/174 (46%), Gaps = 4/174 (2%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 273 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFE 440
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190
Query: 441 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 602
A QT + LA + ++I +NK+D P +EP E++ +E+ I
Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVI 232
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 51.6 bits (118), Expect = 5e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 273 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTG 431
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.2 bits (117), Expect = 6e-07
Identities = 33/94 (35%), Positives = 49/94 (52%)
Frame = +3
Query: 81 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 260
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 261 AERERGITIDIALWKFETSKYYVTIIDAPGHRDF 362
E+ERGITI A K+ + IID PGH DF
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDF 174
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 47.2 bits (107), Expect = 1e-05
Identities = 57/186 (30%), Positives = 79/186 (42%), Gaps = 9/186 (4%)
Frame = +3
Query: 66 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 245
K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 60 KFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY--- 102
Query: 246 LDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTSHAD 398
LDKL +RERGIT+ E S Y + +ID PGH DF + S
Sbjct: 103 LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQ 160
Query: 399 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 578
A+L+V A G QT + LAF + ++ +NK+D P ++P E +
Sbjct: 161 GALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKIDQ---PTADP--ERV 207
Query: 579 KKEVSS 596
K ++ S
Sbjct: 208 KAQLKS 213
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 42.7 bits (96), Expect = 2e-04
Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%)
Frame = +3
Query: 63 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 233
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 234 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 380
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 381 GTSHADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 560
G + D A+L++AA QT EH + +K +I+ NK+D +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200
Query: 561 PRFEEIKKEVSS 596
+ E I+K +++
Sbjct: 201 EQHEAIQKFITN 212
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 42.7 bits (96), Expect = 2e-04
Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%)
Frame = +3
Query: 63 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 233
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 234 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 380
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 381 GTSHADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 560
G + D A+L++AA QT EH + +K +I+ NK+D +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200
Query: 561 PRFEEIKKEVSS 596
+ E I+K +++
Sbjct: 201 EQHEAIQKFITN 212
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.5 bits (93), Expect = 5e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 273 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGIS 452
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185
Query: 453 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 569
QTR A G ++V VNK+D P + P F
Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.5 bits (93), Expect = 5e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 273 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGIS 452
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184
Query: 453 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 569
QTR A G ++V VNK+D P + P F
Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 40.7 bits (91), Expect = 9e-04
Identities = 44/186 (23%), Positives = 82/186 (44%), Gaps = 8/186 (4%)
Frame = +3
Query: 63 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 224
P++ + INI IGHV GKST +G H + +++ TI+ A+
Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84
Query: 225 SFKYAWVLDKLKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSHAD 398
+ +E + D++ + K +V+ +D PGH + M+ G + D
Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144
Query: 399 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 578
A+LI+AA QT EH + +K +I+ NK+D + + + E+I
Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKIDLIQENEAIKQHEDI 198
Query: 579 KKEVSS 596
++ +++
Sbjct: 199 QRFITN 204
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 40.3 bits (90), Expect = 0.001
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +3
Query: 306 FETSKY----YVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGISKNGQTRE 473
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 474 HALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 602
H + +K +I+ NK+D + + + E I+K + + +
Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.1 bits (87), Expect = 0.003
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 260
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 261 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIV 416
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.3 bits (80), Expect = 0.018
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 273 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTG 431
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.3 bits (80), Expect = 0.018
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 93 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 272
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 273 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTG 431
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 35.1 bits (77), Expect = 0.042
Identities = 33/105 (31%), Positives = 50/105 (47%), Gaps = 3/105 (2%)
Frame = +3
Query: 327 VTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 506
+T +D PGH F + G + D VL+VAA G QT E A + V
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321
Query: 507 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSS---YIKKIGYNPAAV 632
++V +NK D P + P E++K +++S ++ IG N AV
Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTSEGIELEDIGGNVQAV 361
>At5g46180.1 68418.m05680 ornithine aminotransferase, putative /
ornithine--oxo-acid aminotransferase, putative similar
to SP|Q92413 Ornithine aminotransferase (EC 2.6.1.13)
(Ornithine--oxo-acid aminotransferase) [Aspergillus
nidulans] {Emericella nidulans}; contains Pfam profile
PF00202: aminotransferase, class III
Length = 475
Score = 30.7 bits (66), Expect = 0.91
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +3
Query: 492 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISG 653
T GV++ G+ + +S PP S R E++ E S++ Y+P V F +G
Sbjct: 18 TAGVRRSYGGLPQSNSKSPPSSSQRLMELESEFSAH----NYHPVPVVFSRANG 67
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 30.7 bits (66), Expect = 0.91
Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 1/96 (1%)
Frame = +3
Query: 336 IDAPGHRDFIKNMITGTSHADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 512
I GH D+ + T +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 513 IVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 620
+ +DS E +E+++E S+ K++G N
Sbjct: 286 NLSTFTLDSDEE-------DEVREE-SNVAKEVGLN 313
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.6
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +3
Query: 333 IIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 512
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 513 IVGVNKMD 536
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.9 bits (64), Expect = 1.6
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +3
Query: 327 VTIIDAPGHRDFIKNMITGTSHADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 506
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 507 QLIVGVNKMD 536
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 333 IIDAPGHRDFIKNMITGTSHADCAVLIV 416
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 333 IIDAPGHRDFIKNMITGTSHADCAVLIV 416
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.1 bits (62), Expect = 2.8
Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%)
Frame = +3
Query: 252 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVA 419
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 420 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 536
A G QT E A+ +++ +NK+D
Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +3
Query: 555 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 644
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase
family protein similar to Potential
phospholipid-transporting ATPase (EC 3.6.3.1) from Homo
sapiens [SP|Q9Y2Q0, SP|O43520], Mus musculus [SP|P98200,
SP|P70704], {Bos taurus} SP|Q29449; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 1184
Score = 27.9 bits (59), Expect = 6.4
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +3
Query: 438 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 596
E+G +TR+H G++ LI+ ++D E R E K VS+
Sbjct: 629 ESGRKYEKETRDHVNEYADAGLRTLILAYRELDENEYEVFTERISEAKNSVSA 681
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 8.5
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 537 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 650
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 8.5
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 537 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 650
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,889,642
Number of Sequences: 28952
Number of extensions: 306093
Number of successful extensions: 967
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 918
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 952
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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