BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0093 (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC31G5.16c |dpm1||dolichol-phosphate mannosyltransferase catal... 281 5e-77 SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharo... 28 1.0 SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 26 5.5 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 26 5.5 SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|c... 26 5.5 >SPAC31G5.16c |dpm1||dolichol-phosphate mannosyltransferase catalytic subunit Dpm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 281 bits (690), Expect = 5e-77 Identities = 128/189 (67%), Positives = 156/189 (82%) Frame = +2 Query: 83 KYSILLPTYNERENLPIIIWLIIKYLDESLVDYEVIIIDDGSPDGTSEVARQLQKLYGSS 262 KYS+LLPTYNER+NLPII +LI K D+ +D+E++IIDD SPDGT EVA++LQK+YG Sbjct: 3 KYSVLLPTYNERKNLPIITYLIAKTFDQEKLDWEIVIIDDASPDGTQEVAKELQKIYGED 62 Query: 263 KIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDADLSHHPKFIPEFIKLQLKYDYDIVSG 442 KI+L+PR KLGLGTAYIHG++ A+G+F+IIMDAD SHHPK++PEFIKLQ +++YDIV G Sbjct: 63 KILLKPRSGKLGLGTAYIHGLKFATGDFVIIMDADFSHHPKYLPEFIKLQKEHNYDIVLG 122 Query: 443 TRYKGSGGVYGWDFKRKLISRGANFLTQLMLRPGVSDLTGSFRLYKKEVLEKLILSCVSK 622 TRY GGVYGW+ KRK ISRGAN L +L GVSD+TGSFRLYKK VLE L+ SK Sbjct: 123 TRYAKDGGVYGWNLKRKFISRGANLLASTVLGTGVSDVTGSFRLYKKPVLETLMSEVTSK 182 Query: 623 GYVFQMEMI 649 GYVFQME+I Sbjct: 183 GYVFQMEII 191 >SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 28.3 bits (60), Expect = 1.0 Identities = 8/21 (38%), Positives = 14/21 (66%) Frame = +1 Query: 289 EAWTRNCLHSWDTASFWKFYY 351 E + +NC WD++ FWK ++ Sbjct: 41 EKYAKNCNTIWDSSKFWKQFF 61 >SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 607 Score = 25.8 bits (54), Expect = 5.5 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +3 Query: 198 MMEALMGLQKWRVSCRSSMAPLK*CFDLAR*SLDSELPTF 317 M+ +G+QKW + S M+P D R DS +PT+ Sbjct: 452 MLSEEIGIQKWIIQSLSKMSPT--LNDFCR-RYDSSMPTY 488 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 25.8 bits (54), Expect = 5.5 Identities = 9/23 (39%), Positives = 16/23 (69%) Frame = +3 Query: 270 CFDLAR*SLDSELPTFMGYSKLL 338 C+D+ R +LD E PT+ ++L+ Sbjct: 213 CYDICRRNLDIERPTYENLNRLI 235 >SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 298 Score = 25.8 bits (54), Expect = 5.5 Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 3/65 (4%) Frame = +2 Query: 53 EVDSGLIKRDKYSILLPTYNERENLPIIIWLI---IKYLDESLVDYEVIIIDDGSPDGTS 223 ++D +IK K LP + N P + I K+L + ++VII D PDG + Sbjct: 119 DIDEDVIKVSKQ--YLPEMSAGFNHPKVKVHIGDGFKFLQDYQNTFDVIITDSSDPDGPA 176 Query: 224 EVARQ 238 E Q Sbjct: 177 EALFQ 181 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,650,553 Number of Sequences: 5004 Number of extensions: 53528 Number of successful extensions: 115 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 115 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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