Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0087
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g39960.1 68415.m04910 microsomal signal peptidase 25 kDa subu... 50 2e-06
At1g51870.1 68414.m05847 protein kinase family protein contains ... 30 1.7
At1g51860.1 68414.m05846 leucine-rich repeat protein kinase, put... 30 1.7
At1g79880.3 68414.m09332 La domain-containing protein contains P... 29 2.9
At1g79880.2 68414.m09331 La domain-containing protein contains P... 29 2.9
At1g79880.1 68414.m09333 La domain-containing protein contains P... 29 2.9
At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family p... 29 3.8
At1g08150.1 68414.m00898 sodium/hydrogen exchanger family protei... 29 3.8
At1g51890.1 68414.m05849 leucine-rich repeat protein kinase, put... 28 5.0
At1g51880.1 68414.m05848 leucine-rich repeat protein kinase, put... 28 5.0
At5g59650.1 68418.m07479 leucine-rich repeat protein kinase, put... 28 6.7
At3g21340.1 68416.m02695 leucine-rich repeat protein kinase, put... 28 6.7
At1g51850.1 68414.m05845 leucine-rich repeat protein kinase, put... 28 6.7
At1g51805.1 68414.m05838 leucine-rich repeat protein kinase, put... 27 8.8
>At2g39960.1 68415.m04910 microsomal signal peptidase 25 kDa
subunit, putative (SPC25) identical to Probable
microsomal signal peptidase 25 kDa subunit (EC 3.4.-.-)
(SPase 25 kDa subunit) (SPC25) (Swiss-Prot:P58684)
[Arabidopsis thaliana]; contains non-consensus AT-AC
splice sites; contains 1 transmembrane domain;
Length = 192
Score = 49.6 bits (113), Expect = 2e-06
Identities = 44/186 (23%), Positives = 78/186 (41%), Gaps = 3/186 (1%)
Frame = +3
Query: 132 SETAEAAKINKWDGAAAKNAVDDAIREVMTGDLKCKESFALIDGRLFXXXXXXXXXXXXX 311
S K N D + K+ +D+++ +++T KE L + +L
Sbjct: 8 STNKNVKKANLLDHHSIKHILDESVSDIVTSR-GYKEDVRLSNLKLILGTIIIVVALVAQ 66
Query: 312 XWDYLYPFPQSRLVLIICVSSYFILMGILTLYTTLKEKGIFVVAKEKVGNNTRV-WEASS 488
Y FP++R LI C++ Y +L +L L KEK + G+ T SS
Sbjct: 67 F--YNKKFPENRDFLIGCIALYVVLNAVLQLILYTKEKNAILFTYPPEGSFTSTGLVVSS 124
Query: 489 YVKKHDDKYNLVIVMRDTNGNTREASV--TKSFANFIDVNGTVVQNIVSNEITKLYHSLS 662
+ + D+Y L I D + SV TKS + +G +V+ + ++ L + +
Sbjct: 125 KLPRFSDQYTLTIDSADPKSISAGKSVQLTKSVTQWFTKDGVLVEGLFWKDVEALIKNYA 184
Query: 663 SEKKEK 680
E+ +K
Sbjct: 185 EEEPKK 190
>At1g51870.1 68414.m05847 protein kinase family protein contains
Serine/Threonine protein kinases active-site signature,
PROSITE:PS00108
Length = 837
Score = 29.9 bits (64), Expect = 1.7
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +2
Query: 293 CGPLRTAMGLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
CG GLS S P G H+ ++ T GY DP +Y
Sbjct: 667 CGAKLADFGLSRSFPI--DGECHVSTVVAGTPGYLDPEYY 704
>At1g51860.1 68414.m05846 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 890
Score = 29.9 bits (64), Expect = 1.7
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +2
Query: 293 CGPLRTAMGLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
CG GLS S P G H+ ++ T GY DP +Y
Sbjct: 720 CGAKLADFGLSRSFPI--DGECHVSTVVAGTPGYLDPEYY 757
>At1g79880.3 68414.m09332 La domain-containing protein contains Pfam
profile PF05383: La domain; similar to putative protein
GB:CAA18589 [Arabidopsis thaliana]
Length = 357
Score = 29.1 bits (62), Expect = 2.9
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +3
Query: 432 FVVAKEKVGNNTRVWEASSYVKKHDDKYNLVIVMRDTNGNTREASVTKSFANFIDVNGTV 611
++ ++EKV N + + +K+ +DK + NG+ AS+ K + + V
Sbjct: 171 WIASEEKVTNKEKPSALKNKIKEKEDKETGIADREKENGDNSCASLCKDNTDQLVVPPWN 230
Query: 612 VQNIVSNEITK-LYHSLSSEKKEKY 683
N VS+E+ K L+ S + +Y
Sbjct: 231 NSNSVSSEVLKDLFQRFGSVEHIEY 255
>At1g79880.2 68414.m09331 La domain-containing protein contains Pfam
profile PF05383: La domain; similar to putative protein
GB:CAA18589 [Arabidopsis thaliana]
Length = 345
Score = 29.1 bits (62), Expect = 2.9
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +3
