BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0098 (671 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 100 2e-22 SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 39 8e-04 SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.46 SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo... 29 0.61 SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 3.3 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 5.7 SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 7.5 SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 25 7.5 SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 7.5 SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 25 9.9 SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|... 25 9.9 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 9.9 >SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|||Manual Length = 137 Score = 100 bits (240), Expect = 2e-22 Identities = 55/140 (39%), Positives = 82/140 (58%) Frame = +1 Query: 67 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 246 SGV VS C ++E+K K RYVVF + D K V + +++ FL DL + Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60 Query: 247 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 426 +CRY ++DFE+ + +G + K+ +SW PD A +K KM+YSSS D L+++ G+ Sbjct: 61 DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114 Query: 427 KYIQATDLSEASHEAVEEKL 486 IQATD SE ++E V EK+ Sbjct: 115 TDIQATDFSEVAYETVLEKV 134 >SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 38.7 bits (86), Expect = 8e-04 Identities = 37/137 (27%), Positives = 63/137 (45%), Gaps = 2/137 (1%) Frame = +1 Query: 133 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 312 R + I +E DV+T+ E++ E D +K EC G + + + S Sbjct: 26 RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76 Query: 313 KKQKLFLMSWCPDTAKVKKKMLYSSSFDALKK--SLVGVQKYIQATDLSEASHEAVEEKL 486 KK L L+S+ P+ A V++KMLY+SS A + +L + + A+ E ++ + + L Sbjct: 77 KKNLLQLISYVPENANVRRKMLYASSRAAFVRCVTLAKLDESYFASTPEELDYQQIMKSL 136 Query: 487 RATDRQ*TAFTHELATK 537 + Q EL K Sbjct: 137 SKQEDQSPLRQDELERK 153 Score = 27.9 bits (59), Expect = 1.4 Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 5/56 (8%) Frame = +1 Query: 322 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASHEAV 474 K+ + CP A VK +M+YSSS D++K L + + I++ D ++ + + + Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299 >SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 314 Score = 29.5 bits (63), Expect = 0.46 Identities = 40/198 (20%), Positives = 78/198 (39%), Gaps = 3/198 (1%) Frame = +1 Query: 40 LRE*HQKMASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGE-RNAEYEQF 216 LR ++ G D+ ++ +E ++KK + F RD++++ ++ R Sbjct: 41 LRSEGSELIFGGRSFDSLLSSIKETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTPRNRTQ 100 Query: 217 LEDLQ--KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSS 390 + D + + T +F+ Q T A+K + P T K + ++ Sbjct: 101 IIDARSTRKDTENESLRKNNFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAE 160 Query: 391 FDALKKSLVGVQKYIQATDLSEASHEAVEEKLRATDRQ*TAFTHELATKPNPLSDTPALT 570 A KK + G +KY + S ++ E K R DR + FT LS ++ Sbjct: 161 RPASKKPVFGSKKYFDVINDSNVENKE-ETKDRNLDRALSKFTQSREKNEQNLSSKASVL 219 Query: 571 TRGHDTTSRLVLLQRENE 624 + +LL++ N+ Sbjct: 220 -----RNKKSILLKKRNQ 232 >SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 29.1 bits (62), Expect = 0.61 Identities = 17/63 (26%), Positives = 30/63 (47%) Frame = +1 Query: 298 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASHEAVE 477 T++ LF++ W P M+Y+S+ + + V K +A D + + EAV+ Sbjct: 79 TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137 Query: 478 EKL 486 E L Sbjct: 138 EFL 140 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 26.6 bits (56), Expect = 3.3 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 388 SFDALKKSLVGVQKYIQATDLSEAS 462 S +AL++ L G + Y+Q+TD++ S Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMS 212 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.8 bits (54), Expect = 5.7 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%) Frame = -1 Query: 341 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 225 HD+ +++ S +H C ++S P+ LH+P+P C+ Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150 >SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor complex subunit Rrn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 25.4 bits (53), Expect = 7.5 Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 4/92 (4%) Frame = -1 Query: 557 VSESGFGFVASSCVNAVYWRSVARSFSSTAS*DASERSVAWMYF*TPTR---DFFRASNE 387 + E +G ++ SCV+ YW + + A +R + + + P++ R N Sbjct: 56 LEEEQWGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINA 115 Query: 386 LEYNIFFLTLAVSGHHDIR-KSFCFLLASDVP 294 LE + +L H D +S C L D+P Sbjct: 116 LENELRWL----RNHVDASVRSQCLLKYEDIP 143 >SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr 2|||Manual Length = 319 Score = 25.4 bits (53), Expect = 7.5 Identities = 14/61 (22%), Positives = 31/61 (50%) Frame = +1 Query: 94 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 273 K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166 Query: 274 E 276 + Sbjct: 167 Q 167 >SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 357 Score = 25.4 bits (53), Expect = 7.5 Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%) Frame = -1 Query: 293 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 162 W++ Y+ S R Y VPP+CR +R+ + CF Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167 >SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 366 Score = 25.0 bits (52), Expect = 9.9 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 76 TVSDACKTTYEEIKKDKKHRYV 141 TV D C + IK KH YV Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337 >SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/35 (40%), Positives = 19/35 (54%) Frame = +1 Query: 73 VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV 177 VT D KT + +K KH+ V YIR K +D+ Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVDI 146 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/39 (35%), Positives = 17/39 (43%) Frame = +3 Query: 483 APRHRSPINSIYTRARDETEPALRHSCPDDTRPRHH*PL 599 APR R P ++ T A E P + P TR PL Sbjct: 24 APRKREPARTVSTPAFMEPAPVSKKPLPPPTRRLPRKPL 62 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,351,825 Number of Sequences: 5004 Number of extensions: 43869 Number of successful extensions: 145 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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