BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0093 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g20575.1 68414.m02567 dolichyl-phosphate beta-D-mannosyltrans... 277 5e-75 At2g39630.2 68415.m04859 glycosyl transferase family 2 protein s... 58 5e-09 At2g39630.1 68415.m04858 glycosyl transferase family 2 protein s... 58 5e-09 At1g60710.1 68414.m06834 aldo/keto reductase family protein cont... 30 1.2 At3g56000.1 68416.m06222 glycosyl transferase family 2 protein s... 29 2.0 At2g27770.1 68415.m03365 expressed protein 29 2.7 >At1g20575.1 68414.m02567 dolichyl-phosphate beta-D-mannosyltransferase, putative / dolichol-phosphate mannosyltransferase, putative / mannose-P-dolichol synthase, putative similar to DPM1 from Homo sapiens [SP|O60762]; member of glycosyltransferase family 2 Length = 246 Score = 277 bits (679), Expect = 5e-75 Identities = 129/205 (62%), Positives = 163/205 (79%) Frame = +2 Query: 38 VVDSMEVDSGLIKRDKYSILLPTYNERENLPIIIWLIIKYLDESLVDYEVIIIDDGSPDG 217 + D ME K+ KYSI++PTYNER N+ II++LI K+L + VD+E+I++DDGSPDG Sbjct: 1 MADEMETKGE--KKYKYSIIIPTYNERLNIAIIVYLIFKHLRD--VDFEIIVVDDGSPDG 56 Query: 218 TSEVARQLQKLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDADLSHHPKFIPE 397 T E+ +QLQ+LYG +I+LR R KLGLGTAYIHG++ A+G+F++IMDADLSHHPK++P Sbjct: 57 TQEIVKQLQQLYGEDRILLRARAKKLGLGTAYIHGLKHATGDFVVIMDADLSHHPKYLPS 116 Query: 398 FIKLQLKYDYDIVSGTRYKGSGGVYGWDFKRKLISRGANFLTQLMLRPGVSDLTGSFRLY 577 FIK QL+ + IV+GTRY GGV+GW+ RKL SRGAN L Q +L PGVSDLTGSFRLY Sbjct: 117 FIKKQLETNASIVTGTRYVKGGGVHGWNLMRKLTSRGANVLAQTLLWPGVSDLTGSFRLY 176 Query: 578 KKEVLEKLILSCVSKGYVFQMEMII 652 KK LE +I SCVSKGYVFQMEMI+ Sbjct: 177 KKSALEDVISSCVSKGYVFQMEMIV 201 >At2g39630.2 68415.m04859 glycosyl transferase family 2 protein similar to dolichyl-phosphate beta-glucosyltransferase from Saccharomyces cerevisiae [SP|P40350]; contains Pfam glycosyltransferase group 2 domain PF00535 Length = 236 Score = 58.0 bits (134), Expect = 5e-09 Identities = 34/102 (33%), Positives = 52/102 (50%), Gaps = 6/102 (5%) Frame = +2 Query: 80 DKY-SILLPTYNERENLPIIIWLIIKYLDESL-----VDYEVIIIDDGSPDGTSEVARQL 241 +KY S+++P YNE LP + + YL + +EV+I+DDGS DGT VA Sbjct: 65 EKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDF 124 Query: 242 QKLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDAD 367 + Y I + P G G A G+ + G ++++DAD Sbjct: 125 IRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDAD 166 >At2g39630.1 68415.m04858 glycosyl transferase family 2 protein similar to dolichyl-phosphate beta-glucosyltransferase from Saccharomyces cerevisiae [SP|P40350]; contains Pfam glycosyltransferase group 2 domain PF00535 Length = 336 Score = 58.0 bits (134), Expect = 5e-09 Identities = 34/102 (33%), Positives = 52/102 (50%), Gaps = 6/102 (5%) Frame = +2 Query: 80 DKY-SILLPTYNERENLPIIIWLIIKYLDESL-----VDYEVIIIDDGSPDGTSEVARQL 241 +KY S+++P YNE LP + + YL + +EV+I+DDGS DGT VA Sbjct: 65 EKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDF 124 Query: 242 QKLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDAD 367 + Y I + P G G A G+ + G ++++DAD Sbjct: 125 IRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDAD 166 >At1g60710.1 68414.m06834 aldo/keto reductase family protein contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family Length = 345 Score = 30.3 bits (65), Expect = 1.2 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +2 Query: 65 GLIKRDKYSILLPTYNERENLPIIIW 142 G +K D+YS ++PT+ E P+ W Sbjct: 317 GFVKGDRYSNMIPTFKNAETPPLSAW 342 >At3g56000.1 68416.m06222 glycosyl transferase family 2 protein similar to beta-(1-3)-glucosyl transferase GB:AAC62210 GI:3687658 from [Bradyrhizobium japonicum], cellulose synthase from Agrobacterium tumeficiens [gi:710492] and Agrobacterium radiobacter [gi:710493]; contains Pfam glycosyl transferase, group 2 family protein domain PF00535 Length = 535 Score = 29.5 bits (63), Expect = 2.0 Identities = 29/109 (26%), Positives = 50/109 (45%), Gaps = 9/109 (8%) Frame = +2 Query: 92 ILLPTYNERENLPIIIWLIIKYLDESLVDYEVIIIDDGSPDGTSEVARQLQKLYGSSKIV 271 I +P +NE+E + I K L L + ++DD + + + ++ R K + S I Sbjct: 96 IQIPIFNEKEVCQLSIGAACK-LSWPLDRMIIQVLDDSTEEESQKLVRLECKKWESEGIT 154 Query: 272 LRP------RE-MKLGLGTAYI-HG-IQQASGNFIIIMDADLSHHPKFI 391 ++ RE K G TA + H + + F++I DAD P F+ Sbjct: 155 IKSEVRGGFREGFKAGALTAGMKHSYVDEYKCEFVVIFDADFQPEPDFL 203 >At2g27770.1 68415.m03365 expressed protein Length = 320 Score = 29.1 bits (62), Expect = 2.7 Identities = 28/81 (34%), Positives = 40/81 (49%), Gaps = 4/81 (4%) Frame = -3 Query: 249 SFCS*RATSEV---PSGLPS-SIMITS*STRLSSKYLIISHIIIGRFSRSL*VGSKIEYL 82 S CS +T+ V PS +PS ITS SK+LII + + G I Sbjct: 26 SSCSKYSTNNVCISPSLIPSIQTSITSIYRITLSKHLIIKVTWCNPHNNN---GLSISVA 82 Query: 81 SLFINPESTSMLSTTTQFYRK 19 S NP +T L+T+++F+RK Sbjct: 83 SADQNPSTTLKLNTSSRFFRK 103 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,436,531 Number of Sequences: 28952 Number of extensions: 265781 Number of successful extensions: 691 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 675 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 690 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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