Query: 432 FVVAKEKVGNNTRVWEASSYVKKHDDKYNLVIVMRDTNGNTREASVTKSFANFIDVNGTV 611
++ ++EKV N + + +K+ +DK + NG+ AS+ K + + V
Sbjct: 171 WIASEEKVTNKEKPSALKNKIKEKEDKETGIADREKENGDNSCASLCKDNTDQLVVPPWN 230
Query: 612 VQNIVSNEITK-LYHSLSSEKKEKY 683
N VS+E+ K L+ S + +Y
Sbjct: 231 NSNSVSSEVLKDLFQRFGSVEHIEY 255
>At1g79880.1 68414.m09333 La domain-containing protein contains Pfam
profile PF05383: La domain; similar to putative protein
GB:CAA18589 [Arabidopsis thaliana]
Length = 399
Score = 29.1 bits (62), Expect = 2.9
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +3
Query: 432 FVVAKEKVGNNTRVWEASSYVKKHDDKYNLVIVMRDTNGNTREASVTKSFANFIDVNGTV 611
++ ++EKV N + + +K+ +DK + NG+ AS+ K + + V
Sbjct: 225 WIASEEKVTNKEKPSALKNKIKEKEDKETGIADREKENGDNSCASLCKDNTDQLVVPPWN 284
Query: 612 VQNIVSNEITK-LYHSLSSEKKEKY 683
N VS+E+ K L+ S + +Y
Sbjct: 285 NSNSVSSEVLKDLFQRFGSVEHIEY 309
>At5g38460.1 68418.m04649 ALG6, ALG8 glycosyltransferase family
protein similar to SP|Q9Y672 Dolichyl pyrophosphate
Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC 2.4.1.-)
(Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl
glucosyltransferase) {Homo sapiens}; contains Pfam
profile PF03155: ALG6, ALG8 glycosyltransferase family
Length = 533
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3
Query: 327 YPFPQSRLVLIICVSSYFILMGILTLYT 410
YPF L++I+C SYFI+ T YT
Sbjct: 489 YPFLFEALIMILCF-SYFIMFAFYTNYT 515
>At1g08150.1 68414.m00898 sodium/hydrogen exchanger family protein
contains InterPro entry IPR006153: Sodium/hydrogen
exchanger
Length = 815
Score = 28.7 bits (61), Expect = 3.8
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +3
Query: 315 WDYLYPFPQSRLVLIICVSSYFIL 386
WDY P +S +VL++C+ +F L
Sbjct: 61 WDYSLPHLESVIVLVLCLWQFFYL 84
>At1g51890.1 68414.m05849 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 888
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 317 GLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
GLS S P G +H+ ++ T GY DP +Y
Sbjct: 726 GLSRSFPV--DGESHVMTVVAGTPGYLDPEYY 755
>At1g51880.1 68414.m05848 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 880
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 317 GLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
GLS S P G +H+ ++ T GY DP +Y
Sbjct: 718 GLSRSFPV--DGESHVSTVVAGTPGYLDPEYY 747
>At5g59650.1 68418.m07479 leucine-rich repeat protein kinase,
putative contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 892
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 317 GLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
GLS S P G H+ ++ T GY DP +Y
Sbjct: 732 GLSRSFPI--GGETHISTVVAGTPGYLDPEYY 761
>At3g21340.1 68416.m02695 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 880
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 317 GLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
GLS S P G H+ ++ T GY DP +Y
Sbjct: 718 GLSRSFPI--EGETHVSTVVAGTPGYLDPEYY 747
>At1g51850.1 68414.m05845 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376
Length = 865
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 317 GLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
GLS S P G H+ ++ T GY DP +Y
Sbjct: 703 GLSRSFPI--EGETHVSTVVAGTPGYLDPEYY 732
>At1g51805.1 68414.m05838 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 884
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 317 GLSLSIPSIKTGSNHLRVIIFHTDGYFDPLHY 412
GLS S P G H+ ++ T GY DP +Y
Sbjct: 722 GLSRSFPI--GGETHVSTVVAGTPGYLDPEYY 751
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,271,544
Number of Sequences: 28952
Number of extensions: 287765
Number of successful extensions: 705
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 688
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 705
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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