SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0093
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g20575.1 68414.m02567 dolichyl-phosphate beta-D-mannosyltrans...   277   5e-75
At2g39630.2 68415.m04859 glycosyl transferase family 2 protein s...    58   5e-09
At2g39630.1 68415.m04858 glycosyl transferase family 2 protein s...    58   5e-09
At1g60710.1 68414.m06834 aldo/keto reductase family protein cont...    30   1.2  
At3g56000.1 68416.m06222 glycosyl transferase family 2 protein s...    29   2.0  
At2g27770.1 68415.m03365 expressed protein                             29   2.7  

>At1g20575.1 68414.m02567 dolichyl-phosphate
           beta-D-mannosyltransferase, putative /
           dolichol-phosphate mannosyltransferase, putative /
           mannose-P-dolichol synthase, putative similar to DPM1
           from Homo sapiens [SP|O60762]; member of
           glycosyltransferase family 2
          Length = 246

 Score =  277 bits (679), Expect = 5e-75
 Identities = 129/205 (62%), Positives = 163/205 (79%)
 Frame = +2

Query: 38  VVDSMEVDSGLIKRDKYSILLPTYNERENLPIIIWLIIKYLDESLVDYEVIIIDDGSPDG 217
           + D ME      K+ KYSI++PTYNER N+ II++LI K+L +  VD+E+I++DDGSPDG
Sbjct: 1   MADEMETKGE--KKYKYSIIIPTYNERLNIAIIVYLIFKHLRD--VDFEIIVVDDGSPDG 56

Query: 218 TSEVARQLQKLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDADLSHHPKFIPE 397
           T E+ +QLQ+LYG  +I+LR R  KLGLGTAYIHG++ A+G+F++IMDADLSHHPK++P 
Sbjct: 57  TQEIVKQLQQLYGEDRILLRARAKKLGLGTAYIHGLKHATGDFVVIMDADLSHHPKYLPS 116

Query: 398 FIKLQLKYDYDIVSGTRYKGSGGVYGWDFKRKLISRGANFLTQLMLRPGVSDLTGSFRLY 577
           FIK QL+ +  IV+GTRY   GGV+GW+  RKL SRGAN L Q +L PGVSDLTGSFRLY
Sbjct: 117 FIKKQLETNASIVTGTRYVKGGGVHGWNLMRKLTSRGANVLAQTLLWPGVSDLTGSFRLY 176

Query: 578 KKEVLEKLILSCVSKGYVFQMEMII 652
           KK  LE +I SCVSKGYVFQMEMI+
Sbjct: 177 KKSALEDVISSCVSKGYVFQMEMIV 201


>At2g39630.2 68415.m04859 glycosyl transferase family 2 protein
           similar to dolichyl-phosphate beta-glucosyltransferase
           from Saccharomyces cerevisiae [SP|P40350]; contains Pfam
           glycosyltransferase group 2 domain PF00535
          Length = 236

 Score = 58.0 bits (134), Expect = 5e-09
 Identities = 34/102 (33%), Positives = 52/102 (50%), Gaps = 6/102 (5%)
 Frame = +2

Query: 80  DKY-SILLPTYNERENLPIIIWLIIKYLDESL-----VDYEVIIIDDGSPDGTSEVARQL 241
           +KY S+++P YNE   LP  +   + YL +         +EV+I+DDGS DGT  VA   
Sbjct: 65  EKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDF 124

Query: 242 QKLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDAD 367
            + Y    I + P     G G A   G+  + G  ++++DAD
Sbjct: 125 IRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDAD 166


>At2g39630.1 68415.m04858 glycosyl transferase family 2 protein
           similar to dolichyl-phosphate beta-glucosyltransferase
           from Saccharomyces cerevisiae [SP|P40350]; contains Pfam
           glycosyltransferase group 2 domain PF00535
          Length = 336

 Score = 58.0 bits (134), Expect = 5e-09
 Identities = 34/102 (33%), Positives = 52/102 (50%), Gaps = 6/102 (5%)
 Frame = +2

Query: 80  DKY-SILLPTYNERENLPIIIWLIIKYLDESL-----VDYEVIIIDDGSPDGTSEVARQL 241
           +KY S+++P YNE   LP  +   + YL +         +EV+I+DDGS DGT  VA   
Sbjct: 65  EKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDF 124

Query: 242 QKLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDAD 367
            + Y    I + P     G G A   G+  + G  ++++DAD
Sbjct: 125 IRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDAD 166


>At1g60710.1 68414.m06834 aldo/keto reductase family protein
           contains Pfam profile PF00248: oxidoreductase, aldo/keto
           reductase family
          Length = 345

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = +2

Query: 65  GLIKRDKYSILLPTYNERENLPIIIW 142
           G +K D+YS ++PT+   E  P+  W
Sbjct: 317 GFVKGDRYSNMIPTFKNAETPPLSAW 342


>At3g56000.1 68416.m06222 glycosyl transferase family 2 protein
           similar to beta-(1-3)-glucosyl transferase GB:AAC62210
           GI:3687658 from [Bradyrhizobium japonicum], cellulose
           synthase from Agrobacterium tumeficiens [gi:710492] and
           Agrobacterium radiobacter [gi:710493]; contains Pfam
           glycosyl transferase, group 2 family protein domain
           PF00535
          Length = 535

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 29/109 (26%), Positives = 50/109 (45%), Gaps = 9/109 (8%)
 Frame = +2

Query: 92  ILLPTYNERENLPIIIWLIIKYLDESLVDYEVIIIDDGSPDGTSEVARQLQKLYGSSKIV 271
           I +P +NE+E   + I    K L   L    + ++DD + + + ++ R   K + S  I 
Sbjct: 96  IQIPIFNEKEVCQLSIGAACK-LSWPLDRMIIQVLDDSTEEESQKLVRLECKKWESEGIT 154

Query: 272 LRP------RE-MKLGLGTAYI-HG-IQQASGNFIIIMDADLSHHPKFI 391
           ++       RE  K G  TA + H  + +    F++I DAD    P F+
Sbjct: 155 IKSEVRGGFREGFKAGALTAGMKHSYVDEYKCEFVVIFDADFQPEPDFL 203


>At2g27770.1 68415.m03365 expressed protein
          Length = 320

 Score = 29.1 bits (62), Expect = 2.7
 Identities = 28/81 (34%), Positives = 40/81 (49%), Gaps = 4/81 (4%)
 Frame = -3

Query: 249 SFCS*RATSEV---PSGLPS-SIMITS*STRLSSKYLIISHIIIGRFSRSL*VGSKIEYL 82
           S CS  +T+ V   PS +PS    ITS      SK+LII        + +   G  I   
Sbjct: 26  SSCSKYSTNNVCISPSLIPSIQTSITSIYRITLSKHLIIKVTWCNPHNNN---GLSISVA 82

Query: 81  SLFINPESTSMLSTTTQFYRK 19
           S   NP +T  L+T+++F+RK
Sbjct: 83  SADQNPSTTLKLNTSSRFFRK 103


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,436,531
Number of Sequences: 28952
Number of extensions: 265781
Number of successful extensions: 691
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -